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An RnA-RnA crosstalk network involving HMGB1 and RICTOR facilitates hepatocellular carcinoma tumorigenesis by promoting glutamine metabolism and impedes immunotherapy by PD-L1+ exosomes activity

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signal Transduction and Targeted Therapy 2022年第1期7卷 282-294页

作者： Yanping Wei Xuewu Tang Yibin Ren Yun Yang Fengliang song Jingbo Fu shuowu Liu Miao Yu Jing Chen suyang Wang Kecheng Zhang Yexiong Tan Zhipeng Han Lixjn Wei baohua Zhang Zhangjun Cheng Liang Li Hongyang Wang International Co-operation Laboratory on signal Transduction Eastern Hepato-Biliary Surgery InstituteSecond Military Medical UniversityShanghaiChina national Center for Liver Cancer ShanghaiChina Hepato-Pancreato-biliary center Zhongda HospitalSchool of MedicineSoutheast UniversityNanjingChina The Third Department of Hepatic surgery Eastern Hepatobiliary Surgery HospitalShanghaiChina school of Medicine Nantong UniversityNantongJiangsu ProvinceChina Department of Biliary Tract surgery Eastern Hepatobiliary Surgery HospitalSecond Military Medical UniversityShanghaiChina Tumor Immunology and Gene Therapy Center Eastern Hepato-Biliary Surgery InstituteSecond Military Medical UniversityShanghaiChina national Laboratory for Oncogenes and Related Genes Cancer InstituteRenJi HospitalShanghai Jiao Tong University200441 ShanghaiChina

Hepatocellular carcinoma(HCC)is the global leading cause of cancer-related deaths due to the deficiency of targets for precision therapy.A new modality of epigenetic regulation has emerged involving RnA-RnA crosstalk networks where two or more competing endogenous RnAs(ceRnAs)bind to the same ***,the contribution of such mechanisms in HCC has not been well ***,potential HMGB1-driven RnA-RnA crosstalk networks were evaluated at different HCC stages,identifying the mT0RC2 component RICTOR as a potential HMGB1 ceRnA in HBV^(+)early stage ***,elevated HMGB1 mRnA was found to promote the expressio n of RICTOR mRnA through competitively bin ding with the miR-200 family,especially *** nal assays emplo ying overexpressi on or in terference strategies dem on strated that the HMGB1 and RICTOR 3zuntra nslated regions(UTR)epigenetically promoted the malignant proliferation,self-renewal,and tumorigenesis in HCC ***,in terference agai nst HMGB1 and RICTOR in HCC cells promoted a stron ger an ti-PD-L1 immuno therapy resp on se,which appeared to associate with the production of PD-L1^(+)***,the HMGB1-driven RnA-RnA crosstalk network facilitated HCC cell glutamine metabolism via dual mechanisms,activating a positive feedback loop involving mT0RC2-AKT-C-MYC to upregulate glutamine synthetase(Gs)expression,and inducing mTORCI signaling to derepress sIRT4 on glutamate dehydrogenase(GDH).Meanwhile,this crosstalk network could impede the efficacy of immunotherapy through mTORCI-P70s6K dependent PD-L1 production and PD-L1^(+)exosomes *** conclusion,our study highlights the non-coding regulatory role of HMGB1 with implicatio ns for RnA-based therapeutic targeting together with a predictio n of an ti-PD-L1 immuno therapy in HCC.

关键词： metabolism network crosstalk

Dynamical Maps of a Class of s_(l+1)=s_(l-1)s_(l)^(n) Fibonacci sequences

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Chinese Physics Letters 1991年第12期8卷 648-651页

作者： ZHAO baohua LIU Tianshi Department of Physics Northeastern University of TechnologyShenyang 110006

The dynamical properties of a class of s_(l+1)=s_(l-1)s_(l)^(n) Fibonacci sequences are discussed following K.K,*** recursion relations for the dynajnical maps are *** about their Gxed points yields the Jacobian matri... 详细信息

关键词： method Fibonacci

不同焊接速度下Cu/Al/Cu纳秒激光焊接接头组织及性能研究

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中国激光 2023年第20期50卷 25-36页

作者：朱宝华牛司成李奇戈赵曙明陆兴檀财旺宋晓国哈尔滨工业大学(深圳)材料科学与工程学院广东深圳518055 深圳市杰普特光电股份有限公司广东深圳518110 哈尔滨工业大学先进焊接与连接国家重点实验室黑龙江哈尔滨150001

