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Construction and characterization of the transformation-competent artificial chromosome (TAC) libraries of Leymus multicaulis

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Science China(Life Sciences) 2008年第7期51卷 604-613页

作者： XU YueYu 1,2,3,ZHOU YuLei 4,5 ,SONG LinLin 6 ,ZHANG Yan 3 &ZHAO MaoLin 1 1 Beijing Agro-Biotechnology Research Center,Beijing Academy of agriculture and Forestry Science,Beijing 100097,China 2 Department of Chemistry and Chemical Engineering,Hunan Institute of Engineering,Xiangtan 411104,China 3 College of Life Science,Capital Normal university,Beijing 100037,China 4 College of Life Science,zhongkai university of agriculture and technology,Guangzhou 510225,China 5 College of Grassland Science,Gansu Agricultural university,Lanzhou 730070,China 6 Department of Biology,Henan Institute of Science and technology,Xinxiang 453003,China 1. Beijing Agro-Biotechnology Research Center Beijing Academy of Agriculture and Forestry Science Beijing 100097 China 2. Department of Chemistry and Chemical Engineering Hunan Institute of Engineering Xiangtan 411104 China 3. College of Life Science Capital Normal University Beijing 100037 China 4. College of Life Science Zhongkai University of Agriculture and Technology Guangzhou 510225 China 5. College of Grassland Science Gansu Agricultural University Lanzhou 730070 China 6. Department of Biology Henan Institute of Science and Technology Xinxiang 453003 China

Transformation-competent artificial chromosome system is able to clone and transfer genes efficiently in *** order to clone genes highly tolerant to barley yellow dwarf virus(BYDV),Aphids,drought and salt from Leymus multicaulis,the two TAC genomic libraries I and II were constructed in vector pYLTAC17 and pYLTAC747H/sacB,which contain about 165000 and 236000 recombinant clones *** genome coverage of the two libraries was totally estimated to be about 3―5 haploid genome equivalents,as size selection of genomic DNA fragments was approximately from 9 to 300 *** of the genomic libraries were collected as bulked pools each containing 500 clones or so,stored in twelve 96-deep-well plates and then were gridding in triplicate onto a high-density colony hybridization filter with a 3×3 pattern using a GeneTAC?G3 arraying robot after being transferred manually into three 384-well *** 2501 and 2890 clones of Library in pYLTAC17 and in pYLTAC747H/sacB were stored individually in fourteen 384-well plates and then were automatically gridding in duplicate onto a high-density colony hybridization filter with a 6×6 pattern after a replication of *** positive clones were detected by using the probe glutahione reductase gene of *** libraries constructed here can be used to isolate genomic clones containing target genes,and to carry out genome walking for positional *** the target TAC clones were isolated,they could be immediately transferred into plant genomes with the Agrobacterium system.

关键词： Leymus multicaulis megabase-size DNA transformation-competent artificial chromosome(TAC) genomic library

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论认知隐喻学:两个“意义”的区分

外语学刊 2013年第3期 73-77页

作者：熊学军仲恺农业工程学院广州510225 上海外国语大学上海200083

文章根据语言与思维的关系理清认知隐喻学的意义观,提出将隐喻概念及其意义与隐喻话语及其意义区分开来、给出定义及讨论,从而把握认知隐喻学意义观的得失处,明确借鉴该理论的深层含义。

关键词：意义隐喻概念隐喻话语

Green turf quality and evapotranspiration rate in summer of Agrostis stolonifera *** No.1 in Guangzhou area

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Green turf quality and evapotranspiration rate in summer of ...

ⅩⅪ International Grassland Congress and the Ⅷ International Rangeland Congress

作者： PChen X L Shi P Liu Y L Zhou A Liu Department of Pratacultural science College of agriculture and Gardening zhongkai university of agriculture and technology Guangzhou Guangdong 510225 PR China

＜正＞Introduction In order to enhance the stability of high temperature and high humidity stress and construct evergreen green in Guangzhou area,we researched traits of Agrostis stolonifera *** No.1 and *** character... 详细信息

关键词： Agrostis stolonifera L.cv.Yuexuan No.1 golf green turf quality growth speed evapotranspiration rate

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cnki会议

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脂肪替代品对脱脂搅拌型酸奶流变性的影响

食品科技 2005年第1期30卷 71-73页

作者：成坚刘晓艳王琴仲恺农业技术学院食品系广州510225

研究不同脂肪替代品对脱脂搅拌型酸奶流变性的影响。实验表明:适当浓度的海藻酸丙二醇酯(PGA)、琼脂、高酯果胶、明胶、变性淀粉,都可以使酸奶达到一定的表观黏度,具有增稠的作用,从而解决酸奶组织砂状和口感粗糙、乳清分离等问题。其... 详细信息

