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Dynamic intrauterine crosstalk promotes porcine embryo implantation during early pregnancy

Science China(life Sciences) 2024年第8期67卷 1676-1696页

作者： Xupeng Zang Shengchen Gu Wenjing Wang Junsong Shi Jianyu Gan Qun Hu Chen Zhou Yue Ding Yanjuan He Lei Jiang Ting Gu Zheng Xu Sixiu Huang Huaqiang Yang Fanming Meng zicong li Gengyuan Cai linjun Hong Zhenfang Wu State Key Laboratory of Swine and Poultry Breeding Industry National Engineering Research Center for Breeding Swine IndustryCollege of Animal ScienceSouth China Agricultural UniversityGuangzhou 510642China Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding South China Agricultural UniversityGuangzhou 510642China Yunfu Subcenter of Guangdong Laboratory for lingnan Modern Agriculture Yunfu 527300China Guangdong Key Laboratory of Animal Breeding and Nutrition Institute of Animal ScienceGuangdong Academy of Agricultural SciencesGuangzhou 510640China Key Laboratory of South China Modern Biological Seed Industry Ministry of Agriculture and Rural AffairsGuangzhou 510520China

Dynamic crosstalk between the embryo and mother is crucial during ***,we comprehensively profile the single-cell transcriptome of pig peri-implantation embryos and corresponding maternal endometrium,identifying 4 different lineages in embryos and 13 cell types in the ***-specific gene expression characterizes 4 distinct trophectoderm subpopulations,showing development from undifferentiated trophectoderm to polar and mural *** expression of genes in different types of endometrial cells illustrates their molecular response to embryos during ***,we developed a novel tool,ExtraCellTalk,generating an overall dynamic map of maternal-foetal crosstalk using uterine luminal proteins as *** cross-species comparisons,we identified a conserved RBP4/STRA6 pathway in which embryonic-derived RBP4 could target the STRA6 receptor on stromal cells to regulate the interaction with other endometrial *** results provide insight into the maternal-foetal crosstalk during embryo implantation and represent a valuable resource for further studies to improve embryo implantation.

关键词： endometrial remodeling ExtraCellTalk implantation maternal-foetal crosstalk trophoblast

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树脂黏度对碳纤维与环氧树脂浸润性及界面结合性能的影响

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化工新型材料 2024年第6期52卷 149-154页

作者：韩亚磊章桥新高列义赵飞李资聪郑磊武汉理工大学材料科学与工程学院武汉430070 湖北航天技术研究院总体设计所武汉430048

碳纤维与环氧树脂基体形成良好的界面结合是发挥复合材料基本性能的保障,考察3种国产T700级碳纤维,研究树脂黏度对碳纤维浸润性以及界面结合性能的影响。结果表明:由室温升到60℃,树脂体系黏度从1401.9mPa·s降低到102.77mPa·s,碳纤维... 详细信息

碳纤维与环氧树脂基体形成良好的界面结合是发挥复合材料基本性能的保障,考察3种国产T700级碳纤维,研究树脂黏度对碳纤维浸润性以及界面结合性能的影响。结果表明:由室温升到60℃,树脂体系黏度从1401.9mPa·s降低到102.77mPa·s,碳纤维与树脂的接触角减小,浸润能力增强,从而提高复合材料的界面结合强度,在60℃下制备的复合材料层间剪切强度提高了17.44%。同时纤维表面的沟槽结构能够加强物理机械互锁作用,加强复合材料的界面结合强度。

关键词：碳纤维复合材料黏度浸润性界面结合性能层间剪切强度

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硫酸盐胁迫下AnMBR系统中亚可见颗粒物的生成及其膜污染行为

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环境工程学报 2024年第5期18卷 1265-1272页

作者：李自聪陈子健周忠波陈玉成孟凡刚西南大学资源环境学院环境科学与工程系重庆400716 北控水务(中国)投资有限公司北京100015 广州行政学院广州510070 中山大学环境科学与工程学院广州510006

