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Expression of kallikrein-related peptidase 7 is decreased in prostate cancer

Asian Journal of Andrology 2015年第1期17卷 106-110,I0010页

作者： Chong-Yu Zhang Yu Zhu Wen-Bin Rui jun Dai zhou-jun shen Department of Urology Ruijin Hospital School of Medicine Shanghai Jiaotong University Shanghai China.

Recent evidence suggests that the human kallikrein 7 （KLK7） is differentially regulated in a variety of tumors. The aim of this study was to determine the expression of kallikrein-related peptidase 7 and KLK7 in our large collection of prostate samples. Between August 2000 and December 2012, 116 patients with histologically confirmed prostate cancer （PCa） and 92 with benign prostate hyperplasia （BPH） were recruited into the study. Using immunohistochemistry, quantitative reverse transcription polymerase chain reaction （RT-PCR） and western blot, kallikrein-related peptidase 7 expression in BPH and PCa tissues was determined at the mRNA and protein levels. The relationships between kallikrein-related peptidase 7 mRNA expression and clinicopathological features were analyzed. A total of 64 of 92 （69.57%） benign cases showed positive staining for KLK7 and 23 of 116 （19.83%） malignant cases showed positive, the difference of KLK7 expression between PCa and BPH was statistically significant （P〈 0.001）. The expression level of kallikrein-related peptidase 7 mRNA was significantly decreased in PCa tissues compared with that in BPH tissues and normal prostate tissue. Kallikrein-related peptidase 7 mRNA exhibited different expression patterns in terms of localization depending on pathological category of PCa. Similarly, our western immunoblot analyses demonstrated that the protein expression levels of KLK7 was lower in PCa than in BPH tissues and normal prostate tissue. Kallikrein-related peptidase 7 and KLK7 expression are down-regulated in PCa and lower expression of kallikrein-related peptidase 7 closely correlates with higher Gleason score and higher prostate-specific antigen level.

关键词： human kallikrein 7 kallikrein-related peptidase 7 prostate cancer prostate-specific antigen

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Life-threatening meningitis resulting from transrectal prostate biopsy

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Asian Journal of Andrology 2005年第4期7卷 453-455,页

作者： zhou-jun shen Shan-Wen Chen Hua Wang Xie-Lai zhou Ju-Ping Zhao Department of Urology Ruijin Hospital School of Medicine Shanghai Jiaotong University Shanghai 200025 China Department of Urology First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 China

After antibiotic prophylaxis with metronidazole and levofloxacin, a transrectal sextant biopsy was performed under the guide of transrectal ultrasonography （TRUS） for a 75-year-old suspicious patient with prostate adenocarcinoma. Although antibiotics were also given after this procedure, the patient still developed fever, anxious, agrypnia and headache. Blood cultures remained negative. Lumbar puncture was performed and was consistent with Escherichia coli bacterial meningitis.

关键词： meningitis prostate diagnosis

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Derivatives of Intersection Local Time for Two Independent Symmetricα-stable Processes

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Acta Mathematica Sinica,English Series 2024年第5期40卷 1273-1292页

作者： Huan zhou Guang jun shen Qian YU Department of Mathematics Anhui Normal UniversityWuhu 241002P.R.China Department of Mathematics Nanjing University of Aeronautics and AstronauticsNanjing 211106P.R.China Key Laboratory of Mathematical Modelling and High Performance Computing of Air Vehicles(NUAA) MIITNanjing 211106P.R.China

In this paper,we consider the derivatives of intersection local time for two independent d-dimensional symmetricα-stable processes X^(α) and X^(α)with respective indices α and α.We first study the sufficient condition for the existence of the derivatives,which makes us obtain the exponential integrability and H?lder *** we show that this condition is also necessary for the existence of derivatives of intersection local time at the ***,we also study the power variation of the derivatives.

