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Epidemiology of toxoplasmosis: role of the tick Haemaphysalis longicornis

Infectious Diseases of Poverty 2016年第1期5卷 120-125页

作者： Yongzhi zhou Houshuang Zhang Jie Cao Haiyan Gong jinlin zhou Key Laboratory of Animal Parasitology of Ministry of Agriculture Shanghai Veterinary Research InstituteChinese Academy of Agricultural SciencesShanghai 200241China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou 225009China

Background:Toxoplasma gondii infection is mainly caused by ingestion of water or food that is contaminated with oocysts excreted by cats,or by eating raw meat containing *** tissue ***,oral transmission does not explain the common occurrence of toxoplasmosis in a variety of hosts,such as herbivorous animals,birds,and wild *** information exists on the maintenance of *** parasites in nature and routes of transmission to domestic and wild animal ***,this study evaluated the role of Haemaphysalis longicornis ticks in the epidemiology of ***:The real-time polymerase chain reaction(qPCR)technique was used to detect the presence of *** DNA in ticks collected from the *** observe the amount of dynamic changes of *** in the tick’s body and its infectivity,microinjection of green fluorescence parasites was *** laboratory conditions,we evaluated if *** ticks were infected with *** and their potential to transmit the infection to other hosts using traditional parasitological methods coupled with molecular detection ***:The infection rates of *** parasites among field-collected adult and nymph *** ticks were 11.26%and 5.95%,*** can survive and remain infective in a tick’s body for at least 15 *** found that blood feeding of infected ticks did not transmit *** to hosts,however,ingestion of infected ticks may be a transmission route between ticks and other common ***:The *** infection in ticks could serve as a reservoir for toxoplasmosis transmission.

关键词： Tick Haemaphysalis longicornis Toxoplasma gondii Transmission Epidemiology

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Operation conditions design based on the qualitative and quantitative analysis for glycosylation reactions 36

Operation conditions design based on the qualitative and qua...

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第36届中国控制会议

作者： Yuhan Nan Jing Wang jinlin zhou Long Cheng College of Information Science and Technology Beijing University of Chemical Technology State key laboratory of management and control for complex systems Institute of AutomationChinese Academy of Sciences

ISBN: (纸本)9781538629185

Glycosylation reactions play a significant role on the targeted therapy of monoclonal antibody *** this paper,a new method implemented by combination of qualitative analysis and quantitative analysis is introduced to optimize the operating conditions of glycosylation reaction ***,gain matrix based on ANOVA design is presented to evaluate the relationship between reaction output（different glycan classes） and system input（glycosylation enzymes）.Due to the high dimension and high nonlinearity of glycosylation reactions,the singular value decomposition of process gain matrix is adopted to qualitatively analyze the effect of glycosylation enzymes in order to find the appropriate operation ***,genetic algorithm is used to quantitatively optimize the operation conditions for desired target of monoclonal antibody *** quantitative operation conditions are consistent with the reaction mechanism and qualitative analysis results of process gain.

关键词： Operating Conditions Optimization Gain Matrix Genetic Algorithm

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Hydrogen-bond locked purine chromophores with high photostability for lipid droplets imaging in cells and tissues

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Chinese Chemical Letters 2023年第4期34卷 300-303页

作者： jinlin zhou Kun Li Lei Shi Hong Zhang Haoyuan Wang Yimin Shan Shanyong Chen Xiao-Qi Yu Key Laboratory of Green Chemistry and Technology Ministry of EducationCollege of ChemistrySichuan UniversityChengdu 610064China State Key Laboratory of Oral Diseases West China Hospital of StomatologySichuan UniversityChengdu 610064China Department of Chemistry Xihua UniversityChengdu 610039China

Recently, hydrogen-bonding has attracted extensive attention in the design of chromophores. Here, a new class of hydrogen-bond locked purine chromophores(HOPs) were reported by introducing a hydroxyphenyl group into the C(6) position of purine. The intramolecular hydrogen bond plays a dominant role to light up these probes. As a bonus, HOPs show high photostability. Moreover, HOPs exhibit remarkable capability for the specific lipid droplets imaging in living cells with excellent biocompatibility and are also potential for diagnosing fatty liver diseases. These results bring important new insights into the photophysics of the purine-based chromophores and provide a new scaffold with high photostability for bioimaging.

