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A direct link between BR and SA signaling: Negative regulation of TGA4 by BIN2

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Molecular Plant 2022年第8期15卷 1254-1256页

作者： Cheng Liu Qingcai Liu zhonglin mou Department of Microbiology and Cell Science University of FloridaP.O.Box 110700GainesvilleFL32611USA

It is well known that plantgrowth,development,and response to environmental stresses are harmonized through theaction of a suite of growth and defense signaling ***(BR)is a steroid hormone that regulates plant growth ... 详细信息

关键词： BIN2 development. plant

Monitoring gene expression by cDNA array

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Chinese Science Bulletin 1999年第5期44卷 441-444页

作者： Yuxin Hu Chang Han zhonglin mou Jiayang Li Chinese Acad Sci Inst Genet Beijing 100101 Peoples R China DeMontfort Univ Beijing Ctr Norman Borlaug Inst Bot Leicester Leics England

A new method designated cDNA array was developed by hybridization of quantitatively arrayed DNA samples isolated randomly from a cDNA library with probes reverse-transcribed from mRNAs of different sources or treatments. The gene expression patterns of 1 000 randomly chosen clones from an Arabidopsis library were analyzed with green seedlings versus suspension cells and seedlings irradiated under UV light. Northern blot and sequence analysis of some differentially expressed clones confirmed the results revealed by cDNA array, indicating that this method is efficient and reliable to monitor gene expression.

关键词： gene expression cDNA array functional genomics Arabidopsis thaliana.

NPR1 as a transgenic crop protection strategy in horticultural species

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Horticulture Research 2018年第1期5卷 751-761页

作者： Katchen Julliany P.Silva Nasser Mahna zhonglin mou Kevin M.Folta Horticultural Sciences Department University of FloridaGainesvilleFL32611USA Department of Horticultural Sciences University of TabrizTabrizIran Department of Microbiology and Cell Science University of FloridaGainesvilleFL32611USA Graduate Program in Plant Molecular and Cellular Biology University of FloridaGainesvilleFL32611USA

The NPR1(NONEXPRESSOR OF PATHOGENESIS RELATED GENES1)gene has a central role in the long-lasting,broad-spectrum defense response known as systemic acquired resistance(SAR).When overexpressed in a transgenic context in Arabidopsis thaliana,this gene enhances resistance to a number of biotic and abiotic *** position as a key regulator of defense across diverse plant species makes NPR1 a strong candidate gene for genetic engineering disease and stress tolerance into other ***-value horticultural crops face many new challenges from pests and pathogens,and their emergence exceeds the pace of traditional breeding,making the application of NPR1-based strategies potentially useful in fruit and vegetable ***,plants overexpressing NPR1 occasionally present detrimental morphological traits that make its application less *** practical utility of NPR-based approaches will be a balance of resistance gains versus other *** this review,we summarize the progress on the understanding of NPR1-centered applications in horticultural and other crop *** also discuss the effect of the ectopic expression of the *** NPR1 gene and its orthologs in crop plants and outline the future challenges of using NPR1 in agricultural applications.

关键词： NPR1 breeding attractive

An Efficient Intragenic Vector for Generating Intragenic and Cisgenic Plants in Citrus

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American Journal of Plant Sciences 2013年第11期4卷 2131-2137页

作者： Chuanfu An Vladimir Orbovic zhonglin mou Department of Microbiology and Cell Science University of FloridaGainesvilleUSA Citrus Research and Education Center University of FloridaLake AlfredUSA

Genetic transformation has become a promising tool for improvement of a variety of crop species. However, transferring genes across species, the presence of selectable marker genes, and bacteria-derived vector backbone sequences have raised considerable health and environmental concerns. Intragenic vector system-based intragenesis/cisgenesis is a new method using transgenic approach to achieving traditional breeding objectives but circumventing many of the associated shortcomings. We report here the development of an intragenic vector by assembling a T-DNA-like fragment and a buffering sequence following the left border from Citrus clementina into the backbone of the binary vector pCB302. Recovery of citrus regenerants is performed under non-selective conditions and positive intra-/cisgenic regenerants were identified through PCR analysis. Transformation efficiencies obtained in Arabidopsis and “Duncan” grapefruit were ~3% and ~0.67%, respectively, demonstrating the potential of the system for development of “foreign DNA-free” intra-/cisgenic citrus cultivars.

关键词： Intragenesis/Cisgenesis Citrus Selectable Marker Transgenesis

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变应性鼻炎免疫治疗的研究进展

免疫学杂志 2018年第1期34卷 86-92页

作者：熊斯怡杨洋阮昊牟忠林海南医学院临床学院 2014级本科571199 海南医学院第一附属医院耳鼻咽喉头颈外科 571199

变应性鼻炎是一种由变应原激发并通过Ig E介导的鼻黏膜炎性疾病,因其反复发作的鼻部症状和并发症,给临床治疗带来很大挑战。免疫治疗是目前唯一能够改变变应性鼻炎自然病程、针对病因的治疗方法。近来,包括新的给药途径、变应原改造在... 详细信息

变应性鼻炎是一种由变应原激发并通过Ig E介导的鼻黏膜炎性疾病,因其反复发作的鼻部症状和并发症,给临床治疗带来很大挑战。免疫治疗是目前唯一能够改变变应性鼻炎自然病程、针对病因的治疗方法。近来,包括新的给药途径、变应原改造在内的各种新型免疫治疗方法大量涌现,给变应性鼻炎治疗提供更多有效和安全的选择。本文针对变应性鼻炎免疫治疗的最新研究进展进行综述,并对未来的方向进行展望。

