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Coating tetrahedral DNA framework with endosomolytic peptides for improved stability and cytosolic delivery

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Advanced Sensor and Energy Materials

Advanced Sensor and Energy Materials 2024年第2期3卷 1-5页

作者： Jinjun He Xiang Ji zihui xu Wei He Yan Zhao Lele Sun Lan Ma Institute of Biomedical Health Technology and Engineering Shenzhen Bay LaboratoryShenzhen 518132China Institute of Materiobiology Department of ChemistryCollege of ScienceShanghai UniversityShanghai 200444China Tsinghua-Berkeley Shenzhen Institute Tsinghua UniversityShenzhen518055 China Institute of Biopharmaceutical and Health Engineering Tsinghua Shenzhen International Graduate SchoolTsinghua UniversityShenzhen 518055China Division of Physical Biology Department CAS Key Laboratory of Interfacial Physics and TechnologyShanghai Institute of Applied PhysicsChinese Academy of SciencesShanghai 201800China State Key Laboratory of Chemical Oncogenomics Tsinghua Shenzhen International Graduate SchoolTsinghua UniversityShenzhen 518055China

DNA nanostructures have emerged as promising carriers for drug ***,challenges such as low stability,poor cellular uptake efficiency,and vulnerability to lysosomal degradation still hinder their therapeutic *** this study,we demonstrate the coating of tetrahedral DNA frameworks(TDF)with the endosomolytic peptide L17E through electrostatic interactions to address these *** findings highlight that L17E coating substantially enhances the stability of TDFs and improves their uptake efficiency into RAW264.7 cells through endocytosis and ***,L17E coating enables efficient endosomal release of ***,we employed L17E-coated TDF to deliver osteogenic growth peptide and demonstrated its potential applications in inhibiting periodontitis both in vitro and in *** straightforward and cost-effective strategy holds promise for advancing the biomedical applications of DNA nanostructures.

关键词： Tetrahedral DNA framework Endosomolytic peptides Stability Endosomal escape Efficient delivery

Finite-time synchronization of fractional Chen chaotic systems with different orders

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Finite-time synchronization of fractional Chen chaotic syste...

Curcumin inhibits proliferation of breast cancer cells through Nrf2-mediated down-regulation of Fen1 expression

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作者： Keyong Shao Xinyu Huang zihui xu Yujun Yan Institute of Electrical and Information Engineering Northeast Petroleum University

This paper discusses the finite-time synchronization of fractional-order Chen chaotic systems with different *** on the properties of fractional calculus,fractional-order systems with different orders are transformed into problems of the same order and a finite-time controller is designed by using the definition of finite-time synchronization,which makes the value of synchronization error system tend to zero in finite time,and then the finite-time synchronization of fractional-order chaotic systems with different orders is *** numerical simulation results verify the effectiveness and feasibility of the designed controller.

关键词： fractional-order chaotic system different order finite-time synchronization

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Curcumin inhibits proliferation of breast cancer cells throu...

Genome-wide antagonism between 5-hydroxymethylcytosine and DNA methylation in the adult mouse brain

第八届全国医学生物化学与分子生物学第五届全国临床应用生物化学与分子生物学2013华东六省一市生物化学与分子生物学联合学术研讨会

作者： Youzhi Zhang Yang Wang Jun Rao Xiaomei Jiang zihui xu Bin Chen Department of integrative medicine Xinqiao hospitalThird Military Medical University Department of Biochemistry and Molecular Biology Third Military Medical University

Curcumin can inhibit cell proliferation of breast cancer which mechanism remains *** of Flap endonuclease 1(Fen1),a DNA repair-specific nuclease,is related to the process of breast ***2 is a master regulator of cellular antioxidant defense systems,curcumin can induce the expression of Nrf2 in non breast cancer ***,whether curcumin-induced inhibition of breast cancer cell proliferation may be involved in Nrf2-mediated Fenl expression is not yet *** this study,we demonstrated that curcumin inhibited Fen1-dependent proliferation of MCF-7 cells,significantly induced Nrf2 protein expression while inhibited Fen1 protein *** investigation revealed that curcumin could lead to Nrf2 translocation from the cytoplasm to the nucleus and decrease Fenl promoter activity through lowing the recruitment of Nrf2 on Fenl *** data suggest that curcumin may inhibit the proliferation of MCF-7 cells through Nrf2-mediated down-regulation of Fen1 expression,which can provide a new mechanism for curcumin-induced tumor growth inhibition.

