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From static to dynamic:live observation of the support system after ischemic stroke by two photon-excited fluorescence laser-scanning microscopy

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Neural Regeneration Research 2023年第10期18卷 2093-2107页

作者： Xuan Wu Jia-Rui Li Yu Fu Dan-Yang Chen Hao Nie zhou-ping tang Department of Neurology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Geriatrics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Ischemic stroke is one of the most common causes of mortality and disability ***,treatment efficacy and the progress of research remain *** the critical support system and essential components in neurovascular units,glial cells and blood vessels(including the bloodbrain barrier)together maintain an optimal microenvironment for neuronal *** provide nutrients,regulate neuronal excitability,and prevent harmful substances from entering brain *** highly dynamic networks of this support system play an essential role in ischemic stroke through processes including brain homeostasis,supporting neuronal function,and reacting to ***,most studies have focused on postmortem animals,which inevitably lack critical information about the dynamic changes that occur after ischemic ***,a high-precision technique for research in living animals is urgently ***-photon fluorescence laser-scanning microscopy is a powerful imaging technique that can facilitate live imaging at high spatiotemporal *** fluorescence laser-scanning microscopy can provide images of the whole-cortex vascular 3D structure,information on multicellular component interactions,and provide images of structure and function in the cranial *** technique shifts the existing research paradigm from static to dynamic,from flat to stereoscopic,and from single-cell function to multicellular intercommunication,thus providing direct and reliable evidence to identify the pathophysiological mechanisms following ischemic stroke in an intact *** this review,we discuss exciting findings from research on the support system after ischemic stroke using two-photon fluorescence laser-scanning microscopy,highlighting the importance of dynamic observations of cellular behavior and interactions in the networks of the brain’s support *** show the excellent application prospects and advantages of two-photon fluorescence laser-scanni

关键词： astrocytes blood-brain barrier calcium signaling glymphatic system ischemic stroke microglia network remodel two-photon fluorescence laser-scanning microscopy vessels

Experimental Study of Cell Migration and Functional Differentiation of Transplanted Neural Stem Cells Co-labeled with Superparamagnetic Iron Oxide and Brdu in an Ischemic Rat Model

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Biomedical and Environmental Sciences 2008年第5期21卷 420-424页

作者： WEN-ZHEN ZHU XIANG LI JIAN-PIN QI zhou-ping tang WEI WANG LI WEI AND HAO LEI Department of Radiology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei China Department of Neurology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei China State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics （MRAMP） of Wuhan Institute of Physics and Mathematics （WIPM） of the Chinese Academy of Sciences （CAS） Wuhan 430030 Hubei China

Objective To explore the migration of transplanted neural stem cells co-labeled with superparamagnetic iron oxide （SPIO） and bromodeoxyuridine （Brdu） using the 4.7T MR system and to study the cell differentiation with immuno-histochemical method in ischemic rats. Methods Rat neural stem cells （NSCs） co-labelled with SPIO mediated by poly-L-lysine and bromodeoxyuridine （BrdU） were transplanted into the unaffected side of rat brain with middle cerebral artery occlusion （MCAO）. At weeks 1, 2, 3, 4, 5, and 6 after MCAO, migration of the labelled cells was monitored by MRI. At week 6 the rats were killed and their brain tissue was cut according to the migration site of transplanted ceils indicated by MRI and subjected to Prussian blue staining and immunohistochemical staining to observe the migration and differentiation of the transplanted NSCs. Results Three weeks after transplantation, the linear hypointensity area derived from the migration of labelled NSCs was observed by MRI in the corpus callosum adjacent to the injection site. Six weeks after the transplantation, the linear hypointensity area was moved toward the midline along the corpus callosum. MRI findings were confirmed by Prussian blue staining and immunohistochemical staining of the specimen at week 6 after the transplantation. Flourescence co-labelled immunohistochemical methods demonstrated that the transplanted NSCs could differentiate into astrocytes and neurons. Conclusion MRI can monitor the migration of SPIO-labelled NSCs after transplantation in a dynamical and non-invasive manner. NSCs transplanted into ischemic rats can differentiate into astrocytes and neurons during the process of migration.

