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Two intestinal microbiota-derived metabolites, deoxycholic acid and butyrate, synergize to enhance host defense peptide synthesis and alleviate necrotic enteritis

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Journal of Animal Science and Biotechnology 2024年第3期15卷 1158-1172页

作者： Dohyung M.Kim Jing liu Melanie A.Whitmore Isabel Tobin zijun Zhao Guolong Zhang Department of Animal and Food Sciences Oklahoma State UniversityStillwaterOKUSA

Background Necrotic enteritis(NE)is a major enteric disease in poultry,yet effective mitigation strategies remain elusive.Deoxycholic acid(DCA)and butyrate,two major metabolites derived from the intestinal microbiota,have independently been shown to induce host defense peptide(HDP)synthesis.However,the potential synergy between these two compounds remains unexplored.Methods To investigate the possible synergistic effect between DCA and butyrate in regulating HDP synthesis and barrier function,we treated chicken HD11 macrophage cells and jejunal explants with DCA and sodium butyrate(NaB),either individually or in combination,for 24 h.Subsequently,we performed RNA isolation and reverse transcrip-tion-quantitative PCR to analyze HDP genes as well as the major genes associated with barrier function.To further determine the synergy between DCA and NaB in enhancing NE resistance,we conducted two independent trials with Cobb broiler chicks.In each trial,the diet was supplemented with DCA or NaB on the day-of-hatch,followed by NE induction through sequential challenges with Eimeria maxima and Clostridium perfringens on d 10 and 14,respectively.We recorded animal mortality after infection and assessed intestinal lesions on d 17.The impact of DCA and NaB on the microbiota in the ileum and cecum was evaluated through bacterial 16S rRNA gene sequencing.Results We found that the combination of DCA and NaB synergistically induced multiple HDP genes in both chicken HD11 cells and jejunal explants.Additionally,the gene for claudin-1,a major tight junction protein,also exhibited synergistic induction in response to DCA and NaB.Furthermore,dietary supplementation with a combination of 0.75 g/kg DCA and 1 g/kg NaB led to a significant improvement in animal survival and a reduction in intestinal lesions compared to either compound alone in a chicken model of NE.Notably,the cecal microbiota of NE-infected chickens showed a marked decrease in SCFA-producing bacteria such as Bacteroides,Faecalibacterium,and Cuneatibacter,with lactobaci

关键词： Bile acids Host defense peptides Metabolites Microbiome Necrotic enteritis Short-chain fatty acids

Encouraging Solution to the Problem of Synthesizing Protein α-Thioester

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Chinese Journal of Chemistry 2024年第10期42卷 1114-1120页

作者： Xinliang liu zijun Gao Jie Zhao Farong Ye Ping Huang Ping Wang Center for Chemical Glycobiology Shanghai Key Laboratory for Molecular Engineering of Chiral DrugsFrontiers Science Center for Transformative MoleculesSchool of Chemistry and Chemical EngineeringZhangjiang Institute for Advanced StudyShanghai JiaoTongUniversityShanghai 200240China Shenzhen Research Institute of Shanghai Jiao Tong University ShenzhenGuangdong 518057China

Expressed protein ligation(EPL)provides a powerful tool to access large-size proteins with precise structures.Existing methods for constructing the critical protein thioester for EPL have predominantly relied on the recombinant intein fusion expressed in Escherichia coli(E.coli).Despite its powerful applications,the expression of thioester derived from eukaryotic protein in E.coli inherently suffers from its limited solubility,the inactivity of intein,premature hydrolysis and low yields.To overcome these obstacles,we present herein the facile one-flask synthesis of inaccessible proteinα-thioester via a SUMO-protein-intein(SPI)sandwich model.The utility of SUMO enhances the protein fusion yield and solubility,prevents premature hydrolysis and simplifies the purification process.The inaccessible protein thioester with internal Cys residues can be readily produced and is compatible with the EPL-desulfurization protocol used to prepare complex proteins,which is otherwise difficult to obtain using traditional methods.Its utility has been highlighted through the synthesis of human granulocyte colony-stimulating factor(G-CSF).