关键词： Symmetric stable processes intersection local time exponential integrability power variation

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Antitumor effects of human interferon-alpha 2b secreted by recombinant bacillus Calmette-Guérin vaccine on bladder cancer cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第5期13卷 335-341页

作者： Guo-qing DING Yan-lan YU zhou-jun shen Xie-lai zhou Shan-wen CHEN Guo-dong LIAO Yue ZHANG Department of Urology Sir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityHangzhou 310016China Department of Urology Ruijin HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200025China Children's Hospital of Los Angeles Los AngelesCA 90027USA

Objective:Our objective was to construct a recombinant bacillus Calmette-Guérin vaccine(rBCG) that secretes human interferon-alpha 2b(IFNα-2b) and to study its immunogenicity and in vitro antitumor activity against human bladder cancer cell lines T24 and ***:The signal sequence BCG Ag85B and the gene IFNα-2b were amplified from the genome of BCG and human peripheral blood,respectively,by polymerase chain reaction(PCR).The two genes were cloned in Escherichia coli-BCG shuttle-vector pMV261 to obtain a new recombinant plasmid pMV261-Ag85B-IFNα-*** was transformed with the recombinant plasmid by electroporation and designated rBCG-IFNα-*** cells were isolated from human peripheral blood(PBMCs) and stimulated with rBCG-IFNα-2b or wild type BCG for 3 d,and then cultured with human bladder cancer cell lines T24 and *** cytotoxicities were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) ***:BCG was successfully transformed with the recombinant plasmid pMV261-Ag85B-IFNα-2b by electroporation and the recombinant BCG(rBCG-IFNα-2b) was capable of synthesizing and secreting cytokine IFNα-*** proliferation was enhanced significantly by rBCG-IFNα-2b,and the cytotoxicity of PBMCs stimulated by rBCG-IFNα-2b to T24 and T5627 was significantly stronger in comparison to wild type ***:A recombinant BCG,secreting human IFNα-2b(rBCG-IFNα-2b),was constructed successfully and was superior to control wild type BCG in inducing immune responses and enhancing cytotoxicity to human bladder cancer cell lines T24 and *** suggests that rBCG-IFNα-2b could be a promising agent for bladder cancer patients in terms of possible reductions in both clinical dosage and side effects of BCG immunotherapy.

关键词： Bacillus Calmette-Guérin(BCG) vaccine Bladder neoplasms Gene recombination Interferon-alpha 2b

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Preventive effect of gelatinizedly-modified chitosan film on peritoneal adhesion of different types

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World Journal of Gastroenterology 2007年第8期13卷 1262-1267页

作者： Xie-Lai zhou Shan-Wen Chen Guo-Dong Liao zhou-jun shen Zhi-Liang Zhang Li Sun Yi-jun Yu Qiao-Ling Hu Xiao-Dong Jin Department of Urology The First Affiliated Hospital College of Medicine Zhejiang University Hangzhou 310003 Zhejiang Province China Department of Urology Ruijin Hospital School of Medicine Shanghai Jiaotong University Shanghai 200025 China Surgical Department Clinical Medical College of Hangzhou Teachers College Hangzhou 310036 Zhejiang Province China Experimental Center of Medical Science Hangzhou Teachers College Hangzhou 310036 Zhejiang Province China Institute of Polymer Composites Zhejiang University Hangzhou 310027 Zhejiang Province China