关键词： curcumin breast cancer Nrf2 Fenl

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Frontiers in Biology 2014年第1期9卷 66-74页

作者： Junjie U. GUO Keith E. SZULWACH Yijing SU Yujing LI Bing YAO zihui xu Joo Heon SH1N Bing XIE Yuan GAO Guo-li MING Peng JIN Hongjun SONG Institute for Cell Engineering Johns Hopkins University School of Medicine Baltimore MD 21205 USA The Solomon H. Snyder Department of Neuroscience Johns Hopkins University School of Medicine Baltimore MD 21205 USA Department of Neurology Johns Hopkins University School of Medicine Baltimore MD 21205 USA. Department of Human Genetics Emory University School of Medicine 615 Michael Street Atlanta GA 30322 USA Lieber Institute for Brain Development Johns Hopkins University School of Medicine Baltimore MD 21205 USA

Mounting evidence points to critical roles for DNA modifications, including 5-methylcytosine （5mC） and its oxidized forms, in the development, plasticity and disorders of the mammalian nervous system. The novel DNA base 5- hydroxymethylcytosine （5hmC） is known to be capable of initiating passive or active DNA demethylation, but whether and how extensively 5hmC functions in shaping the post-mitotic neuronal DNA methylome is unclear. Here we report the genome-wide distribution of 5hmC in dentate granule neurons from adult mouse hippocampus in vivo. 5hmC in the neuronal genome is highly enriched in gene bodies, especially in exons, and correlates with gene expression. Direct genome-wide comparison of 5hmC distribution between embryonic stem cells and neurons reveals extensive differences, reflecting the functional disparity between these two cell types. Importantly, integrative analysis of 5hmC, overall DNA methylation and gene expression profiles of dentate granule neurons in vivo reveals the genome-wide antagonism between these two states of cytosine modifications, supporting a role for 5hmC in shaping the neuronal DNA methylome by promoting active DNA demethylation.

关键词： dentate granule neuron active DNA demethylation TET methylome

二氮嗪干预氧化应激介导的INS-1细胞K_(ATP)通道作用机制的研究

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第三军医大学学报 2011年第11期33卷 1155-1158页

作者：朱静徐梓辉第三军医大学新桥医院内分泌科重庆400037

目的研究二氮嗪(diazoxide,DIZ)干预氧化应激对INS-1细胞ATP敏感性钾通道(Adenosine triphosphate-sensitive potassium channels,KATP channels)SUR1亚基基因的表达影响。方法将INS-1细胞株分为:①氧化应激组:将50、100、200、400μmol/L H2O2分别加入各瓶细胞中培养3 h;②二氮嗪干预组:加入50μmol/L二氮嗪培养INS-1细胞30 min后,加入400μmol/L H2O2,继续培养3 h;③牛磺酸干预组:加入1 mmol/L牛磺酸培养INS-1细胞30 min后,加入400μmol/L H2O2,继续培养3 h;④空白对照组:不加二氮嗪、牛磺酸及H2O2,其他培养条件相同。MTT检测氧化应激组细胞存活率、Elisa检测葡萄糖刺激的胰岛素分泌(glucose-stimulated insulin secretion,GSIS)、Western blot分别检测3组各SUR1亚基蛋白的表达。结果 H2O2处理INS-1细胞3 h与对照组相比较后50μmol/L组细胞存活率(105.27±3.83)%稍升高(P0.05)。结论低浓度H2O2可以促进细胞增殖与胰岛素分泌,中、高浓度H2O2抑制细胞增殖与胰岛素分泌,二氮嗪抑制细胞KATP通道SUR1亚基的表达,可能是通过抑制线粒体呼吸链电子转运抑制ROS的释放,减轻细胞氧化应激的程度。

关键词：胰岛细胞 KATP通道 SUR1亚基二氮嗪

A cognitive function predicted method by Useful Field of View tests among elder people in Beijing

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Journal of Traditional Chinese Medical Sciences 2017年第4期4卷 372-379页

作者： Aozhe Zhang Yan Lin Aimin Wang Yanan Zhao Yuming Zhang Huan Chen zihui xu xue Wang Rui Ni Qian Hua Beijing University of Chinese Medicine No.11North 3rd Ring RoadChaoyang DistrictBeijing100029China Tobacco Medicine and Tobacco Cessation Centre Centre of Respiratory MedicineChina-Japan Friendship HospitalNo 2East Yinghua roadChaoyang DistrictBeijing100029China Department of Psychology Wichita State University1845 FairmountWichitaKS 67260USA

Objective:To analyze the visual functions of the elder people in China by Useful Field of View (UFOV) to verify performances in Chinese ***:A total of 90 participants aged 60 years or above were recruited from residents in the Jiade Senior Apartment and the Fuchengmen Public Health Community in *** completed three tests,Mini Mental State Examination (NMSE),Digit Span test and *** also tested the digit span and UFOV the following ***:The correlation between age and MMSE score was significant (r =-0.422,P .1,while UFOV test 2 and UFOV test 3 showed significant positive relationships with ages,(both P <.01).Thirty-nine participants (out of 90) finished the digit span and UFOV the following *** UFOV test 3 has a significant differential performance between two years (t =-2.95,P <.01).We found UFOV tests showed the visual response capacity and had a strong correlation with ages (statistically significant).Conclusion:UFOV could be an effective way to evaluate cognitive ability in Chinese elderly people and the selective-attention test of UFOV may be a more sensitive measurement.

