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Zoological Research 2020年第3期41卷 281-291页

作者： zi-qin jiang Han-Yu Wu Jing Tian Ning Li Xiao-Xiang Hu College of Biological Sciences China Agricultural UniversityBeijing 100094China State Key Laboratory of Agro-Biotechnology China Agricultural UniversityBeijing 100094China

Recent advances in avian transgenic studies highlight the possibility of utilizing lentiviral vectors as tools to generate transgenic chickens. However, low rates of gonadal chimerism and germ line transmission efficiency still limit the broad usage of this method in creating transgenic chickens. In this study, we implemented a simple strategy using modified lentiviral vectors targeted to chicken primordial germ cells(PGCs) to generate transgenic chickens. The lentiviral vectors were pseudotyped with a modified Sindbis virus envelope protein(termed M168) and conjugated with an antibody specific to PGC membrane proteins. We demonstrated that these optimized M168-pseudotyped lentiviral vectors conjugated with SSEA4 antibodies successfully targeted transduction of PGCs in vitro and in vivo. Compared with the control, 50.0%–66.7% of chicken embryos expressed green fluorescent protein(GFP) in gonads transduced by the M168-pseudotyped lentivirus. This improved the targeted transduction efficiency by 30.0%–46.7%. Efficient chimerism of exogenous genes was also observed. This targeting technology could improve the efficiency of germ line transmission and provide greater opportunities for transgenic poultry studies.

关键词： M168-pseudotyped lentiviral vectors Primordial germ cells Targeted transduction Transgenic chickens SSEA4

双撑杆索穹顶施工成形和静力性能试验研究（英文）

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2018年第3期19卷 225-239页

作者： Ai-lin ZHANG Chao SUN zi-qin jiang College of Architecture and Civil Engineering Beijing University of Technology Beijing Key Laboratory of Advanced Manufacturing Technology Beijing University of Technology Beijing Advanced Innovation Center for Future City Design Beijing University of Civil Engineering and Architecture

目的:提出一种稳定性好、便于张拉施工的双撑杆索穹顶结构,以解决该结构的找力分析问题,并考察其施工方法和静力性能,为工程实践提供参考依据。方法:1.通过节点平衡方程,推导出考虑结构自重时的双撑杆索穹顶结构的预应力分布计算公式;2... 详细信息

目的:提出一种稳定性好、便于张拉施工的双撑杆索穹顶结构,以解决该结构的找力分析问题,并考察其施工方法和静力性能,为工程实践提供参考依据。方法:1.通过节点平衡方程,推导出考虑结构自重时的双撑杆索穹顶结构的预应力分布计算公式;2.通过模型试验,比较采用空中组装和整体提升两种施工方法时,结构的内力和位形变化;3.进行满跨荷载和半跨荷载的加载测试,并与有限元分析结果进行了对比。结论:1.本文所得公式可以准确得到双撑杆索穹顶结构考虑自重后的实际预应力分布。2.该结构适合通过张拉外斜索施加预应力;结合整体提升的施工方法,可以显著降低施工难度,使其更适用于实际工程。3.在外荷载作用下,双撑杆索穹顶结构中脊索和内斜索的内力减小,其他斜索和环索的内力增大。

关键词：双撑杆索穹顶找力分析施工成形过程静力性能模型试验

内核外伸铰接防屈曲支撑设计理论研究.第二部分:数值验证（英文）

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2015年第10期16卷 793-804页

作者： zi-qin jiang Yan-lin GUO Jing-zhong TONG Xing YUAN College of Architecture and Civil Engineering Beijing University of Technology Department of Civil Engineering Tsinghua University

目的:本文旨在对系列文章第一部分所得到的理论推导结果进行相应的有限元数值验证,同时对内核单波变形下的支撑构件进行参数分析。方法:采用通用有限元软件ABAQUS建立六个防屈曲支撑模型对内核单波变形下理论推导公式进行校核;并对理论... 详细信息

目的:本文旨在对系列文章第一部分所得到的理论推导结果进行相应的有限元数值验证,同时对内核单波变形下的支撑构件进行参数分析。方法:采用通用有限元软件ABAQUS建立六个防屈曲支撑模型对内核单波变形下理论推导公式进行校核;并对理论公式进行参数分析,考察外围构件抗弯刚度、间隙大小、内核构件厚度、内核构件宽厚比、铰接接头长度、外围约束构件初始缺陷、外围构件长度、内核约束加强段长度及内核加强段翼板高度等9个参数对内核挤压力及外围构件受力影响;最后通过对12个防屈曲支撑进行数值模拟,验证支撑设计理论的合理性。结论:该理论设计公式能较好地预测支撑的破坏情况及内力发展情况,具有较强的安全性和较广的适用性,为铰接防屈曲支撑的使用推广奠定理论基础。