硫酸盐废水的厌氧生物处理一直是研究热点,关于硫酸盐对厌氧膜生物反应器(AnMBR)污泥混合液性质和上清液颗粒物组成及其膜污染行为的影响仍不明确。设置了3组AnMBRs,分别在不同COD/SO_(4)^(2-)比值(>10、2.5和0.5)下运行约200 d,探究了... 详细信息

硫酸盐废水的厌氧生物处理一直是研究热点,关于硫酸盐对厌氧膜生物反应器(AnMBR)污泥混合液性质和上清液颗粒物组成及其膜污染行为的影响仍不明确。设置了3组AnMBRs,分别在不同COD/SO_(4)^(2-)比值(>10、2.5和0.5)下运行约200 d,探究了硫酸盐胁迫下AnMBR系统性能和上清液亚可见颗粒物(0.45~10µm)的产生及其膜污染行为。结果表明,随着COD/SO_(4)^(2-)比值的降低,上清液中COD值明显上升;当COD/SO_(4)^(2-)比值为0.5时,上清液COD增至近4000 mg·L^(−1),H_(2)S比例则高达约1.6%,产甲烷被严重抑制。相应地,高硫酸盐胁迫下跨膜压差TMP的增长速率显著增加。通过死端过滤实验发现,在相同TSS浓度下,高硫酸盐AnMBR系统中污泥混合溶液呈现出最差的过滤性能,其中上清液过滤性能与污泥混合液几乎相同;在相同COD值下高硫酸盐系统上清液过滤速度最慢,且膜阻力最高,滤饼层阻力占比高于95%。颗粒物尺寸分布分析结果表明,高硫酸盐胁迫下AnMBR上清液中亚可见颗粒物的占比大大增加(小于10µm的颗粒物占比高达75%~80%);相比于污泥上清液,在高硫酸盐胁迫下AnMBR膜表面滤饼层中0.45~1µm和1~5µm的亚可见颗粒物具有较高的累积潜能。以上研究结果可为进一步优化膜污染控制提供参考。

关键词：厌氧膜生物反应器膜污染硫酸盐微细颗粒物

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怀孕后期饲喂利司培酮对母鼠乳腺发育及其幼鼠生长的影响

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中国畜牧兽医 2024年第6期51卷 2489-2500页

作者：王顺博王浩梁韫怡王雯竞李紫聪徐铮华南农业大学动物科学学院国家生猪种业工程技术研究中心广州510642 岭南师范学院生命科学与技术学院湛江524000

【目的】本研究以小鼠作为试验对象,探究利司培酮能否促进哺乳动物乳腺发育及后代生长。【方法】将20只雌性昆明小鼠分为2组,每组10只。试验组小鼠在怀孕后期饲喂含0.25 mg/L利司培酮的饮水,连续饲喂4 d,对照组小鼠饲喂不含利司培酮的... 详细信息

【目的】本研究以小鼠作为试验对象,探究利司培酮能否促进哺乳动物乳腺发育及后代生长。【方法】将20只雌性昆明小鼠分为2组,每组10只。试验组小鼠在怀孕后期饲喂含0.25 mg/L利司培酮的饮水,连续饲喂4 d,对照组小鼠饲喂不含利司培酮的饮水。每组在哺乳第3和18天各采集5只小鼠乳腺组织及血液。应用EliSA方法检测血清中催乳素(PRL)水平,HE染色法观察乳腺组织形态学变化,通过称重和计数方法统计2组间母鼠乳腺重量及幼鼠平均体重和存活率差异情况,对2个时期的乳腺进行转录组测序,并对测序数据进行GO、KEGG和GSEA富集分析。【结果】哺乳第3天时利司培酮组小鼠血清PRL水平极显著高于对照组(P<0.01);哺乳第3和18天利司培酮组小鼠乳腺丰富程度高于对照组;哺乳第18天时利司培酮组母鼠乳腺重量及其幼鼠平均体重和存活率均显著高于对照组(P<0.05);GO功能富集分析结果显示,哺乳第3天2组小鼠乳腺组织的差异表达基因主要集中在脂肪酸代谢、脂肪细胞分化等生物过程,哺乳第18天利司培酮处理组母鼠的差异表达基因主要集中在脂肪酸代谢过程的负调控、组织重塑等生物过程;KEGG通路富集分析结果显示,哺乳第3天的差异表达基因主要富集于PPAR信号通路、cAMP信号通路、脂肪消化吸收等途径,哺乳第18天的差异表达基因主要在PPAR信号通路、脂肪细胞脂解作用的调控等途径最为富集;GSEA富集分析结果显示,哺乳第3和18天两时期的利司培酮处理组均富集到了雌激素和PRL信号通路。【结论】给怀孕后期母鼠饲喂利司培酮,可促进母鼠乳腺发育和幼鼠生长。本研究揭示了利司培酮对小鼠乳腺发育的促进作用,为将来建立在怀孕后期母畜饮水中添加利司培酮来提高泌乳和促进后代生长的新方法奠定基础,对于促进哺乳动物繁殖和养殖效率提高具有重要意义。

