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Journal of Materials Science & Technology 2020年第20期52卷 152-158页

作者： Na Li Fei chen Xiangtao chen zhongxu chen Yang Qi Xiaodong Li Xudong Sun Key Laboratory for Anisotropy and Texture of Materials(Ministry of Education) School of Materials Science and EngineeringNortheastern UniversityShenyang 110819China College of Environmental and Chemical Engineering Dalian UniversityDalian 116622China

Lithium-sulfur batteries hold promise for next generation batteries due to their high theoretical energy density and low ***,the rapid capacity fading caused by the shuttle of polysulfide between two electrodes severely hinders the practical application of Li-S *** address the issue,we reported a three-dimensional heterostructured TiO2 nanosheets/N-doped carbon(TO/NC),which is coated on a commercial polypro pylene(PP) separator,as an efficient barrier for Li-S *** TO/NC coating layer provides a bipolar chemical adsorption of lithium polysulfides(LiPSs) via TiO2 nanosheets with exposed(001) facets and N-doped carbon,showing high trapping capacity and remarkable electrocatalytic activity for *** slurry-bladed carbon black/sulfur cathode with 64 wt% sulfur offers outstanding performance with an initial capacity of 1314 mAh g^-1 at 0.2 *** 900 cycles,the cell still maintains the capacity of 448 mA h g^-1 at a 1 C rate with a degradation rate of only 0.055% per *** separator reported in this work holds great promise for the development of high-energy Li-S batteries.

关键词： Lithium-sulfur batteries N-doped carbon Separator TiO2 Nanosheets

WheatGmap:a comprehensive platform for wheat gene mapping and genomic studies

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Molecular Plant 2021年第2期14卷 187-190页

作者： Lichao Zhang Chunhao Dong zhongxu chen Lixuan Gui cheng chen Danping Li Zhencheng Xie Qiang Zhang Xueying Zhang Chuan Xia Xu Liu Xiuying Kong Jirui Wang Key Laboratory for Crop Gene Resources and Germplasm Enhancement Ministry of Agriculture and Rural Affairs/National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Triticeae Research Institute Sichuan Agricultural UniversityChengdu 611130China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130China Department of Life Science TcuniInc.Chengdu 610041China

Bulked segregant analysis(BSA)is an efficient and low-cost strategy that is widely used to identify causal genes in segregating ***-based methods,such as BSA sequencing(Wenger et al.,2010),bulked segregant RNA sequencing(BSR-seq)(del Viso et al.,2012),and MutMap(Abe et al.,2012),are powerful tools that can be used for rapidly discovering genetic markers and gene *** BSA is increasingly being used in wheat(Triticum aestivum)gene mapping efforts,few user-friendly BSA tools have been developed for researchers lacking a strong bioinformatics ***,we developed the web-based BSA platform WheatGmap(https://***),which integrates multiple BSA mapping models and large amounts of public data to accelerate gene cloning and functional research and facilitate resource sharing.

关键词： aestivum Wheat comprehensive

Genome sequencing of Sitopsis species provides insights into their contribution to the B subgenome of bread wheat

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Plant Communications 2023年第4期4卷 208-224页

作者： Yuxin Yang Licao Cui Zefu Lu Guangrong Li Zujun Yang Guangyao Zhao Chuizheng Kong Danping Li Yaoyu chen Zhencheng Xie zhongxu chen Lichao Zhang Chuan Xia Xu Liu Jizeng Jia Xiuying Kong State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Center for Informational Biology School of Life Science and TechnologyUniversity of Electronic Science and Technology of ChinaChengdu 610054China Triticeae Research Institute Sichuan Agricultural UniversityChengdu 611130China

Wheat(Triticum aestivum,BBAADD)is an allohexaploid species that originated from two polyploidization *** progenitors of the A and D subgenomes have been identified as Triticum urartu and Aegilops tauschii,*** research suggests that Aegilops speltoides is the closest but not the direct ancestor of the B ***,whether *** has contributed genomically to the wheat B subgenome and which chromosome regions are conserved between *** and the B subgenome remain ***,we assembled a high-quality reference genome for ***,resequenced 53 accessions from seven species(Aegilops bicornis,Aegilops longissima,Aegilops searsii,Aegilops sharonensis,***,Aegilops mutica[*** muticum],and Triticumdicoccoides)and revealed their genomic contributions to the wheat B *** results showed that centromeric regions were particularly conserved between Aegilops and Triticum and revealed 0.17 Gb of conserved blocks between *** and the B *** classified five groups of conserved and non-conserved genes between Aegilops and Triticum,revealing their biological characteristics,differentiation in gene expression patterns,and collinear relationships between *** and the wheat B *** also identified gene families that expanded in *** during its evolution and 789 genes specific to *** genes can serve as genetic resources for improvement of adaptability to biotic and abiotic *** newly constructed reference genome and large-scale resequencing data for Sitopsis species will provide a valuable genomic resource for wheat genetic improvement and genomic studies.

