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The MADS-box gene FveSEP3 plays essential roles in flower organogenesis and fruit development in woodland strawberry

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Horticulture Research 2021年第1期8卷 3533-3547页

作者： Mengting Pi Shaoqiang Hu Laichao Cheng Ruhan Zhong zhuoying cai Zhongchi Liu Jia-Long Yao Chunying Kang Key Laboratory of Horticultural Plant Biology(Ministry of Education) Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhanChina Department of Cell Biology and Molecular Genetics University of MarylandCollege ParkMDUSA The New Zealand Institute for Plant and Food Research Ltd AucklandNew Zealand

Flower and fruit development are two key steps for plant *** ABCE model for flower development has been well established in model plant species;however,the functions of ABCE genes in fruit crops are less *** this work,we identi fied an EMS mutant named R27 in woodland strawberry(Fragaria vesca),showing the conversion of petals,stamens,and carpels to sepaloid organs in a semidominant inheritance *** by sequencing revealed that the class E gene homolog FveSEP3(FvH4_4g23530)possessed the causative mutation in R27 due to a G to E amino acid change in the conserved MADS *** fvesep3^(CR) mutants generated by CRISPR/Cas9 displayed similar phenotypes to *** wild-type or mutated FveSEP3 in Arabidopsis suggested that the mutation in R27 might cause a dominant-negative *** analyses indicated that FveSEP3 physically interacted with each of the ABCE proteins in ***,both R27 and fvesep3^(CR) mutants exhibited parthenocarpic fruit growth and delayed fruit *** analysis revealed that both common and specific differentially expressed genes were identi fied in young fruit at 6-7 days post anthesis(DPA)of fvesep3 and pollinated wild type when compared to unpollinated wild type,especially those in the auxin pathway,a key hormone regulating fruit set in ***,we provided compelling evidence that FveSEP3 plays predominant E functions compared to other E gene homologs in flower development and that FveSEP3 represses fruit growth in the absence of pollination and promotes fruit ripening in strawberry.

关键词： straw CRISPR/Cas9 crops

血管化平行折叠腓骨瓣功能性重建下颌骨肿瘤术后骨缺损

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实用肿瘤杂志 2018年第1期33卷 76-80页

作者：蔡卓莺严奉国陈关福浙江大学医学院附属第二医院口腔颌面外科浙江杭州310009

目的探讨采用血管化平行折叠腓骨瓣的方法功能性重建肿瘤术后下颌骨大面积缺损,恢复外观及咀嚼功能的应用。方法收集下颌骨肿瘤扩大切除后需下颌骨区段性切除的患者13例,采用血管化平行折叠腓骨瓣重建下颌骨区段性缺损,恢复下颌骨外形... 详细信息

目的探讨采用血管化平行折叠腓骨瓣的方法功能性重建肿瘤术后下颌骨大面积缺损,恢复外观及咀嚼功能的应用。方法收集下颌骨肿瘤扩大切除后需下颌骨区段性切除的患者13例,采用血管化平行折叠腓骨瓣重建下颌骨区段性缺损,恢复下颌骨外形及咀嚼功能。结果 13例患者均顺利完成手术,术后移植腓骨瓣成活,患者对面部外观满意,发音正常,咀嚼和咬合关系基本正常,供区无并发症。术后6个月腓骨断端间愈合良好,种植术后咬合关系稳定。肿瘤无复发。结论血管化平行折叠腓骨瓣术式改善单段腓骨重建下颌骨所致的高度不足的缺陷,减轻二次手术创伤,减少游离植骨失败率,增加大面积骨缺损自体骨移植的适应证,为后期种植奠定基础,可有效改善患者术后外观及咀嚼功能。

关键词：下颌肿瘤/外科学下颌损伤/外科学外科皮瓣/血液供给腓骨/外科学腓骨/移植腓骨/血液供给

Statically accelerated experimental simulation on the deterioration of dynamic strength of rock

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Journal of University of Science and Technology Beijing 2005年第4期12卷 298-302页

作者： zhuoying Tan Meifeng cai Xingguang Zhao Civil and Environmental Engineering School University of Science and Technology Beijing Beijing 100083 China State Key Laboratory for High-Efficient Mining and Safety of Metal Mines （University of Science and Technology Beijing） Ministry of Education Beijing 100083 China School of Resource and Environment Guangxi University Nanning 530004 China

In order to reveal the strength deterioration law of rock mass under an acidified environment, a series of experiments on strength deterioration and the environmental effect on marble and diabase under the conditions of three kinds of acids （H2SO4, HNO3 and HC1） with three contents （1.0%, 5.0% and 10.0%） in a static fluid surrounding were carried out by single factor simulation. After the stones had been saturated in the above solutions for 90 days, an indoor weathering simulation had been observed for 300 days and its strength characteristics were measured under static/dynamic load and that results were compared with those under the natural condition. It is evident that the strength is emaciated greatly after the stones eroded by the liquids, whether under the static or dynamic load.

