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Microsystems & Nanoengineering 2017年第1期3卷 382-388页

作者： ziliang carter lin Allister F.McGuire Paul W.Burridge Elena Matsa Hsin-Ya Lou Joseph C.Wu Bianxiao Cui Department of Applied Physics Stanford UniversityStanfordCA 94305USA Department of Chemistry Stanford UniversityStanfordCA 94305USA Stanford Cardiovascular Institute Stanford UniversityStanfordCA 94305USA Department of Pharmacology and Center for Pharmacogenomics Northwestern UniversityChicagoIL 60611USA

The measurement of the electrophysiology of human pluripotent stem cell-derived cardiomyocytes is critical for their biomedical applications,from disease modeling to drug ***,a method that enables the high-throughput intracellular electrophysiology measurement of single cardiomyocytes in adherent culture is not *** address this area,we have fabricated vertical nanopillar electrodes that can record intracellular action potentials from up to 60 single beating *** access is achieved by highly localized electroporation,which allows for low impedance electrical access to the intracellular ***,we demonstrate that this method provides the accurate measurement of the shape and duration of intracellular action potentials,validated by patch clamp,and can facilitate cellular drug screening and disease modeling using human pluripotent stem *** study validates the use of nanopillar electrodes for myriad further applications of human pluripotent stem cell-derived cardiomyocytes such as cardiomyocyte maturation monitoring and electrophysiology-contractile force correlation.

关键词： cardiomyocytes drug screening electrophysiology human pluripotent stem cells multielectrode array

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欧拉-拉格朗日迭代固-液相变算法

化工学报 2024年第8期75卷 2763-2776页

作者：朱子良王爽姜宇昂林梅王秋旺西安交通大学热流科学与工程教育部重点实验室陕西西安710049 西安交通大学能源与动力工程学院陕西西安710049

在固-液相变过程中,外力可导致固体相变材料在液体相变材料中产生相对运动并严重影响相变流动传热。提出一种欧拉-拉格朗日迭代固-液相变算法,创新地将预测固体相对运动的拉格朗日迭代外置耦合于预测相变流动传热的欧拉迭代,能够稳定准... 详细信息

在固-液相变过程中,外力可导致固体相变材料在液体相变材料中产生相对运动并严重影响相变流动传热。提出一种欧拉-拉格朗日迭代固-液相变算法,创新地将预测固体相对运动的拉格朗日迭代外置耦合于预测相变流动传热的欧拉迭代,能够稳定准确地耦合计算固-液相变的流动传热和固体相变材料的相对运动。采用本算法研究了方腔内的接触熔化过程,探讨不同糊状区系数和重力加速度对本算法的影响规律。结果表明,本算法预测液相体积分数的平均误差为4.93%,固体相对运动预测数值振荡降低51.42%。对于石蜡材料,推荐使用的糊状区系数为1010。增大重力加速度会提高熔化速率并加快相对运动,但对整体趋势影响较小。研究结果可作为相变储能装置的设计参考。

关键词：相变传热算法数值模拟接触熔化相对运动内外迭代

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Frost heave and freezing processes of saturated rock with an open crack under different freezing conditions

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Frontiers of Structural and Civil Engineering 2020年第4期14卷 947-960页

作者： Zhitao Lv Caichu XIA Yuesong WANG ziliang lin Department of Geotechnical Engineering Tongji UniversityShanghai 200092China Key Laboratory of Geotechnical and Underground Engineering of Minisry of Education Tongji UniversinShanghai 200092China

Frost heave experiments on saturated sandstone and tuff with an open crack are conducted under uniform and unidirectional freezing *** heave of crack in sandstone with high permeability is more significant under uniform freezing condition than that under unidirectional freezing ***,frost heave of crack in tuff with low permeability is more significant under unidirectional freezing *** illustrate the reasons for this phenomenon,a numerical model on the freezing processes of saturated rock with an open crack considering the latent heat of pore water and water in crack is proposed and confirmed to be *** results show that a frozen shell that blocks the migration of water in crack to rock develops first in the outer part of the rock before the freezing of water in crack under unifonn freezing ***,the migration path of water in crack to the unfrozen rock under freezing front exists under unidirectional freezing *** freezing process and permeability of rock together determine the migration of water in crack and lead to the different frost heave modes of crack for various permeable rocks under different freezing *** frost heave modes of crack in rock with low or high permeability are similar under uniform freezing condition because water migration is blocked by a frozen shell and is irrelevant to rock *** high permeability rock,the frost heave of crack will be weakened due to water migration under unidirectional freezing condition;however,the frost heave of crack would be more significant for low permeability rock because water migration is blocked under unidirectional freezing ***,the freezing condition and rock permeability determine the frost heave of rock with crack together,and this should be concerned in cold regions engineering applications.