关键词： Desulfurization Glycoproteins Glycopeptides Protein expression Peptide thioester

Two ABCI family transporters,OsABCI15 and OsABCI16,are involved in grain-filling in rice

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Journal of Genetics and Genomics 2024年第5期51卷 492-506页

作者： Bin Ma Xiubiao Cao Xiaoyuan Li Zhong Bian Qi-Qi Zhang zijun Fang Jiyun liu Qun Li Qiaoquan liu Lin Zhang Zuhua He Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology Agricultural College of Yangzhou UniversityYangzhouJiangsu 225009China Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Jiangsu Key Laboratory of Crop Genetics and PhysiologyYangzhou UniversityYangzhouJiangsu 225009China National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology&EcologyChinese Academy of SciencesShanghai 200032China Institute of Biotechnology Hangzhou Academy of Agricultural SciencesHangzhouZhejiang 310024China Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education Yangzhou UniversityYangzhouJiangsu 225009China

Seed development is critical for plant reproduction and crop yield,with panicle seed-setting rate,grain-filling,and grain weight being key seed characteristics for yield improvement.However,few genes are known to regulate grain filling.Here,we identify two adenosine triphosphate(ATP)-binding cassette(ABC)I-type transporter genes,OsABCI15 and OsABCI16,involved in rice grain-filling.Both genes are highly expressed in developing seeds,and their proteins are localized to the plasma membrane and cytosol.Interestingly,knockout of OsABCI15 and OsABCI16 results in a significant reduction in seed-setting rate,caused predominantly by the severe empty pericarp phenotype,which differs from the previously reported low seed-setting phenotype resulting from failed pollination.Further analysis indicates that OsABCI15 and OsABCI16 participate in ion homeostasis and likely export ions between filial tissues and maternal tissues during grain filling.Importantly,overexpression of OsABCI15 and OsABCI16 enhances the seed-setting rate and grain yield in transgenic plants and decreases ion accumulation in brown rice.Moreover,the OsABCI15/16 orthologues in maize exhibit a similar role in kernel development,as demonstrated by their disruption in transgenic maize.Therefore,ourﬁndings reveal the important roles of two ABC transporters in cereal grain filling,highlighting their value in crop yield improvement.

关键词： ABC transporters Rice Grain filling Ion homeostasis Grain yield

A Novel Braking Control Strategy for Hybrid Electric Buses Based on Vehicle Mass and Road Slope Estimation

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Chinese Journal of Mechanical Engineering 2022年第6期35卷 340-350页

作者： zijun liu Shuo Cheng Jinzhao liu Qiong Wu Liang Li Huawei Liang State Key Laboratory of Automotive Safety and Energy Tsinghua UniversityBeijing 100084China Beijing Forestry University Beijing 100091China University of Science and Technology Hefei 230026China Institute of Applied Technology Hefei Institutes of Physical ScienceChinese Academy of SciencesHefei 230088China

Proper braking force distribution strategies can improve both stability and economy performance of hybrid electric vehicles,which is prominently proved by many studies.To achieve better dynamic stable performance and higher energy recovery efficiency,an effective braking control strategy for hybrid electric buses(HEB)based on vehicle mass and road slope estimation is proposed in this paper.Firstly,the road slope and the vehicle mass are estimated by a hybrid algorithm of extended Kalman filter(EKF)and recursive least square(RLS).Secondly,the total braking torque of HEB is calculated by the sliding mode controller(SMC),which uses the information of brake intensity,whole vehicle mass,and road slope.Finally,comprehensively considering driver’s braking intention and regulations of the Economic Commission for Europe(ECE),the optimal proportional relationship between regenerative braking and pneumatic braking is obtained.Furthermore,related simulations and experiments are carried out on the hardware-in-the-loop test bench.Results show that the proposed strategy can effectively improve the braking performance and increase the recovered energy through precise control of the braking torque.

