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Comparison of the Pathogenicity of Neisseria meningitidis Isolates of Hyperinvasive Sequence Type 7 Belonging to Serogroups A,B,C,and X

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Biomedical and Environmental Sciences 2020年第2期33卷 114-122页

作者： GUO Peng Bo zhu bing qing XU Li GAO Wan Ying GAO Yuan LIANG Hao ZHANG Mao Jun SHAO zhu Jun State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Hangzhou 310003ZhejiangChina

Objective To compare the pathogenicity of isolates of sequence type 7(ST-7)Neisseria meningitidis(***)belonging to four different serogroups(A,B,C,and X).Methods Four ST-7 *** isolates serogrouped as A,B,C,and X and characterized by different capsule structures,were examined for their adhesion and invasion properties,and their ability to induce cytokine release and apoptosis in the host cell(the A549 cell line).Results Among the four ST-7 *** isolates,the serogroup A isolate possessed the strongest adhesion and invasion *** isolate also induced the release of the highest levels of the proinflammatory mediators interleukin-6,interleukin-1β,and interferon,and the highest apoptosis rate in the host ***,there was no significant difference in interleukin-8 and tumor necrosis factor-αsecretion between the four *** on the findings,the serogroup X *** isolate had the weakest pathogenicity,whereas there was almost no difference in the pathogenicity of the isolates from serogroups B and *** The differences in the capsular structure of the four isolates of ST-7 *** affected their pathogenic *** findings also imply that the hyperinvasive ST-7 *** lineage may include hypoinvasive isolates.

关键词： ST-7 N.meningitidis Capsule Pathogenicity Adaptation

A Cross-sectional Survey Assessing Carriage of Streptococcus pneumoniae in a Healthy Population in Xinjiang Uygur Autonomous Region of China

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Biomedical and Environmental Sciences 2018年第3期31卷 233-237页

作者： XIE Na CHEN Zhao Yun CHEN Tao zhu bing qing XU Li GAO Yuan ZHANG Ai Yu ZHAO Pan LIU Ji Wen SHAO zhu Jun Department of Public Health Xinjiang Medical University Urumqi 830054 Xinjiang China National Institute for Communicable Disease Control and Prevention State Key Laboratory for Infectious Disease Prevention and Control Chinese Center for Disease Control and Prevention Beijing 102206 China Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region Urumqi 830011 Xinjiang China Medical Laboratory Center the First Affiliated Hospital of Xinjiang Medical University Urumqi 830054 Xinjiang China Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Hangzhou 310058 Zhejiang China

The carriage rate and serotype distribution of Streptococcus pneumoniae（***）in a healthy population in China remains *** this study,we collected the oropharyngeal swabs from513 individuals in Xinjiang,***-time PCR t... 详细信息

关键词： Cross-sectional survey Streptococcus pneumoniae carriage Real-time PCR Xinjiang China

Proteome analysis of round-headed and normal spermatozoa by 2-D fluorescence difference gel electrophoresis and mass spectrometry

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Asian Journal of Andrology 2009年第6期11卷 683-694页

作者： Ting-Ting Liao Zhen Xiang Wen-bing zhu Li-qing Fan Institute of Human Reproduction and Stem Cell Engineering Central South University Changsha 410078 China

Globozoospermia is a severe form of teratozoospermia characterized by round-headed spermatozoa with an absent acrosome, an aberrant nuclear membrane and midpiece defects. Globozoospermia is diagnosed by the presence of 100% round-headed spermatozoa on semen analysis, and patients with this condition are absolutely infertile. The objective of this study was to investigate the differences in protein expression between human round- headed and normal spermatozoa. Two-dimensional （2-D） fluorescence difference gel electrophoresis （DIGE） coupled with mass spectrometry （MS） was used in this study. Over 61 protein spots were analysed in each paired normal/round-headed comparison, using DIGE technology along with an internal standard. In total, 35 protein spots identified by tandem mass spectrometry （MS/MS） exhibited significant changes （paired t-test, P 〈 0.05） in the expression level between normal and round-headed spermatozoa. A total of nine proteins were found to be upregulated and 26 proteins were found to be downregulated in round-headed spermatozoa compared with normal spermatozoa. The differentially expressed proteins that we identified may have important roles in a variety of cellular processes and structures, including spermatogenesis, cell skeleton, metabolism and spermatozoa motility.

关键词： differential protein globozoospermia mass spectrometry (MS) two-dimensional difference gel electrophoresis (2-D DIGE)

Effect of pyrite content on chalcopyrite flotation under different regrinding conditions

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 49-57页

作者： Zejun Wang qing Shi Guofan Zhang Yuxuan zhu Binbin Li School of Minerals Processing and Bioengineering Central South UniversityChangsha 410083China Key Laboratory of Hunan Province for Clean and Efficient Utilization of Strategic Calcium-containing Mineral Resources Central South UniversityChangsha 410083China

This study aimed to investigate the effect of varying pyrite(Py)content on copper(Cu)in the presence of different regrinding conditions,which were altered using different types of grinding media:iron,ceramic balls,and their mixture,followed by flotation in the cleaner *** flotation performance of rough Cu concentrate can be improved by changing the regrinding conditions based on the Py *** electron microscopy,X-ray spectrometry,ethylenediaminetetraacetic acid disodium salt extraction,and X-ray photoelectron spectroscopy studies illustrated that when the Py content was high,the use of iron media in regrinding promoted the generation of hydrophilic Fe OOH on the surface of Py and improved the Cu *** ceramic medium with a low Py content prevented excessive Fe OOH from covering the surface of chalcopyrite(Cpy).Electrochemical studies further showed that the galvanic corrosion current of Cpy-Py increased with the addition of Py and became stronger with the participation of iron media.

