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Nonlinear dynamics of a circular curved cantilevered pipe conveying pulsating fluid based on the geometrically exact model

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Applied Mathematics and Mechanics(English Edition) 2024年第2期45卷 261-276页

作者： Runqing CAO zilong guo Wei CHEN Huliang DAI Lin WANG Department of Engineering Mechanics School of Aerospace EngineeringHuazhong University of Science and TechnologyWuhan 430074China Hubei Key Laboratory for Engineering Structural Analysis and Safety Assessment Wuhan 430074China State Key Laboratory of Intelligent Manufacturing Equipment and Technology School of Mechanical Science and EngineeringHuazhong University of Science and TechnologyWuhan 430074China

Due to the novel applications of flexible pipes conveying fluid in the field of soft robotics and biomedicine,the investigations on the mechanical responses of the pipes have attracted considerable attention.The fluid-structure interaction(FSI)between the pipe with a curved shape and the time-varying internal fluid flow brings a great challenge to the revelation of the dynamical behaviors of flexible pipes,especially when the pipe is highly flexible and usually undergoes large deformations.In this work,the geometrically exact model(GEM)for a curved cantilevered pipe conveying pulsating fluid is developed based on the extended Hamilton's principle.The stability of the curved pipe with three different subtended angles is examined with the consideration of steady fluid flow.Specific attention is concentrated on the large-deformation resonance of circular pipes conveying pulsating fluid,which is often encountered in practical engineering.By constructing bifurcation diagrams,oscillating shapes,phase portraits,time traces,and Poincarémaps,the dynamic responses of the curved pipe under various system parameters are revealed.The mean flow velocity of the pulsating fluid is chosen to be either subcritical or supercritical.The numerical results show that the curved pipe conveying pulsating fluid can exhibit rich dynamical behaviors,including periodic and quasi-periodic motions.It is also found that the preferred instability type of a cantilevered curved pipe conveying steady fluid is mainly in the flutter of the second mode.For a moderate value of the mass ratio,however,a third-mode flutter may occur,which is quite different from that of a straight pipe system.

关键词： curved pipe conveying fluid pulsating fluid geometrically exact model(GEM) nonlinear dynamics parametric vibration flutter

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Theoretical study of the nonlinear force-loading control in single-molecule stretching experiments

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Communications in Theoretical Physics 2024年第4期76卷 167-175页

作者： Xingyu Qi zilong guo Shimin Le Hu Chen Research Institute for Biomimetics and Soft Matter Fujian Provincial Key Lab for Soft Functional Materials ResearchDepartment of PhysicsXiamen UniversityXiamen 361005China Center of Biomedical Physics Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhou 325000China

Force spectrum measurements with constant loading rates are widely used in single-molecule manipulation experiments to study the mechanical stability and force response of biomolecules.Force-dependent transition rates can be obtained from the transition force distribution,but it is limited to the force range with non-zero force distribution.Although constant loading rate control can be realized with magnetic tweezers,the loading rate range is limited due to the slow movement of permanent magnets.Non-linear exponential and exponential squared force loading functions are more feasible in magnetic tweezers,while there is no theoretical result available for these two kinds of non-linear force loading functions.In this study,we solved the unfolding process of a protein following Bell's model under nonlinear exponential and exponential squared force loading functions,which offer a broader range of unfolding force distribution compared to the traditional constant loading rate experiments.Furthermore,we derived two force loading functions,which can produce uniform unfolding force distribution.This research contributes fundamental equations for the analysis of experimental data obtained through single-molecule manipulation under nonlinear force loading controls,paving the way for the use of nonlinear force control in magnetic tweezer experiments.

关键词： single-molecule manipulation magnetic tweezers force loading transition rates force distribution

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Dynamic analysis and regulation of the flexible pipe conveying fluid with a hard-magnetic soft segment

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Applied Mathematics and Mechanics(English Edition) 2022年第9期43卷 1415-1430页

作者： zilong guo Qiao NI Wei CHEN Huliang DAI Lin WANG Department of Mechanics Huazhong University of Science and TechnologyWuhan 430074China Hubei Key Laboratory for Engineering Structural Analysis and Safety Assessment Wuhan 430074China State Key Lab of Digital Manufacturing Equipment and Technology School of Mechanical Science and EngineeringHuazhong University of Science and TechnologyWuhan 430074China State Key Laboratory of Structural Analysis for Industrial Equipment Dalian University of TechnologyDalian 116024Liaoning ProvinceChina

The recently developed hard-magnetic soft(HMS)materials can play a significant role in the actuation and control of medical devices,soft robots,flexible electronics,etc.To regulate the mechanical behaviors of the cantilevered pipe conveying fluid,the present work introduces a segment made of the HMS material located somewhere along the pipe length.Based on the absolute node coordinate formulation(ANCF),the governing equations of the pipe conveying fluid with an HMS segment are derived by the generalized Lagrange equation.By solving the derived equations with numerical methods,the static deformation,linear vibration characteristic,and nonlinear dynamic response of the pipe are analyzed.The result of the static deformation of the pipe shows that when the HMS segment is located in the middle of the pipe,the downstream portion of the pipe centerline will keep a straight shape,providing that the pipe is stable with a relatively low flow velocity.Therefore,it is possible to precisely regulate the ejection direction of the fluid flow by changing the magnetic and fluid parameters.It is also found that the intensity and direction of the external magnetic field greatly affect the stability and dynamic response of the pipe with an HMS segment.In most cases,the magnetic actuation increases the critical flow velocity for the flutter instability of the pipe system and suppresses the vibration amplitude of the pipe.