关键词： Hybrid electric bus Vehicle mass estimation Road slope estimation Braking control strategy Regenerative braking

Secure control for discrete-time hidden Markov jump systems subject to replay attacks via output feedback

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Journal of Control and Decision 2023年第4期10卷 584-595页

作者： Lei Su Shinian Fang zijun liu Hao Shen Tian Fang Anhui Province Key Laboratory of Special Heavy Load Robot Anhui University of TechnologyMa’anshanPeople’s Republic of China School of Electrical and Information Engineering Anhui University of TechnologyMa’anshanPeople’s Republic of China MCC Huatian Engineering and Technology Corporation Ma’anshanPeople’s Republic of China

This paper investigates the static output feedback secure control problem for discrete-time hidden Markov jump systems against replay attacks. The main purpose is to realise that closed-loopsystems are stochastically stable with or without replay attacks. Firstly, the tampered sensorsunder replay attacks can be identified via the proposed detection method. Then, an asynchronousstatic output feedback controller is designed, which can eliminate the negative impactcaused by replay attacks in view of the detection results. Based on the linear matrix inequalitytechnique, some sufficient conditions which ensure the closed-loop systems are stochasticallystable and meet a given H∞ performance are established. Finally, a numerical example and apractical example are given to verify the effectiveness and superiority of the proposed method.

关键词： Hidden Markov jump systems static output feedback control replay attacks H∞performance

Coxsackievirus B3 HFMD animal models in Syrian hamster and rhesus monkey

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Virologica Sinica 2024年第2期39卷 290-300页

作者： Suqin Duan Wei Zhang Yongjie Li Yanyan Li Yuan Zhao Weihua Jin Quan liu Mingxue Li Wenting Sun Lixiong Chen Hongjie Xu Jie Tang Jinghan Hou zijun Deng Fengmei Yang Shaohui Ma Zhanlong He Institute of Medical Biology Chinese Academy of Medical Sciences Medical Primate Research Center Peking Union Medical CollegeYunnan Key Laboratory of Vaccine Research Development on Severe Infectious DiseaseKunming650118China

Coxsackievirus B3(CVB3)is the pathogen causing hand,foot and mouth disease(HFMD),which manifests across a spectrum of clinical severity from mild to severe.However,CVB3-infected mouse models mainly demonstrate viral myocarditis and pancreatitis,failing to replicate human HFMD symptoms.Although several enteroviruses have been evaluated in Syrian hamsters and rhesus monkeys,there is no comprehensive data on CVB3.In this study,we have first tested the susceptibility of Syrian hamsters to CVB3 infection via different routes.The results showed that Syrian hamsters were successfully infected with CVB3 by intraperitoneal injection or nasal drip,leading to nasopharyngeal colonization,acute severe pathological injury,and typical HFMD symptoms.Notably,the nasal drip group exhibited a longer viral excretion cycle and more severe pathological damage.In the subsequent study,rhesus monkeys infected with CVB3 through nasal drips also presented signs of HFMD symptoms,viral excretion,serum antibody conversion,viral nucleic acids and antigens,and the specific organ damages,particularly in the heart.Surprisingly,there were no significant differences in myocardial enzyme levels,and the clinical symptoms resembled those often associated with common,mild infections.In summary,the study successfully developed severe Syrian hamsters and mild rhesus monkey models for CVB3-induced HFMD.These models could serve as a basis for understanding the disease pathogenesis,conducting pre-trial prevention and evaluation,and implementing post-exposure intervention.

关键词： Coxsackievirus B3(CVB3) Hand foot and mouth disease(HFMD) Animal models Syrian hamster Rhesus monkey

Early identification of stroke through deep learning with multi-modal human speech and movement data

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Neural Regeneration Research 2025年第1期20卷 234-241页

作者： zijun Ou Haitao Wang Bin Zhang Haobang Liang Bei Hu Longlong Ren Yanjuan liu Yuhu Zhang Chengbo Dai Hejun Wu Weifeng Li Xin Li School of Computer Science and Engineering Sun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Department of Neurology Guangdong Neuroscience InstituteGuangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhouGuangdong ProvinceChina Department of Emergency Medicine Guangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhouGuangdong ProvinceChina