AIM： To comparatively study the preventive effect of gelatinizedly-modified chitosan film on peritoneal adhesions induced by four different factors in rats. METHODS： Chitosan was chemically modified by gelatinization, and made into films of 60 μm in thickness, and sterilized. Two hundred Sprague-Dawley rats were randomly divided into five groups, Sham-operation group （group A）, wound-induced adhesion group （group B）, purified talc-induced adhesion group （group C）, vascular ligation-induced adhesion group （group D）, and infection-induced adhesion group （group E）, respectively. In each group, the rats were treated with different adhesion-inducing methods at the cecum of vermiform processes and then were divided into control and experimental subgroups. Serous membrane surface of vermiform processes were covered with the films in the experimental subgroups, and no films were used in the control subgroups. After 2 and 4 wk of treatments, the abdominal cavities were reopened and the adhesive severity was graded blindly according to Bhatia＇s method. The cecum of vermiform processes were resected for hydroxyproline （OHP） measurement and pathological examination. RESULTS： Adhesion severity and OHP level： After 2 and 4 wk of the treatments, in the experimental subgroups, the adhesions were significantly lighter and the OHP levels were significantly lower than those of the control subgroups in group B （2 wk： 0.199 ± 0.026 vs 0.285 ± 0.041 μg/mg pr, P 〈 0.001; 4 wk： 0.183 ± 0.034 vs 0.276 ± 0.03 μg/mg pr, P 〈 0.001）, D （2 wk： 0.216 ± 0.036 vs 0.274 ± 0.040 μg/mg pr, P = 0.004; 4 wk： 0.211 ± 0.044 vs 0.281 ± 0.047 μg/mg pr, P = 0.003） and E （2 wk： 0.259 ± 0.039 vs 0.371 ± 0.040 μg/mg pr, P 〈 0.001; 4 wk： 0.242 ± 0.045 vs 0.355 ± 0.029 μg/mg pr, P 〈 0.001）, but there were no significant differences in groups A （2wk： 0.141 ± 0.028 vs 0.137 =k 0.026 μg/mg pr, P = 0.737; 4 wk： 0.132 ± 0.031 vs 0.150 ± 0.035 μg/mg pr, P = 0.225） and C （2 wk： 0.395 ± 0.044 vs 0.378 ± 0.043 μg/mg p

关键词： Chitosan Gelatinization Chemical modification Peritoneum Adhesion

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SmartEagleEye:A Cloud-Oriented Webshell Detection System Based on Dynamic Gray-Box and Deep Learning

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Tsinghua Science and Technology 2024年第3期29卷 766-783页

作者： Xin Liu Yingli Zhang Qingchen Yu Jiajun Min jun shen Rui zhou Qingguo zhou School of Information Science and Engineering Lanzhou UniversityLanzhou 730000China College of Computer Science and Technology Zhejiang UniversityHangzhou 310058China School of Computing and Information Technology University of WollongongWollongong 2500Australia

Compared with traditional environments,the cloud environment exposes online services to additional vulnerabilities and threats of cyber attacks,and the cyber security of cloud platforms is becoming increasingly prominent.A piece of code,known as a Webshell,is usually uploaded to the target servers to achieve multiple *** Webshell attacks has become a hot spot in current ***,the traditional Webshell detectors are not built for the cloud,making it highly difficult to play a defensive role in the cloud ***,a Webshell detection system based on deep learning that is successfully applied in various scenarios,is proposed in this *** system contains two important components:gray-box and neural network *** gray-box analyzer defines a series of rules and algorithms for extracting static and dynamic behaviors from the code to make the decision *** neural network analyzer transforms suspicious code into Operation Code(OPCODE)sequences,turning the detection task into a classification *** experiment results show that SmartEagleEye achieves an encouraging high detection rate and an acceptable false-positive rate,which indicate its capability to provide good protection for the cloud environment.

关键词： Webshell detection cloud web security deep learning

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Safety and efficacy of levofloxacin versus ciprofloxacin for the treatment of chronic bacterial prostatitis in Chinese patients

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Asian Journal of Andrology 2012年第6期14卷 870-874页

作者： Zhi-Chao Zhang Feng-Shuo Jin Dong-Ming Liu zhou-jun shen Ying-Hao Sun Ying-Lu Guo Andrology Center Peking University F rst Hosp ta nst tute of Urology Peking University Beijing 100034 China Daping Hospital Third Military Medical University Chongqing 400042 China Renji Hospital Shanghai Jiaotong University Shanghai 200001 China Ruijin Hospital Shanghai Jiaotong University Shanghai 200025 China Changhai Hospital Second Military Medical University Shanghai 200433 China)

