检索结果-南通市图书馆

Unraveling structure and performance of protein a ligands at liquid–solid interfaces: A multi-techniques analysis

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemical Engineering 2023年第2期54卷 232-239页

作者： Yi Shen Xinshuang chu Qinghong Shi Department of Biochemical Engineering School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300350China Key Laboratory of Systems Bioengineering and Frontiers Science Center for Synthetic Biology(Ministry of Education) Tianjin UniversityTianjin 300350China

Oriented ligand immobilization is one of the most effective strategies used in the design and construction of a high-capacity protein A chromatography. In this work, cysteine was introduced as anchoring sites by substituting a specific residue on Helix Ⅰ, Ⅱ, and at C-terminus of antibody binding domain Z from protein A, respectively, to investigate structural evolution and binding behavior of protein A ligands at liquid-solid interfaces. Among the three affinity dextran-coated Fe_(3)O_(4) magnetic nanoparticles(Fe_(3)O_(4)@Dx MNPs), affinity MNPs with the immobilized ligand via N11C on Helix Ⅰ(Fe_(3)O_(4)@Dx-Z_(1) MNPs) had the highest helical content, and MNPs with the immobilized ligand via g29C on Helix Ⅱ(Fe_(3)O_(4)@Dx-Z_(2) MNPs) had the lowest helical content at the same pHs. It was attributed to less electrostatic attraction of ligand to negatively charged surface on Fe_(3)O_(4)@Dx-Z_(1) MNPs because of less positive charged residues on Helix Ⅰ(K6) than Helix Ⅱ(R27/K35). Among the three affinity MNPs, moreover, the highest affinity to immunoglobulin g(Igg) binding was observed on Fe_(3)O_(4)@Dx-Z_(1) MNPs in isothermal titration calorimetry measurement, further validating greater structural integrity of the ligand on Fe_(3)O_(4)@Dx-Z_(1) MNPs. Finally,the study of Igg binding on MNPs and 96-well plates showed that anchoring sites for ligand immobilization had distinct influences on Igg binding and Igg-mediated antigen binding. This work illustrated that anchoring sites of the ligands had a striking significance for the molecular structure of the ligand at liquid-solid interfaces and raised an important implication for the design and optimization of protein A chromatography and protein A-based immunoassay analysis.

关键词： Adsorption Interface Thermodynamics Protein A ligand Immobilization Molecular structure

Fe-Co/ZIF-8@SLC-0111-HA复合纳米平台加强肿瘤化学动力学的可行性

在线全文

学校读者我要写书评

暂无评论

中国组织工程研究 2024年第29期28卷 4612-4619页

作者：王振鑫周鹏褚福超张大振袁峰徐州医科大学第一临床医学院江苏省徐州市221006 徐州医科大学附属淮安医院淮安市第二人民医院骨科江苏省淮安市223002 徐州医科大学附属医院骨科江苏省徐州市221006

背景:常用金属离子的低催化活性与缺乏靶向性等问题严重限制了化学动力学疗法在肿瘤治疗中的应用。另外,虽然通过对复合纳米平台进行表面功能化来赋予其靶向肿瘤的功能,但是肿瘤细胞内酸性不足也严重削弱了化学动力学疗法的疗效。目的:... 详细信息

