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Development of indirect competitive fluorescence immunoassay for 2,2′,4,4′-tetrabromodiphenyl ether using DNA/dye conjugate as antibody multiple labels

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Journal of Environmental Sciences 2012年第7期24卷 1334-1340页

作者： zi-yan fan Young Soo Keum Qing-Xiao Li Weilin L.Shelver Liang-Hong Guo State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-environment SciencesChinese Academy of SciencesBeijing 100085China College of Life and Environmental Sciences Konkuk University in Korea1 Hwayang-dongGwangjin-guSeoul 143-701Korea Department of Molecular Bioscience and Bioengineering University of Hawaii at Manoa1955 East-West RoadHonoluluHI 96822USA USDA-ARS Bioscience Research Laboratory 1605 Albrechht BoulevardFargoND 58105USA

An indirect competitive fluorescence immunoassay using a DNA/dye conjugate as antibody multiple labels was developed on 96-well plates for the identification and quantification of 2,2＇,4,4＇-tetrabromodiphenyl ether （BDE-47） in aqueous samples. A hapten, 2,4,2＇- tribromodiphenyl ether-4＇-aldehyde, was synthesized, and was conjugated to bovine serum albumin to form a coating antigen. Specific recognition of the antigen by anti-PBDE antiserum was confirmed by a surface plasmon resonance measurement. In the immunoassay, the coating antigen was adsorbed on a 96-well plate first, and a sample, antiserum and biotinylated goat anti-rabbit secondary antibody were then added and reacted sequentially. A biotinylated, double-stranded DNA with 219 base pairs was attached to the secondary antibody by using streptavidin as a molecular bridge. In situ multiple labeling of the antibody was accomplished after addition of a DNA-binding fluorescent dye, SYBR Green I. The working range of the immunoassay for the BDE-47 standard was 3.1-390 ~tg/L, with an IC50 value of 15.6 Ixg/L. The calculated LOD of the immunoassay is 0.73 Ixg/L. The immunoassay demonstrated relatively high selectivity for BDE-47, showing very low cross-reactivity （〈 3%） with BDE-15, BDE-153 and BDE-209. With a spiked river water sample containing 50 Izg/L BDE-47, quantification by the immunoassay was 41.9 ~tg/L, which compared well with the standard GC-ECD method （45.7 Ixg/L）. The developed immunoassay provides a rapid screening tool for polybrominated diphenyl ethers in environmental samples.

关键词： polybrominated diphenyl ethers fluorescence immunoassay microplate multiple labeling

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Research,application and development of inorganic binder for casting process

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China Foundry 2024年第5期21卷 461-475页

作者： Xiao-long Gong Sheng-li Hu zi-tian fan State Key Laboratory of Materials Processing and Die&Mould Technology School of Materials Science and EngineeringHuazhong University of Science and TechnologyWuhan 430074China School of Materials Science and Engineering Wuhan University of TechnologyWuhan 430070China

Inorganic binder used in casting process has the advantages of low odor,labor-friendly conditions,and relatively low cost,which is one of the main development directions for casting molding materials in the ***,compared to organic binders(such as resin binders),inorganic binders exhibit lower bonding strength and are more sensitive to environmental *** sensitivity poses challenges,particularly in the reclamation of used sand,thus limiting their broader *** this paper,the research and application status of inorganic binders(mainly silicate inorganic binders)and their curing methods are *** addition,the research and application of phosphate inorganic binders and 3D printing inorganic binders that are being developed are ***,a detailed comparative analysis is conducted on the challenging issue of“reclamation for used sand”in the application of inorganic ***,the development direction of inorganic binders is clarified.