关键词：防屈曲支撑内核单波整体变形挤压力分布内核构件加强段设计准则精细有限元

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Experimental study of the pinned double rectangular tube assembled buckling-restrained brace

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2017年第1期18卷 20-32页

作者： zi-qin jiang Yan-lin GUO Ai-lin ZHANG Chao DOU Cai-xia ZHANG College of Architecture and Civil Engineering Beijing University of Technology Beijing 100124 China Department of Civil Engineering Tsinghua University Beijing 100084 China Beijing Engineering Research Center of High-rise and Large-span Prestressed Steel Structure Beijing University of Technology Beijing 100124 China School of Civil Engineering Beijing Jiaotong University Beijing 100044 China Beijing Key Laboratory of Advanced Manufacturing Technology Beijing University of Technology Beijing 100124 China

In this study, seven pinned double-rectangular tube assembled buckling-restrained brace （DRT-ABRB） specimens were experimentally charaeterised by means of an axial cyclic test. The core member of the specimens was a single flat-plate. Two rectangular tubes were assembled using high strength bolts to form an external restraining member. Each rectangular tube was composed of an external steel channel and a cover plate. A gap or thin rubber filler was set between the core and the external restraining member to form an unbonded layer. The influence of several design parameters on the failure mode and energy dissipation capacity of the ABRB was investigated, including the height of the core wing plate, thickness of the external cover plate, and height of the external channel flange. This experimental study demonstrated that a local pressure-bearing failure at the end of the external member arises when the external cover plate is too thin or if the end construction detail is unreasonable. When the end rotations of the DRT-ABRB were restricted, the hysteretic performance was shown to be superior to that of a pure pinned DRT-ABRB. Finally, all the tested DRT-ABRBs exhibited excellent energy dissipation performance which amply satisfied existing regulation requirements.

关键词： Double-rectangular tube assembled buckling-restrained brace (DRT-ABRB) Construction detail Hysteretic behaviour Failure mode High strength bolt

可恢复功能装配式梁柱中柱节点抗震性能研究（英文）

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2017年第12期18卷 927-941页

作者： Ai-lin ZHANG Ying-xia WU zi-qin jiang Xu-qiao ZHANG Chao DOU College of Architecture and Civil Engineering Beijing University of TechnologyBeijing 100124China China State Construction Engineering Corporation Beijing 100029China Beijing Engineering Research Center of High-rise and Large-span Prestressed Steel Structure Beijing 100124China School of Civil Engineering Beijing Jiaotong UniversityBeijing 100044China

目的:提出一种震后功能可快速恢复的装配式中柱节点,并建立其设计理论;考察装配式中柱节点的抗震性能及震后修复性能,并提出设计建议。创新点:提出一种可快速恢复震后功能的装配式中柱节点;2.提出装配式中柱节点设计理论,并通过数值手... 详细信息

目的:提出一种震后功能可快速恢复的装配式中柱节点,并建立其设计理论;考察装配式中柱节点的抗震性能及震后修复性能,并提出设计建议。创新点:提出一种可快速恢复震后功能的装配式中柱节点;2.提出装配式中柱节点设计理论,并通过数值手段对其合理性进行校核;3.试验研究考察基础试件的抗震性能及震后修复性能。方法:1.通过理论分析手段建立装配式中柱节点设计理论;2.利用数值模拟手段通过4组8个算例来验证节点设计理论,并对节点抗震性能进行数值研究;3.对基础试件进行低周往复荷载试验研究及修复试验研究,进一步验证设计理论的合理性,并考察节点的抗震性能及震后修复性能。结论:1.提出的设计理论可准确地预测装配式中柱节点的屈服荷载;2.设计合理的装配式中柱节点具有良好的承载性能及滞回特性,可满足结构震后功能修复要求;3.翼缘连接盖板厚度和材性、中间螺栓间距和梁段间隙参数对节点抗震性能影响较大,需合理设置。

关键词：可恢复功能翼缘盖板连接狗骨削弱设计理论滞回性能

The gut-eye axis:from brain neurodegenerative diseases to age-related macular degeneration

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Neural Regeneration Research 2025年第10期20卷 2741-2757页

作者： Qianzi Jin Suyu Wang Yujia Yao qin jiang Keran Li Department of Ophthalmology The Affiliated Eye Hospital of Nanjing Medical UniversityNanjingJiangsu ProvinceChina The Fourth School of Clinical Medicine Nanjing Medical UniversityNanjingJiangsu ProvinceChina