Levofloxacin is a synthetic fluoroquinolone that is usually used to treat chronic bacterial prostatitis. We investigated the safety and efficacy of levofloxacin compared with ciprofloxacin for the treatment of chronic bacterial prostatitis in Chinese patients. This was a multicenter, open-label, randomized controlled non-inferiority trial. Four hundred and seventy-one patients with clinical symptoms/ signs were enrolled into the study, and 408 patients were microbiologically confirmed chronic bacterial prostatitis, who were randomized to either oral levofloxacin （500 mg q,d.） or ciprofloxacin （500 mg b.i^d.） for 4 weeks. Bacterial clearance rate, clinical symptoms/signs, adverse reactions and disease recurrence were assessed. The clinical symptoms and signs （including dysuria, perineal discomfort or pain） and bacteria cultures in 209 patients treated with levofloxacin and 199 patients treated with ciprofloxacin were similar. The most common bacteria were Escherichia cofiand Staphylococcus aureus. One to four weeks after the end of 4 weeks treatment, the bacterial clearance rate （86.06% vs. 60.03%; P〈O.05） and the clinical efficacy （including clinical cure and clinical improvement（93.30% vs. 71.86%; P〈0.05）） were significantly higher in the levofloxacin-treated group than in the ciprofloxacin-treated group. The microbiological recurrence rate was significantly lower in the levofloxacin-treated group than in the ciprofloxacin-treated group （4.00% vs. 19.25%; P〈0.05）. Rates of adverse events and treatment-related adverse events were slightly lower in the levofloxacin-treated group than in ciprofloxacin-treated group. Levofloxacin showed some advantages over ciprofloxacin in terms of clinical efficacy and disease recurrence, with a low rate of adverse events, for the treatment of chronic bacterial prostatitis in Chinese patients.

关键词： chronic bacterial prostatitis ciprofloxacin levofloxacin recurrence

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Mechanism of microarc oxidation on AZ91D Mg alloy induced byβ-Mg_(17)Al_(12) phase

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International Journal of Minerals,Metallurgy and Materials 2024年第4期31卷 712-724页

作者： Dajun Zhai Xiaoping Li jun shen College of Materials Science and Engineering Chongqing UniversityChongqing 400044China

This work proposed a strategy of indirectly inducing uniform microarc discharge by controlling the content and distribution ofβ-Mg_(17)Al_(12)phase in AZ91D Mg *** kinds of nano-particles(ZrO_(2)and TiO_(2))were designed to be added into the substrate of Mg alloy by friction stir processing(FSP).Then,Mg alloy sample designed with different precipitated morphology ofβ-Mg_(17)Al_(12)phase was treated by microarc oxidation(MAO)in Na_(3)PO_(4)/*** characteristics and performance of the MAO coating was analyzed using scanning electron microscopy(SEM),energy dispersive spectrometer(EDS),X-ray diffraction(XRD),X-ray photoelectron spectroscopy(XPS),contact angle meter,and potentiodynamic *** was found that the coarseα-Mg grains in extruded AZ91D Mg alloy were refined by FSP,and theβ-Mg_(17)Al_(12)phase with reticular structure was broken and *** nano-ZrO_(2)particles were pinned at the grain boundary by FSP,which refined theα-Mg grain and promoted the precipitation ofβ-Mg_(17)Al_(12)phase in *** effectively inhibited the“cascade”phenomenon of microarcs,which induced the uniform distribution of discharge *** MAO coating on Zr-FSP sample had good wettability and corrosion ***,TiO_(2)particles were hardly detected in the coating on TiFSP sample.

