检索结果-南通市图书馆

Genome-wide association study reveals that JASMONATE zim.DOMAIN 5 regulates seed germination in rice

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2024年第4期12卷 1001-1009页

作者： Shan Sun Liling Peng Qianqian Huang Zhibo Huang Chengjing Wang Jia zhao Zhoufei Wang Yongqi He The Laboratory of Seed Science and Technology Guangdong Key Laboratory of Plant Molecular BreedingKey Laboratory for Enhancing Resource Use Efficiency of Crops in South ChinaMinistry of Agriculture and Rural AffairsSouth China Agricultural UniversityGuangzhou 510642GuangdongChina

Seed germination is a complex trait regulated by multiple genes in ***,the regulators of rice seed germination have yet to be sufficiently ***,a quantitative trait locus(QTL)for rice seed germination was identified in a genome-wide association *** candidate gene JASMONATE zim.DOMAIN 5(OsJAZ5)of the QTL was verified that positively regulates seed ***5 regulation of seed germination involves an OsABI3-mediated abscisic acid *** of OsJAZ5 facilitated seed *** application of OsJAZ5 might be useful for increasing seed germination for rice direct seeding.

关键词： Abscisic acid JASMONATE zim.DOMAIN Oryza sativa Seed germination

玉米Zmzim.族基因鉴定及其对氮素的响应特征

在线全文

学校读者我要写书评

暂无评论

江苏农业学报 2024年第4期40卷 577-590页

作者：孙扬名张明亮葛敏邬奇赵涵南京农业大学农学院江苏南京210095 江苏省农业科学院种质资源与生物技术研究所江苏南京210014

为明确玉米Zmzim.族基因结构、位置、编码蛋白质性质及其对氮素的响应特征,本研究利用TBtools、MEGA X等软件分析玉米Zmzim.族基因的结构、染色体位置、顺式作用元件及系统发育关系及其编码蛋白质的理化性质、保守结构域及基序,结合... 详细信息

为明确玉米Zmzim.族基因结构、位置、编码蛋白质性质及其对氮素的响应特征,本研究利用TBtools、MEGA X等软件分析玉米Zmzim.族基因的结构、染色体位置、顺式作用元件及系统发育关系及其编码蛋白质的理化性质、保守结构域及基序,结合玉米不同发育时期不同器官的转录组数据及充足氮与低氮水平下四叶一心期玉米地上部转录组数据解析玉米Zmzim.族基因的表达模式及差异。结果表明:从玉米全基因组中共鉴定到32个玉米Zmzim.因,主要分布于1号、2号、5号和7号染色体,8号和10号染色体上无Zmzim.因。32个Zmzim.因可划分为4个亚类,其编码蛋白质由134~467个氨基酸残基构成,均为亲水性蛋白质且全部定位在细胞核中。32个Zmzim.因启动子区域顺式作用元件主要有调控元件、光信号响应元件、激素信号响应元件、胁迫响应元件、生长发育元件及蛋白质结合位点等6大类。不同发育时期,Zmzim.因在玉米不同器官中存在差异性表达;在充足氮与低氮处理下,随着处理时间的增加,玉米植株地上部12个Zmzim.因无表达或相对表达量较低,6个Zmzim.因相对表达量较高且稳定,其余的14个Zmzim.因的相对表达量差异较大;Zmzim.、Zmzim.6、和Zmzim.13个基因的相对表达量普遍高于其他基因。充足氮条件下,Zmzim.、Zmzim.5、Zmzim.0、Zmzim.4、Zmzim.9和Zmzim.1基因的相对表达量普遍高于低氮条件。本研究结果为玉米氮高效吸收利用基因筛选和利用奠定基础。

