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超声辅助酶解制备草鱼鳞胶原肽及其理化特性评价

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现代食品科技 2022年第9期38卷 159-170页

作者：赵钰余小月熊喆秦子波王希搏荣建华熊善柏胡杨华中农业大学食品科学技术学院国家大宗淡水鱼加工技术研发分中心湖北武汉430070 生物活性肽技术湖北省工程研究中心湖北荆州434000

以草鱼鳞为原料,基于研究室原有工艺条件,在酶解过程中施加超声,研究超声功率(0∼600 W)和超声时间(0∼40 min)对胶原肽得率及理化特性的影响。结果表明,超声对产物得率影响显著。单酶酶解使用碱性蛋白酶、复合蛋白酶、中性蛋白酶。超... 详细信息

以草鱼鳞为原料,基于研究室原有工艺条件,在酶解过程中施加超声,研究超声功率(0∼600 W)和超声时间(0∼40 min)对胶原肽得率及理化特性的影响。结果表明,超声对产物得率影响显著。单酶酶解使用碱性蛋白酶、复合蛋白酶、中性蛋白酶。超声条件增加,水解度和氮收率先升高后降低;在碱性蛋白酶酶解时施加300 W、20 min超声,水解度从5.37%升到7.27%;分步酶解使用碱性蛋白酶和风味酶,在每步酶解各施加300 W、10 min超声时,水解度从9.26%升到11.05%。超声对产物分子量和氨基酸含量有一定影响,产物分子量集中在500 u~1 ku,单酶酶解胶原肽在该段分布从24.26%升至33.99%,分步酶解胶原肽在该段分布从31.99%升至39.28%;单酶酶解氨基酸含量从66.30 g/100 g升至73.75 g/100 g;分步酶解从66.05 g/100 g升至70.70 g/100 g。超声对产物乳化性、起泡性和泡沫稳定性影响显著。综上,单酶酶解最佳工艺为碱性蛋白酶酶解中施加300 W、20 min超声;分步酶解最佳工艺为在每步酶解中各施加功率300 W、时间10 min的超声。

关键词：草鱼鳞超声水解度氮收率胶原肽理化特性

机器人辅助经皮内镜腰椎椎间融合术治疗腰椎管狭窄症合并腰椎节段不稳

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中华骨科杂志 2024年第16期44卷 1061-1068页

作者：陈博来林涌鹏李永津苏国义高梓博林锐胡伟雄广东省中医院(广州中医药大学第二附属医院)脊柱微创科广州510120

目的探讨机器人辅助经皮内镜腰椎椎间融合术治疗腰椎管狭窄症合并腰椎节段不稳的有效性和安全性。方法2018年9月至2022年4月于广东省中医院脊柱微创科行机器人辅助经皮内镜腰椎椎间融合术治疗腰椎管狭窄症合并腰椎节段不稳患者26例,男1... 详细信息