关键词： Stem cell transplantation Magnetic resonance imaging Staining and Labelling Cell migration Cell differentiation

MRI Features and Site-specific Factors of Ischemic Changes in White Matter： A Retrospective Study

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Current Medical Science 2018年第2期38卷 318-323页

作者： You-ping ZHANG Na LIU Kai-yan LIU Chao PAN Xuan CAI Shi-qi YANG zhou-ping tang Sha-bei XU Department of Emergency Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China Department of Neurology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China Department of Radiology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China Department of Neurology Luoyang Central Hospital Zhengzhou University Luoyang 471000 China

Brain magnetic resonance imaging （MRI） of the elderly often reveals white matter changes （WMCs） with substantial variability across individuals. Our study was designed to explore MRI features and site-specific factors of ischemic WMCs. Clinical data of consecutive patients diagnosed with ischemic cerebral vascular disease who had undergone brain MRI were collected and analyzed. Multi-logistic regression analysis comparing patients with mild versus severe WMCs was performed to detect independent associations. Analyses of variance （ANOVAs） were used to detect regionally specific differences in lesions. We found that lesion distribution differed significantly across five cerebral areas, with lesions being predominant in the frontal lobe and parieto-occipital area. To explore WMCs risk factors, after adjusting for gender, diabetes mellitus, and hypertension, only age （P〈0.01）, creatinine （P=0.01）, alkaline phosphatase （ALP）（P=0.01） and low-density lipoprotein cholesterol （LDL-C）（P=0.03） were found to be independently associated with severe WMCs. Age （P〈0.001） was strongly associated with WMCs in the frontal lobe while hypertension was independently related to lesions in the basal ganglia （P=0.048） or infratentorial area （P=0.016）. In conclusion, MRI of WMCs showed that ischemic WMCs occurred mostly in the frontal lobe and parieto-occipital area. The infratentorial area was least affected by WMCs. Typically, age-related WMCs were observed in the frontal lobes, while hypertension-related WMCs tended to occur in the basal ganglia and infratentorial area.

关键词： white matter changes ischemic cerebral vascular disease MRI features sitespecific factors

Combined Transplantation of Neural Stem Cells and Olfactory Ensheathing Cells Improves the Motor Function of Rats with Intracerebral Hemorrhage

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Biomedical and Environmental Sciences 2010年第1期23卷 62-67页

作者： zhou-ping tang XUE-WEI XIE YUAN-HONG SHI NA LIU SuI-QIANG ZHU ZAI-WANG LI YUN CHEN Department of Neurology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei China

Objective To investigate the effects of combined transplantation of neural stem cells （NSC） and olfactory ensheathing cells （OEC） on the motor function of rats with intracerebral hemorrhage. Methods In three days after a rat model of caudate nucleus hemorrhage was established, NSCs and OEC, NSC, OEC （from embryos of Wistar rats） or normal saline were injected into bematomas of rats in combined transplantation group, NSC group, OEC group, and control group, respectively. Damage of neural function was scored before and in 3, 7, 14, 30 days after operation. Tissue after transplantation was observed by immunocytochemistry staining. Results The scores for the NSC, OEC and co-transplantation groups were significantly lower in 14 and 30 days after operation than in 3 days after operation （P〈0.05）. The scores for the NSC and OEC groups were significantly lower than those for the control group only in 30 days after operation （P〈0.05）, while the difference for the NSC-OEC group was significant in 14 days after operation （P〈0.05）. Immunocytochemistry staining revealed that the transplanted OEC and NSC could survive, migrate and differentiate into neurons, astrocytes, and oligodendrocytes. The number of neural precursor cells was greater in the NSC and combined transplantation groups than in the control group. The number of neurons differentiated from NSC was significantly greater in the co-transplantation group than in the NSC group. Conclusion Co-transplantation of NSC and OEC can promote the repair of injured tissue and improve the motor fimction of rats with intracerebral hemorrhage.

关键词： Stem cell Olfactory bulb Cell transplantation Intracerebral hemorrhage Motor function

Apolipoprotein E Gene Polymorphisms Are Risk Factors for Spontaneous Intracerebral Hemorrhage:A Systematic Review and Meta-analysis

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Current Medical Science 2019年第1期39卷 111-117页

作者： Hao NIE Yang HU Na LIU ping ZHANG Gai-gai LI Yan-yan LI Chao PAN Hai-han YU Qian WU Guo-feng WU zhou-ping tang Department of Neurology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Affiliated Hospital of Guizhou Medical University Guiyang 550004China

Intracerebral hemorrhage(ICH)is a serious clinical disease with high morbidity,whose pathogenesis might be related to apolipoprotein E(APOE)gene *** comprehensively evaluate the risk factors for ICH occurrence,we performed a *** searched online databases to identify eligible studies based on the relationship between APOE genetic polymorphisms and ICH occurrence *** and pooled odds ratios(ORs)were calculated and by assessing small study bias,we drew the relationship between APOE polymorphisms and ICH *** included 15 eligible studies in our study containing a total of 1642 ICH samples and 5545 normal *** comparison of e4 andε3 APOE genotypes revealed that specific and pooled ORs showed a significantly increased odds ratio in ICH patients with theε4 genotype,indicating thatε4 gene is a risk factor for ICH occurrence,and the heterogeneity is ***,it was found that theε2 genotype also contributed to the incidence rate of ***,after the subgroup analysis by ethnicity,this APOE genetic polymorphism acted as a harmful factor only in white populations,but did not show an effect in Asian *** was suggested that both 82 andε4 APOE alleles were risk factors for ICH in *** were risk factors in white populations only,neither had a detectable effect in Asian populations after subgroup analysing by ethnicity.