关键词：玉米 zim.录因子基因家族分析氮响应

维普期刊数据库博看期刊

玉米ZmMAPKKK21基因的克隆和生物信息学分析

在线全文

学校读者我要写书评

暂无评论

作物杂志 2024年第2期 30-39页

作者：张倩任雯赵冰兵周秒依李韩帅刘亚杜何为长江大学生命科学学院湖北荆州434025 北京市农林科学院玉米研究所/玉米DNA指纹及分子育种北京重点实验室北京100097

丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)在植物应对生物和非生物胁迫过程中发挥着重要作用。在前期研究中我们挖掘到ZmMAPKKK21这一潜在的重要抗旱基因,本研究从玉米抗旱自交系J24中克隆获得ZmMAPKKK21基因,并对其... 详细信息

丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)在植物应对生物和非生物胁迫过程中发挥着重要作用。在前期研究中我们挖掘到ZmMAPKKK21这一潜在的重要抗旱基因,本研究从玉米抗旱自交系J24中克隆获得ZmMAPKKK21基因,并对其进行生物信息学分析。结果表明,玉米ZmMAPKKK21基因开放阅读框长1419 bp,编码472个氨基酸,是不具有信号肽的亲水性蛋白,且其启动子序列含有多个与逆境胁迫和激素有关的顺式作用元件;玉米中的MAPKKK21蛋白预测定位在细胞核上,蛋白互作预测结果显示,ZmMAPKKK21蛋白与参与植物逆境胁迫相关的MAPKK3蛋白和zim.族蛋白发生互作,其结构与高粱(Sorghum bicolor L.)和谷子(Setaria italica L.)等高抗旱禾本科作物相比,不但在STKc_MAPKKK结构域上高度保守,且具有更加相似的二级结构和三级结构;qRT-PCR分析发现,ZmMAPKKK21基因在玉米根系中表达水平较高,干旱胁迫后该基因在根和叶中表达上调,进一步验证了ZmMAPKKK21基因的表达与干旱胁迫响应的相关性。

关键词：玉米 MAPKKK 生物信息学分析表达分析

在线全文

学校读者我要写书评

暂无评论

成年病人经下肢行PICC的研究进展

护理研究 2024年第3期38卷 432-438页

作者：刘春丽章毛毛于蓉李晓旭邾萍薛慧徐礼勇赵云张柳柳江苏省肿瘤医院江苏省肿瘤防治研究所南京医科大学附属肿瘤医院江苏210009 南京农业大学医院

就成年病人经下肢对外周静脉穿刺中心静脉置管(PICC)适应证、禁忌证、应用区域选择法(zim.技术优化置管穿刺部位,应用隧道、超声下不同平面穿刺技术以及心电图(ECG)定位下腔静脉系统内导管尖端位置等进行综述,以期为临床实践提供参考。

关键词：经外周静脉穿刺置入中心静脉导管下肢股静脉股浅静脉心电图隧道综述

The SlWRKY57-SlVQ21/SlVQ16 module regulates salt stress in tomato

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2023年第11期65卷 2437-2455页

作者： Jilin Ma Chonghua Li Lulu Sun Xuechun Ma Hui Qiao Wenchao zhao Rui Yang Susheng Song Shaohui Wang Huang Huang Plant Science and Technology College Beijing University of AgricultureBeijing 102206China Beijing Key Laboratory for Agricultural Application and New Technique Beijing University of AgricultureBeijing 102206China College of Life Sciences Capital Normal UniversityBeijing 100048China

Salt stress is a major abiotic stress which severely hinders crop ***,the regulatory network controlling tomato resistance to salt remains ***,we found that the tomato WRKY transcription factor WRKY57 acted as a negative regulator in salt stress response by directly attenuating the transcription of salt-responsive genes(Sl RD29B and Sl DREB2)and an ion homeostasis gene(Sl SOS1).We further identified two VQ-motif containing proteins Sl VQ16 and Sl VQ21as Sl WRKY57-interacting *** VQ16 positively,while Sl VQ21 negatively modulated tomato resistance to salt *** VQ16 and Sl VQ21 competitively interacted with Sl WRKY57 and antagonistically regulated the transcriptional repression activity of Sl ***,the Sl WRKY57-Sl VQ21/Sl VQ16 module was involved in the pathway of phytohormone jasmonates(JAs)by interacting with JA repressors JA-zim.domain(JAZ)*** results provide new insights into how the Sl WRKY57-Sl VQ21/Sl VQ16 module finely tunes tomato salt tolerance.

