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Genome-wide identification of recombination rates of male versus female gametes in interspecific populations of cotton

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Genome-wide identification of recombination rates of male ve...

新疆彩色棉23个品种指纹图谱的构建及遗传多样性分析

第十三届全国植物基因组学大会

作者： Yu Yu~(1,2),Xianlong Zhang~1,zhongxu lin~(1*) 1 State Key Laboratory of crop genetic improvement,Huazhong Agricultural University, Wuhan 430070,China 2 Cotton Research Institute,Academy of Sciences of Xinjiang farming cultivate,Xinjiang Shihezi 832000,China

Based on the established SSR-based BC1 genetic linkage map,2 genetic maps covered with 313 markers have been developed for 2 more backcross populations which was aimed to study the difference of recombination rates be...

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棉花学报 2014年第2期26卷 161-170页

作者：尤春源聂新辉张胜郭欢乐王夏青林忠旭石河子农业科技开发研究中心棉花研究所新疆石河子832000 华中农业大学作物遗传改良国家重点实验室湖北武汉430070 新疆自治区种子管理总站新疆乌鲁木齐832006

以新疆截止2012年审定的23份新彩棉品种为材料,利用SSR标记进行DNA指纹图谱的构建和遗传多样性分析.从5000对SSR引物中,挑选出多态性高、稳定性好、均匀分布在棉花26条染色体上的52对引物,在23份新彩棉品种中筛选出核心引物47对,SSR扩... 详细信息

以新疆截止2012年审定的23份新彩棉品种为材料,利用SSR标记进行DNA指纹图谱的构建和遗传多样性分析.从5000对SSR引物中,挑选出多态性高、稳定性好、均匀分布在棉花26条染色体上的52对引物,在23份新彩棉品种中筛选出核心引物47对,SSR扩增检测到多态性基因型位点数共计162个,每个标记检测到的基因型位点数在2～7之间,平均为3.45个;引物多态信息量（PIC）值介于0.4537～0.8686之间,平均值为0.7096.结果显示：在23份新彩棉品种中,14份品种采用特异或特征引物可以一次性区分开,其余9份品种需要采用引物组合来实现区别该品种与其他品种.最少选用18对特异引物及组合引物就可以完全区分开新彩棉1～23号品种.利用18对SSR标记构建了新彩棉1号至23号品种的指纹图谱.利用NTSYS-pcV2.10软件聚类分析表明：23个新彩棉品种遗传相似系数变化范围是0.3781～0.9298,平均为0.5511,表明新彩棉品种之间存在着丰富的遗传多样性.

关键词：彩色棉 DNA指纹图谱遗传多样性

High-throughput research on superconductivity

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Chinese Physics B 2018年第12期27卷 9-15页

作者： Mingyang Qin Zefeng lin zhongxu Wei Beiyi Zhu Jie Yuan Kui Jin Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190China Songshan Lake Materials Laboratory Dongguan 523808China Department of Materials Science and Engineering and Center for Nanophysics and Advanced MaterialsUniversity of MarylandCollege ParkMaryland 20742USA

As an essential component of the Materials Genome Initiative aiming to shorten the period of materials research and development, combinatorial synthesis and rapid characterization technologies have been playing a more and more important role in exploring new materials and comprehensively understanding materials properties. In this review, we discuss the advantages of high-throughput experimental techniques in researches on superconductors. The evolution of combinatorial thin-film technology and several high-speed screening devices are briefly introduced. We emphasize the necessity to develop new high-throughput research modes such as a combination of high-throughput techniques and conventional methods.

关键词： superconductivity materials genome initiative high-throughput experimental technology high-throughput research mode

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基于黄褐棉导入系群体定位抗黄萎病QTL

棉花学报 2023年第1期35卷 29-38页

作者：常鑫燚李轩照唐秉晖潘振远吴元龙沈超努日曼古丽·艾尼林忠旭尤春源聂新辉石河子大学农学院/新疆生产建设兵团绿洲生态农业重点实验室新疆石河子832003 石河子农业科学院棉花研究所新疆石河子832011 广东石油化工学院生物与食品工程学院广东茂名525000 华中农业大学植物科学技术学院/作物遗传改良国家重点实验室武汉430070

【目的】黄萎病(Verticillium wilt)是棉花生产中的最主要病害,且棉花黄萎病的致病机理尚不清楚。通过构建黄褐棉导入系群体定位棉花黄萎病抗性相关的数量性状位点(quantitative trait loci,QTL),为抗黄萎病分子标记开发和辅助育种提供... 详细信息