关键词： inorganic binder silicate system phosphate system 3D printing used sand reclamation development trend

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The New Methods for Characterization of Molecular Weight of Supramolecular Polymers

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Chinese Journal of Polymer Science 2024年第10期42卷 1403-1413,I0006页

作者： Hui Liu Rui Hu zi-Qing Hu Xiao-fan Ji Key Laboratory of Materials Chemistry for Energy Conversion and Storage Ministry of EducationHubei Key Laboratory of Materials Chemistry and Service FailureHubei Engineering Research Center for Biomaterials and Medical Protective MaterialsSchool of Chemistry and Chemical EngineeringHuazhong University of Science and TechnologyWuhan430074China

Supramolecular polymers,as a type of dynamic polymers,are subordinate to the interdisciplinary field of polymer chemistry and supramolecular chemistry,whose development has greatly promoted the prosperity of new ***,molecular weight is one of the most important parameters of supramolecular polymers,which affects the physical/chemical properties and processing applications of *** new methods for characterizing the molecular weight of supramolecular polymers is crucial for advancing the development of supramolecular *** this review,we elaborate and summarize three strategies for characterizing the molecular weight of supramolecular polymers that recently reported by our research group according to the characteristics of supramolecular polymers,including(1)the molecular weight distinction corresponding to variable fluorescence colors,(2)matching different molecular weights with different fluorescence lifetime,(3)transforming supramolecular polymers into mechanically interlocked polymers or covalent ***,we also discuss the limitations of current methods for characterizing supramolecular *** hope that this review can promote the development of supramolecular polymers and significantly inspire to exploit new methods to characterizing molecular weight of supramolecular polymers.

关键词： Supramolecular polymers Molecular weight Self-assembly Mechanically interlocked polymers Dynamic polymers

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Recent advances in promising drugs for primary prevention of gastroesophageal variceal bleeding with cirrhotic portal hypertension

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Hepatobiliary & Pancreatic Diseases International 2024年第1期23卷 4-13页

作者： Ji-Yao Sheng zi-fan Meng Qiao Li Yong-Sheng yang Department of Hepatobiliary and Pancreatic Surgery the Second Hospital of Jilin UniversityChangchun 130041China Jilin Engineering Laboratory for Translational Medicine of Hepatobiliary and Pancreatic Diseases the Second Hospital of Jilin UniversityChangchun 130041China

Background:Gastroesophageal variceal bleeding is one of the most severe complications of patients with *** primary prevention drugs,including non-selectiveβ-blockers,have effectively reduced the incidence of bleeding,their efficacy is limited due to side effects and related *** recent advances in precision medicine,precise drug treatment provides better treatment *** sources:Literature search was conducted in PubMed,MEDLINE and Web of Science for relevant articles published up to May *** on clinical trials was obtained from https://***/and http://***/.Results:The in-depth understanding of the pathogenesis and advances of portal hypertension has enabled the discovery of multiple molecular targets for promising *** to the site of action,these drugs could be classified into four classes:intrahepatic,extrahepatic,both intrahepatic and extrahepatic targets and *** these classes of drugs offer advantages over traditional treatments in prevention of gastroesophageal variceal bleeding in patients with cirrhotic portal ***:This review classified and summarized the promising drugs,which prevent gastroesophageal variceal bleeding by targeting specific markers of pathogenesis of portal hypertension,demonstrating the significance of using the precision medicine strategy to discover and develop promising drugs for the primary prevention of gastroesophageal variceal bleeding in patients with cirrhotic portal hypertension.

关键词： Cirrhotic portal hypertension Target drug Primary prevention Bleeding

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Application of immune checkpoint inhibitors and microsatellite instability in gastric cancer

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World Journal of Gastroenterology 2024年第21期30卷 2734-2739页

作者： Shi-yan yan Jian-Gao fan Department of Gastroenterology Xinhua HospitalShanghai Jiaotong University School of MedicineShanghai 200092China

In this editorial we comment on the article by Li published in the recent issue of the World Journal of *** focus specifically on the application of immune checkpoint inhibitors(ICIs)and microsatellite instability(MSI)in gastric cancer(GC).The four pillars of GC management have long been considered,including surgery,chemotherapy,radiotherapy and targeted ***,immunotherapy has recently emerged as a“fifth pillar”,and its use is rapidly *** are four principal strategies for tumor immunotherapy:ICIs,tumor vaccines,adoptive immunotherapy and nonspecific *** them,ICIs are the most advanced and widespread type of cancer immunotherapy for *** breakthrough results for ICIs have paved the way to a new era of cancer *** particular,inhibition of the PD-1/PD-L1 axis with ICIs,including nivolumab and pembrolizumab,has emerged as a novel treatment strategy for advanced ***,these therapies are sometimes associated with often subtle,potentially fatal immune-related adverse events(irAEs),including dermatitis,diarrhea,colitis,endocrinopathy,hepatotoxicity,neuropathy and *** must be aware of these irAEs and improve the detection of these processes to prevent inappropriate discharges,emergency department revisits,and downstream *** studies have revealed that MSI-high or mismatch-repair-deficient tumors,regardless of their primary site,have a promising response to ***,it is important to detect MSI before applying ICIs for treatment of GC.