背景:常用金属离子的低催化活性与缺乏靶向性等问题严重限制了化学动力学疗法在肿瘤治疗中的应用。另外,虽然通过对复合纳米平台进行表面功能化来赋予其靶向肿瘤的功能,但是肿瘤细胞内酸性不足也严重削弱了化学动力学疗法的疗效。目的:制备新型复合纳米平台,评估其在细胞水平上增强化学动力学疗法的可行性。方法:通过离子交换反应和自组装作用合成了掺杂二价铁离子和二价钴离子的载SLC-0111(一种碳酸酐酶9抑制剂)沸石咪唑骨架-8(Fe-Co/ZIF-8@SLC-0111),并在表面加载透明质酸,得到目标纳米颗粒Fe-Co/ZIF-8@SLC-0111-HA(记为FC-S),同时合成不载SLC-0111的纳米颗粒Fe-Co/ZIF-8-HA(记为FC)。测试FC-S的粒径、Zeta电位、表面形貌、体外活性氧产生、消耗谷胱甘肽的能力。分别以人骨肉瘤细胞Mg-63和小鼠成纤维细胞L929为实验对象,采用CCK-8法检测FC-S的细胞毒性。以人骨肉瘤细胞Mg-63为实验对象,检测FC-S的细胞内化;在加入H_(2)O_(2)的情况下,FC-S、FC对细胞内pH值、碳酸酐酶9蛋白表达、细胞活性与凋亡、细胞内活性氧与谷胱甘肽含量、细胞线粒体膜电位的影响。结果与结论:①FC-S具有菱形十二面体结构,尺寸均匀,分散良好,平均粒径为323 nm,Zeta电位约为-11.1 mV,体外可产生活性氧并消耗谷胱甘肽。②FC-S以时间依赖的方式在Mg-63细胞内累积,并且能成功从溶酶体中逃逸。当FC-S质量浓度≤20μg/mL时对Mg-63细胞与L929细胞无明显的细胞毒性,后续实验选择20μg/mL FC-S作用于Mg-63细胞。③与FC组比较,FC-S组Mg-63细胞内碳酸酐酶9蛋白表达降低(P<0.01)、细胞内酸性环境增强、细胞内活性氧含量增加(P<0.001)、细胞线粒体损伤加重、死细胞数量增加、细胞凋亡率升高(P<0.001)。④结果表明,FC-S复合纳米平台能够有效改善肿瘤细胞内弱酸性微环境、提升胞内活性氧产生水平,增强化学动力学疗法的疗效。

关键词：纳米平台化学动力学疗法碳酸酐酶9 碳酸酐酶9抑制剂活性氧细胞凋亡

在线全文

学校读者我要写书评

暂无评论

不同发酵条件对玉米秸秆发酵品质的影响

饲料研究 2024年第11期47卷 126-131页

作者：楚云开李建涛韩淑敏沈阳农业大学动物科学与医学学院辽宁沈阳110161 辽宁九州生物科技有限公司辽宁铁岭112609

试验旨在探究不同的发酵条件对玉米秸秆的发酵效果。在相同的环境条件下对3组秸秆(试验组1:玉米秸秆10 kg+酶制剂50 g+酵母菌10 g;试验组2:玉米秸秆6 kg+精料4 kg+酶制剂50 g+酵母菌10 g;试验组3:玉米秸秆10 kg+石灰水+酶制剂50 g+酵母... 详细信息

试验旨在探究不同的发酵条件对玉米秸秆的发酵效果。在相同的环境条件下对3组秸秆(试验组1:玉米秸秆10 kg+酶制剂50 g+酵母菌10 g;试验组2:玉米秸秆6 kg+精料4 kg+酶制剂50 g+酵母菌10 g;试验组3:玉米秸秆10 kg+石灰水+酶制剂50 g+酵母菌10 g)进行密闭堆积发酵处理,并在发酵1、3、5、7 d检测水分、中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)、粗蛋白(CP)、粗脂肪(EE)、pH值、还原糖、酵母菌、感官指标等,探讨不同试验组的发酵效果并筛选出最佳发酵条件。结果显示:发酵7 d时,试验组1的NDF含量显著低于0、1、5 d(Pg/t)+活性酵母菌(1 kg/t)发酵7 d的效果较好。

关键词：发酵玉米秸秆复合酶活性酵母菌

维普期刊数据库博看期刊

盐酸氨溴索配合支气管镜肺泡灌洗治疗对重症肺炎伴肺不张患者症状改善时间、炎症反应的影响

在线全文

学校读者我要写书评

暂无评论

反射疗法与康复医学 2024年第7期5卷 85-88页

作者：朱善香褚波青岛大学附属泰安市中心医院碧霞湖院区重症康复科青岛大学附属泰安市中心医院急诊内科

目的探讨盐酸氨溴索配合支气管镜肺泡灌洗在重症肺炎伴肺不张患者中的应用效果。方法选取2023年1—9月我院收治的60例重症肺炎伴肺不张患者为研究对象，按随机数字表法将其分为对照组与观察组，各30例。对照组采用支气管镜肺泡灌洗治... 详细信息