关键词： Aegilops Sitopsis polyploid wheat B subgenome conserved blocks

Rice Bran-derived Peptide KF-8 Attenuates Dexamethasone-induced Myopathy in Caenorhabditis elegans by Regulating Locomotion-related Genes

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Food Science and Human Wellness 2024年

作者： Yixin Wu Jianqiang Wang Fang Huang Yajuan chen Qinlu Lin zhongxu chen Zhigang Liu Yao Jiang Wenqing Xie Hengzhen Li Yusheng Li Ying Liang Molecular Nutrition Branch， National Engineering Research Center of Rice and By-product Deep Processing， College of Food Science and Engineering， Central South University of Forestry and Technology Laboratory of Functional Chemistry and Nutrition of Food， College of Food Science and Engineering， Northwest A&F University The Key Lab of Non-wood Forest Cultivation and Protection of Education Ministry， The Key Lab of Non-wood Forest Products of Forestry Ministry， Forestry Institute， Central South University of Forestry & Technology Department of Orthopedics， Xiangya Hospital， Central South University National Clinical Research Center for Geriatric Disorders， Xiangya Hospital， Central South University

Background and aims: Dexamethasone is a common glucocorticoid medication with adverse effects that can cause muscle atrophy， but no drug intervention has been approved or recommended for this condition. KF-8 is a rice bran-derived anti-oxidant peptide that extends the life span of Caenorhabditis elegans. Methods: We established a C. elegans model of dexamethasone-induced myopathy to evaluate the potential therapeutic effects of KF-8 in this model. C. elegans muscle function was assessed in terms of locomotory behaviors including crawling， swimming， burrowing， pharyngeal pumping， and head swing. Muscle actin filament integrity was evaluated using fluorescence imaging. The molecular mechanisms of KF-8 were investigated using transcriptome sequencing， qRT-PCR， RNA interference， and western blot analysis. Results: Dexamethasone disrupted actin filaments in the striated muscles of the body wall and inhibited C. elegans crawling， swimming， burrowing， pharyngeal pumping， and head swing. KF-8 reversed the actin filament disruption and locomotor dysfunction induced by dexamethasone. Transcriptome sequencing， pathway enrichment， and qRT-PCR analyses revealed that KF-8 regulated the locomotion-related genes W04G5.10， vha-12， and ddr-1， as well as age-1 (the catalytic subunit ortholog of PI3K)， and akt1. RNA interference， conducted using a genetically engineered E. coli HT115 strain as a food source， confirmed age-1 as a key regulator of locomotor function of C. elegans. Further mechanistic studies with C2C12 myotubes showed that KF-8 regulated the IRS-PI3K-Akt pathway， the master regulator of protein synthesis and degradation. Conclusion: Together， these findings suggest that KF-8 protects against dexamethasone-induced myopathy in C. elegans by regulating locomotion-related genes and the IRS-PI3K-Akt pathway.

关键词：

Systematic identification of wheat spike developmental regulators by integrated multiomics, transcriptional network, GWAS, and genetic analyses

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Molecular Plant 2024年第3期17卷 438-459页

作者： Xuelei Lin Yongxin Xu Dongzhi Wang Yiman Yang Xiaoyu Zhang Xiaomin Bie Lixuan Gui zhongxu chen Yiliang Ding Long Mao Xueyong Zhang Fei Lu Xiansheng Zhang Cristobal Uauyo Xiangdong Fu Jun Xiao Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Nanjing Agricultural University NanjingJiangsu 210095China Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityTaianShandong 271018China Department of Life Science Tcuni Inc.ChengduSichuan 610000China John Innes Centre Norwich Research ParkNorwich NR47UHUK Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China CAS-JIC Centre of Excellence for Plant and Microbial Science(CEPAMS) Institute of Genetics and Developmental BiologyCASBeijing 100101China