关键词： chalcopyrite regrinding media pyrite galvanic corrosion flotation

Secure beamforming and deployment design for rate-splitting multiple access-based UAV communications

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Science China(Information Sciences) 2025年第1期68卷 342-356页

作者： bing WANG Xiaofeng TAO Shujun HAN Huici WU Kai YANG zhu HAN National Engineering Research Center of Mobile Network Technologies Beijing University of Posts and Telecommunications Peng Cheng Laboratory Department of Electrical and Computer Engineering University of Houston

Rate-splitting multiple access(RSMA) has recently gained attention as a potential robust multiple access(MA)scheme for upcoming wireless networks. Given its ability to efficiently utilize wireless resources and design interference management strategies, it can be applied to unmanned aerial vehicle(UAV) networks to provide convenient services for large-scale access ground users. However, due to the line-of-sight(LoS) broadcast nature of UAV transmission, information is susceptible to eavesdropping in RSMA-based UAV networks. Moreover, the superposition of signals at the receiver in such networks becomes complicated. To cope with the challenge, we propose a two-user multi-input single-output(MISO) RSMA-based UAV secure transmission framework in downlink communication networks. In a passive eavesdropping scenario, our goal is to maximize the sum secrecy rate by optimizing the transmit beamforming and deployment location of the UAV-base station(UAV-BS),while considering quality-of-service(QoS) constraints, maximum transmit power, and flight space limitations. To address the non-convexity of the proposed problem, the optimization problem is first decoupled into two subproblems. Then, the successive convex approximation(SCA) method is employed to solve each subproblem using different propositions. In addition, an alternating optimization(AO)-based location RSMA(L-RSMA) beamforming algorithm is developed to implement joint optimization to obtain the suboptimal solution. Numerical results demonstrate that(1) the proposed L-RSMA scheme yields a28.97% higher sum secrecy rate than the baseline L-space division multiple access(SDMA) scheme;(2) the proposed L-RSMA scheme improves the security performance by 42.61% compared to the L-non-orthogonal multiple access(NOMA) scheme.

关键词： rate-splitting multiple access UAV communication precoding optimization deployment location physical layer security

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Trinuclear gold-catalyzed site-selective alkylation of peptides

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Science China Chemistry 2025年第1期68卷 249-256页

作者： qing-Yun Fang Chengyihan Gu Yinghan Chen Wencheng Yan Yong Liang Weipeng Li Chengjian zhu Jie Han Jin Xie State Key Laboratory of Coordination Chemistry Jiangsu Key Laboratory of Advanced Organic Materials Chemistry and Biomedicine Innovation Center (Chem BIC) School of Chemistry and Chemical Engineering Nanjing University Green Catalysis Center College of Chemistry Zhengzhou University State Key Laboratory of Natural Medicines China Pharmaceutical University

Selective modification of peptides has always been a challenging issue. The selective alkylation of peptides can alter the biological activity and physical properties of molecules, and it has gained significant research interest. We herein disclose an intriguing gold-catalyzed alkylation of peptides using bench-stable unactivated alkyl bromides under photoredox catalysis. A wide range of structurally diverse primary, secondary and tertiary alkyl bromides serve as effective coupling partners to precisely connect with the α-C(sp3)–H in glycine residue of peptides. This protocol demonstrates an exceptionally broad scope for alkyl halides and excellent tolerance for a wide range of useful functional groups. A series of relay transformations to construct cyclic peptides, to proceed click reaction and to realize peptide fluorescence labeling further enhance its synthetic practicality. In addition, mechanistic studies and density functional theory(DFT) calculations reveal an inner-sphere and subsequent outersphere single electron transfer(SET) mechanism.

关键词： gold catalysis peptide alkylation C-C coupling radical

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血清杀菌试验和酶联免疫吸附试验测定C群脑膜炎奈瑟菌血清抗体滴度比较

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中华流行病学杂志 2012年第5期33卷 521-524页

作者：朱兵清徐丽周海健邵祝军中国疾病预防控制中心传染病预防控制所国家重点实验室北京102206

目的比较血清杀菌试验（SBA）和酶联免疫吸附试验（ELISA）N定两种C群脑膜炎奈瑟菌（Nm）疫苗免疫后血清抗体滴度的差异。方法采用SBA测定75名未免疫健康成年人（40-70岁）血清、143名3-8月龄婴儿及194名3-5岁儿童NmA和C群结合疫苗或A＋... 详细信息