目的探讨机器人辅助经皮内镜腰椎椎间融合术治疗腰椎管狭窄症合并腰椎节段不稳的有效性和安全性。方法2018年9月至2022年4月于广东省中医院脊柱微创科行机器人辅助经皮内镜腰椎椎间融合术治疗腰椎管狭窄症合并腰椎节段不稳患者26例,男13例、女13例,年龄(57.92±7.09)岁(范围44~75岁),体质指数为(24.05±2.64)kg/m^(2)(范围19.38~29.06 kg/m^(2))。共计31个节段包括单节段手术21例(L 3,42例、L 4,518例、L 5S 11例)、双节段手术5例(L 3~L 5)。术前,术后1、6、12个月及末次随访时采用疼痛视觉模拟评分(visual analogue scale,VAS)评估腰痛和下肢痛程度,采用Oswestry功能障碍指数(Oswestry disability index,ODI)评估脊椎神经功能;末次随访时采用MacNab标准评估临床疗效。影像学评价指标包括手术前后手术节段椎间隙高度、腰椎前凸角、融合率及椎弓根螺钉准确度。结果26例随访时间为(42.88±10.09)个月(范围12~55个月)。手术时间为(156.54±33.50)min,术后引流量为(27.23±20.20)ml。术前腰痛和下肢痛VAS为(4.35±1.23)、(6.08±0.63)分,术后1个月降至(2.08±0.69)、(1.85±0.54)分,术后6个月为(1.85±0.54)、(0.77±0.59)分,术后1年为(0.96±0.53)、(0.62±0.57)分,末次随访为(0.88±0.52)、(0.58±0.50)分,差异均有统计学意义(F=85.943,P=0.001;F=547.946,P=0.014)。术前,术后1、6、12个月及末次随访的ODI分别为55.38%±5.89%、28.38%±3.849%、17.77%±2.67%、12.58%±1.88%、12.12%±2.27%,差异有统计学意义(F=783.289,P=0.010)。根据MacNab标准末次随访时26例中优18例、良6例、可2例,优良率为92.3%。机器人辅助植入经皮椎弓根螺钉114枚,A级109枚、B级4枚、C级1枚。术前、术后及末次随访椎间隙高度分别为(10.55±1.96)、(13.53±1.37)、(12.54±1.42)mm,差异有统计学意义(F=42.190,P0.05)。术后12个月获得骨性融合25例,融合率为96%(25/26)。结论机器人辅助经皮内镜腰椎椎间融合术治疗腰椎管狭窄症合并腰椎节段不稳能有效改善手术节段的椎间隙高度,是一种安全有效的微创手术方式。

关键词：机器人椎管狭窄脊柱融合术腰椎不稳

Characteristics of interfacial reactions between Ti-6Al-4V alloy and ZrO2 ceramic mold

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China Foundry 2020年第6期17卷 409-415页

作者： Shi-chen Sun Er-tuan Zhao Chen hu Jin-rui Yu Yu-kun An Ren-guo Guan School of Mechanical Engineering Shandong University of TechnologyZibo 255022China School of Materials Science and Engineering Dalian JiaoTong UniversityDalian 116028China

The interfacial reaction between Ti-6Al-4V alloy and ZrO2 ceramic mold with zirconia sol binder was investigated by keeping the 12 g alloy melt in a vacuum induction furnace for 15 *** microstructures,element distribution and phase constitution of the interface were identified by optical microscopy(OM),scanning electron microscopy(SEM)equipped with energy dispersive spectroscopy(EDS)and X-ray diffraction(XRD).The results show that the whole interface reaction layer can be divided into three regions:metal penetration layer,transition layer,and hardened layer according to the structure morphology,which has the characteristics of severe metal penetration,finer lamellar,and coarse oxygen-richαphase,*** erosion of the alloy melt on the ceramic mold promotes the decomposition of zirconia,which leads to the increase of local Zr concentration,greatly increasing the activity coefficient of Ti,aggravating the occurrence of interfacial ***,the interfacial reaction shows the characteristics of chain *** the oxygen released by the dissolution of zirconia exceeds the local solid solubility,it precipitates in the form of bubbles,resulting in blowholes at the *** result also indicates that the zirconia mold with zirconia sol binder is not suitable for pouring heavy titanium alloy castings.

关键词： titanium alloy interfacial reaction ZrO2 ceramic mold activity coefficient

水压力与地应力作用下岩石透射波幅值特性试验研究

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矿业研究与开发 2022年第11期42卷 132-139页

作者：金解放石子勃胡玮锋孙俊涛杨洪灏刘康江西理工大学土木与测绘工程学院江西赣州市341099 江西理工大学资源与环境工程学院江西赣州市341099

深部资源开采和深埋隧洞等岩体工程多处于高水压高地应力环境中,在爆破开挖和地震灾害等动态荷载扰动下,动荷载以应力波形式在岩体中传播,研究岩石应力波传播特性是分析评估动荷载作用下围岩体稳定性的基础。为研究不同大小水压和地应... 详细信息

