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Characteristics and mechanism of Ni2+and Cd2+adsorption by recovered perlite from agar extraction residue

CHINESE JOURNAL OF CHEMICAL ENGINEERING 2024年第8期72卷 141-152页

作者： Wang, Juan zhu, yanbing Jiang, Zedong Du, Xiping Zheng, Mingjing Li, Lijun Ni, Hui Wang, Yuanpeng Li, Zhipeng Li, Qingbiao Jimei Univ Coll Ocean Food & Biol Engn Xiamen 361021 Peoples R China Fujian Prov Key Lab Food Microbiol & Enzyme Engn Xiamen 361021 Peoples R China Res Ctr Food Biotechnol Xiamen City Xiamen 361021 Peoples R China Xiamen Ocean Vocat Coll Xiamen 361012 Peoples R China Xiamen Univ Coll Chem & Chem Engn Xiamen 361021 Peoples R China

Ni2+ and Cd2+ in wastewater accumulated through the ecological chain and could jeopardize human health. Adsorption of Ni2+ and Cd2+ from wastewater using recovered perlite was an important way to solve the problem of resource utilization of solid waste from agar production. Our previous study confirmed that recovered perlite from agar extraction residue had better pore size and specific surface area than commercial perlite. However, the adsorption efficiency and adsorption mechanism of recovered perlite were the main factors limiting its adsorption application. The adsorption process of Ni2+ and Cd2+ by recovered perlite in aqueous solution was described by the pseudo-second-order kinetic equation, and the relevant adsorption mechanism was mainly chemisorption. Compared with commercial perlite, the adsorption removal rate of Ni2+ and Cd2+ by enzymatic recovered perlite could reach 92.9% and 89.2%, respectively, and were improved by 12.63% and 13.03%. Langmuir isothermal adsorption model could better describe the isothermal adsorption process of recovered perlite on heavy metal Ni2+ and Cd2+, and the relevant adsorption mechanism was mainly monolayer adsorption. The X-ray photoelectron spectroscopy (XPS) results indicated that the decrease of Si-O Si2+ hydroxyl coordination bond and the increase of C-Si bond might make the binding effect of recovered perlite with heavy metals stronger. The competitive adsorption of Ni2+ and Cd2+ by recovered perlite was still dominated by chemisorption and monolayer adsorption. This study was expected to provide a theoretical basis and technical support for the removal of Ni2+ and Cd2+ from wastewater using recovered perlite from seaweed residue. (c) 2024 The Chemical Industry and Engineering Society of China, and Chemical Industry Press Co., Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.

关键词： Agar extraction residue Recovered perlite Heavy metal ion Adsorption property HEAVY-METAL IONS AQUEOUS-SOLUTIONS EXPANDED PERLITE DOPED CARBON ADSORPTION REMOVAL ELECTROCOAGULATION COMPOSITES KINETICS FEATURES

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Characteristics and mechanism of Ni2+ and Cd2+ adsorption by recovered perlite from agar extraction residue

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Chinese Journal of Chemical Engineering 2024年第08期 141-152页

作者： Juan Wang yanbing zhu Zedong Jiang Xiping Du Mingjing Zheng Lijun Li Hui Ni Yuanpeng Wang Zhipeng Li Qingbiao Li College of Ocean Food and Biological Engineering Jimei University Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering Research Center of Food Biotechnology of Xiamen City Xiamen Ocean Vocational College College of Chemistry and Chemical Engineering Xiamen University