关键词： frost heave rock with crack freezing process freezing condition frost heave mode

NIR-II light activated photodynamic therapy with proteincapped gold nanoclusters

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Nano Research 2018年第10期11卷 5657-5669页

作者： Qian Chen Jiawen Chen Zhijuan Yang lin Zhang ziliang Dong Zhuang Liu Institute of Functional Nano & Soft Materials (FUNSOM) Jiangsu Key Laboratory for Carbon-based Functional Materials and Devices Soochow University Suzhou 215123 China Department of Obstetrics and Gynecology The First Affiliated Hospital of Soochow University Suzhou 215123 China

The use of near-infrared （NIR） light for photodynamic therapy （PDT） is a promising strategy to circumvent the limitations of current PDT, in which visible light with limited tissue penetration depth is usually used. In the present stud~ alkyl thiolated gold nanoclusters （AuNCs） were co-modified with human serum albumin （HSA） and catalase （CAT）, and then employed as a multifunctional, optical, theranostic nano-agent. In the AuNC@HSA/CAT system, the AuNCs were able to produce singlet oxygen under excitation by a 1,064-nm laser, which locates in the second NIR window （NIR-II）, and featured much lower tissue absorption and scattering, enabling NIR-II-triggered PDT. The HSA coating greatly improved the physiological stability of the nanoparticles, which showed efficient tumor retention after intravenous injection, as revealed by detecting the AuNC fluorescence. Moreover, the presence of CAT in the nanoparticles triggered decomposition of tumor endogenous H202 to generate oxygen, thereby enhancing the efficacy of PDT by relieving tumor hypoxia. Compared with conventional PDT using visible light, NIR-II-triggered PDT exhibits remarkably increased tissue penetration. Thus, we developed a new type of photosensitizing nano-agent that simultaneously enables in vivo fluorescence imaging, tumor hypoxia relief, and NIR-II light-induced in vivo PDT in the treatment of cancer.

关键词： gold nanoclusters the second near-infrared (NIR-II) light photodynamic therapy tumor hypoxia cancer theranostics

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铁死亡与肝脏缺血再灌注损伤关联的研究进展

中华肝胆外科杂志 2024年第4期30卷 304-307页

作者：景泽凡殷鹏张子亮陈阜东李昭凡林映雪崔翔王莉荔陈力解放军总医院第一医学中心全科医学科北京100853 上海市嘉定区江桥医院普通外科上海201812 解放军医学院北京100853 南开大学医学院天津300071 解放军总医院第四医学中心骨科北京100048

肝脏缺血再灌注损伤是肝切除和肝移植过程中常见的并发症,但其发生机制尚未明确,且缺乏有效的防治手段。铁死亡是一种新型的细胞死亡方式,涉及到铁依赖性脂质过氧化以及抗氧化系统的失衡,最终引起细胞死亡。铁死亡与肝缺血再灌注损伤在... 详细信息

肝脏缺血再灌注损伤是肝切除和肝移植过程中常见的并发症,但其发生机制尚未明确,且缺乏有效的防治手段。铁死亡是一种新型的细胞死亡方式,涉及到铁依赖性脂质过氧化以及抗氧化系统的失衡,最终引起细胞死亡。铁死亡与肝缺血再灌注损伤在机制特点上似乎有着共通之处,针对其相关性进一步研究可能为肝缺血再灌注损伤的预防和治疗提供策略。本文主要就铁死亡与肝缺血再灌注损伤的关联进行综述。

关键词：肝细胞再灌注损伤铁死亡铁代谢氧化应激

PIKA as an Adjuvant Enhances Specific Humoral and Cellular Immune Responses Following the Vaccination of Mice with HBsAg plus PIKA

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Cellular & Molecular Immunology 2007年第2期4卷 113-120页

作者： Erxia Shen Li Li Lietao Li Lianqiang Feng lin Lu ziliang Yao Haixiang lin Changyou Wu Department of Immunology Zhongshan School of Medicine Sun Yat-sen University Guangzhou Guangdong 510080 China State Ministry of Education Key Laboratory of Tropical Diseases Control Research Sun Yat-sen University Guangzhou Guangdong 510080 China NewBiomed PIKA （Guangzhou） Ltd. Guangzhou International Business Incubator Block-A A1210 Unit Guangzhou Science Park Guangzhou Guangdong 510663 China Guangdong Medical Laboratory Animal Center 119 Boyan Road Huangqi Distric Nanhai Foshan Guangdong 528248 China