目的探讨盐酸氨溴索配合支气管镜肺泡灌洗在重症肺炎伴肺不张患者中的应用效果。方法选取2023年1—9月我院收治的60例重症肺炎伴肺不张患者为研究对象，按随机数字表法将其分为对照组与观察组，各30例。对照组采用支气管镜肺泡灌洗治疗，观察组在此基础上加用盐酸氨溴索治疗，持续治疗2周。比较两组的炎症反应、症状改善时间、肺功能指标、血气指标差异。结果治疗后，观察组白细胞介素-6、C反应蛋白、降钙素原水平分别为（47.36±4.33）pg/mL、（7.35±1.02）mg/L、（0.45±0.11）μg/L，均低于对照组的（52.43±4.96）pg/mL、（9.46±1.13）mg/L、（0.71±0.13）μg/L，组间差异有统计学意义（Pg（1 mmHg=0.133 kPa），高于对照组的（84.57±6.22）mmHg，二氧化碳分压为（38.47±3.43）mmHg，低于对照组的（42.46±4.02）mmHg，组间差异有统计学意义（P<0.05）。结论盐酸氨溴索联合支气管镜肺泡灌洗可加快重症肺炎伴肺不张患者的炎症消退，减轻肺功能障碍，纠正血气异常，促进疾病症状消失。

关键词：重症肺炎肺不张盐酸氨溴索支气管镜肺泡灌洗炎症反应

Seismic fragility curves for structures using non-parametric representations

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Structural and Civil Engineering 2017年第2期11卷 169-186页

作者： chu MAI Katerina KONAKLI Bruno SUDRET Risk Safety & Uncertainty Quantification Institute of Structural Engineering ETH Ziirich Ziirich CH-8093 Switzerland

Fragility curves are commonly used in civil engineering to assess the vulnerability of structures to earthquakes. The probability of failure associated with a prescribed criterion （e.g., the maximal inter-storey drift of a building exceeding a certain threshold） is represented as a function of the intensity of the earthquake ground motion （e.g., peak ground acceleration or spectral acceleration）. The classical approach relies on assuming a lognormal shape of the fragility curves; it is thus parametric. In this paper, we introduce two non-parametric approaches to establish the fragility curves without employing the above assumption, namely binned Monte Carlo simulation and kernel density estimation. As an illustration, we compute the fragility curves for a three-storey steel frame using a large number of synthetic ground motions. The curves obtained with the non-parametric approaches are compared with respective curves based on the lognormal assumption. A similar comparison is presented for a case when a limited number of recorded ground motions is available. It is found that the accuracy of the lognormal curves depends on the ground motion intensity measure, the failure criterion and most importantly, on the employed method for estimating the parameters of the lognormal shape.

关键词： earthquake engineering fragility curves lognormal assumption non-parametric approach kernel density estimation epistemic uncertainty

Carbonaceous and nitrogenous disinfection by-product formation in the surface and ground water treatment plants using Yellow River as water source

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2012年第7期24卷 1204-1209页

作者： Yukun Hou Wenhai chu Meng Ma College of Environmental Science and Engineering Tongji University Shanghai 200092 China Zhengzhou Water Supply Corporation Zhengzhou 450013 China

This work investigated the formation of carbonaceous and nitrogenous disinfection by-products （C-DBPs, N-DBPs） upon chlorination of water samples collected from a surface water and a ground water treatment plant （SWTP and gWTP） where the conventional treatment processes, i.e., coagulation, sedimentation, and filtration were employed. Twenty DBPs, including four trihalomethanes, nine haloacetic acids, seven N-DBPs （dichloroacetamide, trichloroacetamide, dichloroacetonitrile, trichloroacetonitrile, bromochloroace- tonitrile, dibromoacetonitrile and trichloronitromethane）, and eight volatile chlorinated compounds （dichloromethane （DCM）, 1,2-dichloroethane, tetrachloroethylene, chlorobenzene, 1,2-dichlorobenzene, 1,4-dichlorobenzene, 1,2,3-trichlorobenzene and 1,2,4- trichlorobenzene） were detected in the two WTPs. The concentrations of these contaminants were all below their corresponding maximum contamination levels （MCLs） regulated by the Standards for Drinking Water Quality of China （gB5749-2006） except for DCM （17.1 ~tg/L detected vs. 20 μg/L MCL）. The SWTP had much higher concentrations of DBPs detected in the treated water as well as the DBP formation potentials tested in the filtered water than the gWTP, probably because more precursors （e.g., dissolved organic carbon, dissolved organic nitrogen） were present in the water source of the SWTE

