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Comparative chloroplast genome analysis of medicinally important Veratrum(Melanthiaceae)in China:Insights into genomic characterization and phylogenetic relationships

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Plant Diversity 2022年第1期44卷 70-82页

作者： Ying-Min zhang Li-jun Han Cong-Wei Yang zi-Li Yin Xing Tian zi-Gang Qan Guo-Dong Li Faculty of Traditional Chinese Pharmacy Yunnan University of Chinese MedicineKunming 650500YunnanChina Yunnan Key Laboratory for Dai and Yi Medicines Yunnan University of Chinese MedicineKunming 650500YunnanChina

Members of Veratrum are perennial herbs widely used in traditional Chinese medicine to induce vomiting,resolve blood stasis and relieve ***,the intrageneric classification and phylogenetic relationships within Veratrum have long been controversial due to the complexity of morphological variations and lack of high-resolution molecular *** this study,we reevaluated the infrageneric relationships with the genus Veratrum using complete chloroplast genome sequence ***,the complete cp genomes of ten species of Veratrum were newly sequenced and *** complete cp genomes of ten species of Veratrum had the typical quadripartite structure,ranging from 151,597 bp to 153,711 bp in size and comprising a total of 135 *** structure of Veratrum cp genomes(i.e.,gene order,content,and genome components)was highly similar across *** number of simple sequence repeats(SSRs)ranged from 63 to 7&and of long repeats ranged from 31 to *** highly divergent regions(ndhF,psbC-psbZ,psbK-psbl,rpoB-tmCjGCA,tmK_UUU-tmQ_UUG,tmS_GCU-tmG_UCC,tmT_UGU-tmL_UAA and ycfl)were identified and are potentially useful for the DNA barcoding of *** analysis among 29 taxa based on cp genomes,total genes,protein-coding genes and intergenic regions strongly supported the monophyly of *** circumscription and relationships of the infrageneric taxa of Veratrum were well-presented with great *** results will facilitate the identification,taxonomy,and utilization of Veratrum plants as well as the evolutionary studies of Melanthiaceae.

关键词： Veratrum Melanthiaceae Chloroplast genome Comparative analysis Phylogeny

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Bumblebees with the socially transmitted microbiome:A novel model organism for gut microbiota research

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Insect Science 2022年第4期29卷 958-976页

作者： zi-Jing zhang Hao Zheng College of Food Science and Nutritional Engineering China Agricultural UniversityBeijingChina

Eusocial bumble and honey bees are important pollinators for global ecology and the agricultural *** both the bumble and honey bees possess similar and host-restricted gut microbiota,they differ in aspects of morphology,autonomy,physiology,behavior,and life *** social bee gut bacteria exhibit host specificity that is likely a result of long-term *** unique life cycle of bumblebees is key for the acquisition and development of their gut microbiota,and affects the strain-level diversity of the core bacterial *** on bumblebee gut bacteria show that they retain less functional capacity for carbohydrate metabolism compared with that of the *** discuss the potential roles of the bumblebee gut microbiota against pathogenic threats and the application of host-specific probiotics for *** the advantages of the bumblebee microbiome,including the simple structure and host specificity,and the ease of manipulating bumblebee colonies,we propose that bumblebees may provide a valuable system for understanding the general principles of host-microbe interactions,gut-brain axis,and vertical transmission.

关键词： bumblebee gut microbiota host-microbe interaction model organism

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Numerical Modeling of the Compression Process of Elastic Open-cell Foams

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Chinese Journal of Aeronautics 2007年第3期20卷 215-222页

作者： zhang Jia-lei LU zi-xing Institute of Solid Mechanics Beijing University of Aeronautics and Astronautics Beijing 100083 China

The random models of open-cell foams that can reflect the actual cell geometrical properties are constructed with the Voronoi technique. The compression process of elastic open-cell foams is simulated with the nonlinear calculation module of finite element analysis program. In order to get the general results applicable to this kind of materials, the dimensionless compressive stress is used and the stress-strain curves of foam models with different geometrical properties are obtained. Then, the influences of open-cell geometrical properties, including the shape of strut cross section, relative density and cell shape irregularity, on the compressive nonlinear mechani- cal performance are analyzed. In addition, the numerical results are compared with the predicted results of cubic staggering model. Nu- merical results indicate that the simulated results reflect the compressive process of foams quite well and the geometrical properties of cell have significant influences on the nonlinear mechanical behavior of foams.