Early identification and treatment of stroke can greatly improve patient outcomes and quality of life.Although clinical tests such as the Cincinnati Pre-hospital Stroke Scale(CPSS)and the Face Arm Speech Test(FAST)are commonly used for stroke screening,accurate administration is dependent on specialized training.In this study,we proposed a novel multimodal deep learning approach,based on the FAST,for assessing suspected stroke patients exhibiting symptoms such as limb weakness,facial paresis,and speech disorders in acute settings.We collected a dataset comprising videos and audio recordings of emergency room patients performing designated limb movements,facial expressions,and speech tests based on the FAST.We compared the constructed deep learning model,which was designed to process multi-modal datasets,with six prior models that achieved good action classification performance,including the I3D,SlowFast,X3D,TPN,TimeSformer,and MViT.We found that the findings of our deep learning model had a higher clinical value compared with the other approaches.Moreover,the multi-modal model outperformed its single-module variants,highlighting the benefit of utilizing multiple types of patient data,such as action videos and speech audio.These results indicate that a multi-modal deep learning model combined with the FAST could greatly improve the accuracy and sensitivity of early stroke identification of stroke,thus providing a practical and powerful tool for assessing stroke patients in an emergency clinical setting.

关键词： artificial intelligence deep learning diagnosis early detection FAST screening stroke

A calibration-free model for laser-induced breakdown spectroscopy using non-gated detectors

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Frontiers of physics 2022年第6期17卷 175-183页

作者： Zongyu Hou Weilun Gu Tianqi Li Zhe Wang Liang Li Xiang Yu Yecai Zhang zijun liu State Key Lab of Power System Department of Energy and Power EngineeringInternational Joint Laboratory on Low Carbon Clean Energy InnovationTsinghua UniversityBeijing 100084China Shanxi Research Institute for Clean Energy Tsinghua UniversityTaiyuan 030032China Beijing Research Institute of Chemical Engineering and Metallurgy Beijing 101149China China National Uranium Corporation Beijing 100013China Guoneng Shenwan Energy Co. LtdChina Jinneng Holding Tashan Power Generation Co. LtdChina

Calibration-free(CF)laser-induced breakdown spectroscopy(LIBS)is normally only applicable for gated detectors due to its dependence on the assumption of a steady-state plasma.However,most currently available LIBS systems are equipped with non-gated detectors such as chargecoupled device(CCD),which degrades the accuracy of CF method.In this paper,the reason for the less satisfactory quantification performance of CF for LIBS with non-gated detectors was clarified and a time-integration calibration-free(TICF)model was proposed for applications with non-gated detectors.It was based on an assumed temporal profile of plasma properties,including temperature and electron density,obtained from another pre-experiment.The line intensity at different time during the signal collection time window was estimated with self-absorption correction according to the temporal profile of the plasma properties.The proposed model was validated on titanium alloys and compared with traditional CF.The accuracy of elemental concentration measurement was improved significantly:the average relative error of aluminum and vanadium decreased from 6.07%and 22.34%to 2.01%and 1.92%,respectively.The quantification results showed that TICF method was able to extend the applicability of CF to LIBS with non-gated detectors.

关键词： laser-induced breakdown spectroscopy calibration-free nongated detector self-absorption correction

The Optimization Study about Fault Self-Healing Restoration of Power Distribution Network Based on Multi-Agent Technology

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Computers, Materials & Continua 2020年第10期65卷 865-878页

作者： Fuquan Huang zijun liu Tinghuang Wang Haitai Zhang Tony Yip Shenzhen Power Utility Co. Ltd.Shenzhen518000China Shandong Kehui Electric Power Automation Co. Ltd.Zibo255000China Kehui International Ltd. Studio 206Mill Studio Business CentreCrane MeadWareHertsSG129PYUK

In order to quickly and accurately locate the fault location of the distribution network and increase the stability of the distribution network,a fault recovery method based on multi-objective optimization algorithm is proposed.The optimization of the power distribution network fault system based on multiagent technology realizes fast recovery of multi-objective fault,solve the problem of network learning and parameter adjustment in the later stage of particle swarm optimization algorithm falling into the local extreme value dilemma,and realize the multi-dimensional nonlinear optimization of the main grid and the auxiliary grid.The system proposed in this study takes power distribution network as the goal,applies fuzzy probability algorithm,simplifies the calculation process,avoids local extreme value,and finally realizes the energy balance between each power grid.Simulation results show that the Multi-Agent Technology enjoys priority in restoring important load,shortening the recovery time of power grid balance,and reducing the overall line loss rate of power grid.Therefore,the power grid fault self-healing system can improve the safety and stability of the important power grid,and reduce the economic loss rate of the whole power grid.

关键词： Multi agent technology power distribution network fault self-healing