深部资源开采和深埋隧洞等岩体工程多处于高水压高地应力环境中,在爆破开挖和地震灾害等动态荷载扰动下,动荷载以应力波形式在岩体中传播,研究岩石应力波传播特性是分析评估动荷载作用下围岩体稳定性的基础。为研究不同大小水压和地应力对岩石应力波传播特性的影响,利用改进的SHPB试验系统,对饱和红砂岩开展了应力波传播试验研究,设置6种水压力和4种轴向静应力等级,分析透射波幅值随水压力和轴向静应力的变化关系,并构建其经验模型。结果表明:当冲击速度和轴向静应力一定时,随着水压的增高,透射波幅值呈幂函数增加,但增加的幅度越来越小,这是由于水压本身的侧向约束作用、弯液面效应和Stefan效应抑制了岩石内部孔隙的扩展,加强了应力波在岩石中的穿透能力;当水压一定时,轴向静应力越大,透射波幅值越小;轴向静应力越大,透射波幅值随水压增加的幅度越大。研究结果可为深部高水压工程岩体爆破开挖时的围岩体稳定性分析提供理论依据。

关键词：岩石应力波传播高水压力高地应力透射波幅值

Understanding water conservation vs. prof ligation traits in vegetable legumes through a physio-transcriptomic-functional approach

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Horticulture Research 2023年第3期10卷 28-40页

作者： Pingping Fang Ting Sun Arun Kumar Pandey Libo Jiang Xinyang Wu Yannan hu Shiping Cheng Mingxuan Li Pei Xu College of Life Sciences China Jiliang UniversityXueyuan Street No.258Hangzhou 310018China School of Life Sciences and Medicine Shandong University of TechnologyXincun West Road No.255Zibo 255000China Henan Provincial Key Lab of Germplasm Innovation and Utilization of Eco-economic Woody Plant Pingdingshan UniversityWeilai Street No.1Pingdingshan 467000China Key Laboratory of Specialty Agri-Product Quality and Hazard Controlling Technology of Zhejiang China Jiliang UniversityXueyuan Street No.258Hangzhou 310018China

Abstract Vegetable soybean and cowpea are related warm-season legumes showing contrasting leaf water use behaviors under similar root drought stresses,whose mechanisms are not well *** we conducted an integrative phenomic-transcriptomic study on the two crops grown in a feedback irrigation system that enabled precise control of soil water *** transpiration rate monitoring demonstrated that cowpea used water more conservatively under earlier soil drought stages,but tended to maintain higher transpiration under prolonged ***,we observed a soybean-specific transpiration rate increase accompanied by phase shift under moderate soil ***-series transcriptomic analysis suggested a dehydration avoidance mechanism of cowpea at early soil drought stage,in which the VuHAI3 and VuTIP2;3 genes were suggested to be *** gene clustering analysis revealed different responsiveness of genes to drought,time of day and their interactions between the two crops,which involved species-dependent regulation of the circadian clock *** network analysis identified two co-expression modules each associated with transpiration rate in cowpea and soybean,including a pair of negatively correlated modules between *** hub genes,including the ABA-degrading gene GmCYP707A4 and the trehalose-phosphatase/synthase gene VuTPS9 were *** network analysis revealed putative co-players of the hub *** analyses verified the role of VuTPS9 in regulating transpiration rate under osmotic *** findings propose that species-specific transcriptomic reprograming in leaves of the two crops suffering similar soil drought was not only a result of the different drought resistance level,but a cause of it.

关键词： soybean soil crops

Effect of Targeted Nursing Based on Basic Living Care on Patients With Chronic Gastric Ulcer

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Proceedings of Anticancer Research 2020年第1期4卷 9-11页

作者： Xue hu Department of Internal Medicine Maternal and Childcare Hospital of Zibo CityZibo 255000Shandong ProvinceChina

Objective:To explore the effect of targeted nursing based on basic living care on patients with chronic gastric ***:Ninety patients with chronic gastric ulcer treated in our hospital from August 2018 to August 2019 were selected and divided into observation group(n=45)and control group(n=45)*** control group was given routine *** the basis of the control group,the observation group was given targeted care based on basic living *** the compliance behavior in two ***:After the intervention,the compliance score of the observation group was higher than that of the control group,and the difference was statistically significant(P<0.05).Conclusion:The use of targeted care based on basic living care for patients with chronic gastric ulcer can improve their compliance behavior,which is worthy of clinical application.

