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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第8期10卷 572-579页

作者： Yong-liang zhuang xue ZHAO Ba-fang LI College of Food Science and Engineering Ocean University of China Qingdao 266003 China

To optimize the hydrolysis conditions to prepare hydrolysates of jellyfish umbrella collagen with the highest hydroxyl radical scavenging activity, collagen extracted from jellyfish umbrella was hydrolyzed with trypsin, and response surface methodology （RSM） was applied. The optimum conditions obtained from experiments were pH 7.75, temperature （T） 48.77 ℃, and enzyme-to-substrate ratio （[E]/[S]） 3.50%. The analysis of variance in RSM showed that pH and [E]/[S] were important factors that significantly affected the process （P〈0.05 and P〈0.01, respectively）. The hydrolysates of jellyfish umbrella collagen were fractionated by high performance liquid chromatography （HPLC）, and three fractions （HF-1〉3000 Da, 1000 Da〈HF-2〈3000 Da, and HF-3〈1000 Da） were collected. The HF-2 fraction had the highest hydroxyl radical scavenging activity with the highest yield compared with the other two fractions. Furthermore, HF-2 also showed the strongest Cu^2＋-chelating ability and the best tyrosinase-inhibitory activity.

关键词： Jellyfish umbrella collagen Hydrolysis Antioxidant activity Response surface methodology (RSM)

Effect of substrate temperature on microstructural and optical properties of ZnO films grown by pulsed laser deposition

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Rare Metals 2006年第2期25卷 161-165页

作者： HE Jianting zhuang Huizhao xue Chengshan WANG Shuyun HU Lijun xue Shoubin Institute of Semiconductor Shandong Normal University Jinan 250014 China

ZnO thin films were deposited on n-Si （111） at various substrate temperatures by pulsed laser deposition （PLD）. X-ray diffraction （XRD）, photoluminescence （PL）, Fourier transform infrared spectrophotometer （FTIR）, and scanning electron microscopy （SEM） were used to analyze the structure, morphology, and optical property of the ZnO thin films. An optimal crystallized ZnO thin film was obtained at the substrate temperature of 600℃. A blue shift was found in PL spectra due to size confinement effect as the grain sizes decreased. The surfaces of the ZnO thin films were more planar and compact as the substrate temperature increased.

关键词： semiconductor materials ZnO thin film PLD hexagonal wurtzite structure

Optical and Micro-Structural Properties of ZnO Thin Films Grown on Silicon Substrate by Pulsed Laser Deposition

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Journal of Rare Earths 2006年第z1期24卷 26-28页

作者： He Jianting zhuang Huizhao xue Chengshan Tian Deheng Wu Yuxin xue Shoubin Hu Lijun Institute of Semiconductor College of Physics and Electronics Shandong Normal University Jinan 250014 China Institute of Semiconductor College of Physics and Electronics Shandong Normal University Jinan 250014 China Institute of Semiconductor College of Physics and Electronics Shandong Normal University Jinan 250014 China Institute of Semiconductor College of Physics and Electronics Shandong Normal University Jinan 250014 China Institute of Semiconductor College of Physics and Electronics Shandong Normal University Jinan 250014 China Institute of Semiconductor College of Physics and Electronics Shandong Normal University Jinan 250014 China Institute of Semiconductor College of Physics and Electronics Shandong Normal University Jinan 250014 China

ZnO thin films were deposited on n-Si (111) at various substrate temperatures and oxygen pressures by pulsed laser deposition (PLD) using a Nd∶YAG laser with the wavelength of 1064 nm. X-ray diffraction (XRD), photoluminescence (PL), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to analyze the microstructure, optical property and morphology of the ZnO thin films. A comparatively optimal crystallized ZnO thin film was obtained at the substrate temperature of 600 ℃ in oxygen pressure of 50 mTorr. The intensity of the luminescence strongly depends on the stoichiometry of the film as well as the crystalline quality.