【目的】黄萎病(Verticillium wilt)是棉花生产中的最主要病害,且棉花黄萎病的致病机理尚不清楚。通过构建黄褐棉导入系群体定位棉花黄萎病抗性相关的数量性状位点(quantitative trait loci,QTL),为抗黄萎病分子标记开发和辅助育种提供参考。【方法】以陆地棉(Gossypium hirsutum)B0011为轮回亲本、黄褐棉(***)为供体亲本,构建有71个株系的BC_(5)S_(5)群体。利用2 839个单核苷酸多态性(single nucleotide polymorphism,SNP)标记结合表型值进行黄萎病抗性相关QTL定位。【结果】共检测到15个与黄萎病抗性相关的QTL,可解释4.21%~26.77%的表型变异。加性效应分析表明:其中6个QTL的有利等位基因来源于黄褐棉,9个QTL有利等位基因来自B0011。同时,qVW-A01-1、q VW-A02-2和qVW-A07-2在2个及以上环境中被检测到,表型变异解释率分别为15.56%~16.56%、11.95%~24.62%和13.22%~16.73%。利用BC5S5群体黄萎病抗性的最佳线性无偏预测值(best linear unbiased prediction,BLUP)进行QTL定位,共检测到5个QTL,其中qVW-A01-1B、q VW-A02-1B分别与加性效应分析稳定检测到的qVW-A01-1、qVW-A02-2物理位置一致,分别解释23.67%和17.90%的表型变异。【结论】本研究发现2个稳定的QTL即qVW-A01-1和qVW-A02-2,可为抗黄萎病分子标记辅助选择育种及候选基因功能鉴定奠定基础。

关键词：陆地棉抗病育种黄萎病数量性状位点分子标记辅助育种

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新疆陆地棉经济性状优异等位基因位点的遗传解析

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棉花学报 2020年第3期32卷 185-198页

作者：雷杰杰邵盘霞郭春平张大伟唐秉晖努日曼古丽·艾尼彭亚娟崔天宇张奥深林海荣林忠旭尤春源聂新辉石河子大学农学院/绿洲生态重点实验室新疆石河子832003 石河子农业科学研究院棉花所新疆石河子832000 华中农业大学植物科学技术学院/作物遗传改良国家重点实验室武汉430070 新疆农业科学院经济作物研究所乌鲁木齐830091

【目的】基于SSR(Simple sequence repeat,简单序列)标记展开对陆地棉经济性状的关联分析,挖掘优异等位变异位点,解析新疆陆地棉经济性状的遗传基础,为新疆陆地棉的遗传机理研究和高效的陆地棉分子设计育种提供理论依据。【方法】利用... 详细信息

【目的】基于SSR(Simple sequence repeat,简单序列)标记展开对陆地棉经济性状的关联分析,挖掘优异等位变异位点,解析新疆陆地棉经济性状的遗传基础,为新疆陆地棉的遗传机理研究和高效的陆地棉分子设计育种提供理论依据。【方法】利用筛选出覆盖棉花全基因组的73对SSR标记对新疆156份陆地棉品种进行多态性扫描;采用R语言绘制boxplot图,利用TASSEL软件进行关联分析,挖掘与产量、纤维品质性状相关联的优异等位变异位点。【结果】通过对6个环境下新疆陆地棉品种的产量、品质相关性状进行关联分析,获得与产量性状相关的等位变异位点10个,表型变异解释率为6.69%~9.88%,平均值为8.43%;与纤维品质性状相关的等位变异位点23个,表型变异解释率为3.73%~13.22%,平均值为7.52%。其中22个QTL(Quantitative trait locus)已被报道,有10个QTL的关联性状与前人研究一致。【结论】新疆陆地棉品种的群体遗传结构简单,连锁不平衡水平低,6个环境条件下表型性状变化趋势较稳定。基于SSR的关联分析,发掘了与产量和纤维品质相关的优异等位变异基因及聚合优异等位基因位点的典型材料。

关键词：陆地棉新疆产量性状纤维品质关联分析

Systematic identification of wheat spike developmental regulators by integrated multiomics, transcriptional network, GWAS, and genetic analyses

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Molecular Plant 2024年第3期17卷 438-459页