关键词： jasmonates salt stress tomato VQ protein WRKY transcription factor

The boron transporter SiBOR1 functions in cell wall integrity, cellular homeostasis, and panicle development in foxtail millet

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2022年第2期10卷 342-353页

作者： Hailong Wang Sha Tang Hui Zhi Lihe Xing Haoshan Zhang Chanjuan Tang Enbo Wang Meicheng zhao Guanqing Jia Baili Feng Xianmin Diao State Key Laboratory of Crop Stress Biology for Arid Areas College of AgronomyNorthwest A&F UniversityYangling 712000ShaanxiChina Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China

Boron(B) is an essential micronutrient for vascular plant growth. Both B deficiency and toxicity can impair tissue development in diverse plant species, but little is known about the effect of B on reproductive panicle development and grain yield. In this study, a mutant of Setaria italica exhibiting necrotic panicle apices was identified and designated as sibor1. Sequencing revealed a candidate gene, Si BOR1, with a G-to-A alteration at the seventh exon. Knockout transgenic lines generated by clustered regularly interspaced short palindromic repeats and their associated protein-9 also had necrotic panicles, verifying the function of Si BOR1. Si BOR1 encoded a membrane-localized B efflux transporter, co-orthologous to the rice BOR1 protein. Si BOR1 was dominantly expressed in panicles and displayed a distinct expression pattern from those of its orthologs in other species. The induced mutation in Si BOR1 caused a reduction in the B content of panicle primary branches, and B deficiency-associated phenotypes such as thicker cell walls and higher cell porosity compared with Yugu 1. Transcriptome analysis indicated that differentially expressed genes involved in cell wall biogenesis, jasmonic acid synthesis, and programmed cell death response pathways were enriched in sibor1. q PCR analysis identified several key genes, including phenylalanine ammonia-lyase(Si PAL) and jasmonate-zim.domain(Si JAZ) genes, responsive to B-deficient conditions. These results indicate that Si BOR1 helps to regulate panicle primary branch development to maintain grain yield in S. italica. Our findings shed light on molecular mechanisms underlying the relationship between B transport and plant development in S. italica.

关键词： Foxtail millet(Setaria italica) SiBOR1 Cell wall Panicle development Grain yield

Clone of Chinese Jinan red-cross yellow cattle and evaluation of reproductive characteristics of cloned calf

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2005年第22期50卷 2592-2597页

作者： DAI Yunping LIAN Zhengxing ZHU Huabin GONG Guochun WANG Lili WANG Haiping zhao Zhihui ZHU Qinghong FEI Jing LI Ning State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China Coltege of Animal Science and Technology China Agricultural University Beijing 100094 China Institute of Animal Science Chinese Acaderay of Agriculture Science Beijing 100094 China