采用纳秒脉冲激光对铜/铝/铜三层薄片结构进行焊接,分析焊接速度对焊缝成形、微观组织与接头力学性能的影响。结果表明:较低的焊接速度容易引起气孔和裂纹等接头缺陷,较高的焊接速度造成焊缝中的气孔聚集于界面,合适的焊接速度有利于获... 详细信息

采用纳秒脉冲激光对铜/铝/铜三层薄片结构进行焊接,分析焊接速度对焊缝成形、微观组织与接头力学性能的影响。结果表明:较低的焊接速度容易引起气孔和裂纹等接头缺陷,较高的焊接速度造成焊缝中的气孔聚集于界面,合适的焊接速度有利于获得良好的熔透深度和较少的缺陷;焊缝中存在Cu Al_(2)、Cu_(9)Al_(4)与Cu_(4)Al_(3)等铝/铜二元化合物,且焊缝中生成的化合物的种类与焊接速度有关;当焊接速度为21 mm·s^(-1)时,界面较少的缺陷和钉状焊缝使得接头具有的最大剪切力为95.1 n。接头发生了三种断裂:焊点撕裂、焊缝剪切+熔核拔出复合断裂以及熔核拔出失效,焊点边缘的裂纹及脆性相是焊点撕裂失效的原因,界面处焊缝边缘的铜/铝化合物和气孔缺陷是复合断裂的原因,较浅的熔深和界面化合物导致了熔核拔出失效。

关键词：激光技术纳秒激光焊接 Cu/Al/Cu层状复合接头异种金属薄片焊缝质量

超高效液相色谱-串联质谱法同时测定全血中青霉素G及其主要代谢产物

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色谱 2012年第5期30卷 445-451页

作者：陈聪严慧沈保华卓先义司法部司法鉴定科学技术研究所上海市法医学重点实验室上海200063 上海市疾病预防控制中心上海200336 复旦大学上海医学院法医系上海200032

建立了同时检测全血中青霉素G及其2种主要代谢产物青霉噻唑酸和脱羧青霉噻唑酸的超高效液相色谱-电喷雾串联质谱分析方法。血样经简单的蛋白沉淀提取后,目标化合物经BEH C18色谱柱(50 mm×2.1 mm,1.7μm),以含0.1%甲酸溶液-乙腈为流动相... 详细信息

建立了同时检测全血中青霉素G及其2种主要代谢产物青霉噻唑酸和脱羧青霉噻唑酸的超高效液相色谱-电喷雾串联质谱分析方法。血样经简单的蛋白沉淀提取后,目标化合物经BEH C18色谱柱(50 mm×2.1 mm,1.7μm),以含0.1%甲酸溶液-乙腈为流动相,梯度洗脱分离后,选用正离子电喷雾多反应监测(MRM)模式检测。方法检出限(信噪比(s/n)为3计)为0.1～2.0 ng/mL,定量限(s/n=10)为0.5～5.0 ng/mL。各被测物的线性相关系数均大于0.997 4,准确度为92.3%～105.5%,日内精密度小于10%。考察了不同储存温度(18、4、-18、-80℃)下全血中青霉素G及其代谢物的稳定性,结果表明随着储存温度升高和时间的延长,青霉素G的质量浓度下降明显。应用建立的方法检测了给予20 mg/kg青霉素G后的大鼠血液样本,血液中青霉素G原形药物在给药后0.5 h即消除完全(低于检出限),而其代谢物的体内消除时限可延长至36 h。所建方法对司法鉴定的适用性得到了进一步扩大,同时对食品残留检测也具有一定的参考价值。

关键词：超高效液相色谱-串联质谱青霉素G 代谢产物全血

CO_2激光与喉显微手术治疗声带息肉的疗效对比

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听力学及言语疾病杂志 2002年第1期10卷 19-20页

作者：韩仲明解放军第二炮兵总医院耳鼻咽喉科北京100088

目的比较ＣＯ２激光与喉显微手术治疗声带息肉的治疗效果。方法６０例声带息肉患者分为激光组和喉显微手术组，每组各３０例。患者手术后行间接喉镜声带检查、嗓音声学分析、主观听觉评价分析。结果激光组术后声带形态的恢复时间要长... 详细信息