作者： Xuelei lin Yongxin Xu Dongzhi Wang Yiman Yang Xiaoyu Zhang Xiaomin Bie Lixuan Gui zhongxu Chen Yiliang Ding Long Mao Xueyong Zhang Fei Lu Xiansheng Zhang Cristobal Uauyo Xiangdong Fu Jun Xiao Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Nanjing Agricultural University NanjingJiangsu 210095China Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityTaianShandong 271018China Department of Life Science Tcuni Inc.ChengduSichuan 610000China John Innes Centre Norwich Research ParkNorwich NR47UHUK Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China CAS-JIC Centre of Excellence for Plant and Microbial Science(CEPAMS) Institute of Genetics and Developmental BiologyCASBeijing 100101China

The spike architecture of wheat plays a crucial role in determining grain number,making it a key trait for optimization in wheat breeding *** this study,we used a multi-omic approach to analyze the transcriptome and epigenome profiles of the young spike at eight developmental stages,revealing co-ordinated changes in chromatin accessibility and H3K27me3 abundance during the flowering *** constructed a core transcriptional regulatory network(TRN)that drives wheat spike formation and experimentally validated a multi-layer regulatorymodule involving TaSPL15,TaAGLG1,and *** integrating the TRN with genome-wide association studies,we identified 227 transcription factors,including 42 with known functions and 185 with unknown *** investigation of 61 novel transcription factors using multiple homozygous mutant lines revealed 36 transcription factors that regulate spike architecture or flowering time,such as TaMYC2-A1,TaMYB30-A1,and *** particular interest,TaMYB30-A1,downstream of and repressed by WFzP,was found to regulate fertile spikelet ***,the excellent haplotype of TaMYB30-A1,which contains a C allele at the WFzP binding site,was enriched during wheat breeding improvement in China,leading to improved agronomic ***,we constructed a free and open access Wheat Spike Multi-Omic Database(http://39.***.48.156:8800/#/).Our study identifies novel and high-confidence regulators and offers an effective strategy for dissecting the genetic basis of wheat spike development,with practical value forwheat breeding.

关键词： wheat spike development epigenome TRN TaMYB30-A1 breeding selection

Rice Bran-derived Peptide KF-8 Attenuates Dexamethasone-induced Myopathy in Caenorhabditis elegans by Regulating Locomotion-related Genes

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Food Science and Human Wellness 2024年

作者： Yixin Wu Jianqiang Wang Fang Huang Yajuan Chen Qinlu lin zhongxu Chen Zhigang Liu Yao Jiang Wenqing Xie Hengzhen Li Yusheng Li Ying Liang Molecular Nutrition Branch， National Engineering Research Center of Rice and By-product Deep Processing， College of Food Science and Engineering， Central South University of Forestry and Technology Laboratory of Functional Chemistry and Nutrition of Food， College of Food Science and Engineering， Northwest A&F University The Key Lab of Non-wood Forest Cultivation and Protection of Education Ministry， The Key Lab of Non-wood Forest Products of Forestry Ministry， Forestry Institute， Central South University of Forestry & Technology Department of Orthopedics， Xiangya Hospital， Central South University National Clinical Research Center for Geriatric Disorders， Xiangya Hospital， Central South University

Background and aims: Dexamethasone is a common glucocorticoid medication with adverse effects that can cause muscle atrophy， but no drug intervention has been approved or recommended for this condition. KF-8 is a rice bran-derived anti-oxidant peptide that extends the life span of Caenorhabditis elegans. Methods: We established a C. elegans model of dexamethasone-induced myopathy to evaluate the potential therapeutic effects of KF-8 in this model. C. elegans muscle function was assessed in terms of locomotory behaviors including crawling， swimming， burrowing， pharyngeal pumping， and head swing. Muscle actin filament integrity was evaluated using fluorescence imaging. The molecular mechanisms of KF-8 were investigated using transcriptome sequencing， qRT-PCR， RNA interference， and western blot analysis. Results: Dexamethasone disrupted actin filaments in the striated muscles of the body wall and inhibited C. elegans crawling， swimming， burrowing， pharyngeal pumping， and head swing. KF-8 reversed the actin filament disruption and locomotor dysfunction induced by dexamethasone. Transcriptome sequencing， pathway enrichment， and qRT-PCR analyses revealed that KF-8 regulated the locomotion-related genes W04G5.10， vha-12， and ddr-1， as well as age-1 (the catalytic subunit ortholog of PI3K)， and akt1. RNA interference， conducted using a genetically engineered E. coli HT115 strain as a food source， confirmed age-1 as a key regulator of locomotor function of C. elegans. Further mechanistic studies with C2C12 myotubes showed that KF-8 regulated the IRS-PI3K-Akt pathway， the master regulator of protein synthesis and degradation. Conclusion: Together， these findings suggest that KF-8 protects against dexamethasone-induced myopathy in C. elegans by regulating locomotion-related genes and the IRS-PI3K-Akt pathway.

