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Occlusion of the middle cerebral artery Guidance by screen imaging using an EDA-H portable medium-soft electronic endoscope

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Neural Regeneration Research 2011年第23期6卷 1806-1809页

作者： xingbao zhu Junli Luo Song Liu Dongping Li Min Li Quanshui Fan Department of Neurosurgery Kunming General Hospital Chengdu Military Command of Chinese PLA Kunming 650032 Yunnan Province China Disease Prevention ＆ Control Centre Chengdu Military Command of Chinese PLA Kunming 650032 Yunnan Province China

The present study aimed to verify the practicability of performing screen-based surgical operations under the guidance of a real-time viewing and recording system （RTVRS） using a portable medium-soft electronic endoscope （pmsEE）. The middle cerebral artery in rats was occluded under screen-imaging guidance using a pmsEE RTVRS to reproduce an animal model of human cerebral infarction. The screen imaging of the pmsEE RTVRS was clear, life-like, stereoscopic and synchronous with the actual operation. Screen-imaging guidance led to an accurate, smooth, minimally invasive and comparatively easy surgical procedure. The surgical success rate, time of model establishment, neurological function scores, and infarct volume were similar to those using an operating microscope. These results indicate that the self-designed pmsEE RTVRS could be utilized for portable endoscopic screen-based surgical operations.

关键词： portable medium-soft endoscope screen-based surgical operation occlusion of middle cerebral artery cerebral infarction

In vitro construction of a recombinant human embryonic brain-derived neurotrophin-4 gene and pEGFP-N1 vector

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Neural Regeneration Research 2009年第4期4卷 312-315页

作者： Jintao Li Qi Yan xingbao zhu Dan Xu Tinghua Wang Huatang Zhang Jia Liu Institute of Neuroscience Kunming Medical College Kunming 650031 Yunnan Province China Department of Minimally Invasive Neurosurgery First Hospital of Kunming Medical College Kunming 650032 Yunnan Province China Department of Neurosurgery Kunming General Hospital of Chengdu Military Area Command of Chinese PLA Kunming 650032 Yunnan Province China Kunming Institute of Zoology Chinese Academy of Sciences Kunming 650223 Yunnan Province China

BACKGROUND： Neurotrophin-4 （NT-4） can promote neuronal growth, development, differentiation, maturation, and survival. NT-4 can also improve recovery and regeneration of injured neurons, but cannot pass through the blood-brain barrier, which limits its activity in the central nervous system. Delivering NT-4 into the central nervous system v/a cells or vectors may have therapeutic benefit. OBJECTIVE： To construct a recombinant vector with a human embryonic brain-derived NT-4 gene and pEGFP-NI. DESIGN, TIME AND SETTING： Neural genetic engineering experiment. The study was performed at the Neuroscience Institute of Kunming Medical College between October 2007 and March 2008. MATERIALS： The pEGFP-N1 plasmid vector was provided by Kunming Institute of Zoology, Chinese Academy of Sciences; embryonic brain tissues were provided by the First Affiliated Hospital of Kunming Medical College. TRIzol RNA extraction Kit was purchased from Sigma （USA）, One Step RNA PCR Kit （AMV） etc. were from Takara （Dalian, China）. METHODS： Total RNA was extracted from human embryonic brain tissues using Trizol. The agarose gel electrophoresis showed two bands： 18 S and 28 S, which were essential subunits of total RNA. The human NT-4 DNA was obtained via RT-PCR and inserted into the pEGFP-N1 vector using ligation and transformation reaction. MAIN OUTCOME MEASURES： The sequencing results of the DNA in the recombinant of NT-4- pEGFP-NI. RESULTS： The NT-4-pEGFP-N1 vector was sequence-verified and showed the expected molecular weight. CONCLUSION： The recombinant of NT-4-pEGFP-N1 was constructed successfully in vitro.