关键词： Voronoi technique open-cell foam nonlinear compressive stress

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3D graphical visualization of the genetic architectures underlying complex traits in multiple environments

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2007年第4期8卷 563-567页

作者： HU Cheng-cheng YE Xiu-zi zhang Yin YU Rong-dong YANG Jian ZHU jun College of Computer Science Zhejiang University Hangzhou 310027 China James D. Watson Institute of Genomic Science Zhejiang University Hangzhou 310029 China Institute of Bioinformatics. Zheiiane University. Hangzhou 310029. China

An approach for generating interactive 3D graphical visualization of the genetic architectures of complex traits in multiple environments is described. 3D graphical visualization is utilized for making improvements on traditional plots in quan- titative trait locus (QTL) mapping analysis. Interactive 3D graphical visualization for abstract expression of QTL, epistasis and their environmental interactions for experimental populations was developed in framework of user-friendly software QTLNetwork (http://***/software/qtlnetwork). Novel definition of graphical meta system and computation of virtual coordinates are used to achieve explicit but meaningful visualization. Interactive 3D graphical visualization for QTL analysis provides geneticists and breeders a powerful and easy-to-use tool to analyze and publish their research results.

关键词： Ouantitative trait locus (QTL) Visualization 3D graphical Interactive visualization

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Regular penis-root masturbation,a novel behavioral therapy in the treatment of primary premature ejaculation

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Asian Journal of Andrology 2019年第6期21卷 631-634页

作者： Gong-Chao Ma zi-jun Zou Yu-Fen Lai Xun zhang Yan zhang Departme nt of In fertility and Sexual Medicine The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510630China Department of Andrology The First Affiliated Hospital of Guangxi Medical UniversityNanning 530021China.

The primary premature ejaculation(PPE)is a common male sexual *** proposed a novel behavioral therapy for PPE through regular penis-root masturbation(PRM).Nine heterosexual men with PPE completed the self-controlled *** a 3-month PRM training,the median intravaginal ejaculatory latency time(IELT)increased from 60sto 180 s(P=0.018),and the mean Premature Ejaculation Diagnostic Tool(PEDT)score decreased from 14.8±3.7 to 12.8±4.1(P=0.074).Five out of eight patients had the prolonged dorsal nerve somatosensory evoked potential(DNSEP).Ihe results suggest that PRM has a short-term therapeutic *** controlled trials are needed to validate the efficacy.

关键词： evoked effect. Regular

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Side population cells from HXO-Rb44 retinoblastoma cell line have cancer-initiating property

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International Journal of Ophthalmology(English edition) 2011年第5期4卷 461-465页

作者： jun-jun She, zi-Ming Wang Department of General Surgery First Affiliated Hospital of Medical College Xi'an Jiaotong University Xi'an 710061 Shaanxi Province China Department of Mathematics Xidian University Xi'an 710071 Shaanxi Province China Department of Urology Second Affiliated Hospital of Medical College Xi'an Jiaotong University Xi'an 710004 Shaanxi Province China