An adjuvant is usually used to enhance the immune response induced by vaccines. The choice of adjuvant or immune enhancer determines the effectiveness of the immune response. Currently, aluminium （Alum, a generic term for salts of aluminium） is the only FDA-approved adjuvant. Alum predominantly induces the differentiation of Th2 cells and thus mediates an antibody immune response. Therefore, there is an urgent need for new adjuvants that enhance not only humoral but also cellular immune responses. In the present study, we demonstrates that PIKA （a stabilized dsRNA） as an adjuvant directly induces the activation and the proliferation of both B and NK cells in vitro. Injection of PIKA into mice results in the production of cytokines in vivo. In addition, the study demonstrates that PIKA promotes the maturation of bone marrow-derived dendritic cells （BMDCs） including up-regulation of the co-stimulatory molecules CD80, CD86 and CD40, and the induction of cytokines such as IL-12p70, IL-12p40 and IL-6. Importantly, after immunization of mice with HBsAg plus PIKA, the presence of PIKA enhances the titers of HBsAg-specific IgG and HBsAg-specific IFN-γ production. These results demonstrate that PIKA as an adjuvant can promote both humoral and cellular immune responses. These might have an implication in applying PIKA as an adjuvant to be used in the design and development of both therapeutic and preventive vaccines, and used in the clinical study.

关键词： PIKA adjuvant Toll like receptor HBsAg vaccine dendritic cell

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交联型自修复水性聚氨酯的合成与性能

工程塑料应用 2024年第8期52卷 39-45页

作者：党海春武美君边祥成林先鸿梁晋帆李杰赵剑波张子梁太原工业学院材料工程系太原030008

水性聚氨酯膜容易被划伤,利用双硫键引发的动态交换反应可赋予水性聚氨酯自修复能力。但动态共价键键合强度较弱,导致水性聚氨酯的自修复和力学性能难以兼得。采用聚环氧丙烷醚三醇和含双硫键的2-羟乙基二硫化物,在水性聚氨酯分子中同... 详细信息

水性聚氨酯膜容易被划伤,利用双硫键引发的动态交换反应可赋予水性聚氨酯自修复能力。但动态共价键键合强度较弱,导致水性聚氨酯的自修复和力学性能难以兼得。采用聚环氧丙烷醚三醇和含双硫键的2-羟乙基二硫化物,在水性聚氨酯分子中同时引入双硫键和支化结构,制得高强度、交联型的自修复水性聚氨酯。以异氟尔酮二异氰酸酯(IPDI)、聚四氢呋喃醚二醇、聚环氧丙烷醚三醇、2-双(羟甲基)丙酸(DMPA)以及2-羟乙基二硫化物(HEDS)等为主要原料,经预聚物法合成不同DMPA/HEDS物质的量比的IPDI型交联型自修复水性聚氨酯,研究DMPA与HEDS物质的量比对水性聚氨酯乳液稳定性、水性聚氨酯胶膜力学性能和自修复性能的影响。采用傅里叶变换红外光谱(FTIR)仪和拉曼光谱对其进行结构表征,验证了含双硫键的自修复水性聚氨酯的成功合成。双硫键和支化结构的同时引入显著提高了水性聚氨酯胶膜的拉伸强度和自修复性能,当DMPA与HEDS物质的量比为1∶1时,拉伸强度从不含双硫键聚氨酯胶膜的13.1 MPa提高到24.2 MPa,自修复后强度达19.4 MPa,自修复率达80.1%。

关键词：水性聚氨酯双硫键交联自修复胶膜

紫云英与水稻秸秆联合还田配合氮肥减施对水稻产量及氮素营养平衡的影响

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江苏农业学报 2024年第6期40卷 1012-1019页

作者：杜光辉张琳丁丽聂良鹏史鹏飞吕玉虎张丽霞潘兹亮孙全东信阳市农业科学院河南信阳464001 信阳市农业农村局河南信阳464001

紫云英与水稻秸秆联合还田是减肥增效的重要举措,通过试验研究紫云英与水稻秸秆联合还田配合氮肥减施对水稻产量及氮素营养平衡的影响,以期为豫南稻区合理利用紫云英与水稻秸秆提供理论依据。氮肥设计按照常规施用量的60%氮(N0.6)、100%... 详细信息