关键词： Fluorescent probe Purine Hydrogen bond Lipid droplets Fatty liver

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EFFECTS OF ACUTE EXERCISE ON THE Ca^(2+) TRANSPORT CAPACITY OF SARCOPLASMIC RETICULUM OF DIFFERENT SKELETAL MUSCLE FIBER TYPES IN RATS

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北京体育大学学报 1996年第S2期19卷 88-92页

作者： TIAN YE LI JIE zhou jinlin(Beijing University of Physical Education)

This paper studied the changes of SR Ca2+transport capacity of different skeletal muscle fiber types in rats immediately after they ran on a motor-driven treadmill at 18 m/min （100 mins）. The results showed that SR Ca2+-ATPase activity of slow-twitch and fast-twitch fibers decreased by 83. 32% （P＞0.01） and 12．18％（P＜0. 05） respectively in comparison with the values of control group. Moreover,there were obvious reductions in both the iultial rate of SR Ca2+ uptake and .the maximal capacity of SR Ca2+ uptake in slow -twitch fibers after running. These findings suggested that the decreases of the SR Ca2+ transport capacity of different muscle fiber types might be related to the exertise-induced fatigue of skeletal muscles.

关键词： Ca2+ transport capacity sarcoplasmic reticulum

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Estimation for Jump-diffusion Term Structure of Interest Rate for China Government Bond Market

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Journal of Systems Science and Information 2006年第1期4卷 67-71页

作者： Li zhou jinlin Li Junfeng Li School of Management and Economics Beijing Institute of Technology Beijing 100081 China

This paper examines the term structure of interest rate empirically, and discovers that jump-diffusion process is better than pure diffusion process when describing the stochastic behavior of interest rate, which including jump risk. Using two-stage method to estimate the term structure of China government bond market. Fitting the initial term structure with B-spline approximation method, and then as input to jump-diffusion model parameter estimation. The result accounts for that term structure with jump can explain the actual conditions of China government bond market.

关键词： term structure of interest rate jump-diffusion B-spline approximation

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Epidemiology of Toxoplasmosis:Role of the Tick Haemaphysalis longicornis

Epidemiology of Toxoplasmosis:Role of the Tick Haemaphysalis...

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中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会

Toxoplasmosis is a widespread zoonotic disease caused by the parasitic protozoan Toxoplasma *** of *** in nature and modes of transmission to warm-blooded hosts are poorly *** study evaluated the role of ticks in the epidemiology of *** analysis demonstrated the presence of *** DNA in the hard tick,Haemaphysalis longicornis,at frequencies of11.25%and 5.95%in adults and nymphs *** dynamic amount changes of *** in the tick body and its infectivity were studied by microinjection of green fluorescence *** showed the *** can survive and remain infective in the tick body for at least 15 *** laboratory conditions,ticks were infected with *** after taking blood from infected mammal *** feeding of infected ticks did not transmit *** to hosts;however,ingestion of infected ticks may be a route of *** transmission between ticks and other common *** infection in ticks could serve as a reservoir for toxoplasmosis transmission.

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ATG5 is instrumental in the transition from autophagy to apoptosis during the degeneration of tick salivary glands

ATG5 is instrumental in the transition from autophagy to apo...

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中国畜牧兽医学会兽医寄生虫学分会第一届青年科学家学术论坛

作者： Yanan Wang Houshuang Zhang Li Luo Yongzhi zhou Jie Cao jinlin zhou Key Laboratory of Animal Parasitology of Ministry of Agriculture Shanghai Veterinary Research InstituteChinese Academy of Agricultural Sciences

Female tick salivary glands undergo rapid degeneration several days post *** degeneration may be caused by the increased concentration of ecdysone in the hemolymph during the fast feeding period and both autophagy and apoptosis *** this work,we first proved ATG and Caspase gene expression peaks during degeneration of the tick salivary *** explored the regulatory role of Rhipicephalus haemaphysaloides autophagy related 5(RhATG5) in the degeneration of tick salivary *** the fast feeding phase,RhATG5 was cleaved and both calcium concentration and the transcription of RhCalpains increased in the salivary glands Recombinant RhATG5 was cleaved by μ-Calpain only in the presence of calcium;the mutant RhATG5 was not *** with 20-hydroxyecdysone(20 E) led to programmed cell death in the salivary glands of unfed ticks in vitro,RhATG8-phosphatidylethanolamine(PE)was upregulated in ticks treated with low concentration of 20 ***,RhATG8-PE decreased and RhCaspase-7 increased in ticks treated with a high concentration of 20 E and transformed autophagy to *** concentrations of 20 E led to the cleavage of *** concentration and expression of RhCalpains were also upregulated in the tick salivary *** interference(RNAi) of RhATG5 in vitro inhibited both autophagy and apoptosis of the tick salivary *** of RhATG5 in vivo significantly inhibited the normal feeding *** results demonstrated that high concentrations of 20 E led to the cleavage of RhATG5 by increasing the concentration of calcium and stimulated the transition from autophagy to apoptosis.

关键词： tick salivary gland degeneration ATG5 autophagy apoptosis

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A cysteine proteinase from Babesia microti may be the potential targets for drug development

A cysteine proteinase from Babesia microti may be the potent...

