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地下生态粮仓应用要点分析

粮食与饲料工业 2024年第4期 24-26页

作者：周俊梅涂旭刘震张朝富国粮武汉科学研究设计院有限公司湖北武汉430079

从地下生态粮仓建设、进出仓工艺和储粮工艺三个方面,分析了地下生态粮仓的优势、重难点与应用情况,提出了地下生态粮仓研究亟待解决的问题,为地下生态粮仓的合理建设提供参考,以期促进地下生态粮仓的研究、推广和应用。

关键词：地下生态粮仓仓房建设进出仓工艺粮食储藏

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浅谈BIM技术在粮油工程设计中的优势、特点和展望

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粮食与饲料工业 2024年第4期 7-10页

作者：周娜鲁艳秋郜培官雪薇钟华周俊梅国粮武汉科学研究设计院有限公司湖北武汉430079

为应对粮油工程设计日趋激烈的竞争,寻求一种新的工程项目设计方式及管理模式日益迫切。BIM技术作为一种基于数字化建筑信息模型的技术,因其具有可视性、模拟性、协调性、优化性、可出图性等特点,已由国内建筑行业推广到粮油、食品等多... 详细信息

为应对粮油工程设计日趋激烈的竞争,寻求一种新的工程项目设计方式及管理模式日益迫切。BIM技术作为一种基于数字化建筑信息模型的技术,因其具有可视性、模拟性、协调性、优化性、可出图性等特点,已由国内建筑行业推广到粮油、食品等多个行业。通过简述BIM技术在粮油工程设计中的优势、特点和未来发展展望,旨在为在粮油工程设计中推广应用BIM技术提供参考。

关键词： BIM技术粮油工程三维数字模型项目设计

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Host-Induced Gene Silencing of Effector AGLIP1 Enhanced Resistance of Rice to Rhizoctonia solani AG1-IA

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Rice science 2024年第4期31卷 463-474,I0031-I0034页

作者： ZHAO mei LIU Xiaoxue WAN jun zhou Erxun SHU Canwei Pesticide State Key Laboratory of Green Pesticide Guangdong Province Key Laboratory of Microbial Signals and Disease ControlCollege of Plant ProtectionSouth China Agricultural UniversityGuangzhou 510642China College of Life Science and Engineering Henan University of Urban ConstructionPingdingshan 467036China

Rice sheath blight, caused by Rhizoctonia solani AG1-IA, is a major disease in rice-growing areas worldwide. Effectors of phytopathogenic fungi play important roles during the infection process of fungal pathogens onto their host plants. However, the molecular mechanisms by which R. solani effectors regulate rice immunity are not well understood. Through prediction, 78 candidate effector molecules were identified. Using the tobacco rattle virus-host induced gene silencing(TRV-HIGS) system, 45 RNAi constructs of effector genes were infiltrated into Nicotiana benthamiana leaves. The results revealed that eight of these constructs resulted in a significant reduction in necrosis caused by infection with the AG1-IA strain GD-118. Additionally, stable rice transformants carrying the double-stranded RNA construct for one of the effector genes, AGLIP1, were generated to further verify the function of this gene. The suppression of the AGLIP1 gene increased the resistance of both N. benthamiana and rice against GD-118, and also affected the growth rate of GD-118, indicating that AGLIP1 is a key pathogenic factor. Small RNA sequencing showed that the HIGS vectors were processed into si RNAs within the plants and then translocated to the fungi, leading to the silencing of the target genes. As a result, AGLIP1 might be an excellent candidate for HIGS, thereby enhancing crop resistance against the pathogen and contributing to the control of R. solani infection.

关键词： Rhizoctonia solani host-induced gene silencing lipase tobacco rattle virus

超声造影联合肿瘤自身抗体检测对早期肺癌的诊断价值

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中国医疗器械信息 2024年第10期30卷 33-35页

作者：周美君王健肖雅丽唐琼谭卫龙湘南学院附属医院湖南郴州423000

目的:研讨超声造影定量分析联合7项肿瘤自身抗体检测对早期肺癌的诊断价值。方法:收集本院自2021年10月~2023年10月60例早期肺癌患者为研究对象,以病理诊断为金标准,分析超声造影定量分析、7项肿瘤自身抗体检测、超声造影定量分析联合7... 详细信息

目的:研讨超声造影定量分析联合7项肿瘤自身抗体检测对早期肺癌的诊断价值。方法:收集本院自2021年10月~2023年10月60例早期肺癌患者为研究对象,以病理诊断为金标准,分析超声造影定量分析、7项肿瘤自身抗体检测、超声造影定量分析联合7项肿瘤自身抗体检测诊断价值。结果:以病理结果为金标准,超声造影定量分析和7项肿瘤自身抗体检测与病理结果之间均有显著差异。联合检测诊断优于超声造影定量分析和7项肿瘤自身抗体检测,更符合金标准;联合应用诊断的敏感度和准确性均显著高于超声造影定量分析和7项肿瘤自身抗体检测,指标对比明显,P<0.01;联合诊断截断值、敏感性最高,特异性最低,曲线下面积最高,与超声造影定量分析和7项肿瘤自身抗体检测有显著差异,P<0.05。结论:超声造影定量分析联合7项肿瘤自身抗体检测用于肺癌的早期诊断具有临床应用价值。