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Boosting wheat functional genomics via an indexed EMS mutant library of KN9204

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Plant Communications 2023年第4期4卷 58-76页

作者： Dongzhi Wang Yongpeng Li Haojie Wang Yongxin Xu Yiman Yang Yuxin Zhou zhongxu Chen Yuqing Zhou Lixuan Gui Yi Guo Chunjiang Zhou Wenqiang Tang Shuzhi Zheng Lei Wang Xiulin Guo Yingjun Zhang Fa Cui Xuelei lin Yuling Jiao Yuehui He Junming Li Fei He Xigang Liu Jun Xiao Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Ministry of Education Key Laboratory of Molecular and Cellular Biology Hebei Key Laboratory of Molecular and Cellular BiologyCollege of Life SciencesHebei Normal UniversityHebei Collaboration Innovation Center for Cell SignalingShijiazhuang 050024China Center for Agricultural Resources Research Institute of Genetics and Development BiologyChinese Academy of SciencesShijiazhuang 050022China University of Chinese Academy of Sciences Beijing 100049China Department of Life Science TcuniIncChengdu 610000China State Key Laboratory of Protein and Plant Gene Research Peking-Tsinghua Center for Life SciencesSchool of Life SciencesCenter for Quantitative BiologyPeking UniversityBeijing 100871China State Key Laboratory of Protein and Plant Gene Research School of Advanced Agricultural SciencesPeking-Tsinghua Center for Life SciencesPeking UniversityBeijing 100871China Plant Genetic Engineering Center of Hebei Province Institute of Biotechnology and Food ScienceHebei Academy of Agriculture and Forestry SciencesShijiazhuang 050051China Institute ofCereal and OilCrops Hebei Academy of Agriculture and Forestry SciencesLaboratory of CropGenetics andBreeding ofHebeiShijiazhuang 050035China Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in Universities of Shandong College of AgricultureLudong UniversityYantai 264025China Centre of Excellence for Plant and Microbial Science(CEPAMS) JIC-CASBeijing 100101China

A better understanding of wheat functional genomics can improve targeted breeding for better agronomic traits and environmental ***,the lack of gene-indexed mutants and the low transformation efficiency of wheat limit in-depth gene functional studies and genetic manipulation for *** this study,we created a library for KN9204,a popular wheat variety in northern China,with a reference genome,transcriptome,and epigenome of different tissues,using ethyl methyl sulfonate(EMS)*** library contains a vast developmental diversity of critical tissues and transition *** capture sequencing of 2090 mutant lines using KN9204 genome-designed probes revealed that 98.79%of coding genes had mutations,and each line had an average of 1383 EMS-type *** identified new allelic variations for crucial agronomic trait-related genes such as Rht-D1,Q,TaTB1,and *** tested 100 lines with severemutations in 80 NAC transcription factors(TFs)under drought and salinity stress and identified 13 lines with altered *** analysis of three lines using transcriptome and chromatin accessibility data revealed hundreds of direct NAC targets with altered transcription patterns under salt or drought stress,including SNAC1,DREB2B,CML16,and ZFP182,factors known to respond to abiotic ***,we have generated and indexed a KN9204 EMS mutant library that can facilitate functional genomics research and offer resources for genetic manipulation of wheat.

关键词： wheat exome capture sequencing EMS mutagenesis functional genomics

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传马麟《三官出巡图》之创作年代与宗教意涵

中国国家博物馆馆刊 2024年第10期 80-109页

作者：王中旭故宫博物院北京100009

传马麟《三官出巡图》是台北故宫博物院藏道教画作,但是关于该画的年代,一直有南宋、元、明等不同说法。从该图中方心曲领、立笔、辂的形制,及神灵衣纹线条、山石皴法等来看,该图应系明前期宫廷或民间职业画家所作。包括该图在内的宋代... 详细信息

传马麟《三官出巡图》是台北故宫博物院藏道教画作,但是关于该画的年代,一直有南宋、元、明等不同说法。从该图中方心曲领、立笔、辂的形制,及神灵衣纹线条、山石皴法等来看,该图应系明前期宫廷或民间职业画家所作。包括该图在内的宋代以来的三官出行图,表现的应是三元日三官降临人间的场景。该图中对功过簿、麒麟的表现,及土地神,龙王、龙女的加入,反映了明代以来三官图像及信仰的祥瑞化、世俗化。

关键词：三官图名物风格降临祥瑞化