Age-related macular degeneration is a serious neurodegenerative disease of the retina that significantly impacts ***,the specific pathogenesis remains unclear,and effective early treatment options are consequently *** microbiome is defined as a large ecosystem of microorganisms living within and coexisting with a *** intestinal microbiome undergoes dynamic changes owing to age,diet,genetics,and other *** dysregulation of the intestinal flora can disrupt the microecological balance,resulting in immunological and metabolic dysfunction in the host,and affecting the development of many *** recent decades,significant evidence has indicated that the intestinal flora also influences systems outside of the digestive tract,including the ***,several studies have demonstrated the critical role of the gut-brain axis in the development of brain neurodegenerative diseases,including Alzheimer’s disease and Parkinson’s ***,the role of the“gut-eye axis”has been confirmed to play a role in the pathogenesis of many ocular ***,age-related macular degeneration and many brain neurodegenerative diseases have been shown to share several risk factors and to exhibit comparable *** such,the intestinal flora may play an important role in age-related macular *** the above context,the present review aims to clarify the gut-brain and gut-eye connections,assess the effect of intestinal flora and metabolites on age-related macular degeneration,and identify potential diagnostic markers and therapeutic ***,direct research on the role of intestinal flora in age-related macular degeneration is still relatively limited,while studies focusing solely on intestinal flora are insufficient to fully elucidate its functional role in age-related macular ***-on-a-chip technology has shown promise in clarifying the gut-eye interactions,while integrating analysis of the inte

关键词： age-related macular degeneration biological agents blinding eye disease dietary nutrition fecal microbial transplantation gut-eye axis intestinal flora metabolite microecology neurodegenerative disease

基于目标诱导银沉积的可视化检测方法的构建及其应用

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分析化学 2018年第8期46卷 1215-1221页

作者：字琴江王瑾曹秋娥周川华云南大学化学科学与工程学院自然资源药物化学教育部重点实验室

制备稳定且易于修饰的纳米金是构建基于金纳米颗粒可视化分析方法的关键。本研究以谷胱甘肽（GSH）为配体,分别采用一步法和两步法制备了两种纳米金颗粒,并对这两种纳米金的粒径、胶体稳定性等性能进行考察,发现两步法合成的纳米金胶体... 详细信息

制备稳定且易于修饰的纳米金是构建基于金纳米颗粒可视化分析方法的关键。本研究以谷胱甘肽（GSH）为配体,分别采用一步法和两步法制备了两种纳米金颗粒,并对这两种纳米金的粒径、胶体稳定性等性能进行考察,发现两步法合成的纳米金胶体稳定性更好。在此基础上,采用两步法合成的纳米金作为晶种和催化剂,建立了一种基于金属纳米颗粒的非聚集可视化检测抗坏血酸（AA）的方法。此方法结合了目标诱导银沉积反应和纳米银大的摩尔消光系数,利用AA在纳米金的存在下将Ag;还原成银单质所导致的检测液颜色变化及纳米银在410 nm处的吸光度变化,对AA进行定性以及定量检测,检出限为0.75μmol/L（S/N=3）。此方法具有简单、快速、灵敏、所需仪器设备简单、特异性好、抗干扰能力强等优点,可用于复杂样品中AA的高灵敏检测,在生物检测方面具有良好的应用前景。

关键词：纳米金抗坏血酸目标诱导银沉积可视化检测

Reduced mesencephalic astrocyte-derived neurotrophic factor expression by mutant androgen receptor contributes to neurodegeneration in a model of spinal and bulbar muscular atrophy pathology

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Neural Regeneration Research 2025年第9期20卷 2655-2666页

作者： Yiyang qin Wenzhen Zhu Tingting Guo Yiran Zhang Tingting Xing Peng Yin Shihua Li Xiao-jiang Li Su Yang Guangdong Key Laboratory of Non-human Primate Research Key Laboratory of CNS Regeneration(Ministry of Education)Guangdong–HongKong–Macao Institute of CNS RegenerationJinan UniversityGuangzhouGuangdong ProvinceChina

Spinal and bulbar muscular atrophy is a neurodegenerative disease caused by extended CAG trinucleotide repeats in the androgen receptor gene,which encodes a ligand-dependent transcription facto *** mutant androgen receptor protein,characterized by polyglutamine expansion,is prone to misfolding and forms aggregates in both the nucleus and cytoplasm in the brain in spinal and bulbar muscular atrophy *** aggregates alter protein-protein interactions and compromise transcriptional *** this study,we reported that in both cultured N2a cells and mouse brain,mutant androgen receptor with polyglutamine expansion causes reduced expression of mesencephalic astrocyte-de rived neurotrophic *** n of mesencephalic astrocyte-derived neurotrophic factor amelio rated the neurotoxicity of mutant androgen receptor through the inhibition of mutant androgen receptor *** down endogenous mesencephalic astrocyte-derived neurotrophic factor in the mouse brain exacerbated neuronal damage and mutant androgen receptor *** findings suggest that inhibition of mesencephalic astrocyte-derived neurotrophic factor expression by mutant androgen receptor is a potential mechanism underlying neurodegeneration in spinal and bulbar muscular atrophy.