关键词： Age Cognitive Correlation analysis Elder people UFOV

双脱甲氧基姜黄素对db/db小鼠胰岛素抵抗和糖稳态作用及机制研究

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实用医学杂志 2018年第22期34卷 3685-3689页

作者：胡淑芳杨丽徐子辉武汉市汉口医院内分泌科武汉430012 华中科技大学同济医学院附属武汉中心医院内分泌科分子诊断湖北省重点实验室武汉430014

目的探讨双脱甲氧基姜黄素(BDMC)对db/db小鼠发生2型糖尿病(T2D)的作用及机制。方法 4周龄的db/db小鼠均衡随机分组为:db/db组、RG组和BDMC组,以C57BL/6为WT对照组。于每周检测空腹血糖,6周末置于监测其呼吸交换率和能量消耗变化,检测... 详细信息

目的探讨双脱甲氧基姜黄素(BDMC)对db/db小鼠发生2型糖尿病(T2D)的作用及机制。方法 4周龄的db/db小鼠均衡随机分组为:db/db组、RG组和BDMC组,以C57BL/6为WT对照组。于每周检测空腹血糖,6周末置于监测其呼吸交换率和能量消耗变化,检测糖化血红蛋白、胰岛素、血脂含量,行口服糖耐量试验,计算胰岛素抵抗的稳态指数和定量胰岛素敏感性检测指数,Western blot检测肝脏G6Pase、PEPCK和骨骼肌p-AMPK、AMPK、PM-GLUT4、GLUT4、pAS160、AS160蛋白表达水平。结果 BDMC明显下调db/db小鼠升高的体质量、饮食、饮水量和空腹血糖,上调db/db小鼠降低的呼吸交换比率和能量消耗量,且比RG作用更明显;BDMC对db/db小鼠升高的外周血糖化血红蛋白无显著作用,但RG组下调含量;BDMC上调db/db小鼠降低的胰岛素敏感性,下调db/db小鼠升高的血浆胰岛素、胰岛素抵抗的稳态指数和定量胰岛素敏感性检测指数;BDMC下调db/db小鼠升高的TG、TC、LDL-C、HDL-C和FFA;BDMC下调db/db小鼠肝脏高表达的G6Pase和PEPCK蛋白,且优于RG;BDMC上调db/db小鼠骨骼肌低表达的p-AMPK、PM-GLUT4和pAS160蛋白,与RG效应没有明显的差异。结论 BDMC可明显上调骨骼肌AMPK-AS160-GLUT4信号通路及下调肝脏G6Pase和PEPCK减低糖再生,改善db/db小鼠胰岛素抵抗和糖脂代谢减慢,且优于罗格列酮。

关键词：双脱甲氧基姜黄素糖尿病胰岛素抵抗糖稳态 AMPK信号通路

以不同体外模拟系统评价大豆蛋白的胃消化特性

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食品科学 2022年第18期43卷 69-75页

作者：吴凯云徐子惠郭健杨晓泉孟赫诚华南理工大学食品科学与工程学院食物蛋白与胶体研究中心广东省天然产物绿色加工与产品安全重点实验室广东广州510640

采用静态消化模型和半动态消化模型,评价经透析处理和未经透析处理的大豆蛋白的胃消化特性。结果表明:在静态消化模型中,透析对大豆蛋白的胃消化过程没有影响;但在半动态消化模型中,未经透析的大豆蛋白pH值、排空内容物的干物质质量和... 详细信息

采用静态消化模型和半动态消化模型,评价经透析处理和未经透析处理的大豆蛋白的胃消化特性。结果表明:在静态消化模型中,透析对大豆蛋白的胃消化过程没有影响;但在半动态消化模型中,未经透析的大豆蛋白pH值、排空内容物的干物质质量和游离氨基的含量与经透析处理的大豆蛋白存在一定差异,表明未经透析处理的大豆蛋白保留较多大豆中的成分,使其缓冲能力更强,胃排空滞后,蛋白质消化速率减慢。此外,静态消化模型和半动态消化模型有不同的优势,所以可以根据不同的研究目的采用不同的消化模型。这项工作将为大豆蛋白产品的开发和健康效应提供新的见解。

关键词：大豆蛋白透析静态消化模型半动态消化模型消化性能

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基于当代视觉艺术向度下锡伯族纹样的研究应用

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西部皮革 2024年第17期46卷 129-131页

作者：徐子蕙东北大学

民族纹样是少数民族文化中的一个重要组成部分。文章以锡伯族纹样为切入点，分析了不同民族风格中少数民族纹样在当代视觉设计中的应用。研究认为，锡伯族纹样以其特有的艺术效果向观者展现了中华文化的独特魅力，将少数民族文化融入当... 详细信息

民族纹样是少数民族文化中的一个重要组成部分。文章以锡伯族纹样为切入点，分析了不同民族风格中少数民族纹样在当代视觉设计中的应用。研究认为，锡伯族纹样以其特有的艺术效果向观者展现了中华文化的独特魅力，将少数民族文化融入当代视觉艺术，不仅可以创造出别具特色的设计风格，还能满足现代人对新事物的追求。

关键词：少数民族当代视觉艺术锡伯族纹样融合