关键词： Gastric cancer Immune checkpoint inhibitors Microsatellite instability Immunotherapy Immune-related adverse events

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Climatic implications in earlywood and latewood width indices of Chinese pine in north central China

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Journal of Forestry Research 2024年第3期35卷 143-153页

作者： Kaixuan yang Junzhou Zhang Haowen fan Yuan yan College of Geographic Sciences Qinghai Normal UniversityXining 810016People's Republic of China Key Laboratory of Tibetan Plateau Land Surface Processes and Ecological Conservation(Ministry of Education) Xining 810016People's Republic of China Key Laboratory of Western China's Environmental Systems(Ministry of Education) College of Earth and Environmental SciencesLanzhou UniversityLanzhou 730030People's Republic of China Gansu Liancheng Forest Ecosystem Field Observation and Research Station Lanzhou UniversityLanzhou 730333People's Republic of China School of Geography and Information Engineering University of GeoscienceWuhan 430074People's Republic of China

Latewood width(LWW)indices of trees are considered a reliable proxy of summer precipitation in the Northern ***,the strong coupling and high correlation between earlywood width(EWW)and LWW indices often prevent registration of climate signals of the LWW *** this study,328-year-long earlywood width and latewood width chronologies were developed from Chinese pine at two sites in the Hasi Mountains,north central *** climate responses of these chronologies were analyzed and the LWW index used to derive sum-mer precipitation *** analyses showed that LWW was particularly influenced by earlywood growth and recorded stronger climate signals of the previous year as EWW,rather than those of the current year with infrequent summer climate ***,after removing the effect of earlywood growth using a simple regression model,the adjusted LWW chronology(LWW_(adj))showed a strong relationship with July precipitation in dry *** suggests that the LWW_(adj) chronology has the potential to be used to investigate long-term variability in summer precipitation in drought-limited regions.

关键词： Dendroclimatology Latewood width Climate response Summer precipitation Earlywood effect

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Bioinspired designs in active metal-based batteries

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Nano Research 2024年第2期17卷 587-601页

作者： fan Zhang Ting Liao Cheng yan ziqi Sun School of Chemistry and Physics Queensland University of TechnologyGeorge StreetBrisbaneQLD 4000Australia School of Mechanical Medical and Process Engineering Queensland University of TechnologyGeorge StreetBrisbaneQLD 4000Australia QUT Centre for Materials Science Queensland University of TechnologyGeorge StreetBrisbaneQLD 4000Australia

Active metal-based batteries are drawing increased attention because of their inherent high energy density and specific *** grand challenges,such as dendrite growth,electrode degradation,rapid performance fading,etc.,have limited their practical ***,which involves taking cues from the structures and functions of the natural world,can lead to a wealth of conceptually fresh approaches to regulator the metal ion transportation to achieve a dendrite-free metal plating,thwart the side-reaction reactions,and retard the structural distortions,for a more reliable and secure operation of active metal-based *** this review,we concentrate on the fabrication and application of bioinspired designs in active metal-based batteries with enhanced performance,along with discussion on the challenges and opportunities associated with this promising *** anticipate that this review can offer some insights into the development of functional materials by learning from nature and provide some approaches for the innovations of either the battery structures or the energy materials for metal-based batteries.