关键词： PLD ZnO substrate temperature oxygen pressure

Advances on Na-K liquid alloy-based batteries

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Journal of Energy Chemistry 2022年第8期31卷 313-323,I0009页

作者： Junwei Wu zhuang xue Lixuan Yuan Jilei Ye Qinghong Huang Lijun Fu Yuping Wu State Key Laboratory of Materials-Oriented Chemical Engineering College of Energy Science and EngineeringNanjing Tech UniversityNanjing 211816JiangsuChina

Sodium-potassium(Na^(-)K)liquid alloys attract increasing research attention,as an ideal alternative of Li metal for metal-based batteries,attributing to their high abundance,low redox potential,high capacity,and dendrite-free *** addition,the liquid and self-healing features of Na^(-)K alloys endow good electrode/electrolyte interfacial *** recent advances on the Na^(-)K liquid alloy-based batteries(NKBs)are reviewed *** anode designs for immobilization of the liquid alloy are *** influences of the electrolyte and cathode materials on the battery performances are *** addition,considering the co-existence of both K^(+)and Na^(+)in the electrolyte,the working mechanisms of the NKBs are *** also show that despite the improvement,challenges of the NKBs *** compatibility between Na^(-)K liquid alloy and electrolyte,as well as disputed working mechanisms,request detailed surface analyses of the liquid alloy and local element distribution evolution in the *** review would shed light on the fundamental understanding of Na^(-)K alloy electrochemistry and the development of dendrite-free metal-based energy storage systems with high energy density.

关键词： Sodium-potassium alloy Liquid anode Metal battery Dendrite-free Mechanism

Characterization and Phylogenetic Analysis of Lectin Gene cDNA Isolated from Sea Cucumber (Apostichopus japonicus) Body Wall

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Journal of Ocean University of China 2017年第6期16卷 1148-1156页

作者： xue zhuang LI Hui LIU Yang ZHOU Wei SUN Jing WANG Xiuli College of Fisheries and Life Science Dalian Ocean University Dalian 116023 P. R. China Liaoning Province Academy of Analytic Science Shenyang 110015 P. R. China

As a ‘living fossil' of species origin and ‘rich treasure' of food and nutrition development, sea cucumber has received a lot of attentions from researchers. The cDNA library construction and EST sequencing of blood had been conducted previously in our lab. The bioinformatic analysis provided a gene fragment which is highly homologous with the genes of lectin family, named AjL(Apostichopus japonicus lectin). To characterize and determine the phylogeny of AjL genes in early evolution, we isolated a fulllength cDNA of lectin gene from the body wall of A. japonicus. The open reading frame of this gene contained 489 bp and encoded a 163 amino acids secretory protein being homologous to lectins of mammals and aquatic organisms. The deduced protein included a lectin-like domain. SDS-PAGE analysis showed that AjL migrated as a specific band(about 36.09 kDa under reducing), and agglutinated against rabbit red blood cells. AjL was similar to chain A of CEL-IV in space structure. We predicted that AjL may play the same role of CEL-IV. Our results suggested that more than one lectin gene functioned in sea cucumber and most of other species, which was fused by uncertain sequences during the evolution and encoded different proteins with diverse functions. Our findings provided the insights into the function and characteristics of lectin genes invertebrates. The results will also be helpful for the identification and structural, functional, and evolutionary analyses of lectin genes.

关键词： sea cucumber agglutination activity lectin space structure

Fabrication of ZnO films by radio frequency magnetron sputtering and annealing

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Rare Metals 2005年第3期24卷 267-271页

作者： GAO Haiyong zhuang Huizhao xue Chengshan WANG Shuyun DONG Zhihua HE Jianting Institute of Semiconductor Shandong Normal University Jinan 250014 China

ZnO thin films were deposited on Si（111） substrates through a radio frequency （rf） magnetron sputtering system. Then the samples were annealed at different temperatures in air ambience and ammonia ambience respectively. The structure and composition of the ZnO films were studied by X-ray diffraction （XRD） and X-ray photoelectron spectroscopy （XPS）. The morphology of the samples was studied by scanning electron microscopy （SEM）. Measured results show that ZnO films with hexagonal wurtzite structure were grown on Si（111） substrates when annealed in the two ambiences. The volatilization process of ZnO in the ammonia ambience at high temperature was discussed and the mechanism of the reaction was analyzed.

关键词： ZnO films radio frequency magnetron sputtering annealing ammonia ambience buffer layers

Guazia, the earliest ovule without cupule but with unique integumentary lobes

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National Science Review 2022年第4期9卷 13-20页

作者： De-Ming Wang Le Liu Yi Zhou Min Qin Mei-Cen Meng Yun Guo Jin-zhuang xue Key Laboratory of Orogenic Belts and Crustal Evolution Department of GeologyPeking University School of Geoscience and Surveying Engineering China University of Mining and Technology (Beijing) Institute of Geology and Paleontology Linyi University Institute of Deep Time Terrestrial Ecology Institute of PalaeontologyYunnan Key Laboratory of Earth System ScienceYunnan University

The earliest ovules in the Late Devonian(Famennian) are surrounded by a cupule that is involved in both protection and pollination, and generally have free integumentary lobes. Here we report a new taxon from the Famennian of China, Guazia dongzhiensis gen. et sp. nov. The terminally borne ovule is apparently acupulate(without cupule) and has four radially arranged wing-like integumentary lobes that are extensively fused, and folded lengthwise and inwards. Guazia provides evidence that not all Devonian seeds possess a cupule and it increases their diversity in integumentary lobes. This genus also suggests that the integuments develop new functions, probably including wind dispersal at the expense of the cupules.