研究不同脂肪替代品对脱脂搅拌型酸奶流变性的影响。实验表明:适当浓度的海藻酸丙二醇酯(PGA)、琼脂、高酯果胶、明胶、变性淀粉,都可以使酸奶达到一定的表观黏度,具有增稠的作用,从而解决酸奶组织砂状和口感粗糙、乳清分离等问题。其中添加0.50%变性淀粉及0.10%果胶的抗剪切能力最好。

关键词：脂肪替代品搅拌型酸奶流变性

ARPES investigation of the electronic structure and its evolution in magnetic topological insulator MnBi2+2nTe4+3n family

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National Science Review 2024年第2期11卷 81-93页

作者： Runzhe Xu Lixuan Xu zhongkai Liu Lexian Yang Yulin Chen State Key Laboratory of Low Dimensional Quantum Physics Department of PhysicsTsinghua University School of Physical Science and technology Shanghai Tech University and CAS-Shanghai Science Research Center Shanghai Tech Laboratory for Topological Physics Frontier Science Center for Quantum Information Collaborative Innovation Center of Quantum Matter Department of Physics Clarendon LaboratoryUniversity of Oxford

In the past 5 years,there has been significant research interest in the intrinsic magnetic topological insulator family compounds MnBi2+2nTe4+3n（where n=0,1,2…）.In particular,exfoliated thin films of MnBi2Te4have led to numerous experimental breakthroughs,such as the quantum anomalous Hall effect,axion insulator phase and high-Chern number quantum Hall effect without Landau ***,despite extensive efforts,the energy gap of the topological surface states due to exchange magnetic coupling,which is a key feature of the characteristic band structure of the system,remains experimentally *** electronic structure measured by using angle-resolved photoemission （ARPES） shows significant deviation from ab initio prediction and scanning tunneling spectroscopy measurements,making it challenging to understand the transport results based on the electronic *** paper reviews the measurements of the band structure of MnBi2+2nTe4+3nmagnetic topological insulators using ARPES,focusing on the evolution of their electronic structures with temperature,surface and bulk doping and film *** aim of the review is to construct a unified picture of the electronic structure of MnBi2+2nTe4+3ncompounds and explore possible control of their topological properties.

关键词： magnetic topological insulator MnBi2Te4 electronic structure angle-resolved photoemission spectroscopy

Pulsed current-assisted twelve-roll precision rolling deformation of SUS304 ultra-thin strips with exceptional mechanical properties

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International Journal of Extreme Manufacturing 2024年第4期6卷 291-305页

作者： Wanwan Fan Tao Wang Jinxiong Hou zhongkai Ren Qingxue Huang Guanghui Wu College of Mechanical and Vehicle Engineering Taiyuan University of TechnologyYingze West Street 79Taiyuan 030024Shanxi ProvincePeople's Republic of China Engineering Research Center of Advanced Metal Composites Forming technology and Equipment of Ministry of Education Taiyuan University of TechnologyYingze West Street 79Taiyuan 030024Shanxi ProvincePeople's Republic of China National Key Laboratory of Metal Forming technology and Heavy Equipment Taiyuan University of TechnologyYingze West Street 79Taiyuan 030024Shanxi ProvincePeople's Republic of China College of Aeronautics and Astronautics Taiyuan University of TechnologyYingze West Street 79Taiyuan 030024Shanxi ProvincePeople's Republic of China

Innovative pulsed current-assisted multi-pass rolling tests were conducted on a 12-roll mill during the rolling deformation processing of SUS304 ultra-thin *** results show that in the first rolling pass,the rolling reduction rate of a conventionally rolled sample(at room temperature)is 33.8%,which can be increased to 41.5%by pulsed current-assisted rolling,enabling the formation of an ultra-thin strip with a size of 67.3μm in only one rolling *** three passes of pulsed current-assisted rolling,the thickness of the ultra-thin strip can be further reduced to 51.7μ*** clearly compare the effects of a pulsed current on the microstructure and mechanical response of the ultra-thin strip,ultra-thin strips with nearly the same thickness reduction were *** was found that pulsed current can reduce the degree of work-hardening of the rolled samples by promoting dislocation detachment,reducing the density of stacking faults,inhibiting martensitic phase transformation,and shortening the total length of grain *** a result,the ductility of ultra-thin strips can be effectively restored to approximately 16.3%while maintaining a high tensile strength of 1118 ***,pulsed current-assisted rolling deformation shows great potential for the formation of ultra-thin strips with a combination of high strength and ductility.