Ni2+and Cd2+in wastewater accumulated through the ecological chain and could jeopardize human health. Adsorption of Ni2+and Cd2+from wastewater using recovered perlite was an important way to solve the problem of resource utilization of solid waste from agar production. Our previous study confirmed that recovered perlite from agar extraction residue had better pore size and specific surface area than commercial perlite. However, the adsorption efficiency and adsorption mechanism of recovered perlite were the main factors limiting its adsorption application. The adsorption process of Ni2+and Cd2+by recovered perlite in aqueous solution was described by the pseudo-second-order kinetic equation, and the relevant adsorption mechanism was mainly chemisorption. Compared with commercial perlite, the adsorption removal rate of Ni2+and Cd2+by enzymatic recovered perlite could reach 92.9% and 89.2%, respectively, and were improved by 12.63% and 13.03%. Langmuir isothermal adsorption model could better describe the isothermal adsorption process of recovered perlite on heavy metal Ni2+and Cd2+, and the relevant adsorption mechanism was mainly monolayer adsorption. The X-ray photoelectron spectroscopy（XPS） results indicated that the decrease of Si—O Si2+hydroxyl coordination bond and the increase of C—Si bond might make the binding effect of recovered perlite with heavy metals stronger. The competitive adsorption of Ni2+and Cd2+by recovered perlite was still dominated by chemisorption and monolayer adsorption. This study was expected to provide a theoretical basis and technical support for the removal of Ni2+and Cd2+from wastewater using recovered perlite from seaweed residue.

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GeoSLAM激光点云的三维可视化数字影像融合目标细部重建

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激光杂志 2024年第7期45卷 233-239页

作者：朱艳冰郑州科技学院郑州450000

三维激光扫描技术应用范围逐渐扩大,但激光点云缺乏真实的纹理信息,致使目标细部重建与实际目标存在偏差,而三维可视化数字影像技术可以有效地弥补三维激光扫描技术的不足,如何对其进行有效融合应用成为重点研究方向,故提出GeoSLAM激光... 详细信息

三维激光扫描技术应用范围逐渐扩大,但激光点云缺乏真实的纹理信息,致使目标细部重建与实际目标存在偏差,而三维可视化数字影像技术可以有效地弥补三维激光扫描技术的不足,如何对其进行有效融合应用成为重点研究方向,故提出GeoSLAM激光点云的三维可视化数字影像融合目标细部重建方法研究。利用点云滤波算法去除GeoSLAM激光点云中的噪声信息,校正处理三维可视化数字影像的畸变现象,以此为基础,利用Moravec算子提取融合目标细部特征点,配准激光点云与数字影像中的特征点,制定融合目标细部重建程序,从而实现激光点云与数字影像的有效融合,即目标细部的精准重建。实验结果显示:本方法能够更加精准地完成细部重建,相比对比方法而言,具备均值为0.9295的相关系数,和更低的融合目标细部特征点提取错误率,且对建筑类别的数字影像的细部重建效果更好,细部特征点提取错误率仅为1%,充分证实了提出方法应用性能更佳。

关键词：数字影像三维可视化细部重建融合目标 GeoSLAM激光点云

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4种红藻的体外模拟消化酵解特性及其抗炎作用

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中国食品学报 2024年第4期24卷 118-129页

作者：周怡倩郑明静洪涛朱艳冰倪辉姜泽东集美大学海洋食品与生物工程学院福建厦门361021 福建省食品微生物学与酶工程重点实验室福建厦门361021 厦门市食品生物技术研究中心福建厦门361021

目的:通过体外模拟消化酵解和细胞炎症模型探究江蓠、红毛藻、麒麟菜和坛紫菜等4种经济红藻的消化酵解特性及其抗炎潜力,为红藻的高附加值开发提供依据。方法:通过体外模拟口胃肠消化和结肠发酵,测定4种红藻中总糖、还原糖、蛋白质和总... 详细信息