AIM: To ascertain whether side population (SP) cells in HXO-Rb44 retinoblastoma cell line have cancer stem cell-like property in vitro and in vivo. METHODS: We analyzed and sorted SP from HXO-Rb44 retinoblastoma cell line by Hoechst 33342 staining on flow cytometry. SP and NSP cells were determined their ability of proliferation and self-renewal by SP reanalysis, soft agar assay and tumor sphere assay in vitro. Clone formation was detected by seeding HXO-Rb44 and HXO-Rb44 -RFP cells into soft agar. The expression of ABCGZ MDRI Bmi-1 and Oct-4 was determined by RT-PCR between SP and non-SP (NSP) cells. Moreover, they were injected into nude mice to determine their tumorigency in vivo. RESULTS: SP from HXO-Rb44 retinoblastoma cell line could grow clonally in soft agar assays and form tumor spheres from single cells in conditioned media. The expressions of ABCG2, MDRI Bmi-1 and Oct-4 were significantly higher in SP than NSP cells. As few as SP cells resulted in tumor formation in 6 of 12 injected sites, however, the injection of NSP cells failed to form new tumor. CONCLUSION: SP cells isolated by Hoechst 33342 from the HXO-Rb44 retinoblastoma cell line had property of high tumorigency in vivo and in vitro Therefore, SP might be a target while developing retinoblastoma therapies.

关键词： side population self-renewal retinoblastoma HXO-Rb44

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Effect of AI_(2)O_(3)and MgO on crystallization and structure of Ca0-Si0_(2)-B_(2)0_(3)-based fluorine-free mold flux

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Journal of Iron and Steel Research International 2021年第5期28卷 552-562页

作者： jun-yu Chen Wan-lin Wang Le-jun Zhou zi-hang Pan School of Metallurgy and Environment Central South UniversityChangsha 410083HunanChina National Center for International Cooperation of Clean Metallurgy Changsha 41(K)83HunanChina

The crytallization behavior and melt structure of the CaO-Si0^(2-)B20_(3)-based fluorine-free mold fux were *** results show that the crytallization of the mold fux was first inhibited and then promoted with the increase in Al_(2)0_(3) content from 4 to 12 wt.%.However,it was enhanced by MgO in the range of 2-10 wt.%.The results of Fourier transform infrared spectroscopy and Raman spectroscopy showed that Al_(2)0_(3) worked as a network former in the mold flux melt when its content was in the range of 4-8 wt.%,whereas it worked as the network breaker to provide 0^(2-)when its content was in the range of 8-12 wt.%.In addition,the combined efects from the charge compensation by Mg^(2+)and the network broken by 0^(2-)led to the increase in some typicalT-O-T(AI-O-A1,B-O-B,etc.,)and simpler structural units(Q^(2)(Si),B_^(O-)in the[B0_(2)0^(-)],.etc.)when the MgO content was in the range of 2-6 wt.%.The continuous increase in 0^(2-)provided by the addition of MgO from 6 to 10 wt.%further depolymerized the network of the melt and fnally caused fast crystallizationo.

关键词： Crystallization Single hot thermocouple technique Melt structure Fluorine-free mold flux

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Bayesian inference of the crust–core transition density via the neutron-star radius and neutron-skin thickness data

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Nuclear Science and Techniques 2023年第6期34卷 125-133页

作者： Wen-Jie Xie zi-Wei Ma jun-Hua Guo Department of Physics Yuncheng UniversityYuncheng044000China

In this work,we perform a Bayesian inference of the crust-core transition density ρ_(t) of neutron stars based on the neutron-star radius and neutron-skin thickness data using a thermodynamical *** and Gaussian distributions for the ρ_(t) prior were adopted in the Bayesian *** has a larger probability of having values higher than 0.1 fm^(−3) for ρ_(t) as the uniform prior and neutron-star radius data were *** was found to be controlled by the curvature K_(sym) of the nuclear symmetry *** phenomenon did not occur if K_(sym) was not extremely negative,namely,K_(sym)>−200 *** value ofρ_(t) obtained was 0.075_(−0.01)^(+0.005) fm^(−3) at a confidence level of 68%when both the neutron-star radius and neutron-skin thickness data were *** anti-correlations were observed between ρ_(t),slope L,and curvature of the nuclear symmetry *** dependence of the three L-K_(sym) correlations predicted in the literature on crust-core density and pressure was quantitatively *** most probable value of 0.08 fm^(−3) for ρ_(t) was obtained from the L-K_(sym) relationship proposed by Holt et *** larger values were preferred for the other two relationships.