关键词：利司培酮小鼠乳腺转录组测序

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基因修饰技术及其在动物育种和生物医药领域的应用

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中国畜牧兽医 2024年第2期51卷 659-667页

作者：闫艳霞李紫聪董亚铮李政黄思秀华南农业大学动物科学学院广州510642 国家生猪种业工程技术研究中心广州510642

基因修饰技术是一种能精确改造生物基因组,实现外源基因定点整合和基因定点敲除的技术。早期的基因修饰形式主要是转基因,随着科学研究的不断深入,新型基因修饰方法也逐渐研发出来,包括敲除、敲入、定点突变等。根据研究或应用的目的,... 详细信息

基因修饰技术是一种能精确改造生物基因组,实现外源基因定点整合和基因定点敲除的技术。早期的基因修饰形式主要是转基因,随着科学研究的不断深入,新型基因修饰方法也逐渐研发出来,包括敲除、敲入、定点突变等。根据研究或应用的目的,可以将基因修饰技术分为转基因和基因敲除两方面内容。近年来,随着现代分子技术的高速发展,基因修饰技术不断改进创新,其相关方法和技术已逐步应用于改良家畜性状、研究基因功能、制作动物生物反应器以及构建人类疾病动物模型等领域中,使得畜禽基因功能的研究和转基因育种更加高效,在动物遗传育种以及生物医药等领域取得了显著成就,弥补了传统转基因技术的随机整合、遗传不稳定等缺陷,具有广阔的发展前景。作者从动物转基因和基因敲除技术两方面阐述了基因修饰技术的发展现状及发展趋势,并简要概括了基因修饰技术在动物育种和生物医药领域的应用现状。

关键词：基因修饰转基因技术基因敲除技术

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Brassinosteroid Signaling Recruits Histone 3 Lysine-27 Demethylation Activity to FLOWERING LOCUS C Chromatin to Inhibit the Floral Transition in Arabidopsis

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Molecular Plant 2018年第9期11卷 1135-1146页

作者： zicong li Yang Ou Zhicheng Zhang Jianming li Yuehui He Shanghai Center for Plant Stress Biology & National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant Sciences Chinese Academy of Sciences (CAS) Shanghai 201602 China University of Chinese Academy of Sciences Beijing 100049 China Shanghai Center for Plant Stress Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 201602 China These authors contributed equally to this article

The steroid hormone brassinosteroid （BR） plays a broad role in plant growth and development. As the retarded growth in BR-insensitive and BR-deficient mutants causes a strong delay in days to flowering, BR signaling has been thought to promote the floral transition inArabidopsis. In this study, using a developmental measure of flowering time, we show that BR signaling inhibits the floral transition and promotes vegetative growth in the Arabidopsis accessions Columbia and Enkheim-2. We found that BR signaling promotes the expression of the potent floral repressor FLOWERING LOCUS C （FLC） and three FLC homologs to inhibit flow- ering. In the presence of BR, the transcription factor BRASSINAZOLE-RESISTANT1 （BZR1）, together with BES1 -INTERACTING MYC-like proteins （BIMs）, specifically binds a BR- responsive element in the first intron of FLC and further recruits a histone 3 lysine 27 （H3K27） demethylase to downregulate levels of the repressive H3K27 trimethylation mark and thus antagonize Polycomb silencing at FLC, leading to its activation. Taken together, our findings demonstrate that BR signaling inhibits the floral transition inArabidopsis by a novel molecular mechanism in which BR signals are transduced into FLC activation and consequent floral repression.