关键词： intracerebral hemorrhage apolipoprotein E allele genetic polymorphism metaanalysis

Aggressive Blood Pressure Lowing Therapy in Patients with Acute Intracerebral Hemorrhage is Safe： A Systematic Review and Meta-analysis

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Chinese Medical Journal 2015年第18期128卷 2524-2529页

作者： Chao Pan Yang Hu Na Liu ping Zhang You-ping Zhang Miribanu Aimaiti Hong Deng Ying-Xing tang Feng Xu Sui-Qiang Zhu zhou-ping tang Department of Neurology Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 China

Background： The influence of blood pressure （BP） lowering on intracerebral hemorrhage （ICH） patients is unclear. To assess the safety and efficacy of aggressive antihypertensive therapies in acute ICH patients, we carried out a systematic review and meta-analysis. Methods： PubMed, EMBASE, Cochrane Library, China National Knowledge Infrastructure, and VIP database up to July 2014 were searched. H igh-quality randomized controlled trials were included. Low-quality trials were excluded. Serious adverse events were defined as the primary outcome. The secondary outcomes were hematoma enlargement （HE） at 24 h after onset, mortality, and favorable clinical outcome at 90 days. Results： Four high-quality trials involving a total of 1427 patients met the inclusion criteria and were analyzed. Odds ratios （ORs） of primary outcome was 0.96 （95% confidence interval [CI]： 0.82-1.13, P = 0.61）. ORs of HE at 24 h after onset, mortality and favorable clinical outcome at 90 days were 0.91 （95% CI： 0.72-1.17, P = 0.47）, 0.97 （95% CI： 0.79-1.20, P = 0.81）, 1.13 （95% CI： 0.98-1.30. P = 0.09） respectively. Conclusions： Aggressive BP management policies are safe and might have a potency of reducing HE and improving clinical outcome.

关键词： Antihypertensive Therapy: Blood Pressure: Hematoma Enlargement Hypertension Intracerebral Hemorrhage

P2X7 receptor activation aggravates NADPH oxidase 2-induced oxidative stress after intracerebral hemorrhage

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Neural Regeneration Research 2021年第8期16卷 1582-1591页

作者： Hong Deng Ye Zhang Gai-Gai Li Hai-Han Yu Shuang Bai Guang-Yu Guo Wen-Liang Guo Yang Ma Jia-Hui Wang Na Liu Chao Pan zhou-ping tang Department of Neurology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Endocrinology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Oxidative stress is a crucial pathological process that contributes to secondary injury following intracerebral hemorrhage. P2X7 receptor(P2X7R), which is activated by the abnormal accumulation of extracellular ATP, plays an important role in the regulation of oxidative stress in the central nervous system, although the effects of activated P2X7R-associated oxidative stress after intracerebral hemorrhage remain unclear. Mouse models of intracerebral hemorrhage were established through the stereotactic injection of 0.075 U VII collagenase into the right basal ganglia. The results revealed that P2X7R expression peaked 24 hours after intracerebral hemorrhage, and P2X7R expressed primarily in neurons. The inhibition of P2X7R, using A438079(100 mg/kg, intraperitoneal), reduced nicotinamide adenine dinucleotide phosphate oxidase 2(NOX2) expression and malondialdehyde generation, increased superoxide dismutase and glutathione/oxidized glutathione levels, and alleviated neurological damage, brain edema, and apoptosis after intracellular hemorrhage. The P2X7R inhibitor A438079(100 mg/kg, intraperitoneal injection) inhibited the activation of extracellular signal-regulated kinase 1/2(ERK1/2) and nuclear factor kappa-B(NF-κB) after intracerebral hemorrhage. Blocking ERK1/2 activation, using the ERK1/2 inhibitor U0126(2 μg, intraventricular injection), reduced the level of NOX2-mediated oxidative stress induced by P2X7R activation after intracellular hemorrhage. Similarly, the inhibition of NF-κB, using the NF-κB inhibitor JSH-23(3.5 μg, intraventricular), reduced the level of NOX2-mediated oxidative stress induced by P2X7R activation. Finally, GSK2795039(100 mg/kg, intraperitoneal), a NOX2 antagonist, attenuated P2X7R-mediated oxidative stress, neurological damage, and brain edema after intracerebral hemorrhage. The results indicated that P2X7R activation aggravated NOX2-induced oxidative stress through the activation of the ERK1/2 and NF-κB pathways following intracerebral he