关键词： Chronic gastric ulcer Targeted nursing based on basic living care Compliance behavior

Nursing Effect of Standardized Nursing Intervention in Endoscopic Examination of Patients with Gastric Ulcer Complicated with Diabetes

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Journal of Clinical and Nursing Research 2020年第3期4卷 33-35页

作者： Xue hu Department of Internal Medicine Maternal and Child Health Hospital of Zibo CityZibo 255000Shandong ProvinceChina

Objective:To explore the nursing effect of standardized nursing interventions in endoscopic examination of patients with gastric ulcer complicated with ***:90 patients with gastric ulcer complicated with diabetes who were treated in our hospital from May 2018 to May 2019 were selected and randomly divided into 2 groups,each containing *** control group received routine nursing,and the observation group performed standardized nursing intervention on the basis of the control *** control group received conventional nursing,and the observation group performed standardized nursing intervention on the basis of the control *** nursing,the two groups of patients were compared for their psychological state and nursing ***:After nursing,the HAMD and HAMA scores of the two groups were lower than before the nursing,and the observation group was lower than the control group,the difference was statistically significant(P<0.05).Conclusion:Standardized nursing intervention can alleviate the negative emotion of endoscopic examination of patients with gastric ulcer complicated with diabetes,which is conducive to the smooth progress of the examination.

关键词： Gastric ulcer with diabetes Standardized nursing intervention Endoscopy Psychological state

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以人民为中心的体育主体性的实践逻辑

体育科学 2021年第12期41卷 30-38,F0003页

作者：张业安李崟杜恺胡德平郑国华王资博刘建上海体育学院上海200438

以人民为中心的发展思想把人民作为实践主体、认识主体、价值主体、历史主体。基于以人民为中心的发展思想,依据人民群体内涵的广泛性特征,以人民为中心的体育主体性的实践逻辑从不同人民群体的体育主体性出发,凸显“人民中心性”,使我... 详细信息

以人民为中心的发展思想把人民作为实践主体、认识主体、价值主体、历史主体。基于以人民为中心的发展思想,依据人民群体内涵的广泛性特征,以人民为中心的体育主体性的实践逻辑从不同人民群体的体育主体性出发,凸显“人民中心性”,使我国体育事业改革发展既作为人民个体成长和实现幸福生活的重要途径,亦呼应国家战略和民族发展需求,成为国家富强、民族复兴、人类进步的重要载体和内容。因此,基于“满足人民群众对美好生活的向往”和“通过体育促进人的全面发展”两个视角,从“城乡一体化推进”和“全人群全生命周期覆盖”两个层面提出具体实践路径:遵循“共建、共治、共享”理念,实现人民体育与人民城市建设相融合;实施“乡村体育振兴战略”,凸显农民主体性,提升农民幸福感;以体教融合促进儿童青少年健康发展;以体养融合提供健康老龄化的体育解决方案;以强化女性体育参与促进体育发展的性别平等;以发展适应体育促进特殊群体的社会融入与健康。

关键词：以人民为中心体育主体性实践逻辑

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废弃油脂制备装配式建筑脱模剂的可行性分析

四川建材 2021年第2期47卷 123-124页

作者：胡敏颜子博彭丽郭娜四川建筑职业技术学院德阳市建材环保资源化工程技术研究中心四川德阳618000

装配式建筑施工过程中,预制预构件的制作和现场的混凝土的浇筑质量,除了混凝土施工的影响外,就是脱模剂的生产和应用水平。以铝模板和混凝土为主要对象,就废弃油脂制备装配式建筑用环保脱模剂进行可行性分析,可得:利用废弃油脂为原料,... 详细信息

装配式建筑施工过程中,预制预构件的制作和现场的混凝土的浇筑质量,除了混凝土施工的影响外,就是脱模剂的生产和应用水平。以铝模板和混凝土为主要对象,就废弃油脂制备装配式建筑用环保脱模剂进行可行性分析,可得:利用废弃油脂为原料,采用低碳环保且零废物生产工艺,制备新型环保脱模剂,适用于装配式建筑混凝土构配件的预制和现场浇筑,技术可行,成本低,效益高。

关键词：装配式施工资源化环保可行性