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第十一届全国寄生虫学青年工作者学术研讨会

作者： Haiyan Gong Yanfang Du Yongzhi zhou Houshuang Zhang Jie Cao jinlin zhou Shanghai Veterinary Research Institute

Cysteine proteinase from Plasmodium is demonstrated to attend hydrolysis of hemoglobin as well as the invasion and egress of the parasite in red blood cells,thus is considered as the target for drug *** order to explore the function of similar molecules from Babesia microti,a cysteine proteinase(Bm CYP) was isolated in the present study,with a full length of 1418 *** open reading frame(ORF) of 1338 bp assumed to encode 445 amino acids,with a molecular size of 49.7 k *** predicted protein sequence showed the highest homology to *** recombinant protein r Bm CYP/His demonstrated enzymatic activity to the substrate on a dose dependent ***,this activity was inhibited by a cystatin from Rhipicephalus haemaphysaloides(Rhcyst-1).During the feeding period of ticks on the ***-infected mice,Rhcyst-1 m RNA was up-regulated until the tick got engorged,then the expression *** may suggest the interaction between ticks and ***-1 showed inhibitive activity to cathepsin B,C,L and S,but had impact on neither normal cell nor normal *** may hint its potential as anti-Babesia drug,which will be investigated in the future.

关键词： bmcyp rhcyst-1 inhibition activity

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Seroprevalence survey of canine Babesia gibsoni infection and tick species in dogs in East China

Seroprevalence survey of canine Babesia gibsoni infection an...

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中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会

作者： Jie Cao Qiqing Yang Jianwei Zhang Yongzhi zhou Houshuang Zhang Haiyan Gong jinlin zhou Key Laboratory of Animal Parasitology of Ministry of Agriculture Shanghai Veterinary Research InstituteChinese Academy of Agricultural Sciences Merial International Trading(Shanghai) Co. Ltd.

A seroprevalence survey of canine Babesia gibsoni infection in East China was conducted using an ELISA with recombinant *** thrombospondin-related adhesive protein(BgTRAP).A total of 1170 dogs from East China were examined and the seroprevalence was 9.23%.The proportion of samples was81.2%,7.86%and 10.94%from pet,working and fighting dogs,*** fighting dogs showed highest seroprevalence(39.8%) compared with working dogs(26.1%) and pet dogs(3.47%).These results indicate that *** infection of dogs has a widespread geographic distribution throughout East China.A history of tick infestation and bites might be an important factor in *** dominant ticks collected from the dogs were identified as Rhipicephalus sanguineus(65.57%),Haemaphysalis longicornis(21.58%) and Rhipicephalus hemaphysaloides(10.7%).Besides adult,larval and nymph stages of ticks were also recorded on *** is the first report of seroprevalence of canine *** infection and tick species in dogs in China.

关键词： Seroprevalence Babesia gibsoni dog tick

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RT-PCR法分离伊氏锥虫VSG基因的研究

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畜牧兽医学报 2000年第3期31卷 277-280页

作者：周金林沈杰中国农科院上海家畜寄生虫病研究所上海200232

应用异硫氰酸胍一酚一氯仿一步法分离伊氏锥虫安徽株克隆虫体ShTatl的二个抗原变异体ShTat1 3和ShTat1 5总RNA ,在含有甲醛的琼脂糖凝胶电泳后 ,转印到硝酸纤维膜上。根据布氏锥虫VSGmRNA3’端保守序列 ,设计合成了一个互补于它的含十... 详细信息

应用异硫氰酸胍一酚一氯仿一步法分离伊氏锥虫安徽株克隆虫体ShTatl的二个抗原变异体ShTat1 3和ShTat1 5总RNA ,在含有甲醛的琼脂糖凝胶电泳后 ,转印到硝酸纤维膜上。根据布氏锥虫VSGmRNA3’端保守序列 ,设计合成了一个互补于它的含十八个碱基的寡核苷酸 5’—GGTGTTAAAATATATCAG— 3’ ,经32 P标记 ,Northern杂交 ,首次证明伊氏锥虫含有同样的保守序列。利用合成的十八碱基寡核苷酸作为反转录特异引物 ,成功合成cDNA第一链 ,以cDNA第一链作为PCR扩增模板 ,均能特异性的扩增出一条主带为 1 5kb的DNA ,与VSG基因大小相符。根据所有锥虫mRNA在 5’端的保守序列 ,设计合成了RT -PCR下游引物 5’—GAACAGTTTCTG TACTATATTG— 3’。对伊氏锥虫安徽株克隆虫体的二个变体ShTat1 3和ShTat1 5的RNA ,进行RT—PCR扩增 ,均特异性地分离出VSG基因 ,二个变异体VSG基因大小差异显著 ,其中ShTat1 3的VSG基因为 1 7kb ,而ShTat1 5的VSG基因则为 1 4kb。

关键词：伊氏锥虫 VSG基因分离 RT-PCR 抗原变异

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