关键词：超声造影定量分析肿瘤自身抗体联合诊断早期肺癌

Study on Burning Temperature Versus Time Graph of Moxa Sticks in a Novel Animal Moxibustion Device

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Journal of Acupuncture and Tuina Science 2014年第3期12卷 194-198页

作者： zhou jun-mei Wei Fang Liu Su-jun Zhang Bi-meng Han Chou-ping Department of Acupuncture and Moxibustion Shanghai First People's Hospital Shanghai Jiaotong University School of Medicine Department of Central Lab Shanghai First People's HospitalShanghai Jiaotong University School of Medicine

Objective: To invent a novel animal experimentation moxa device and intend to solve temperature fluctuations during moxa stick burning in animal moxibustion test. Methods: Smokeless moxa sticks of 4 mm in diameter and 120 mm in length were selected in this trial. The target temperature was(47±1) ℃. The burning temperature versus time graph of moxa sticks during un-interfered time period were recorded when the distances between moxa sticks and thermometer were 2 mm, 3 mm and 4 mm respectively. In addition, the total lengths of burned moxa stick ashes within 0-5 min were recorded. Then the moxa stick burning temperature versus time graph after intervention was measured. Results: The maximum temperatures during test duration when the distances were 2 mm, 3 mm and 4 mm were(47.37±0.25) ℃,(42.62±2.05) ℃ and(35.50±0.40) ℃respectively the total lengths of burned ashes from 0-5 min were(5.1±0.08) mm,(12.17±0.44) mm,(14.8±0.31) mm,(17.25±0.17) mm and(19.82±0.67) mm respectively with a distance of 2 mm between a moxa stick and the testing area, remove 5 mm ashes every 2 min, then the maximum and minimum temperatures within 10 min were(48.37±0.47) ℃and(47.12±0.25) ℃, showing no statistical significance(P=0.12). Conclusion: The novel animal experimentation moxa device can help to maintain relatively constant moxa stick burning temperature during test period.

关键词： Moxibustion Therapy Moxa Stick Moxibustion Animal Experimentation Instrumentation

Induction of virus-neutralizing antibodies and T cell responses by dengue virus type 1 virus-like particles prepared from Pichia pastoris

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Chinese Medical Journal 2012年第11期125卷 1986-1992页

作者： TANG Yun-xia JIANG Li-fang zhou jun-mei YIN Yue YANG Xiao-meng LIU Wen-quan FANG Dan-yun Department of Microbiology Zhongshan School of MedicineSun Yat-sen UniversityGuangzhouGuangdong 510080China

Background Dengue is currently a significant global health problem but no vaccines are available against the four dengue serotypes virus infections. The development of safe and effective vaccines has been hampered by the requirement of conferring complete protection against all four dengue serotypes and the lack of a convenient animal model. Virus-like particles （VLPs） have emerged as a promising subunit vaccine candidate. One strategy of vaccine development is to produce a tetravalent dengue subunit vaccine by mixing recombinant VLPs, corresponding to all four dengue virus serotypes. Towards this end, this study aimed to establish a Pichia pastoris （P. pastoris） expression system for production of dengue virus type 1 （DENV-1） VLPs and evaluate the humoral and cellular immune response of this particle in mice. Methods A recombinant yeast P. pastoris clone containing prM and E genes of DENV-1 was constructed and DENV-1 VLPs expressed by this clone were analyzed by sucrose density gradient centrifugation, Western blotting, and transmission electron microscope. Groups of mice were immunized by these particles plus adjuvant formulations, then mice were tested by ELISA and neutralization assay for humoral immune response, and by lymphocyte proliferation and cytokine production assays for a cellular immune response. Results Our data demonstrated that recombinant DENV-1 VLPs consisting of prM and E protein were successfully expressed in the yeast P. pastoris. Sera of VLPs immunized mice were shown to contain a high-titer of antibodies and the neutralization assay suggested that those antibodies neutralized virus infection in vitro. Data from the T lymphocyte proliferation assay showed proliferation of T cell, and ELISA found elevated secretion levels of interferon IFN-γ and IL-4. Conclusions P. pastoris-expressed DENV-1 VLPs can induce virus neutralizing antibodies and T cell responses in immunized mice. Using P. pastoris to produce VLPs offers a promising and economic str

关键词： dengue virus virus-like particles Pichia pastoris

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蛋白质的错误折叠与疾病

生物化学与生物物理进展 2000年第6期27卷 579-584页

作者：周筠梅中国科学院生物物理研究所

蛋白质是生物体内一切功能的执行者 .人体内的任何功能 ,从催化化学反应到抵御外来侵略都是蛋白质作用的结果 .蛋白质折叠是生命活动的最基本过程 ,近年发现蛋白质的错误折叠可以导致一些疾病 .蛋白质的错误折叠与疾病的关系已成为分子... 详细信息