目的比较ＣＯ２激光与喉显微手术治疗声带息肉的治疗效果。方法６０例声带息肉患者分为激光组和喉显微手术组，每组各３０例。患者手术后行间接喉镜声带检查、嗓音声学分析、主观听觉评价分析。结果激光组术后声带形态的恢复时间要长于喉显微手术组；激光组术后声学参数中的ＰＰＱ值大于而Ｈ／Ｎ值小于显微手术组；激光组术后的嗓音主观听觉评价以粗糙型和紧张型为主，而喉显微手术组仅以粗糙型为主。结论对声带息肉的治疗喉显微手术方法优于激光手术，喉显微手术较激光手术对组织损伤小，术后愈合时间短，术后嗓音恢复快，同时也减轻了患者的经济负担。

关键词：喉息肉喉显微外科 CO2激光声学分析主观听觉评价声带息肉治疗疗效对比

基于圈的紧优双环网络G(n;1,s)求解算法

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华中科技大学学报（自然科学版） 2005年第6期33卷 17-19页

作者：方木云赵保华屈玉贵安徽工业大学计算机学院安徽马鞍山243002 中国科学技术大学计算机系安徽合肥230027

提出基于圈的紧优双环网络G(n;1,s)求解算法,利用VB6.0作为编程语言、sQLserver2000作为数据库来实现这一算法,对任意给定n,而2≤s≤n-1的这样一族双环网络中的所有紧优双环网络都可以计算出来,结果存入数据库.算出n≤200的所有紧优双... 详细信息

提出基于圈的紧优双环网络G(n;1,s)求解算法,利用VB6.0作为编程语言、sQLserver2000作为数据库来实现这一算法,对任意给定n,而2≤s≤n-1的这样一族双环网络中的所有紧优双环网络都可以计算出来,结果存入数据库.算出n≤200的所有紧优双环网络.

关键词：双环网络圈紧优算法族

肝细胞癌患者微创治疗后复发评分系统的建立及验证

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介入放射学杂志 2020年第12期29卷 1207-1212页

作者：陆一峰王祁何忠明苏州大学附属第三医院(常州市第一人民医院)介入放射科江苏常州213000

目的基于肝细胞癌(HCC)患者微创治疗后复发的相关危险因素,建立复发评分系统,并进行验证。方法选取2016年1月1日至2017年12月31日接受经导管动脉化疗栓塞(TACE)联合局部消融治疗的HCC患者160例,根据2年内肿瘤是否复发,分为非复发组(n=79... 详细信息

目的基于肝细胞癌(HCC)患者微创治疗后复发的相关危险因素,建立复发评分系统,并进行验证。方法选取2016年1月1日至2017年12月31日接受经导管动脉化疗栓塞(TACE)联合局部消融治疗的HCC患者160例,根据2年内肿瘤是否复发,分为非复发组(n=79)与复发组(n=81)。通过Cox回归模型分析肿瘤复发的影响因素,根据危险比(HR)构建复发评分系统,并将患者分为低、中、高风险复发组,进行组间临床病理特征及预后比较。结果性别、肿瘤数目、甲胎蛋白(AFP)、纤维蛋白原、白蛋白与前白蛋白比值(APR)是HCC患者肿瘤复发的独立危险因素(均P1年、2年、3年累积无复发生存率(RFs)分别为91.4%、77.1%、68.2%,高于中风险复发组的86.2%、58.6%、48.5%及高风险复发组的50.0%、28.9%、22.3%,组间差异有统计学意义(χ^(2)=12.156,P1)。结论该复发评分系统是TACE联合局部消融治疗HCC患者肿瘤复发的有效预测指标,有利于临床医师做出诊疗决策。

关键词：消融肝细胞癌经导管动脉化疗栓塞评分系统复发

黄芪白术汤加减对便秘患儿结肠传输功能及T淋巴细胞亚群的影响

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光明中医 2021年第14期36卷 2287-2289页

作者：宋保华沈阳市儿童医院心理科辽宁凌源110031

目的研究黄芪白术汤加减对便秘患儿结肠传输功能及T淋巴细胞亚群的影响。方法回顾分析2017年1月—2019年12月接受治疗的148例便秘患儿,按照随机数字表法分为对照组及试验组,均74例。对照组口服乳果糖,试验组在此基础上联合黄芪白术汤加... 详细信息