目的:通过体外模拟消化酵解和细胞炎症模型探究江蓠、红毛藻、麒麟菜和坛紫菜等4种经济红藻的消化酵解特性及其抗炎潜力,为红藻的高附加值开发提供依据。方法:通过体外模拟口胃肠消化和结肠发酵,测定4种红藻中总糖、还原糖、蛋白质和总酚的去向。利用16S r RNA基因Illumina MiSeq测序分析红藻对肠道菌群的影响,同时通过高效液相色谱法分析发酵产物中短链脂肪酸的含量,并采用脂多糖诱导的RAW264.7细胞炎症模型评价4种红藻发酵产物的抗炎活性。结果:在口胃肠消化过程中,坛紫菜对碳水化合物、蛋白质及酚类物质具有较好的生物利用度。难以消化的部分到达结肠后充当肠道微生物群的能量来源,其中坛紫菜在发酵过程中产生更多的短链脂肪酸。同时,4种红藻均表现出调节肠道菌群的能力,包括提高拟杆菌门的丰度,降低厚壁菌门/拟杆菌门的比值。此外,红藻发酵液对脂多糖诱导的RAW264.7细胞炎症损伤具有显著抑制作用,红毛藻发酵液具有更好的抑制效果。结论:4种红藻经模拟消化后有较好的生物可及性,能够调节肠道微生物群,同时表现出较好的抗炎潜力,为基于红藻的高值活性产品的开发提供科学依据。

关键词：红藻模拟消化模拟酵解肠道菌群抗炎

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Construction of white spot syndrome virus (WSSV) whole genome phage display library

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Acta Oceanologica Sinica 2007年第2期26卷 75-83页

作者： zhu yanbing YANG Feng School of Biotechnology Jimei University Xiamen 361005 China Third Institute of Oceanography State Oceanic Administration Xiamen 361005 China

A rebuilt vector pCANTAB 5 EE was obtained by inserting a 34 bp double-stranded oligonucleotide which contained a EcoRV recognition sequence into pCANTAB 5 E. White spot syndrome virus （WSSV） genome DNA was fragmented by sonication to isolate fragments mainly in the range of 0.8 ～2.0 kb, then the fragments were blunt-ended with T4 DNA polymerase and cloned into the EcoRV site of pCANTAB 5 EE. The primary recombinant clone of the library was 3.0 × 10^*** PCR of random selected recombinants showed that the size of the inserts was 0.12 ～ 1.77 kb. After the whole library recombinant phages infected Escherichia coli HB2151 cells, the extracellular and periplasmic extracts were dropped on PVDF membranes to perform dot blot, using polyclonal mouse anti-VP24 serum,anti-WSV026 serum,anti-WSV063 serum,anti-WSV069 serum,anti-WSV112 serum, anti WSV238 serum,anti-WSV303 serum and anti-VP26 serum as the primary antibody, respectively. The results showed that the display library could express the viral proteins.

关键词： white spot syndrome virus genome phage display library dot blot

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White spot syndrome virus envelope protein VP124 involved in the virus infection

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Acta Oceanologica Sinica 2008年第4期27卷 130-136页

作者： zhu yanbing WU Chenglin YANG Feng School of Biotechnology Engineering Jimei University Xiamen 361021 China Key Laboratory of Marine Biogenetic Resources Third Institute of Oceanography State Oceanic Administration Xiamen361005 China

White spot syndrome virus （WSSV） is one of the major shrimp pathogens causing large economic losses to shrimp farming. In an attempt to identify the envelope proteins involved in the virus infection, purified WSSV virions were mixed with three antisera against WSSV envelope proteins （VP39, VP124 and VP187 ）, individually. And then they were injected intramuscularly into crayfish （Procambarus clarkii） to conduct in vivo neutralization assays. The results showed that for groups injected with virions only and groups injected with the mixture of virions and antiserum against VP124, the crayfish mortalities were 100% and 60% on the 8th day postinfection, individually. The virus infection could be delayed or neutralized by antibody against the envelope protein VP124. Quantitative PCR was used to further investigate the influence of three antisera described above on the virus infection. The results showed that the antiserum against VP124 could restrain the propagation of WSSV in crayfish. All of the results suggested that the viral envelope protein VP124 played a role in WSSV infection.