关键词： ovule cupule integument Devonian seed plants

First Human Infection with Avian Influenza H9N2—Guangdong Province,China,2020

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China CDC weekly 2020年第29期2卷 545-548页

作者： Qianfang Guo Lirong Zou Jianxiang Yu Yingchao Song Lijun Liang xue zhuang Tie Song Jie Wu Guangdong Provincial Center of Disease Control and Prevention GuangzhouGuangdongChina Southern Medical University School of Public HealthGuangzhouGuangdongChina

A case of avian influenza H9N2 was detected in a human in Zhuhai City,Guangdong Province through routine influenza surveillance on March 30,*** patient was a 3-year-old child who developed fever and cough on March 22.A pharyngeal swab was collected on March 23,and the novel coronavirus nucleic acid test was *** March 26,the pharyngeal swab was sent to Zhuhai CDC for influenza virus tests and was found to be H9 influenza A positive,and on March 30,Guangdong Provincial CDC reexamined the pharyngeal swabs as positive for influenza A(H9N2).

关键词： Guangdong routine Province,China

MAIN FUNCTIONS AND EVALUATION OF CUTI'S COMPLEX——Simulated Saturation Diving Chamber

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China Ocean Engineering 1990年第4期5卷 459-468页

作者： Zhang Longbao , zhuang Qinhong xue Liqun Associate Professor, Chinese Underwater Technology Institute (CUTI), Shanghai Engineer, Chinese Underwater Technology Institute (CUTI), Shanghai Chinese Underwater Technology Inst Shanghai China

The 300 msw Simulated Saturation Diving Chamber Complex was designed and built by ourselves. It was completed at the end of 1985. A 300 msw trimixed saturation diving experiment was successfully conducted in the complex by Chinese Underwater Technology Institute(CUTI) from May 22 to June 12 of 1987. During the experiment, 4 divers habitated in the complex for 20 days, and they performed 16 person-time excursion dives and 8 other tests. The result of the experiment indicates that the complex is well designed, suitably configurated, wholly integrated and steadily run, as well as of low leakage. The main functions of the complex have approached to those of the same kind in the world. The complex can be used as a basic facility for serving the nation's saturation diving technology, underwater operation, personnel training, etc.

关键词： ATA Simulated Saturation Diving Chamber MAIN FUNCTIONS AND EVALUATION OF CUTI’S COMPLEX

Lamprey buccal gland secretory protein-2 (BGSP-2) inhibits human T lymphocyte proliferation

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Current Zoology 2010年第2期56卷 252-258页

作者： Jing SUN Shuiyan YU zhuang xue Cenjie LIU Yu WU Xin LIU Qingwei LI Institute of Marine Genomics ＆ Proteomics Liaoning Norrnal University Dalian 116029 China

Lamprey is a representative of the agnathans, the most ancient class of vertebrates. Parasitic lampreys secrete anticoagulant from their buccal glands and prevent blood coagulation of host fishes. We identified a buccal gland secretory protein-2 （BGSP-2） from a buccal gland cDNA library of Larnpetrajaponica. The full-length BGSP-2 gene was cloned and the recombinant BGSP-2 protein was generated. The role of BGSP-2 on lymphocyte proliferation was studied by examining its effects on human T lymphocytes. We found that lamprey BGSP-2 was able to effectively block the proliferation of T cells in vitro by inducing G1/S cell cycle arrest. Furthermore, it inhibited the proliferation of hmnan T lymphocytes stimulated by phytohemagglutinin （PHA） at a minimum concentration of 0.1μg/ml. Our data suggest that lamprey BGSP-2 is able to block the mitosis of human T lymphocytes at the G1/S point, and has the potential of anti-proliferative effect on PHA-activated T lymphocytes.

关键词： Lampetra japonica BGSP-2 Cell cycle T lymphocyte Proliferation