关键词： Crust–core transition density of neutron stars Neutron-star radius Neutron-skin thickness Bayesian inference approach L–K_(sym)

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A mini-review on recent progress of new sensitizers for luminescence of lanthanide doped nanomaterials

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Nano Research 2020年第7期13卷 1795-1809页

作者： Hongxin zhang zi-Han Chen Xuan Liu Fan zhang Department of Chemistry Shanghai Key Laboratory of Molecular Catalysis and Innovative MaterialsState Key Laboratory of Molecular Engineering of Polymers and iChemFudan UniversityShanghai 200433China

Trivalent lanthanide(Ln2+)doped luminescent nanocrystals are promising for applications ranging from biosensor,lasing,super-resolution nanoscopy,information security and so *** the utility prospect is of great attractions,the light absorption of these lanthanide doped nanocrystals is inherently weak due to the electric dipole-forbidden 4f→4f *** worse,the quantum yields of upconverison nanocrystals are very low,which will unavoidably hinder their further *** a typical lanthanide luminescent nanosystem,both sensitizers as light absorption centers and activators as light emitting centers are necessary and important for desired luminescence *** various sensitization systems,only Yb3+and Nd+are considered as the most efficient ***,the corresponding excitation wavelengths are strictly limited around 980 and 808 *** enrich excitation wavelengths and boost luminescence intensity,exploring more sensitization units that possess larger absorption cross section,higher efficiency of energy transfer process and independent excitation is imperative and beneficial for the demands of different applications,such as broadened absorption in near infrared(NIR)region for higher conversion efficiency of solar cells,prolonged excitation wavelength to second near infrared windows region(NIR Ⅱ,1,000-1,700 nm)for in vivo fluorescence imaging with deeper tissue depth and higher spatial resolution,more orthogonal excitations and emissions to improve optical multiplexing,and so ***,in the review,we primarily conclude several major energy transfer mechanisms from sensitizers to *** we present three kinds of sensitizers,including lanthanide ions,organic dyes and quantum dots(QDs),and introduce the newly designed sensitization system that allows us to exploit superior excitation wavelength and amplity luminescence ***,several future challenges and opportunities for the sensitizing strategies are discuss

关键词： lanthanide luminescenee new sensitizers organic dyes quantum dots

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Successful individualized endodontic treatment of severely curved root canals in a mandibular second molar:A case report

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World Journal of Clinical Cases 2022年第14期10卷 4632-4639页

作者： Lai-jun Xu Jian-Ying zhang zi-Hua Huang Xiang-Zhu Wang Hunan Key Laboratory of Oral Health Research Hunan 3D Printing Engineering Research Center of Oral CareHunan Clinical Research Center of Oral Major Diseases and Oral HealthAcademician Workstation for Oral-maxilofacial and Regenerative Medicine and Xiangya Stomatological HospitalXiangya School of StomatologyCentral South UniversityChangsha 410008Hunan ProvinceChina

BACKGROUND The incidence rate of severely curved root canals in mandibular molars is low,and the root canal treatment of mandibular molars with this aberrant canal anatomy may be technically *** SUMMARY A 26-year-old Chinese female patient presented with intermittent and occlusal pain in the left mandibular second *** patient had undergone filling restoration for caries before endodontic *** the aid of cone beam computed tomography(CBCT),a large periapical radiolucency was observed,and curved root canals in a mandibular second molar were confirmed,depicting a severe and curved distolingual *** treatments,including novel individualized preparation skills and techniques and the use of bioceramic materials as an apical barrier,were performed,and complete healing of the periapical lesion and a satisfactory effect were *** A case of severely curved root canals in a mandibular second molar was successfully treated and are reported *** complex anatomy of the tooth and the postoperative effect were also evaluated via the three-dimensional reconstruction of CBCT images,which accurately identified the aberrant canal *** devices and biomaterial applications combined with novel synthesis techniques can increase the success rate of intractable endodontic treatment.

关键词： Cone beam computed tomography Canal curvature Mandibular second molar Root canal therapy Case report

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