关键词： white spot syndrome virus envelope protein VP124 antibody infection neutralization assay

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船用柴油机涡轮辅助EGR系统多参数优化匹配研究

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船舶工程 2024年第2期46卷 64-72页

作者：王广夫祝彦兵张子悦张琨汤旭阳石磊海装沈阳局驻大连地区第一军事代表室辽宁大连116001 上海船舶设备研究所上海200031 上海中船重工船舶推进设备有限公司上海200031 上海交通大学动力机械及工程教育部重点实验室上海200240

为获得柴油机、排气再循环(EGR)系统和涡轮增压系统的耦合优化匹配运行规律,以SC7H两级增压柴油机为研究对象,在GT-Power软件中搭建并校核原机一维模型,并进一步建立了涡轮辅助EGR两级增压柴油机一维模型。针对涡轮辅助EGR与两级增压耦... 详细信息

为获得柴油机、排气再循环(EGR)系统和涡轮增压系统的耦合优化匹配运行规律,以SC7H两级增压柴油机为研究对象,在GT-Power软件中搭建并校核原机一维模型,并进一步建立了涡轮辅助EGR两级增压柴油机一维模型。针对涡轮辅助EGR与两级增压耦合系统的匹配问题,提出了基于过量空气系数λ和EGR率需求的三步骤匹配计算流程。匹配规律显示:高/EGR涡轮匹配流量比和高/低压级压比比基本呈线性关系,涡轮辅助EGR进排气压差随压比比增大先增加后减小,在压比比为1附近取最优,比高压EGR高35.35 kPa,涡轮辅助EGR具有更优的泵气过程。建立全连接神经网络优化代理模型并采用NSGAⅡ算法开展耦合系统多参数多目标优化研究。结果显示:涡轮辅助EGR具有更优的BSFC和NOx排放效果,在TierⅢ的加权NOx排放限值条件下,涡轮辅助EGR可调两级增压系统的全负荷加权油耗比传统高压EGR可调两级增压系统低5.20 g/(kW·h)。

关键词：柴油机废气再循环两级增压匹配方法油耗与排放

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红毛藻多糖抑制大肠杆菌黏附Caco-2细胞的机制

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食品科学 2024年第17期45卷 9-16页

作者：段舒舒郑明静洪涛朱艳冰倪辉姜泽东集美大学海洋食品与生物工程学院福建厦门361021 福建省食品微生物与酶工程重点实验室福建厦门361021 厦门市食品生物工程技术研究中心福建厦门361021

基于体外人结肠腺癌细胞(Caco-2)单层模型,研究红毛藻多糖(Bangia fusco-purpurea polysaccharide,BFP)对大肠杆菌(Escherichia coli)黏附Caco-2细胞的影响以及潜在作用机制。采用CFDA-SE荧光标记技术分析BFP对***黏附Caco-2细胞单层的影响;利用实时聚合酶链式反应分析BFP对Caco-2细胞表面整合素β1和***黏附素fimH及***黏附Caco-2细胞导致细胞产生的炎症因子(白细胞介素(interleukin,IL)-1β、IL-8、肿瘤坏死因子(tumor necrosis factor,TNF)-α)和细胞紧密连接蛋白(闭锁小带蛋白-1(zonula occludens-1,ZO-1)与Occludin)的基因表达情况,最终基于Western Blot分析验证ZO-1、Occludin蛋白表达。结果表明,BFP在质量浓度为400~800μg/mL时能够显著抑制***黏附Caco-2细胞单层;BFP主要通过下调Caco-2细胞表面整合素β1和***黏附素基因fimH的表达抑制细菌的黏附。此外,BFP可显著抑制***及其培养上清液诱导Caco-2细胞产生的炎症细胞因子基因表达的上调、紧密连接蛋白(ZO-1与Occludin)及其基因表达的下调。综上所述,BFP能够抑制***对Caco-2细胞单层的黏附,实验结果可为其开发新型的抗菌产品以及BFP的高值利用和精深加工提供参考。