关键词： brassinosteroid BR signaling flowering time BZR1 Polycomb silencing FLOWERING LOCUS C

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JASMONATE-ZIM DOMAIN proteins engage Polycomb chromatin modifiers to modulate Jasmonate signaling in Arabidopsis

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Molecular Plant 2021年第5期14卷 732-747页

作者： zicong li Xiao Luo Yang Ou Huijun Jiao li Peng Xing Fu Alberto P.Macho Renyi liu Yuehui He Shanghai Center for Plant Stress Biology Chinese Academy of Sciences Center for Excellence in Molecular Plant SciencesShanghai 201602China Ministry of Education Key Laboratory of Plant Development and Environmental Adaption Biology School of Life SciencesShandong UniversityQingdao 266237China Department of Biological Sciences National University of SingaporeSingapore 117543Singapore Peking University Institute of Advanced Agricultural Sciences WeifangShandong 261000China Center for Agroforestry Mega Data Sciences Haixia Institute of Science and TechnologyFujian Agriculture and Forestry UniversityFuzhou 350002China State Key Laboratory of Protein and Plant Gene Research Peking-Tsinghua Center for Life SciencesSchool of Advanced Agriculture SciencesPeking UniversityBeijing 100871China

Jasmonate(JA)regulates various aspects of plant growth and development and stress responses,with prominent roles in male reproductive development and defenses against herbivores and necrotrophic ***-ZIM DOMAIN(JAZ)proteins are key regulators in the JA signaling pathway and function to repress the expression of JA-responsive ***,we show that JAZ proteins directly interact with several chromatin-associated Polycomb proteins to mediate repressive chromatin modifications at JA-responsive genes and,thus,their transcriptional repression in *** analyses revealed that the developmental defects,including anther and pollen abnormalities,resulting from loss or block of JA signaling were partially rescued by loss of Polycomb protein-mediated chromatin silencing(Polycomb repression).We further found that JAZ-mediated transcriptional repression during anther and pollen development requires Polycomb proteins at four key Regulatory *** of genome-wide occupancy of a Polycomb factor and transcriptome reprogramming in response to JA revealed that Polycomb repression is involved in the repression of various JA-responsive *** together,our study reveals an important chromatin-based mechanism for JAZ-mediated transcriptional repression and JA signaling in plants.

关键词： jasmonate JA response Polycomb proteins JAZ proteins anther development H3K27me3

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Injection of H3K9me3 demethylase Kdm4b/d mRNA improves the in vitro developmental ability of cloned porcine embryos.

Injection of H3K9me3 demethylase Kdm4b/d mRNA improves the i...

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2015中国遗传学会大会

作者： Guo li zicong li Dewu liu Zengfang Wu National Engineering Research Center for Swine Breeding Industry/College of Animal Science South China Agricultural University

The pig somatic cell nuclear transfer(SCNT)technique has valuable applications in agriculture as well as biomedical ***,the efficiency of this technique currently is still very *** porcine embryos derived from SCNT only have a full term developmental rat at about 1%.The majority of porcine SCNT embryos fail to develop to term due to undefined reprogramming *** it have been reported that removal of H3K9me3 in cloned mouse embryos by ectopically expressed H3K9me3 demethylase Kdm4d not only reactivates genes suppressed by H3k9me3,but also increases mouse SCNT efficiency to 8.2%.Nevertheless,whether decrease of H3K9me3 level also can improve the developmental rate of cloned porcine embryos is still unknown *** purpose of this study was to explore the effect of over-expression of Kdm4b/d on pig SCNT embryos’developmental ***4d and Kdm4b mRNA was respectively microinjected into 150 cloned porcine embryos at one-cell stage,and their in vitro developmental indexes were *** results showed that embryos’cleavage rate and blastocyst rate are not significantly different between two injection groups and non-injected control ***,embryos injected with Kdm4b mRNA have significantly higher cell number at blastocyst stage(35.63±0.02)as compared to control embryos(28.98±0.07),although Kdm4d mRNA-injected embryos have similar blastocyst cell number with control *** suggests that injection of Kdm4b mRNA improves the in vitro developmental efficiency of pig SCNT *** studies will use immunofluorescence to investigate the level of H3K9me3 in injected cloned porcine embryos.