关键词： hard-magnetic soft(HMS)material pipe conveying fluid absolute node coordinate formulation(ANCF) stability dynamic response regulation

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A Miniature Fiber Tip Polystyrene Microsphere Temperature Sensor With High Sensitivity

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Photonic Sensors 2022年第1期12卷 84-90页

作者： Haibin CHEN Tianchong XIE Jiashuang FENG Xiongxing ZHANG Wei WANG Yanping LI zilong guo School of Optoelectronic Engineering Xi'an Technological UniversityXi'an 710021China Shaanxi Province Key Lab of Photoelectric Measurement and Instrument Technology Xi'an Technological UniversityXian 710021China

A fiber-optic temperature sensor based on fiber tip polystyrene microsphere is proposed.The sensor structure can be formed simply by placing and fixing a polystyrene microsphere on the center of an optical fiber tip.Since polystyrene has a much larger thermal expansivity,the structure can be used for high-sensitive temperature measurement.By the illuminating of the sensor with a broadband light source and through the optical Fabry-Perot interference between the front and back surfaces of the polystyrene microsphere,the optical phase difference(OPD)or wavelength shift can be used for the extraction of temperature.Temperature measurement experiment shows that,using a fiber probe polystyrene microsphere temperature sensor with a spherical diameter of about 91.7 pm,a high OPD-temperature sensitivity of about-0.61796nm/℃and a good linearity of 0.9916 were achieved in a temperature range of 20℃-70℃.

关键词： Polystyrene microsphere fiber-optic sensor Fabry-Perot interference temperature measurement

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REPUBLISHED TEXTS IN THE ATTIC ORATORS

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Journal of Ancient Civilizations 2021年第2期36卷 139-172,299页

作者： zilong guo IHAC NENUChangchun

In the present article,I explore the passages that are reused in the Attic orators,particularly those as transmitted in the judicial speeches by Antiphon and Demosthenes.I argue that the favorable reactions from their audiences or clients in the past legal cases are what encourage the orators to reuse,or republish,the passages,and that there is a tendency among them to make modifications to suit changing socio-political circumstances and specific performative contexts.The conclusion is that the republished texts highlight the common practice of the logographers in Classical Athens,and thus contribute to our understanding of their habitual way of writing and method of self-promotion.

关键词： legal promotion conclusion

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Mutation in a non-force-bearing region of protein L influences force-dependent unfolding behavior

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Chinese Physics B 2024年第7期33卷 624-629页

作者：蒋环杰王艳伟陈家媛胡丹潘海郭子龙陈虎 Department of Physics Wenzhou UniversityWenzhou 325035China Center of Biomedical Physics Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhou 325000China Research Institute for Biomimetics and Soft Matter Fujian Provincial Key Laboratory for Soft Functional Materials ResearchDepartment of PhysicsXiamen UniversityXiamen 361005China

Single-molecule magnetic tweezers(MTs) have revealed multiple transition barriers along the unfolding pathway of several two-state proteins, such as GB1 and Csp. In this study, we utilized MTs to measure the force-dependent folding and unfolding rates of both protein L(PLWT) and its Y47W mutant(PLY47W) where the mutation point is not at the force-bearing β-strands. The measurements were conducted within a force range of 3–120 pN. Notably, the unfolding rates of both PLWT and PWY47W exhibit distinct force sensitivities below 50 pN and above 60 pN, implying a two-barrier free energy landscape. Both PLWT and PLY47W share the same force-dependent folding rate and the same transition barriers,but the unfolding rate of PLY47W is faster than that of PLWT. Our finding demonstrates that the residue outside of the force-bearing region will also affect the force-induced unfolding dynamics.

关键词： protein folding magnetic tweezers protein L

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Force-dependent unfolding and folding dynamics of protein alpha-catenin modulation domains

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Journal of Innovative Optical Health Sciences 2019年第1期12卷 22-28页

作者： Xueping Li Weili Zhai zilong guo Hu Chen Research Institute for Biomimetics and Soft Matter Fujian Provincial Key Lab for Soft Functional Materials Research Department of Physics Xiamen UniversityXiamenChina

α-catenin is an adhesion protein located at the cadherin-based cell-cell adherens junction.α-catenin cross-linksβ-catenin and actin fiber in the adhesion protein complex,and plays an important role in the formation and modulation of cell-cell adhesion.The central modulation domains can be unfolded to expose binding site of vinculin when stretching force is applied.Here,we studied the force-induced unfolding dynamics ofα-catenin modulation domains under different loading rates from which the unfolding distance of M2 and M3 domains is determined to be 5-7 nm,and an unfolding intermediate state is identified.We also found that the folding process of M1-M3 domains goes through different pathways with cooperativity.