目的研究黄芪白术汤加减对便秘患儿结肠传输功能及T淋巴细胞亚群的影响。方法回顾分析2017年1月—2019年12月接受治疗的148例便秘患儿,按照随机数字表法分为对照组及试验组,均74例。对照组口服乳果糖,试验组在此基础上联合黄芪白术汤加减治疗,4周为一个疗程,2组患儿均连续治疗2个疗程。比较2组患儿治疗后的临床疗效,治疗前后结肠传输功能和T淋巴细胞亚群水平变化。结果试验组患儿总有效率为95.95%,高于对照组的78.38%;治疗后试验组较对照组结肠排空时间均缩短;与治疗前相比,治疗后2组患儿外周血CD8^(+)水平降低,且试验组低于对照组;2组患儿外周血CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)均升高,且试验组高于对照组(P<0.05)。结论黄芪白术汤加减可显著改善便秘患儿结肠传输功能,同时调节T淋巴细胞亚群水平,提升免疫功能,提高治疗效果。

关键词：便秘黄芪白术汤中医药疗法中医儿科学

YTHDF2-mediated regulations bifurcate BHPF-induced programmed cell deaths

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national science Review 2023年第12期10卷 83-97页

作者： Jiebo Lin Guankai Zhan Jinfeng Liu Yasen Maimaitiyiming Zhiping Deng baohua Li Kunhui su Jiafeng Chen siqi sun Wanlin Zheng Xianghui Yu Feng He Xiaodong Cheng Lingfang Wang Bin shen Ziqin Yao Xinquan Yang Jian Zhang Wentao He Hengyu Wu Hua naranmandura Kao-Jung Chang Junxia Min Jun Ma Mikael Bj?rklund Peng-Fei Xu Fudi Wang Chih-Hung Hsu Women's Hospital The Fourth Affiliated Hospitaland Department of Environmental MedicineZhejiang University School of Medicine Institute of Genetics International School of MedicineZhejiang University The second Affiliated Hospital School of Public Health State Key Laboratory of Experimental Hematology Zhejiang University School of Medicine Department of Hematology of First Affiliated Hospital and Department of Public Health Zhejiang University School of Medicine state Key Laboratory for Managing Biotic and Chemical Threats to the Quality and safety of Agro-products Institute of Virology and Biotechnology Zhejiang Academy of Agricultural Sciences Department of Obstetrics Women's Hospital School of Medicine Zhejiang University Women's Hospital and Institute of Genetics Zhejiang University School of Medicine Zhejiang Provincial Key Lab of Genetic and Developmental Disorders Zhejiang Provincial Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine state Key Laboratory of Reproductive Medicine Center for Global HealthGusu School Women's Hospital of Nanjing Medical University Nanjing Maternity and Child Health Care Hospital Nanjing Medical University The First Affiliated Hospital Basic Medical SciencesSchool of Public HealthSchool of Pharmaceutical Science Hengyang Medical School University of South China Department of Medical Research Taipei Veterans General Hospital The First Affiliated Hospital Institute of Translational MedicineCancer Center Zhejiang University School of Medicine Zhejiang University-University of Edinburgh (ZJU-UoE) Institute University of Edinburgh Medical school Biomedical SciencesCollege of Medicine & Veterinary MedicineUniversity of Edinburgh

n6-methyladenosine(m6A) is a critical regulator in the fate of RnA, but whether and how m6A executes its functions in different tissues remains largely obscure. Here we report downregulation of a crucial m6A reader, YTHDF2, leading to tissue-specific programmed cell deaths(PCDs) upon fluorene-9-bisphenol(BHPF) exposure. Currently, Bisphenol A(BPA) substitutes are widely used in plastic *** eight common BPA substitutes, we detected BHPF in 14% serum samples of pregnant participants. In a zebrafish model, BHPF caused tissue-specific PCDs triggering cardiac and vascular ***, BHPF-mediated downregulation of YTHDF2 reduced YTHDF2-facilitated translation of m6A-gch1 for cardiomyocyte ferroptosis, and decreased YTHDF2-mediated m6A-sting1 decay for caudal vein plexus(CVP) apoptosis. The two distinct YTHDF2-mediated m6A regulations and context-dependent co-expression patterns of gch1/ythdf2 and tnfrsf1a/ythdf2 contributed to YTHDF2-mediated tissue-specific PCDs, uncovering a new layer of PCD regulation. since BHPF/YTHDF2-medaited PCD defects were also observed in mammals, BHPF exposure represents a potential health threat.

关键词： RnA m6A modification programmed cell deaths bifurcation role of YTHDF2 environmental stress and m6A regulation fluorene-9-bisphenol (BHPF)