关键词： SCNT H3K9me3 cloned embryos Kdm4b/d pigs cloning efficiency

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Comparison of birth weight and umbilical and placental characteristics of cloned and arti?cial insemination-derived piglets

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Frontiers of Agricultural Science and Engineering 2019年第1期6卷 54-60页

作者： Zheng AO Chengfa ZHAO Yanmin GAN Xiao WU Junsong SHI Enqin ZHENG Dewu liU Gengyuan CAI Zhenfang WU zicong li National Engineering Research Center for Breeding Swine Industry College of Animal Science South China Agricultural University Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding College of Animal Science South China Agricultural University Guangdong Wen’s Breeding Swine Co. Ltd.

Somatic cell nuclear transfer(SCNT)-derived piglets have signi?cantly higher stillbirth rate and postnatal mortality rate than arti?cial insemination(AI)-generated piglets. The question whether the low survival rate of SCNT piglets was related to birth weight, umbilical cord or placenta development was investigated. In this study,stillbirth rate, neonatal death rate, birth weight, umbilical cord status, placental parameters and placental gene expression patterns were compared between SCNT and AI piglets. Results showed that mortality rates at birth and during the neonatal stage of SCNT piglets were signi?-cantly higher than those of AI piglets. The incidence of abnormal umbilical cord in SCNT and SCNT-liveborn(SCNT-LB) piglets was signi?cantly higher than in AI and AI-liveborn(AI-LB) piglets. Birth weight, placental weight, placental surface area and placental ef?ciency in SCNT and SCNT-LB piglets were signi?cantly lower than those of AI and AI-LB piglets. Placental expression pro?les of imprinting, angiopoiesis and nutrient transportrelated genes were defective in SCNT-LB piglets compared with those in AI-LB piglets. Thus, the low survival rate of SCNT piglets may be associated with abnormal umbilical cord and placenta development. These characteristics may have resulted from aberrant expression of angiogenesis, nutrient transport, and imprinting-related genes in the placentas.

关键词： cloned pig death placenta SCNT umbilical cord

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Effects of enucleation method on in vitro and in vivo development rate of cloned pig embryos

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Frontiers of Agricultural Science and Engineering 2019年第1期6卷 61-65页

作者： Chengcheng ZHAO Junsong SHI Rong ZHOU Ranbiao MAI Lvhua LUO Xiaoyan HE Hongmei JI Gengyuan CAI Dewu liU Enqin ZHENG Zhenfang WU zicong li National Engineering Research Center for Breeding Swine Industry College of Animal Science South China Agricultural University Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding College of Animal Science South China Agricultural University Guangdong Wens Foodstuff Group Co. Ltd.

Enucleation is a crucial procedure for mammalian somatic cell nuclear transfer(SCNT), especially for domestic animal cloning. Oocytes of domestic animals such as pigs and cattle contain dark lipid droplets that hinder localization and removal of the nucleus. Using an oocyte enucleation technique that can obtain a high enucleation rate but has minimal negative effects on the reprogramming potential of oocyte for cloning is bene?cial for enhancing the outcome of SCNT. In this study, we compared the pig cloning ef?ciency resulting from blind aspiration-based(BA-B) enucleation and spindle imaging system-assisted(SIS-A) enucleation, and compared the pig SCNT success rate associated with BA-B enucleation and blind aspiration plus post-enucleation staining-based(BAPPS-B) enucleation. SIS-A enucleation achieved a signi?cantly higher oocyte enucleation success rate and tended to obtain a higher in vivo full term development rate of SCNT embryos than BA-B enucleation. BAPPS-B enucleation also obtained signi?cantly higher in vitro as well as in vivo full term development ef?ciency of cloned porcine embryos than BA-B enucleation. These data indicate that SIS-A and BAPPS-B enucleation are better approaches for pig SCNT than BA-B enucleation.

关键词： cloning enucleation pig SCNT

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