The spike architecture of wheat plays a crucial role in determining grain number,making it a key trait for optimization in wheat breeding *** this study,we used a multi-omic approach to analyze the transcriptome and epigenome profiles of the young spike at eight developmental stages,revealing co-ordinated changes in chromatin accessibility and H3K27me3 abundance during the flowering *** constructed a core transcriptional regulatory network(TRN)that drives wheat spike formation and experimentally validated a multi-layer regulatorymodule involving TaSPL15,TaAGLG1,and *** integrating the TRN with genome-wide association studies,we identified 227 transcription factors,including 42 with known functions and 185 with unknown *** investigation of 61 novel transcription factors using multiple homozygous mutant lines revealed 36 transcription factors that regulate spike architecture or flowering time,such as TaMYC2-A1,TaMYB30-A1,and *** particular interest,TaMYB30-A1,downstream of and repressed by WFzP,was found to regulate fertile spikelet ***,the excellent haplotype of TaMYB30-A1,which contains a C allele at the WFzP binding site,was enriched during wheat breeding improvement in China,leading to improved agronomic ***,we constructed a free and open access Wheat Spike Multi-Omic Database(http://39.***.48.156:8800/#/).Our study identifies novel and high-confidence regulators and offers an effective strategy for dissecting the genetic basis of wheat spike development,with practical value forwheat breeding.

关键词： wheat spike development epigenome TRN TaMYB30-A1 breeding selection

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植源肽对啮齿动物神经退行性疾病的影响

中国食品学报 2021年第7期21卷 360-370页

作者：蔡洁崔校基唐柳欢陈忠旭梁盈中南林业科技大学稻谷及副产物深加工国家工程实验室长沙410004

神经退行性疾病是世界主要公共卫生问题之一,然而,目前缺乏治疗神经退行性疾病的有效药物,膳食营养干预是目前最受欢迎的干预手段之一。植源肽通过诸多途径在人类健康中发挥重要作用,如抗氧化、抗炎、保护神经系统等。本文综述植源肽对... 详细信息

神经退行性疾病是世界主要公共卫生问题之一,然而,目前缺乏治疗神经退行性疾病的有效药物,膳食营养干预是目前最受欢迎的干预手段之一。植源肽通过诸多途径在人类健康中发挥重要作用,如抗氧化、抗炎、保护神经系统等。本文综述植源肽对啮齿动物神经退行性疾病的作用机制,并展望其在神经退行性疾病应用中的机遇与挑战。

关键词：植源肽退行性疾病啮齿动物

Boosting wheat functional genomics via an indexed EMS mutant library of KN9204

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Plant Communications 2023年第4期4卷 58-76页

作者： Dongzhi Wang Yongpeng Li Haojie Wang Yongxin Xu Yiman Yang Yuxin Zhou zhongxu chen Yuqing Zhou Lixuan Gui Yi Guo Chunjiang Zhou Wenqiang Tang Shuzhi Zheng Lei Wang Xiulin Guo Yingjun Zhang Fa Cui Xuelei Lin Yuling Jiao Yuehui He Junming Li Fei He Xigang Liu Jun Xiao Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Ministry of Education Key Laboratory of Molecular and Cellular Biology Hebei Key Laboratory of Molecular and Cellular BiologyCollege of Life SciencesHebei Normal UniversityHebei Collaboration Innovation Center for Cell SignalingShijiazhuang 050024China Center for Agricultural Resources Research Institute of Genetics and Development BiologyChinese Academy of SciencesShijiazhuang 050022China University of Chinese Academy of Sciences Beijing 100049China Department of Life Science TcuniIncChengdu 610000China State Key Laboratory of Protein and Plant Gene Research Peking-Tsinghua Center for Life SciencesSchool of Life SciencesCenter for Quantitative BiologyPeking UniversityBeijing 100871China State Key Laboratory of Protein and Plant Gene Research School of Advanced Agricultural SciencesPeking-Tsinghua Center for Life SciencesPeking UniversityBeijing 100871China Plant Genetic Engineering Center of Hebei Province Institute of Biotechnology and Food ScienceHebei Academy of Agriculture and Forestry SciencesShijiazhuang 050051China Institute ofCereal and OilCrops Hebei Academy of Agriculture and Forestry SciencesLaboratory of CropGenetics andBreeding ofHebeiShijiazhuang 050035China Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in Universities of Shandong College of AgricultureLudong UniversityYantai 264025China Centre of Excellence for Plant and Microbial Science(CEPAMS) JIC-CASBeijing 100101China

A better understanding of wheat functional genomics can improve targeted breeding for better agronomic traits and environmental ***,the lack of gene-indexed mutants and the low transformation efficiency of wheat limit in-depth gene functional studies and genetic manipulation for *** this study,we created a library for KN9204,a popular wheat variety in northern China,with a reference genome,transcriptome,and epigenome of different tissues,using ethyl methyl sulfonate(EMS)*** library contains a vast developmental diversity of critical tissues and transition *** capture sequencing of 2090 mutant lines using KN9204 genome-designed probes revealed that 98.79%of coding genes had mutations,and each line had an average of 1383 EMS-type *** identified new allelic variations for crucial agronomic trait-related genes such as Rht-D1,Q,TaTB1,and *** tested 100 lines with severemutations in 80 NAC transcription factors(TFs)under drought and salinity stress and identified 13 lines with altered *** analysis of three lines using transcriptome and chromatin accessibility data revealed hundreds of direct NAC targets with altered transcription patterns under salt or drought stress,including SNAC1,DREB2B,CML16,and ZFP182,factors known to respond to abiotic ***,we have generated and indexed a KN9204 EMS mutant library that can facilitate functional genomics research and offer resources for genetic manipulation of wheat.