关键词： AZ91D Mg alloy microarc oxidation friction stir processing ZrO_(2) TiO_(2) β-Mg_(17)Al_(12)

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Study of primordial deuterium abundance in Big Bang nucleosynthesis

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Nuclear Science and Techniques 2024年第3期35卷 208-215页

作者： Zhi-Lin shen Jian-jun He Key Laboratory of Beam Technology of Ministry of Education College of Nuclear Science and TechnologyBeijing Normal UniversityBeijing 100875China Institute of Radiation Technology Beijing Academy of Science and TechnologyBeijing 100875China

Big Bang nucleosynthesis(BBN)theory predicts the primordial abundances of the light elements^(2) H(referred to as deuterium,or D for short),^(3)He,^(4)He,and^(7) Li produced in the early *** these,deuterium,the first nuclide produced by BBN,is a key primordial material for subsequent *** date,the uncertainty in predicted deuterium abundance(D/H)remains larger than the observational *** this study,the Monte Carlo simulation code PRIMAT was used to investigate the sensitivity of 11 important BBN reactions to deuterium *** found that the reaction rate uncertainties of the four reactions d(d,n)^(3)He,d(d,p)t,d(p,γ)^(3)He,and p(n,γ)d had the largest influence on the calculated D/H ***,the calculated D/H uncertainty cannot reach observational precision even with the recent LUNA precise d(p,γ)^(3) He *** the nuclear physics aspect,there is still room to largely reduce the reaction-rate uncertainties;hence,further measurements of the important reactions involved in BBN are still necessary.A photodisintegration experiment will be conducted at the Shanghai Laser Electron Gamma Source Facility to precisely study the deuterium production reaction of p(n,γ)d.

关键词： Big Bang nucleosynthesis Abundance of deuterium Reaction cross section Reaction rate Monte Carlo method

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An improved installation of 2-hydroxy-4-methoxybenzyl(iHmb)method for chemical protein synthesis

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Chinese Chemical Letters 2024年第5期35卷 145-150页

作者： Ying Li Long-Jie Wang Yong-Kang zhou jun Liang Bin Xiao Ji-shen Zheng The First Affiliated Hospital of USTC MOE Key Laboratory for Membraneless Organelles and Cellular DynamicsDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China Department of Chemistry University of Science and Technology of ChinaHefei 230027China

The 2-hydroxy-4-methoxybenzyl(Hmb)backbone modification can prevent amide bond-mediated sidereactions(e.g.,aspartimide formation,peptide aggregation)by installing the removable Hmb group into a peptide bond,thus improving the synthesis of long and challenging peptides and ***,its use is largely precluded by the limited Hmb’s installation *** this report,an improved installation of Hmb(iHmb)method was developed to achieve the flexible installation and the convenient removal of *** iHmb method involves two critical steps:(1)oxidative diazotization of the readily installed 2-hydroxy-4-methoxy-5-amino-benzyl(Hmab)to give 2-hydroxy-4-methoxy-5-diazonium-benzyl(Hmdab)by combining soamyl nitrite(IAN)/HBF_(4),and(2)reductive elimination of Hmdab to give the desired Hmb by 1,2-ethanedithiol(EDT).The iHmb method enables the installation of Hmb at any primary amino acid including the highly sterically hindered amino acids(e.g.,valine and isoleucine).The practicality and utility of the iHmb method was demonstrated by one-shot solid-phase synthesis of a challenging aspartimide-prone peptide,the mirror-image version of a hydrophobic peptide and a long-chain peptide up to ***,the iHmb method can be utilized to facilitate chemical protein ligation,as exemplified by the synthesis of the single-spanning membrane protein *** iHmb method expands the toolkit for peptide synthesis and ligation and facilitates the preparation of peptides/proteins.

关键词： Chemical protein synthesis Solid-phase peptide synthesis Removable backbone modification 2-Hydroxy-4-methoxybenzyl(Hmb)group Aspartimide-prone peptides Difficult peptides Membrane proteins

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