Somatic cell clone technology is a viable ap- proach to preserving endangered livestock and wildlife ge- netic resources. In the present research, somatic cell nuclear transfer (SCNT) was performed using granulose cells from the critical endangered Chinese red-cross yellow cattle as donor cells. A total of 211 oocytes were manipulated and 166 (79%) of them were successfully enucleated. 112 (67.4%) SCNT embryos were reconstructed, 94 (83%) of them cleaved, and 48 (43%) of them developed to blastocyst stage. SCNT blastocysts were transferred to 6 Holstein recipients, and 2 (33%) of them were found to be pregnant. One of them maintained to term and delivered a calf, whereas another aborted. Effect of different fusion buffer (mannitol vs. zim. merman fusion buffer) and different activation methods (calcium ionophore+6-DMAP vs. cycloheximide+CB) on fu- sion rate and development of SCNT embryos were investi- gated. The results indicated that: (i) on condition of two DC pulses of 2.5 kV/cm for 10 μs each, fusion rates were higher in mannitol solution than in zim.erman fusion buffer (71% vs. 61%, respectively, p0.05 ); (ii) There was no significant difference in development rates to the blastocyst stage for SCNT embryos activated by calcium ionophore+6-DMAP or by cycloheximide+CB (42% vs. 46%, respectively, p>0.05). Microsatellite DNA analysis examining 28 loci confirmed that the cloned calf was genetically identi- cal to the donor Jinan red-cross yellow cattle and different from the recipient females. Growth and reproductive per- formance of cloned cow were evaluated, and there were no difference i cross-red n it between cloned and normal control Jinan yellow cattle. Furthermore, the cloned yellow cow has delivered a healthy yellow calf.

关键词：牛繁殖技术肉体细胞克隆技术遗传基因

ATTENTION TO VOCABULARY DEVELOPMENT IN READING:QUANTITY AND QUALITY

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Applied Linguistics 2000年第2期23卷 11-13页

作者： zhao Jizheng Zhanjiang Normal College

It has long been argued that little or no classroomattention is given to vocabulary(Carter,1987;zim.merman,1997),whereas the opposite might be saidof Chinese tertiary English *** problems stillremain:Does more time spent on vocabulary teachingand learning prove effective?Does more attentionneed to be paid to the quality of teaching and learningof vocabulary?To answer these questions,I argue inthis article for a balance of quality and quantity of at-tention to vocabulary *** the first partof the article,I present five common procedures invocabulary teaching and learning in Chinese collegesand universities and analyse the reasons for the low ef-ficiency in vocabulary teaching and *** thesecond,I put forward three techniques—a semanticmapping activity,creating meaningful contexts andusing an integrated approach in teaching and learningvocabulary.

关键词： EFL ATTENTION TO VOCABULARY DEVELOPMENT IN READING ESL

紫杉醇肿瘤靶向给药系统对MCF-7细胞的抑制作用观察

在线全文

学校读者我要写书评

暂无评论

郑州大学学报（医学版） 2013年第5期48卷 595-598页

作者：张艳艳张晓艳付旭东刘康栋赵继敏董子明张振中郑州大学基础医学院病理生理学教研室郑州450001 郑州大学第五附属医院神经外科郑州450052 郑州大学药学院郑州450001

目的:制备紫杉醇肿瘤靶向给药系统NGR-SWCNTs-Paclitaxel,观察其对MCF-7乳癌细胞的影响。方法:实验分为对照组、Paclitaxel组、SWCNTs-Paclitaxel组和NGR-SWCNTs-Paclitaxel组。用荧光物质FITC标记单壁碳纳米管(SWCNTs),采用细胞吞噬实验观察MCF-7细胞对各制剂的体外摄取情况,用MTT法观察各制剂对MCF-7细胞的增殖抑制作用,采用流式细胞术检测细胞周期和凋亡率。结果:MCF-7细胞能够有效吞噬NGR-SWCNTs-Paclitaxel复合物,未经NGR-SWCNTs修饰的Paclitaxel较少进入肿瘤细胞。Paclitaxel、SWCNTs-Paclitaxel和NGR-SWCNTs-Paclitaxel组的细胞增殖抑制率、细胞周期和细胞凋亡率差异均有统计学意义(P<0.05);NGR-SWCNTs-Paclitaxel对细胞的增殖抑制作用最强,诱导的细胞凋亡最有效,G2/M期阻滞效果也最显著(P<0.05)。结论:成功制备的NGR-SWCNTs-Paclitaxel复合物有良好的肿瘤靶向性和乳癌细胞增殖抑制作用。

关键词：紫杉醇单壁碳纳米管 NGR MCF-7细胞