关键词： human embryonic brain neurotrophin-4 gene construction in vitro

Screen-imaging guidance using a modified portable video macroscope for middle cerebral artery occlusion

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Neural Regeneration Research 2012年第12期7卷 912-916页

作者： xingbao zhu Junli Luo Yun Liu Guolong Chen Song Liu Qiangjin Ruan Xunding Deng Dianchun Wang Quanshui Fan Xinghua Pan Department of Neurosurgery Kunming General Hospital Chengdu Military Area Command of Chinese PLA Kunming 650032 Yunnan Province China Disease Prevention ＆ Control Centre Chengdu Military Area Command of Chinese PLA Kunming 650032 Yunnan Province China Animal Experimental Centre Department of Clinical Research Kunming General Hospital Chengdu Military Area Command of Chinese PLA Kunming 650032 Yunnan Province China

The use of operating microscopes is limited by the focal *** using these instruments cannot simultaneously view and access the surgical field and must choose one or the *** longer focal length （more than 1 000 mm） of an operating telescope permits a position away from the operating field,above the surgeon and out of the field of *** gives the telescope an advantage over an operating *** developed a telescopic system using screen-imaging guidance and a modified portable video macroscope constructed from a Computar MLH-10 × macro lens,a DFK-21AU04 USB CCD Camera and a Dell laptop computer as monitor *** system was used to establish a middle cerebral artery occlusion model in *** showed that magnification of the modified portable video macroscope was appropriate （5-20 ×） even though the Computar MLH-10 × macro lens was placed 800 mm away from the operating field rather than at the specified working distance of 152.4 mm with a zoom of 1-40 ×.The screen-imaging telescopic technique was clear,life-like,stereoscopic and matched the actual ***-imaging guidance led to an accurate,smooth,minimally invasive and comparatively easy surgical *** rate of the model establishment evaluated by neurological function using the modified neurological score system was 74.07%.There was no significant difference in model establishment time,sensorimotor deficit and infarct volume *** findings indicate that the telescopic lens is effective in the screen surgical operation mode referred to as ＂long distance observation and short distance operation＂ and that screen-imaging guidance using an modified portable video macroscope can be utilized for the establishment of a middle cerebral artery occlusion model and micro-neurosurgery.

关键词： portabie video macroscope screen-imaging guidance telescopic surgery middle cerebral artery occlusion cerebral infarction

Pre-stroke DNA immunization against neurite growth inhibitors is beneficial to the recovery from focal cerebral ischemia in rats

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Neural Regeneration Research 2007年第9期2卷 513-518页

作者： xingbao zhu Jasmine Lee Jill Wong Wan Loo Tan Zhongtang Feng Tinghua Wang Zhicheng Xiao Ivan Ng Department of Neurosurgery Kunming General Hospital of Chinese PLA Kunming 650032 Yunnan Province China Brain Injury Research Laboratory Department of Neurosurgery National Neuroscience Institute Singapore 408433 Institute of Neuroscience Kunming Medical College Kunming 650031 Yunnan Province China Institute of Molecular and Cell Biology Singapore 117608 Department of Anatomy Faculty of Medicine National University of Singapore Singapore 119074 Department of Clinical Research Singapore General Hospital Singapore 169608

BACKGROUND： Inhibitory signals, i.e. neurite growth inhibitors （NGIs）, presenting on central nervous system （CNS） myelin have been shown to play a crucial role in inhibiting lesioned axonal sprouting and leading to less functional recovery. Vaccines targeting NGIs may provide multifactorial protection against brain insults by overcoming the inhibitory effects of these NGIs and boosting the immune repair mechanisms of body. OBJECTIVE： To evaluate the effect of pre-stroke DNA immunization against NGIs on the rehabilitation for sensorimotor function of rat models of focal cerebral ischemia. DESIGN： A completely randomized design, and controlled experiment. SETTING： Brain Injury Research Laboratory, Department of Neurosurgery, National Neuroscience Institute, Singapore. MATERIALS： Sixty adult male Sprague-Dawley rats ranging in age from 45 to 120 days and in body mass from 180 to 250 g were provided by the Animal Center of Department of Anatomy, Faculty of Medicine, National University of Singapore. pcDNA3.1（＋）-neurite growth inhibitors （pcDNA-NGIs） a gift was provided by Dr. Xiao from the Department of Clinical Research, Singapore General Hospital, Singapore. METHODS： The experiment was carried out at Brain Injury Research Laboratory, Department of Neurosurgery, National Neuroscience Institute, Singapore from August 2003 to April 2005. ① The involved rats were randomized into 3 groups： model group （group A）, pcDNA3.1（＋） group （group B） and pcDNA-NGIs group （group C）, with 20 rats in each group. Left focal cerebral ischemia was permanently induced through middle cerebral artery occlusion with the assistance of an operating microscope. Successful middle cerebral artery occlusion was determined by a 20% decrease to baseline in the ipsilateral cerebral blood flow. 100 μg of pcDNA-NGIs eluted in phosphate-buffered saline （PBS） was intramuscularly injected into the tibial muscle once a week before middle cerebral artery occlusion for 6 weeks in group C. As control, pcD