关键词：变应性鼻炎免疫治疗变应原

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原发性头颈部非霍奇金淋巴瘤临床分析

中国耳鼻咽喉头颈外科 2007年第12期14卷 695-697页

作者：谭业农牟忠林赵质彬符征海南省人民医院耳鼻咽喉头颈外科海南海口570311

目的探讨原发于头颈部的非霍奇金淋巴瘤(non-Hodgkin's lymphoma,NHL)的临床及病理特点,为早期诊断提供经验,从而降低误诊率及漏诊率。方法对51例原发于头颈部,经病理确诊为NHL患者的临床资料进行回顾性分析。结果原发部位,鼻腔、鼻窦19... 详细信息

目的探讨原发于头颈部的非霍奇金淋巴瘤(non-Hodgkin's lymphoma,NHL)的临床及病理特点,为早期诊断提供经验,从而降低误诊率及漏诊率。方法对51例原发于头颈部,经病理确诊为NHL患者的临床资料进行回顾性分析。结果原发部位,鼻腔、鼻窦19例(37.3%),腭扁桃体14例(27.5%),颈淋巴结8例(15.7%),鼻咽部6例(11.8%)。误诊为炎症7例(13.7%),鼻咽癌2例(3.9%),总误诊率17.6%。结论头颈部NHL恶性程度高,多好发于鼻腔及鼻窦,其次为扁桃体。该病误诊率高,准确的取材活检及免疫组化检查至关重要。

关键词：头颈部肿瘤非霍奇金淋巴瘤误诊

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自发性脑脊液鼻漏临床分析

中国耳鼻咽喉头颈外科 2009年第2期16卷 58-61页

作者：赵质彬牟忠林符征谭业农海南省人民医院耳鼻咽喉头颈外科海南海口570311

目的探讨自发性脑脊液鼻漏的诊治方法。方法回顾性分析13例自发性脑脊液鼻漏的临床特征、影像学特点及诊治方法与疗效。结果13例自发性脑脊液鼻漏患者,通过询问病史,收集鼻漏出液行葡萄糖定性定量检测,鼻内镜检查和CT、MRI影像学检查均... 详细信息

目的探讨自发性脑脊液鼻漏的诊治方法。方法回顾性分析13例自发性脑脊液鼻漏的临床特征、影像学特点及诊治方法与疗效。结果13例自发性脑脊液鼻漏患者,通过询问病史,收集鼻漏出液行葡萄糖定性定量检测,鼻内镜检查和CT、MRI影像学检查均得以确诊,保守治疗无效,均行鼻内镜下修补术,术后随访6个月～6年,均无复发,无脑膜炎等并发症,手术修补成功率100%。结论葡萄糖定性定量检测、鼻内镜检查及CT、MRI影像学检查是诊断自发性脑脊液鼻漏的主要方法,鼻内镜下寻找漏口及修补术具有微创、并发症少及成功率高等优点,是自发性脑脊液鼻漏的首选治疗方法。

关键词：脑脊液鼻漏诊断内窥镜检查

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鼻源性分泌性中耳炎的诊断及治疗

临床耳鼻咽喉头颈外科杂志 2011年第7期25卷 301-302,306页

作者：林杰牟忠林况光仪易慧明海南省人民医院耳鼻咽喉头颈外科海口570311

目的:通过对176例因鼻腔结构异常所引起的分泌性中耳炎的诊断和治疗,提出了鼻源性中耳炎的概念,同时阐述了鼻源性中耳炎的发病机制。方法:对伴随有鼻腔结构异常的176例鼻源性中耳炎患者分别进行了内科保守治疗及鼻内镜下相关手术治疗。... 详细信息

目的:通过对176例因鼻腔结构异常所引起的分泌性中耳炎的诊断和治疗,提出了鼻源性中耳炎的概念,同时阐述了鼻源性中耳炎的发病机制。方法:对伴随有鼻腔结构异常的176例鼻源性中耳炎患者分别进行了内科保守治疗及鼻内镜下相关手术治疗。结果:痊愈156例(88.64%),有效18例(10.23%),无效2例(1.14%)。结论:鼻腔结构异常是分泌性中耳炎发生的重要原因,手术矫正鼻腔结构异常是治疗鼻源性中耳炎的主要方法之一。

关键词：中耳炎鼻腔结构异常诊断

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叉头状转录因子3在变应性鼻炎中的表达

中国耳鼻咽喉头颈外科 2007年第6期14卷 375-378页

作者：牟忠林赵质彬李华斌谭业农程雷海南省人民医院耳鼻咽喉科海南海口570311 南京医科大学第一附属医院江苏省人民医院耳鼻咽喉科江苏南京210029

目的探讨叉头状转录因子p3(forkhead transcription factor p3,Foxp3)在常年性变应性鼻炎发病中的作用。方法采用免疫组化SABC法和实时定量逆转录聚合酶链反应(reverse transcription polymerase reaction,RT-PCR)分别从蛋白和基因水平上检测了17例常年性变应性鼻炎患者和11例正常对照者鼻黏膜中Foxp3的表达。同时采用流式细胞仪检测了外周血单个核细胞中Foxp3的表达情况和CD4+CD25+T细胞的阳性比例。结果变应性鼻炎患者和对照者鼻黏膜中Foxp3阳性细胞数分别为(44.2±20.5)个/mm2和(129.5±35.6)个/mm2(P<0.05),外周血单个核细胞中Foxp3平均荧光强度为5.93±2.64和11.72±4.29(P<0.05),CD4+CD25+T细胞分别为(1.99±0.95)%和(3.55±1.27)%(P<0.05),均具有显著性差异。结论Foxp3表达降低和CD4+CD25+T细胞比例下降可能对变应性鼻炎的发生和治疗具有重要意义。

关键词：鼻炎变应性常年性转录因子 T 淋巴细胞