目的比较血清杀菌试验（SBA）和酶联免疫吸附试验（ELISA）N定两种C群脑膜炎奈瑟菌（Nm）疫苗免疫后血清抗体滴度的差异。方法采用SBA测定75名未免疫健康成年人（40-70岁）血清、143名3-8月龄婴儿及194名3-5岁儿童NmA和C群结合疫苗或A＋C群多糖疫苗免疫前后血清中Nm杀菌抗体水平，然后用ELISA测定相应血清的Nm特异性IgG含量，并利用线性相关与回归分析两种测定结果的相关性。结果未免疫健康成年人血清中Nm杀菌抗体水平和特异性IgG含量之间的相关性较高（r=0．81433，P〈0．001）；3-8月龄婴儿和3-5岁儿童接种结合／多糖疫苗前，Nm杀菌抗体水平和特异性IgG含量之间相关性较差（3-8月龄：r=0．14064，P〉0．100／r=0．2899，P〈0．05；3-5岁：r=0．54040，P〈0．05／r=0．19436，P〈0．05），接种1剂结合疫苗后，杀菌抗体水平和特异性IgG含量之间的相关性较好（r=0．80938，P〈0．001和r=0．83723，P〈0．001）；接种多糖疫苗后，杀菌抗体水平和特异性IgG含量之间的相关性较差（r〈0．50000）。结论健康成年人血清和结合疫苗免疫后的婴幼儿血清中特异性C群NmIgG含量可以间接反映血清杀菌抗体水平，ELISA方法可以替代SBA。但ELISA检测方法不适用于3-8月龄婴儿多糖疫苗免疫后的效果评价。

关键词： C群脑膜炎奈瑟菌血清杀菌试验酶联免疫吸附试验

Characteristic changes in astrocyte properties during astrocyte-to-neuron conversion induced by NeuroD1/Ascl1/Dlx2

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Neural Regeneration Research 2025年第6期20卷 1801-1815页

作者： qing He Zhen Wang Yuchen Wang Mengjie zhu Zhile Liang Kanghong Zhang Yuge Xu Gong Chen GHM Institute of CNS Regeneration Jinan UniversityGuangzhouGuangdong ProvinceChina

Direct in vivo conversion of astrocytes into functional new neurons induced by neural transcription factors has been recognized as a potential new therapeutic intervention for neural injury and degenerative disorders. However, a few recent studies have claimed that neural transcription factors cannot convert astrocytes into neurons, attributing the converted neurons to pre-existing neurons mis-expressing transgenes. In this study, we overexpressed three distinct neural transcription factors––NeuroD1, Ascl1, and Dlx2––in reactive astrocytes in mouse cortices subjected to stab injury, resulting in a series of significant changes in astrocyte properties. Initially, the three neural transcription factors were exclusively expressed in the nuclei of astrocytes. Over time, however, these astrocytes gradually adopted neuronal morphology, and the neural transcription factors was gradually observed in the nuclei of neuron-like cells instead of astrocytes. Furthermore,we noted that transcription factor-infected astrocytes showed a progressive decrease in the expression of astrocytic markers AQP4(astrocyte endfeet signal), CX43(gap junction signal), and S100β. Importantly, none of these changes could be attributed to transgene leakage into preexisting neurons. Therefore, our findings suggest that neural transcription factors such as NeuroD1, Ascl1, and Dlx2 can effectively convert reactive astrocytes into neurons in the adult mammalian brain.

关键词： aquaporin-4 Ascl1 astrocyte cortex Dlx2 gap junction glia-to-neuron conversion neural regeneration NeuroD1 reprogramming

杜莫氏军团菌对上皮细胞A549粘附侵袭和胞内生长能力的研究(英文)

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中国人兽共患病学报 2012年第6期28卷 522-527页

作者：秦天任红宇朱兵清邵祝军中国疾病预防控制中心传染病预防控制所呼吸道传染病室传染病预防控制国家重点实验室北京102206

目的探明杜莫氏军团菌(Legionella dumoffii,***)对上皮细胞A549的粘附、侵袭和胞内生长能力。方法实验使用菌株为*** TEX-KL(ATCC 33343)、*** NY23(ATCC 33279)和嗜肺军团菌***-phila philadelphila-1(ATCC 33155)。配制1×108菌悬液,将其以100MOI(Multiplicity of Infection)的比例与肺泡上皮细胞A549相互作用。通过吉曼尼兹染色和菌落计数的方法,测定菌株的粘附、侵袭和胞内生长能力。结果杜莫氏军团菌*** TEX-KL、*** NY23和嗜肺军团菌*** philadelphila-1三株细菌在体外生长能力和体内对A549细胞的粘附能力方面无明显差别。*** TEX-KL侵袭进入细胞内的菌数是其他两株菌的1 000倍,差异有统计学意义。结论 *** TEX-KL与*** NY23和嗜肺军团菌*** philadelphila-1相比,对A549细胞具有更高的侵袭力,因此也具有较高的上皮细胞内生长能力。

关键词：杜莫氏军团菌嗜肺军团菌侵袭力细胞内生长能力