蛋白质是生物体内一切功能的执行者 .人体内的任何功能 ,从催化化学反应到抵御外来侵略都是蛋白质作用的结果 .蛋白质折叠是生命活动的最基本过程 ,近年发现蛋白质的错误折叠可以导致一些疾病 .蛋白质的错误折叠与疾病的关系已成为分子生物学新的研究前沿 .介绍了细胞内保证蛋白质正常功能的“质量控制”系统 ,重点讨论了翻译后的质量控制。

关键词：蛋白质错误折叠蛋白传染子疾病治疗试剂

Clinical Study on Acupoint Injection plus Acupuncture for Insomnia Due to Heart-kidney Disharmony

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Journal of Acupuncture and Tuina Science 2014年第4期12卷 225-229页

作者： Zheng Qiao-ping Zhang Bi-meng zhou jun-mei Hu Qi-long Hong Jue Branch of First People’s Hospital of Shanghai Shanghai First People’s Hospital Shanghai Jiaotong University School of Medicine Shanghai Yangzhi Rehabilitation Hospital

Objective: To observe the clinical efficacy of acupoint injection at Neiguan(PC 6) plus acupuncture in treating insomnia due to heart-kidney disharmony. Methods: A hundred patients with insomnia due to heart-kidney disharmony were randomized into an observation group and a control group. Fifty-three cases in the observation group were intervened by acupoint injection at Neiguan(PC 6) plus acupuncture; while 54 cases in the control group were intervened by acupuncture alone. The Pittsburgh sleep quality index(PSQI) was evaluated before and after intervention, and the therapeutic efficacies of the two groups were compared. Results: Acupoint injection at Neiguan(PC 6) plus acupuncture produced significantly higher efficacies in improving sleep quality, shortening sleep latency, and enhancing sleep efficiency than acupuncture alone(P<0.05).Conclusion: The two groups both can improve the condition of insomnia; acupoint injection at Neiguan(PC 6) has significant advantages, manifested by a higher therapeutic efficacy for insomnia due to heart-kidney disharmony, higher safety evaluation, efficiency, and less adverse events, thus proper for clinical application.

关键词： Point, Neiguan (PC 6) Hydro-acupuncture Acupuncture Therapy Non-interaction Between the Heart and Kidney Insomnia

Prediction and analysis of albumin-bilirubin score combined with liver function index and carcinoembryonic antigen on liver metastasis of colorectal cancer

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World Journal of Gastrointestinal Surgery 2024年第6期16卷 1670-1680页

作者： Zhan-mei Wang Shu-Ping Pan Jing-Jing Zhang jun zhou Department of Medical Oncology Qilu Hospital(Qingdao)Cheeloo College MedicineShandong UniversityQingdao 266000Shandong ProvinceChina Department of Gastroenterology Feicheng People’s HospitalFeicheng 271600Shandong ProvinceChina Department of Anus and Intestine Surgery Xiangya Hospital of Central South UniversityChangsha 410008Hunan ProvinceChina Department of Oncology Qilu Hospital(Qingdao)Cheeloo College MedicineShandong UniversityQingdao 266000Shandong ProvinceChina

BACKGROUND Colorectal cancer(CRC)is a common malignant tumor,and liver metastasis is one of the main recurrence and metastasis modes that seriously affect patients’survival rate and quality of *** such as albumin bilirubin(ALBI)score,liver function index,and carcinoembryonic antigen(CEA)have shown some potential in the prediction of liver metastasis but have not been fully *** To evaluate its predictive value for liver metastasis of CRC by conducting the combined analysis of ALBI,liver function index,and CEA,and to provide a more accurate liver metastasis risk assessment tool for clinical treatment *** This study retrospectively analyzed the clinical data of patients with CRC who received surgical treatment in our hospital from January 2018 to July 2023 and were followed up for 24 *** to the follow-up results,the enrolled patients were divided into a liver metastasis group and a nonliver metastasis group and randomly divided into a modeling group and a verification group at a ratio of 2:*** risk factors for liver metastasis in patients with CRC were analyzed,a prediction model was constructed by least absolute shrinkage and selection operator(LASSO)logistic regression,internal validation was performed by the bootstrap method,the reliability of the prediction model was evaluated by subject-work characteristic curves,calibration curves,and clinical decision curves,and a column graph was drawn to show the prediction *** Of 130 patients were enrolled in the modeling group and 65 patients were enrolled in the verification group out of the 195 patients with CRC who fulfilled the inclusion and exclusion *** LASSO regression variable screening and logistic regression *** ALBI score,alanine aminotransferase(ALT),and CEA were found to be independent predictors of liver metastases in CRC patients[odds ratio(OR)=8.062,95%confidence interval(CI):2.545-25.540],(OR=1.037,95%CI:1.004-1.071)and(OR=1.0

关键词： Albumin-bilirubin Carcinoembryonic antigen Colorectal cancer Tumor metastasis Prediction model