关键词： brain central nervous system factor inflammation injury pathways repair stroke

Ameliorative Effects of Brassinosteroid on Excess Manganese-Induced Oxidative Stress in Zea mays L. Leaves

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Agricultural Sciences in China 2009年第9期8卷 1063-1074页

作者： WANG Hai-hua, FENG Tao, PENG Xi-xu, YAN Ming-li, zhou ping-lan and tang Xin-ke School of Life Sciences, Hunan University of Science and Technology, Xiangtan 411201, P.R.China School of Life Sciences Hunan University of Science and Technology Xiangtan 411201 P.R.China

Manganese （Mn） is becoming an important factor limiting crop growth and yields especially on acid soils. The present study was designed to explore the hypothesis that brassinosteroid application can enhance the tolerance of maize （Zea mays L.） to Mn stress and if so, whether or not the mechanism underlying involves regulation of antioxidative metabolism in leaves. The effects of 24-epibrassinosteroid （EBR） on the growth, photosynthesis, water status, lipid peroxidation, accumulation of reactive oxygen species, and activities or contents of antioxidant defense system in maize plants under Mn stress were investigated by a pot experiment. At supplemented Mn concentrations of 150-750 mg kg^-1 soil, the growth of plants was inhibited in a concentration-dependent manner. The semi-lethal concentration was 550 mg Mn kgq soil. Foliage application with 0.1 mg L^-1 EBR significantly reduced the decrease in dry mass, chlorophyll content, photosynthetic rate, leaf water content, and water potential of plants grown in the soil spiked with 550 mg kg^-1 Mn. The oxidative stress caused by excess Mn, as reflected by the increase in malondialdehyde （MDA） content and lipoxygenase （LOX, EC 1.***.11.12） activity, accumulation of superoxide radical and H2O2, was greatly decreased by EBR treatment. Further investigations revealed that EBR application enhanced the activities of superoxide dismutase （SOD, EC 1.***.1.1）, peroxidase （POD, EC 1.***.1.7）, catalase （EC 1.***.1.6）, ascorbate peroxidase （APX, EC 1.11. 1.11）, dehydroascorbate reductase （DHAR, EC 1.***.5.1）, and glutathione reductase （GR, EC 1.***.4.2）, and the contents of reduced ascorbate and glutathione, compared with the plants without EBR treatment. It is concluded that the ameliorative effects of EBR on Mn toxicity are due to the upregulation of antioxidative capacity in maize under Mn stress.

关键词： excess manganese brassinosteroid oxidative stress antioxidant defense system maize (Zea mays L.)

Insights into Initial Demyelinating Episodes of Central Nervous System during Puerperium

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Chinese Medical Journal 2017年第15期130卷 1791-1795页

作者： Qian Wu Bo Chen Na Liu Yang Hu Chao Pan ping Zhang zhou-ping tang Bi-Tao Bu Department of Neurology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 China

Background：Inflammatory demyelinating disease of central nervous system （CNS） is an inflammatory disease characterized by a high childbearing female ***-related alterations for postpartum demyelinating attacks are not entirely *** study aimed to summarize clinical features of female patients of reproductive age with initial CNS inflammatory demyelinating attacks during ***：Fourteen female patients with initial demyelinating events during puerperium between January 2013 and December 2016 were retrospectively *** of clinical features,neuroimaging,serum antibodies,cerebrospinal fluid （CSF） findings,annualized relapse rate （ARR）,and treatment were ***：Among 14 patients,5 patients were diagnosed with multiple sclerosis （MS）,four as neuromyelitis optica （NMO）,two as longitudinal extensive transverse myelitis,two as clinical isolated syndrome （CIS）,and one as acute brainstem *** the 14 puerperal female patients presented with more than one manifestation of hemiplegia,paraplegia,uroschesis,visual loss or dysarthria,and with mild to moderate abnormalities of *** occurred during the first trimester postpartum and cesarean section was the main delivery way （n =10）.Median Expanded Disability Status Scale （EDSS） scores were 5.0 （range：2.0-9.0） at the onset and 2.5 （range：0-7.0） at the end of *** with MS and CIS had a significantly lower EDSS scores than patients with NMO spectrum disorders （P 〈 0.05）.Median ARR was 0.46 （range：0-1.16）;all patients had a low ARR （0.49 ± 0.34,95% confidence interval：0.29-0.69） with standardized ***：Labor-related alterations in the mother＇s immune system might result in newly-onset demyelinating diseases of central nervous system.

关键词： Cesarean Section Inflammatory Demyelinations Postpartum