关键词： pulsedcurrent-assisted SUS304 ultra-thinstrip rolling reductionrate work-hardening mechanical properties

OSCILLATIONS OF IMPULSES DELAY DIFFERENTIAL EQUATIONS WITH FORCING TERM

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Annals of Differential Equations 2006年第1期22卷 95-100页

作者： Zhang Chaolong Yang Jianfu Guo Chengjun Dept. of Computation Science Zhongkai University of Agriculture and Technology Guangzhou 510225 Dept. of Math. South China Normal University Guangzhou 510631 Dept. of Basic Science Guangdong Technical College of Water Resource and Electric Engineering Guangzhou 510631

A kind of extended first order impulses nonlinear FDE with forcing term is studied in this paper. Several criteria on the oscillations of solutions are given. We find some suitable impulse functions such that all the ... 详细信息

关键词： impulses FDE oscillation forcing term

A folic acid-decorated nanoparticles loaded JQ1 for oral squamous cell carcinoma therapy

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Chinese Chemical Letters 2024年第3期35卷 332-336页

作者： Dequan Zeng zhongkai Ma Xin Zan Ting Luo Xiang Wang Xiang Gao Xianghui Fu School of Health Management Xihua UniversityChengdu 610039China State Key Laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesWest China College of StomatologySichuan UniversityChengdu 610041China Department of Neurosurgery and Institute of Neurosurgery State Key Laboratory of Biotherapy and Cancer CenterWest China HospitalWest China Medical SchoolSichuan University and Collaborative Innovation Center for BiotherapyChengdu 610041China Breast Disease Center Cancer CenterWest China HospitalSichuan UniversityChengdu 610041China

Oral squamous cell carcinoma(OSCC)is known as one of the most malignant tumors with high recurrence and fatality *** poor tumor-targeting ability of traditional chemotherapeutic drugs has been a grand challenge for anti-OSCC *** that,a large quantity of tumor associated macrophages in OSCC tissues further diminish the anti-tumor effects of these ***,we produced a therapeutic nano drug delivery system(FA-PEG-PLA-JQ1)through encapsulating JQ1[a small-molecule inhibitor of bromodomain containing protein 4(BRD4)]into the folic acid(FA)-modified nanoparticle(PEG-PLA),which could prolong the half-life of JQ1 and target the tumor *** then,JQ1 released from this nanoparticle could prevent OSCC growth inducing tumor cell apoptosis,inhibiting tumor angiogenesis and the polarization of M2 type *** conclusion,our date demonstrated the therapeutic benefits of FA-PEG-PLA-JQ1 against OSCC in vivo or in vitro,which could be a novel treatment strategy for OSCC in coming days.

关键词： Folic acid Nanoparticles JQ1 Oral squamous cell carcinoma Macrophages

Enabling superior electrochemical performance of NCA cathode in Li_(5.5)PS_(4.5)Cl_(1.5)-based solid-state batteries with a dual-electrolyte layer

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Chinese Chemical Letters 2024年第4期35卷 231-236页

作者： Ziling Jiang Shaoqing Chen Chaochao Wei Ziqi Zhang zhongkai Wu Qiyue Luo Liang Ming Long Zhang Chuang Yu School of Chemistry and Chemical Engineering Huazhong University of Science and TechnologyWuhan 430074China State Key Laboratory of Advanced Electromagnetic Engineering and technology School of Electrical and Electronic EngineeringHuazhong University of Science and TechnologyWuhan 430074China Innovation Center for Chemical Sciences College of ChemistryChemical Engineering and Materials ScienceSoochow UniversitySuzhou 215123China College of Physics and Energy Fujian Normal UniversityFuzhou 350117 China

LiNi_(0.8)Co_(0.15)Al_(0.05)O_(2)(NCA) is a promising cathode for sulfide-based solid-state lithium batteries(ASSLBs)profiting from its high specific capacity and voltage plateau, which yielding high energy density. However, the inferior interfacial stability between the bare NCA and sulfides limits its electrochemical performance. Hereien, the dual-electrolyte layer is proposed to mitigate this effect and enhance the battery performances of NCA-based ASSLIBs. The Li_(3)InCl_6 wih high conductivity and excellent electrochemcial stability act both as an ion additives to promote Li-ion diffusion across the interface in the cathode and as a buffer layer between the cathode layer and the solid electrolyte layer to avoid side reactions and improve the interface stability. The corresponding battery exhibits high discharge capacities and superior cyclabilities at both room and elevated temperatures. It exhibits discharge performance of 237.04 and216.07 m Ah/g at 0.1 and 0.5 C, respectively, when cycled at 60 ℃, and sustains 95.9% of the capacity after100 cycles at 0.5 C. The work demonstrates a simple strategy to ensure the superior performances of NCA in sulfide-based ASSLBs.

关键词： LiNi_(0.8)Co_(0.15)Al_(0.05)O_(2) Li_(5.5)PS_(4.5)Cl_(1.5) Double solid electrolyte layer configuration Operating temperatures Electrochemical performances