关键词： stroke growth inhibitors cerebral ischemia

Poststroke DNA immunization against neurite growth inhibitors is beneficial to the recovery from local cerebral ischemia in rats

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Neural Regeneration Research 2007年第2期2卷 65-69页

BACKGROUND： Inhibitory signals, i.e. neurite growth inhibitors （NGIs）, presenting on central nervous system （CNS） myelin have been shown to play a crucial role in inhibiting lesioned axonal sprouting and leading to less functional recovery. Vaccines targeting NGIs may provide multifactorial protection against brain insults by overcoming the inhibitory effects of these NGIs and boosting the body＇s immune repair mechanisms. OBJECTIVE： To evaluate the effect of poststroke DNA immunization against NGIs on the rehabilitation for sensorimotor function of rat models of local cerebral ischemia. DESIGN： Completely randomized grouping design, and controlled experiment. SETTING： Brain Injury Research Laboratory, Department of Neurosurgery, National Neuroscience Institute, Singapore. MATERIALS： Sixty adult male Sprague-Dawley rats ranging in age from 45 to 120 days and in weight from 180 to 250 grams were provided by Animal Center of Department of Anatomy, Faculty of Medicine, National University of Singapore. pcDNA3.1（＋）-neurite growth inhibitors （pcDNA-NGIs） a gift was provided by Dr. Xiao from Department of Clinical Research, Singapore General Hospital, Singapore. METHODS： The experiment was carried out at Brain Injury Research Laboratory, Department of Neurosurgery, National Neuroscience Institute, Singapore from August 2003 to April 2005. （1）The involved rats were randomized into 3 groups： pcDNA-NGIs group （group A）, pcDNA3.1 （＋） group （group B） and model group （group C）, with 20 rats in each group. Left focal cerebral ischemia （FCI） was permanently induced through middle cerebral artery occlusion （MCAO） with the assistance of an operating microscope. Successful MCAO was determined by a 20% decrease to baseline in the ipsilateral cerebral blood flow. 100 μg of pcDNA-NGIs eluted in phosphate-buffered saline （PBS） was intramuscularly injected into the tibial muscle once a week after MCAO for 6 weeks in group A. As control, pcDNA3.1 （＋） was also administrated in the sam

关键词： stroke(TCM) growth inhibitors, cerebral ischemia

Improving discharge voltage and ion storage dynamic in polyaniline via modulation of carrier charge density for magnesiummetal batteries

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Nano Research 2024年第7期17卷 6168-6175页

作者： Ping Luo Feiyang Chao Chunli Zuo Wenwei Zhang Fangyu Xiong Zhen Huang Dongyao zhu Gongtao Yu Wenhui Zhong xingbao Chen Han Tang Xiujuan Wei Qinyou An Hubei Provincial Key Laboratory of Green Materials for Light Industry School of Materials and Chemical EngineeringHubei University of TechnologyWuhan 430068China State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan 430070China College of Materials Science and Engineering Chongqing UniversityChongqing 400044China Institute for Sustainable Transformation School of Chemical Engineering and Light IndustryGuangdong University of TechnologyGuangzhou 510006China

Rechargeable magnesium-metal batteries(RMBs)have gained much attention due to their abundant resources as well as high ***,the high charge density of Mg^(2+)is one of the main reasons for the slow kinetics performance of RMBs,and modulation of the charge density is an important strategy to improve the kinetics and electrochemical performance of ***,we report on the conductive polymer polyaniline(PANI)for RMBs,which is found to have excellent kinetics and high discharge voltage when storing MgCl^(+).In the storage of MgCl^(+),PANI exhibits a high average discharge voltage platform is 2.3 V ***^(2+)/Mg,which is higher than that in storage of Mg^(2+).We demonstrated the reversible intercalation/de-intercalation of MgCl^(+)in PANI accompanying with the reversible transformation between the quinone ring(C–C,–N=)and the benzene ring(C=C,–NH–)during charging and *** functional theory calculation reveals that PANI exhibit higher voltages(2.25 V vs.1.82 V)along with lower diffusion energy barriers(1.27 eV vs.1.55 eV)for MgCl^(+)storage compared to Mg^(2+)*** work refines the storage mechanism of PANI in RMBs and provides new guidelines for the application of PANI in RMBs.

关键词： rechargeable magnesium-metal batteries cathode materials polyaniline MgCl+storage