关键词：红毛藻多糖大肠杆菌 Caco-2细胞细菌黏附肠道稳态

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Purification and biochemical characterization of manganesecontaining superoxide dismutase from deep-sea thermophile Geobacillus sp. EPT3

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Acta Oceanologica Sinica 2014年第12期33卷 163-169页

作者： zhu yanbing LI Hebin NI Hui LIU Jingwen XIAO Anfeng CAI Huinong College of Food and Biological Engineering Jimei University Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering Research Center of Food Microbiology and Enzyme Engineering Technology of Fujian Province Xiamen Medical College

Thermostable SOD is a promising enzyme in biotechnological applications. In the present study, ***3 was isolated from a deep-sea hydrothermal field in the East Pacific. A thermo-stable superoxide dismutase （SOD） from this strain was purified to homogeneity by steps of fractional am-monium sulfate precipitation, DEAE-Sepharose chromatography, and Phenyl-Sepharose chromatography. SOD was purified 13.4 fold to homogeneity with a specific activity of 3 354 U/mg and 11.1% recovery. SOD fromGeobacillussp. EPT3 was of the Mn-SOD type, judged by the insensitivity of the enzyme to both KCN and H2O2. SOD was determined to be a homodimer with monomeric molecular mass of 26.0 kDa. It had high thermostability at 50＆#176;C and 60＆#176;C. At tested conditions,SOD was relatively stable in the presence of some inhibitors and denaturants, such asβ-mercaptoethanol （β-ME）, dithiothreitol （DTT）, phenylmethylsulfonyl fluoride （PMSF）, urea, and guanidine ***. EPT3 SOD showed striking stability across a wide pH range from 5.0 to 11.0. It could withstand denaturants of extremely acidic and alkaline conditions, which makes it useful in the industrial applications.

关键词： Geobacillus sp. superoxide dismutase thermostability purification

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Cloning and characterization of a thermostable carboxylesterase from inshore hot spring thermophile Geobacillus ***1

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Acta Oceanologica Sinica 2012年第6期31卷 117-126页

作者： zhu yanbing LIU Guangming LI Hebin LIU Jingwen BAI Xiaoming CAI Huinong School of Biotechnology Engineering Jimei UniversityXiamen 361021China Research Center of Food Microbiology and Enzyme Engineering Technology(Jimei University)Pujian Province University Xiamen 361021China Research Center of Food Biotechnology of Xiamen City Xiamen 361021China Xiamen Medical College Xiamen 361008China

The gene （741 bp） encoding carboxylesterase from the thermophilic bacterium Geobacillus sp. ZH1 was cloned and overexpressed in Escherichia coll. The purified recombinant protein presented a molecular mass of about 40 kDa by SDS-PAGE analysis. Enzyme assays using p-nitrophenyl esters with different acyl chain lengths as the substrates confirmed its esterase activity, yielding highest specific activity with p-nitrophenyl acetate. Among the p-nitrophenyl esters tested, the carboxylesterase presented preference for p-nitrophenyl caprylate, but hydrolyzed p-nitrophenyl butyrate more efficiently. When p-nitrophenyl butyrate was used as a substrate, the recombinant carboxylesterase exhibited highest activity at pH 8.0 and 60℃. Almost no decrease in esterase activity was observed at 60℃ for 3 h, and over 40% of activity was still maintained after incubation at 90℃ for 3 h. These results indicate that Geobacillus sp. ZH1 recombinant esterase was thermostable. The enzymatic activity was inhibited by the addition of phenylmethylsulfonyl fluoride, indicating that it contains serine residue, which plays a key role in the catalytic mechanism. Except SDS and xylene, this esterase showed stability toward other tested detergents and organic solvents. Cloning, expression, and biochemical characterization of Geobacillus sp. ZH1 carboxylesterase lay a good foundation for its structural characterization and industrial application.

关键词： carboxylesterase expression characterization

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