关键词： carbonaceous disinfection by-products nitrogenous disinfection by-products formation potential free chlorine watertreatment plant

Optimization on bicriterion policies for M/g/1 system with second optional service

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2008年第10期9卷 1437-1445页

作者： Jau-chuan KE Yunn-kuang chu Department of Applied Statistics National Taichung Institute of Technology Taichung 404 Taiwan China

We compare the optimal operating cost of the two bicriterion policies, and , for an M/g/1 queueing system with second optional service, in which the length of the vacation period is randomly controlled either by the number of arrivals during the idle period or by a timer. After all the customers are served in the queue exhaustively, the server immediately takes a vacation and may operate policy or policy. For the two bicriterion policies, the total average cost function per unit time is developed to search the optimal stationary operating policies at a minimum cost. Based upon the optimal cost the explicit forms for joint optimum threshold values of (p,T) and (p,N) are obtained.

关键词： Average operating cost Bicriterion policy Optimization comparisons Optional service Optimal threshold values

基于“三因制宜”理念的中医辨证护理在全髋关节置换术患者中的应用效果分析

在线全文

学校读者我要写书评

暂无评论

西部中医药 2024年第3期37卷 126-130页

作者：褚俊链郭容弟河北省沧州中西医结合医院河北沧州061000 沧州市和平医院河北沧州061001

目的:观察“三因制宜”理念的中医辨证护理在全髋关节置换术患者中的应用效果。方法:将88例全髋关节置换术(total hip arthroplasty,THA)患者按随机数字表法分为对照组和三因组各44例。对照组给予西医常规护理,三因组在对照组的基础上... 详细信息

目的:观察“三因制宜”理念的中医辨证护理在全髋关节置换术患者中的应用效果。方法:将88例全髋关节置换术(total hip arthroplasty,THA)患者按随机数字表法分为对照组和三因组各44例。对照组给予西医常规护理,三因组在对照组的基础上给予“三因制宜”理念的中医辨证护理。观察两组患者术前及术后6天视觉模拟量表(visual analogue scale,VAS)和汉密尔顿焦虑量表(hamilton anxiety scale,HAMA)评分、术后7天炎症指标[白细胞介素6(interleukin-6,IL-6)、肿瘤坏死因子α(tumor necrosis factorα,TNF-α)]、术后5个月Harris髋关节评分、并发症及护理满意度。结果:术后6天两组患者VAS和HAMA评分均低于术前(P0.05);三因组患者并发症总发生率为2.27%(1/44),低于对照组的13.64%(6/44)(P<0.05);三因组患者总满意度为95.45%(42/44),高于对照组的79.55%(35/44)(P<0.05)。结论:基于“三因制宜”理念的中医辨证护理可有效缓解THA患者疼痛,抑制炎症反应、改善髋关节功能,降低术后并发症,提高护理满意度。

关键词：全髋关节置换术三因制宜中医辨证护理效果分析

煤基针状焦制备超级电容器电极材料及其电化学性能

在线全文

学校读者我要写书评

暂无评论

黑龙江科技大学学报 2024年第1期34卷 40-46页

作者：熊楚安王楠封迈黑龙江科技大学环境与化工学院哈尔滨150022

为了探究煤基针状焦的电化学性能,利用中温煤焦油沥青针状焦生焦和KOH活化剂,通过高温热处理法,制备了用于超级电容器的多孔碳材料,并进行了SEM、XRD、红外光谱表征和氮气吸附-脱附测试,对碳材料电极进行了循环伏安测试、电化学阻抗谱... 详细信息

为了探究煤基针状焦的电化学性能,利用中温煤焦油沥青针状焦生焦和KOH活化剂,通过高温热处理法,制备了用于超级电容器的多孔碳材料,并进行了SEM、XRD、红外光谱表征和氮气吸附-脱附测试,对碳材料电极进行了循环伏安测试、电化学阻抗谱、恒电流充放电等电化学性能测试,研究了KOH添加量对碳材料电极的电化学性能影响。结果表明:当碱碳比为2∶1时,电流密度为0.1 A/g时,其质量比电容达到222.83 F/g;电流密度从0.5 A/g增至10 A/g时,电容保持率能够达到93.2%;5 A/g循环10 000圈之后,比电容能够保持100%。

关键词：煤基针状焦电极材料活化电化学性能