紫云英与水稻秸秆联合还田是减肥增效的重要举措,通过试验研究紫云英与水稻秸秆联合还田配合氮肥减施对水稻产量及氮素营养平衡的影响,以期为豫南稻区合理利用紫云英与水稻秸秆提供理论依据。氮肥设计按照常规施用量的60%氮(N0.6)、100%氮(N)2个水平;水稻秸秆采取留高茬还田(St)、不还田2种方式;绿肥采取种植并翻压紫云英(Mv, 22 500 kg/hm^(2))、冬闲2种模式;以冬闲和水稻秸秆不还田不施肥作空白对照(CK),共10个处理,研究紫云英与水稻秸秆联合还田配合氮肥减施对水稻产量、水稻地上部氮积累量、土壤氮含量、氮肥利用率及土壤氮素表观盈亏量的影响。结果表明,紫云英与水稻秸秆联合还田配施60%氮肥(MvStN0.6)的水稻产量较冬闲田水稻秸秆不还田100%氮肥(N)、紫云英或水稻秸秆还田配施等量氮肥处理(MvN0.6、StN0.6)分别显著增加4.77%、6.49%、23.48%。紫云英还田处理的水稻地上部氮积累量均显著高于其他等量氮肥处理。与冬闲田水稻秸秆不还田100%氮肥处理相比,紫云英还田配施100%氮肥处理显著增加水稻土碱解氮含量,减氮处理中仅MvStN0.6处理显著增加了土壤全氮含量。紫云英与水稻秸秆联合还田配施氮肥处理较冬闲田水稻秸秆不还田配施等量氮肥的氮肥表观利用率均显著提高,MvStN0.6处理较N0.6处理显著增加了氮肥实际利用率。不同处理土壤氮素表观盈亏量差异较大,为-67~69 kg/hm^(2)。综合来看,紫云英与水稻秸秆联合还田配施60%氮肥可有效增加水稻地上部氮的积累,提高水稻的收获指数和氮肥实际利用率,进而增加水稻产量,降低氮肥面源污染。

关键词：紫云英水稻秸秆产量氮积累氮素表观盈亏量

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不同磨粉工艺对大米粉及鲜湿米粉品质的影响

食品工业 2024年第3期45卷 30-34页

作者：梁钦梅程子良王福成林梢姚镇江林莹广西大学轻工与食品工程学院南宁530004 柳州市质量检验检测研究中心柳州545005 广西螺霸王食品科技有限公司柳州545005

文章为大米粉作为商品化产品提供理论依据,为工业化生产鲜湿米粉提供参考。以干磨、半干磨、湿磨三类工艺制作大米粉,测量大米粉理化指标、鲜湿米粉品质指标,并对数据进行PCA和PLS-VIP分析。结果表明,不同米水比湿法磨粉粒径(D_(50) 34.... 详细信息

文章为大米粉作为商品化产品提供理论依据,为工业化生产鲜湿米粉提供参考。以干磨、半干磨、湿磨三类工艺制作大米粉,测量大米粉理化指标、鲜湿米粉品质指标,并对数据进行PCA和PLS-VIP分析。结果表明,不同米水比湿法磨粉粒径(D_(50) 34.90~36.94μm)和破损淀粉含量(0.68%~1.09%)没有显著区别,其具有较低的衰减值(548~722 cP)和回生值(153~1 762 cP),制作的米制品抗回生性好。鲜湿米粉方面,用湿磨粉和调节水分至25%和30%的半干法磨粉制作的鲜湿米粉品质优良。PLSR-VIP分析表明粒度和破损淀粉会影响鲜湿米粉品质。

关键词：磨粉工艺大米粉鲜湿米粉理化性质品质比较

A Comparative Study of the Electrodes Gels’Electrical Properties in the Measurement Issues of Intrabody Communication

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Journal of Beijing Institute of Technology 2022年第1期31卷 71-80页

作者： Fengjie lin ziliang Wei Jiejie Yang Yangrong Wen Yueming Gao Sio Hang Pun Mang I Vai Min Du College of Physics and Information Engineering Key Laboratory of Medical Instrumentation&Pharmaceutical Technology of Fujian ProvinceFuzhou UniversityFuzhou 350108China State Key Laboratory of Analog and Mixed-Signal VLSI University of MacaoTaipaMO 999078China

Intrabody communication(IBC)technology is becoming progressively more standard-ized due to its low power consumption and high security features incorporated into the third phys-ical layer of the IEEE 802.15.6 *** then,there are still many challenges in normalizing the measurement issues of IBC.A major concern that should not be overlooked is the electrodes in the IBC,especially the popular use of gel *** the channel measurements,gel electrodes are commonly employed to improve the signal-to-noise ratio and prevent electrodes from falling *** this paper,a comparative study of the electrical properties of gel was investigated during the measurement of human channel characteristics and to clarify the differences of ***,the basis of electrostatic field pole plate measurements and electromagnetic theory were introduced to interpretate how the relative permittivity and conductivity of different gels will influence the meas-urement *** the in vivo experiments with different gel or dry electrodes were performed to compare the differences induced by the *** results indicate that the influence of the gel on the human channel measurement is mainly concentrated below 400 kHz(the attenuation is re-duced by 16.7 dB on average),and the stability of the permittivity and conductivity of the gel has a direct impact on the stability of its measurement of the human *** result may provide a meaningful reference for the standardization of electrode usage in IBC.

关键词： intrabody communication channel characteristic measurement gel electrodes permit-tivity conductivity