关键词： environmental acidification rock strength marble diabase

以胜任力为导向的案例式教学在口腔科学课程中的应用研究

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中国高等医学教育 2022年第11期 129-130页

作者：褚涵文许静蔡卓莺张琳范让刘雁鸣浙江大学医学院附属第二医院浙江杭州310009

目的:探讨基于胜任力为导向的案例式教学在口腔科学教学中的应用效果。方法:选取本校临床医学本科生按照班级随机分组,对照组采用传统教学法,实验组采用以胜任力为导向的案例式教学方式,比较两组的授课效果并进行评价。结果:实验组的测... 详细信息

目的:探讨基于胜任力为导向的案例式教学在口腔科学教学中的应用效果。方法:选取本校临床医学本科生按照班级随机分组,对照组采用传统教学法,实验组采用以胜任力为导向的案例式教学方式,比较两组的授课效果并进行评价。结果:实验组的测试成绩显著高于对照组,授课方式也更受学生青睐,差异具有统计学意义(P<0.05)。结论:以胜任力为导向的案例式教学应用于临床医学本科生的口腔科学课程有助于提升授课效果和教学质量。

关键词：胜任力导向案例式教学口腔科学

Application and reliability analysis of DPM system in site investigation of HK weathered granite

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Journal of University of Science and Technology Beijing 2005年第6期12卷 481-488页

作者： zhuoying Tan Meifeng cai Zhongqi Yue L.G. Tham C.F. Lee State Key Laboratory of High-Efficient Mining and Safety of Metal Mines （University of Science and Technology Beijing） Ministry of Education Beijing 100083 China Department of Civil Engineering University of Hong Kong Pokfulam Road Hong Kong China

A drilling process monitor （DPM） for ground characterization of weathered granite is presented. The monitor is portable and can be mounted on a hydraulic rotary drilling rig to record various drilling parameters in real time during normal subsurface investigation. The identification method for dominative and subsidiary interfaces has been established. The study reveals that the monitored drilling parameters are dependent on geotechnical materials and can be further applied to characterize ground interfaces. The t-test between manual logging and DPM logging has been carried out. The results show that the DPM has high accuracy in interfaces detection and well agreement with the manual logging. The findings show that the device and data analysis method are of potential application in subsurface drilling exploration in weathered granites. It also seems to have prospective uses in the determination of orebody boundary as well as in the detection of geohazards.

关键词： drilling process monitoring hydraulic rotary drill instrumented borehole drilling geotechnical engineering weathered granite

Instrumented borehole drilling for interface identification in intricate weathered granite ground engineering

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Journal of University of Science and Technology Beijing 2007年第3期14卷 195-199页

作者： zhuoying Tan Meifeng cai Z.Q. Yue L.G Tham C.F. Lee State Key Laboratory for High-Efficient Mining and Safety of Metal Mines （University of Science and Technology Beijing） Ministry of Education Beijing 100083. China Civil and Environmental Engineering School University of Science and Technology Beijing Beijing 100083 China Department of Civil Engineering The University of Hong Kong Hong Kong China

The successful application in drilling for HK simple weathered granite foundation has revealed its further use in instru- mented drilling system as a ground investigation tool in the detection of other lithology formations, geohazards, underground water, and boundary of orebody. To expand the further use and test the accuracy in identification of formation, an R-20 rotary-hydraulic drill rig was instrumented with a digital drilling process monitoring system （DPM） for drilling in an intricate decomposed granite site. In this test ground, the boreholes revealed that the weathered granite alternately changes between moderate and strong. The qualitative and quantitative analysis of the penetrating parameters, indicates the effective thrust force, rotary speed, flushing pressure, penetrating rate, and displacement of the bit fluctuate at ground interfaces. It shows that the parameters get a good response with the change of rock strength at the interfaces, which can reveal the change of the intricate granite formation. Besides, a variable-slope method has been established, for identification of dominative and subsidiary interfaces in the granite site. The result from a t-test shows that the confi- dence of the instrumented drilling system in identification of the geotechnical interfaces is up to 99%.