关键词： wheat exome capture sequencing EMS mutagenesis functional genomics

密云水库库滨带不同植被配置下面源污染特征分析

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生态环境学报 2019年第6期28卷 1183-1192页

作者：李凯程金花陈仲旭北京林业大学水土保持学院

密云水库是北京市重要地表水源地,在首都经济发展中发挥着重要作用。随着密云水库水位上涨以及库滨带工程建设,其生态环境发生了一系列变化。通过设立12个不同植被配置的野外标准径流小区,研究天然降雨条件下不同植被配置对于总氮(TN)... 详细信息

密云水库是北京市重要地表水源地,在首都经济发展中发挥着重要作用。随着密云水库水位上涨以及库滨带工程建设,其生态环境发生了一系列变化。通过设立12个不同植被配置的野外标准径流小区,研究天然降雨条件下不同植被配置对于总氮(TN)、总磷(TP)、氨氮(NH4^+-N)、化学需氧量(COD)、生物需氧量(BOD5)5个面源污染指标的影响,以期为库滨带建设提供参考数据。研究结果表明,(1)天然降雨条件下乔灌草混交小区柳树(Salix matsudana)+丁香(Syringa oblata)+蒿(Artemisia annua)的减流作用最好,平均减流作用为62.9%,减沙作用最好的是毛白杨(Populus tomentosa)+紫丁香(Syringa oblata)+葎草(Humulus scandens)小区,减沙作用为78.18%。(2)植被对于面源污染有着显著的控制效果,毛白杨+紫丁香+葎草的乔灌草配置控制效果最好,对TN、TP、NH4^+-N、COD、BOD5的削减率在77.76%-92.24%之间。(3)3次降雨条件下各植被配置小区随着时间的推移、降雨量的减少、降雨强度的减弱,TN的流失模数显著减少(P<0.05);TP流失变化较大,但总体上呈减小的趋势;同一种植被配置下NH4+-N流失模数显著减少(P<0.05);COD和BOD5下降趋势明显。在各小区随时间及降雨条件变化的面源污染控制削减纵向比较中,3次天然降雨条件下,5个面源污染指标呈递减趋势。对于TP的削减控制,油松(Pinus tabuliformis)+臭椿(Ailanthus altissima)小区最优;而其余4个指标,削减比最高的均为油松+金叶榆(Ulmus pumila)小区。由此可见此种植被配置模式对于面源污染控制的长期效益最好。

关键词：径流植被配置面源污染

基于反向聚类法的公铁分离桥吊装施工安全风险因素评价

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铁道建筑技术 2024年第6期 118-121页

作者：陈忠旭中铁十九局集团第一工程有限公司辽宁辽阳111000

本文以公铁分离桥吊装施工为例,基于反向聚类法分别从五个方面选取二十六项典型风险因素,对公铁分离桥吊装施工安全风险因素进行评价。结果表明,秩效应最大风险因素为违章操作,其值为41.56,是影响桥梁吊装施工安全风险主要因素;对桥梁... 详细信息

本文以公铁分离桥吊装施工为例,基于反向聚类法分别从五个方面选取二十六项典型风险因素,对公铁分离桥吊装施工安全风险因素进行评价。结果表明,秩效应最大风险因素为违章操作,其值为41.56,是影响桥梁吊装施工安全风险主要因素;对桥梁吊装施工影响程度最低的是吊装安全警示标识不明显,其值为11.11。对桥梁吊装施工安全风险影响程度较高因素为第一类与第二类分析,人员、现场管理、技术方案等为影响梁桥吊装施工关键因素;低于平均水平的风险因素为第四类、第五类,主要包括环境、设备等因素。结合分析,提出桥梁吊装施工安全管理措施,包括吊机支腿作业安全管理、装吊工安全管理、施工现场联系安全管理、现场防护人员安全管理。

关键词：反向聚类法吊装施工安全风险评价

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浅谈混凝土外加剂在西藏的发展及应用

西藏科技 2009年第4期 58-60页

作者：陈忠旭西藏天路有限公司西藏拉萨850000

混凝土外加剂能有效地改善混凝土的性能、提高工程质量、提高技术经济效益,因而受到国内外的普遍重视。本文简要介绍了混凝土外加剂在西藏发展及应用。

关键词：混凝土外加剂西藏发展应用