关键词： androgen receptor mesencephalic astrocyte-derived neurotrophic factor mouse model neurodegeneration neuronal loss neurotrophic factor polyglutamine disease protein misfolding spinal and bulbar muscular atrophy transcription factor

Non-coding RNAs in acute ischemic stroke:from brain to periphery

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Neural Regeneration Research 2025年第1期20卷 116-129页

作者： Shuo Li Zhaohan Xu Shiyao Zhang Huiling Sun Xiaodan qin Lin Zhu Teng jiang Junshan Zhou Fuling Yan Qiwen Deng Department of Neurology Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu ProvinceChina Department of Neurology Zhongda HospitalSchool of MedicineSoutheast UniversityNanjingJiangsu ProvinceChina General Clinical Research Center Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu ProvinceChina

Acute ischemic stroke is a clinical emergency and a condition with high morbidity,mortality,and *** predictive,diagnostic,and prognostic biomarkers and effective therapeutic targets for acute ischemic stroke remain *** innovations in high-throughput gene sequencing analysis,many aberrantly expressed non-coding RNAs(ncRNAs)in the brain and peripheral blood after acute ischemic stroke have been found in clinical samples and experimental *** expressed ncRNAs in the post-stroke brain were demonstrated to play vital roles in pathological processes,leading to neuroprotection or deterioration,thus ncRNAs can serve as therapeutic targets in acute ischemic ***,distinctly expressed ncRNAs in the peripheral blood can be used as biomarkers for acute ischemic stroke prediction,diagnosis,and *** particular,ncRNAs in peripheral immune cells were recently shown to be involved in the peripheral and brain immune response after acute ischemic *** this review,we consolidate the latest progress of research into the roles of ncRNAs(microRNAs,long ncRNAs,and circular RNAs)in the pathological processes of acute ischemic stroke–induced brain damage,as well as the potential of these ncRNAs to act as biomarkers for acute ischemic stroke prediction,diagnosis,and *** from this review will provide novel ideas for the clinical application of ncRNAs in acute ischemic stroke.

关键词： acute ischemic stroke apoptosis blood–brain barrier damage circular RNAs excitatory toxicity long non-coding RNAs microRNAs neuroinflammation non-coding RNAs oxidative stress

Clinicopathological differences between patients with schistosomal appendicitis and non schistosomal appendicitis:A retrospectively study of past ten years

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World Journal of Clinical Cases 2025年第2期13卷 24-32页

作者： Xiao-Yi Wang Yao Hao zi-Jian Wang Xiu-Liang Xu jiang-Hua Yang Department of Infectious Diseases The First Affiliated of Wannan Medical CollegeWuhu 241001Anhui ProvinceChina

BACKGROUND Chronic schistosomiasis causes multiple organ and multiple system diseases,especially the digestive *** eggs are mainly deposited in the stomach,liver and colorectal,but a few eggs are deposited in the appendix and cause *** present,there are few studies on schistosomal *** To explore the differences in epidemiological,clinical and pathological characteristics between schistosomal appendicitis and non-schistosomal appendicitis over the past decade in order to assess the impact of schistosomiasis on *** The differences of general data,clinical data and laboratory examination data of patients with appendicitis from October 2013 to October 2023 were retrospectively *** patients were divided into two groups for *** were 136 patients in schistosomal appendicitis group and 5418 patients in non-schistosomal appendicitis *** Schistosomal appendicitis accounted for 2.45%of all patients with appendicitis,and the annual proportion in the past decade was 2.2%,2.9%,1.8%,1.9%,3.4%,3.1%,1.9%,1.6%,3%,2.6%,*** prevalence of schistosomal appendicitis was middle-aged and elderly males,with an average age of 61.73±15.335 *** main population of non-schistosomal appendicitis was middle-aged men,with an average age of 35.8±24.013 years(P<0.001).The distribution of pathological types of appendicitis was different between the two groups(P<0.001).The incidence of acute suppurative appendicitis in non-schistosomal appendicitis was higher than that in schistosomal appendicitis[odds ratio(OR)=0.504;95%confidence interval(CI):0.349-0.728;P<0.001].The proportion of acute attack of chronic appendicitis in schistosomal appendicitis was higher than that in non-schistosomal appendicitis(OR=2.614;95%CI:1.815-3.763;P<0.001).The proportion of schistosomal appendicitis patients complicated with colorectal cancer was higher than that of nonschistosomal appendicitis patients(OR=5.087;95%CI:1.427-

关键词： Schistosomal appendicitis Schistosomiasis Appendix Colorectal cancer Clinicopathological characteristics