关键词： bioinspired materials energy storage devices metal-based batteries

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Effects of formal credit on pastoral household expense: Evidence from the Qinghai-Xizang Plateau of China

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Journal of Integrative Agriculture 2024年第5期23卷 1774-1785页

作者： yan Zhang Yi Huang fan Zhang Zeng Tang State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems College of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhou 730020China

Formal credit is critical in agricultural production,allowing more expenditure and productive input,thereby improving farmers'*** pastoral China,formal financial institutions are gradually ***,a limited understanding remains of how formal credit affects herders'household *** on a survey of 544 herders from the Qinghai-Xizang Plateau of China,this study adopted the propensity score matching approach to identify the effect of formal credit on herders'total household expenses,daily expenses,and productive *** results found that average age,grassland mortgage,and other variables significantly affected herders'participation in formal *** credit could significantly improve household expenses,especially productive expenses.A heterogeneity analysis showed that formal credit had a greater impact on the household total expense for those at higher levels of wealth;however,it significantly affected the productive expense of herders at lower wealth ***,the mediating effect indicated that formal credit could affect herders'household income,thus influencing their household ***,this study suggests that policies should improve herders'accessibility to formal credit.

关键词： formal credit herders expense Qinghai-Xizang Plateau

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Observation of an upward lightning flash with 21 upward positive leaders initiated from different wind turbines in wind farm

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High Voltage 2024年第1期9卷 163-171页

作者： Li Cai Yifeng Ke Wenchao fan Ruisheng yan Mi Zhou Jianguo Wang Yadong fan Engineering Research Center of Ministry of Education for Lightning Protection and Grounding Technology School of Electrical Engineering and AutomationWuhan UniversityWuhanChina

As the wind turbine height increases,the probability of multiple upward leaders flashes within the wind farm *** this study,an upward lightning flash containing 21 upward positive leaders(UPLs)initiated from different wind turbines in Zhangbei Wind Farm was *** average speed of the UPLs was 1.2×10^(5) m/s,which mostly decreased to zero before reaching the cloud *** four UPLs developed to cloud height,with prolonged periods of bright light on three of them and with a minimum duration of 126 ms.A total of nine return strokes occurred on the other two dissipated leader channels,and the numbers of strokes on the two channels was six and three,*** average interstroke interval for the return strokes was 10.5 *** of these nine return strokes had peak currents smaller than 10 *** high-speed video frames confirmed that competition does exist between the UPLs,and the range of shielding effects increases as the height of the ULs *** effect of fan rotation on the initiation of upward leaders and the shielding effect between the wind turbines and upward leaders are discussed.

关键词： flash return initiated

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Experimental and numerical analyses of the effect of fibre content on the close-in blast performance of a UHPFRC beam

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Defence Technology（防务技术） 2024年第1期31卷 242-261页

作者： Junbo yan Qiyue Zhang yan Liu Yingliang Xu Zhenqing Shi fan Bai Fenglei Huang State Key Laboratory of Explosion Science and Technology Beijing Institute of TechnologyBeijing 100081China Beijing Institute of Technology Chongqing Innovation Center Chongqing 401120China

Limited research has been conducted on the influences of fiber content on close-in blasting characteristics for ultrahigh-performance fiber-reinforced concrete(UHPFRC)*** paper aims to address this knowledge gap through experimental and mesoscale numerical *** were conducted on ten UHPFRC beams built with varying steel fiber volumetric fractions subjected to close-in explosive ***,this study considered other parameters,such as the longitudinal reinforcement type and *** the case of UHPFRC beams featuring normal-strength longitudinal reinforcement of diametersΦ12,Φ16,andΦ20,a reduction in maximum displacement by magnitudes of19.6%,19.5%,and 17.4%was observed,respectively,as the volumetric fractions of fiber increased from1.0%to 2.5%.In addition,increasing the longitudinal reinforcement ratio and using high-strength steel longitudinal reinforcement both significantly reduced the deformation characteristics and increase the blasting resistances of UHPFRC ***,the effects on the local crushing and spalling damage were not significant.A mesoscale finite element model,which considers the impacts of fiber parameters on UHPFRC beam behaviors,was also established and well correlated with the test ***,parametric analyses were further conducted to examine the impacts of the steel fiber content and length and the hybrid effects of various types of microfibers and steel fibers on the blasting performance of UHPFRC beams.

关键词： Blast performance Close-in blast Fiber content Mesoscale approach UHPFRC beams

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