关键词： α-catenin folding and unfolding magnetic tweezer Bell's model

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A Novel Integrated Method for Large-Scale Detection, Identification, and Quantification of Widely Targeted Metabolites： Application in the Study of Rice Metabolomics

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Molecular Plant 2013年第6期6卷 1769-1780页

作者： Wei Chen Liang Gong zilong guo Wensheng Wang Hongyan Zhang Xianqing Liu SibinYu Lizhong Xiong Jie Luo National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Huazhong Agricultural University Wuhan430070 China Key Laboratory of Horticultural Plant Biology (Ministry of Education) Huazhong Agricultural University Wuhan 430070 China College of Life Science and Technology Huazhong Agricultural University Wuhan 430070 China

Liquid chromatography-mass spectrometry （LC-MS）-based metabolomics has been facilitated by the con- struction of MSz spectral tag （MS2T） library from the total scan ESI MS/MS data, and the development of widely targeted metabolomics method using MS/MS data gathered from authentic standards. In this report, a novel strategy called step- wise multiple ion monitoring-enhanced product ions （stepwise MIM-EPI） was developed to construct the MS2T library, in which stepwise MIM was used as survey scans to trigger the acquisition of EPI. A total number of 698 （almost） non- redundant metabolites with MS2 spectra were obtained, of which 135 metabolites were identified/annotated. Integrating the data gathered from our MS2T library and other available multiple reaction monitoring （MRM） information, a widely targeted metabolomics method was developed to quantify 277 metabolites, including some phytohormones. Evaluation of the dehydration responses and natural variations of these metabolites in rice leaf not only suggested the coordinated regulation of abscisic acid （ABA） with metabolites such as serotonin derivative（s）, polyamine conjugates under drought stress, but also revealed some C-glycosylated flavones as the potential markers for the discrimination of indica and japonica rice subspecies. The new MS2T library construction and widely targeted metabolomics strategy could be used as a tool for rice functional genomics.

关键词： MS2T library widely targeted metabolic profiling flavonoid rice.

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Natural Variation in the Sequence of SNAC1 and Its Expression Level Polymorphism in Rice Germplasms under Drought Stress

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Journal of Genetics and Genomics 2014年第11期41卷 609-612页

作者： Khamdok Songyikhangsuthor zilong guo Nili Wang Xiaoyi Zhu Weibo Xie Tongmin Mou Lizhong Xiong National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University

Water is a major limiting factor for food production and many countries fail to produce sufficient food for their population due to severe water scarcity （Jury and Vaux, 2005）. Rice is the main staple food worldwide. More than 50% of rice in the world is rain-fed and drought causes severe reduction in rice grain yield in rain-fed environments （Venuprasad et al., 2007; Zhang, 2007; Sandhu et al., 2014）. Therefore, enhancing drought resistance （DR） of rice is important for food security. However, DR is a complex trait, which is controlled by a large number of loci with small effect and is also affected by different genetic background, genotype-by-environment interaction and other stresses such as heat （Hu and Xiong, 2014）.

关键词： SNAC Natural Variation in the Sequence of SNAC1 and Its Expression Level Polymorphism in Rice Germplasms under Drought Stress

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Protein folding mechanism revealed by single-molecule force spectroscopy experiments

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Biophysics Reports 2021年第5期7卷 399-412页

作者： Hao Sun zilong guo Haiyan Hong Ping Yu Zhenyong Xue Hu Chen Research Institute for Biomimetics and Soft Matter Fujian Provincial Key Lab for Soft Functional Materials Research Department of Physics Xiamen University Center of Biomedical Physics Wenzhou Institute University of Chinese Academy of Sciences Oujiang Laboratory

Force spectroscopy experiments use mechanical force as a control factor to regulate the folding and unfolding process of proteins. Atomic force microscopy has been widely used to study the mechanical stability of proteins, and obtained unfolding forces and unfolding distance of different proteins, while recently, more low force folding and unfolding measurements were done by optical tweezers and magnetic tweezers. Due to the relatively small distortion of the free energy landscape, low force measurements give the free energy landscape information over bigger conformational space. In this review, we summarize the results of force spectroscopy experiments on different proteins. The unfolding distance obtained at high forces by atomic force microscopy are mostly smaller than 2 nm, while the unfolding distances at low forces distribute over a larger range: from a negative value to more than 6 nm. The sizes of the transition states at low force are ～4 nm for most compact two-state globular proteins,which indicates that this transition state might be the general free energy barrier separating the unfolded state and the theoretically predicated molten globule state. Up to now, only a limited number of proteins has been studied at low forces. We expect that more and more proteins with different conformations will be studied at low forces to reveal the general protein folding mechanism.

关键词： Protein folding Free energy landscape Force spectroscopy Molten globule state Transition state

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