关键词： drilling process monitoring rotary-hydraulic drilling intricate weathered granite foundation

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风化花岗岩地层旋转钻进中的能量分析

岩石力学与工程学报 2007年第3期26卷 478-483页

作者：谭卓英岳中琦蔡美峰金属矿山高效开采与安全教育部重点实验室香港大学土木工程系

旋转钻进是岩土工程钻探的主要钻进方式,从能量守恒原理出发,对旋转钻进的能量进行分析。同时,在R-20液压式回转钻机上安装数字式钻孔过程监测(DPM)系统,在风化花岗岩地基工程中进行试验,并在监测数据的基础上对钻进能量进行分析计算。... 详细信息

旋转钻进是岩土工程钻探的主要钻进方式,从能量守恒原理出发,对旋转钻进的能量进行分析。同时,在R-20液压式回转钻机上安装数字式钻孔过程监测(DPM)系统,在风化花岗岩地基工程中进行试验,并在监测数据的基础上对钻进能量进行分析计算。研究结果表明,钻进过程中用于破碎岩石的能量主要来自钻进系统的动能,钻进系统用于破碎岩石的能量分配与地层强度特性有关。在风化程度较低或新鲜岩层中钻进时,破碎岩石98%以上的能量来自系统的动能,而轴压力推动钻头位移所给出的能量不到2%;在土层或全风化岩层中,轴压力所做的功达到22%以上,且明显随风化程度的增高而增大,说明钻进系统动能与轴力功可用以表征地层的可钻性,这为实时钻进能量用于地层的识别提供理论依据。

关键词：岩石力学旋转钻进钻进能量风化花岗岩地层识别

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岩土工程界面识别中的地层判别分类方法研究

岩石力学与工程学报 2008年第2期27卷 316-322页

作者：谭卓英王思敬蔡美峰北京科技大学土木与环境工程学院中国科学院地质与地球物理研究所

如何根据钻进过程参数曲线对钻进地层进行可钻性实时分级是仪器钻进系统发展和应用的关键技术。据此,对统计模式识别进行分析,并针对钻进参数样本数据的特点,采用最短距离聚类准则,分别建立类及分类数已知与类及分类数未知条件下的地层... 详细信息

如何根据钻进过程参数曲线对钻进地层进行可钻性实时分级是仪器钻进系统发展和应用的关键技术。据此,对统计模式识别进行分析,并针对钻进参数样本数据的特点,采用最短距离聚类准则,分别建立类及分类数已知与类及分类数未知条件下的地层判别分类及有效性检验方法;同时,在风化花岗岩金刚石钻进中采用钻进比功曲线对地层进行了分级研究。研究结果表明,在普通风化花岗岩地层中,该聚类分析方法分类性能良好、精度高、误判率低,所获得的分类结果与岩芯地质录孔的分类结果相吻合。该方法为岩土工程地层的自动判别分级提供了定量判别方法。

关键词：岩石力学钻进参数界面识别聚类分析风化花岗岩岩石分级

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变坡角浅层粘土斜坡稳定敏感性分析

北京科技大学学报 2005年第3期27卷 272-277页

作者：谭卓英蔡美峰北京科技大学土木与环境工程学院北京100083

用地球物理方法确定了滑坡体的坡底滑移面,重建了斜坡体,并根据坡角的变化划分了块段,运用力的递推原理,计算了滑坡体块段的稳定性系数.对坡角、含水率、内摩擦角、内聚力、静水压力、地震力及列车荷载等因素进行了分析,讨论了敏感性分... 详细信息

用地球物理方法确定了滑坡体的坡底滑移面,重建了斜坡体,并根据坡角的变化划分了块段,运用力的递推原理,计算了滑坡体块段的稳定性系数.对坡角、含水率、内摩擦角、内聚力、静水压力、地震力及列车荷载等因素进行了分析,讨论了敏感性分析中因子变化步长、范围及因子的相关性,获得了粘性土层容重、内摩擦角、内聚力与含水率之间以及内摩擦角与内聚力之间的耦合关系方程.在因子独立及关联作用下,分别对斜坡稳定性进行了单因素分析并确定了敏感性因子.结果表明,内摩擦角、内聚力、坡角及含水率是影响斜坡稳定性的主要敏感性因素.将滑坡因子作为独立变量和非耦合处理时,将弱化含水率及内聚力在稳定性分析中的影响.

关键词：变坡角粘土斜坡稳定性相关性敏感性分析