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Population genomics highlights structural variations in local adaptation to saline coastal environments in woolly grape

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Journal of Integrative Plant Biology 2024年第7期66卷 1408-1426页

作者： Tianhao Zhang Wenjing Peng Hua Xiao Shuo Cao Zhuyifu Chen Xiangnian Su Yuanyuan Luo zhongjie liu Yanling Peng Xiping Yang Guo-Feng Jiang Xiaodong Xu Zhiyao Ma Yongfeng Zhou National Key Laboratory of Tropical Crop Breeding Shenzhen BranchGuangdong Laboratory of Lingnan Modern AgricultureKey Laboratory of Synthetic BiologyMinistry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518000China State Key Laboratory for Conservation and Utilization of Subtropical Agro‐Bioresources Guangxi UniversityNanning 530004China Guangxi Key Laboratory of Forest Ecology and Conservation Guangxi Colleges and Universities Key Laboratory for Cultivation and Utilization of Subtropical Forest PlantationCollege of ForestryGuangxi UniversityNanning 530004China College of Informatics Huazhong Agricultural UniversityWuhan 430070China Guangxi Key Laboratory of Sugarcane Biology College of AgricultureGuangxi UniversityNanning 530004China Key Laboratory of Horticultural Plant Biology Ministry of Education Huazhong Agricultural UniversityWuhan 430070China Zhengzhou Fruit Research Institute Chinese Academy of Agricultural SciencesZhengzhou 450009China National Key Laboratory of Tropical Crop Breeding Tropical Crops Genetic Resources InstituteChinese Academy of Tropical Agricultural SciencesHaikou 571101China

Structural variations(SVs)are a feature of plant genomes that has been largely unexplored despite their significant impact on plant phenotypic traits and local adaptation to abiotic and biotic *** this study,we employed woolly grape(Vitis retordii),a species native to the tropical and subtropical regions of East Asia with both coastal and inland habitats,as a valuable model for examining the impact of SVs on local *** assembled a haplotype-resolved chromosomal reference genome for woolly grape,and conducted population genetic analyses based on whole-genome sequencing(WGS)data from coastal and inland *** demographic analyses revealed recent bottlenecks in all populations and asymmetric gene flow from the inland to the coastal *** total,1,035 genes associated with plant adaptive regulation for salt stress,radiation,and environmental adaptation were detected underlying local selection by SVs and SNPs in the coastal population,of which 37.29% and 65.26% were detected by SVs and SNPs,*** genes such as FSD2,RGA1,and AAP8 associated with salt tolerance were found to be highly differentiated and selected during the process of local adaptation to coastal habitats in SV *** study highlights the importance of SVs in local adaptation;candidate genes related to salt stress and climatic adaptation to tropical and subtropical environments are important genomic resources for future breeding programs of grapevine and its rootstocks.

关键词： climate change grape breeding local adaptation with gene flow salt tolerance viticulture Vitis

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Evolutionary genomics of climatic adaptation and resilience to climate change in alfalfa

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Molecular Plant 2024年第6期17卷 867-883页

作者： Fan Zhang Ruicai Long Zhiyao Ma Hua Xiao Xiaodong Xu zhongjie liu Chunxue Wei Yiwen Wang Yanling Peng Xuanwen Yang Xiaoya Shi Shuo Cao Mingna Li Ming Xu Fei He Xueqian Jiang Tiejun Zhang Zhen Wang Xianran Li Long-Xi Yu Junmei Kang Zhiwu Zhang Yongfeng Zhou Qingchuan Yang Institute of Animal Science Chinese Academy of Agricultural SciencesBeijing 100193China National Key Laboratory of Tropical Crop Breeding Shenzhen BranchGuangdong Laboratory of Lingnan Modern AgricultureKey Laboratory of Synthetic BiologyMinistry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518000China School of Grassland Science Beijing Forestry UniversityBeijing 100083China Department of Agronomy and Horticulture University of Nebraska-LincolnLincolnNE 68583USA Department of Crop and Soil Sciences Washington State UniversityPullmanWA 99163USA U.S.Department of Agriculture-Agricultural Research Service Plant Germplasm Introduction and Testing ResearchProsserWA 99350USA National Key Laboratory of Tropical Crop Breeding Tropical Crops Genetic Resources InstituteChinese Academy of Tropical Agricultural SciencesHaikou 571101China

Given the escalating impact of climate change on agriculture and food security,gaining insights into the evolutionary dynamics of climatic adaptation and uncovering climate-adapted variation can empower the breeding of climate-resilient crops to face future climate ***(Medicago sativa ***),the queen of forages,shows remarkable adaptability across diverse global environments,making it an excellent model for investigating species responses to climate *** this study,we performed population genomic analyses using genome resequencing data from 702 accessions of 24 Medicago species to unravel alfalfa’s climatic adaptation and genetic susceptibility to future climate *** found that interspecific genetic exchange has contributed to the gene pool of alfalfa,particularly enriching defense and stress-response *** introgression between *** ***(***)and alfalfa not only aids alfalfa’s climatic adaptation but also introduces genetic burden.A total of 1671 genes were associated with climatic adaptation,and 5.7%of them were introgressions from *** integrating climate-associated variants and climate data,we identified populations that are vulnerable to future climate change,particularly in higher latitudes of the Northern *** findings serve as a clarion call for targeted conservation initiatives and breeding *** also identified preadaptive populations that demonstrate heightened resilience to climate fluctuations,illuminating a pathway for future breeding ***,this study enhances our understanding about the local adaptation mechanisms of alfalfa and facilitates the breeding of climate-resilient alfalfa cultivars,contributing to effective agricultural strategies for facing future climate change.

关键词： Medicago local adaptation population genetics adaptive introgression genetic vulnerability alfalfa breeding

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视频弹幕评论舆情分析系统设计与实现

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常州信息职业技术学院学报 2024年第4期23卷 20-26页

作者：刘忠杰常州信息职业技术学院软件与大数据学院江苏常州213164

随着用户生成内容视频网站的普及,弹幕评论成为用户表达情感性意见的重要方式。弹幕评论的即时性和互动性为网络舆情分析提供了丰富的数据资源,但其匿名性和短文本的特点给数据分析带来一定的困难。设计视频弹幕评论网络舆情分析系统,... 详细信息

随着用户生成内容视频网站的普及,弹幕评论成为用户表达情感性意见的重要方式。弹幕评论的即时性和互动性为网络舆情分析提供了丰富的数据资源,但其匿名性和短文本的特点给数据分析带来一定的困难。设计视频弹幕评论网络舆情分析系统,能够有效识别和过滤异常用户,并进行情感分析,最终完成对弹幕评论的主题聚类和可视化展示。系统采用前后端分离架构,前端基于Vue框架,后端使用Django框架,数据存储采用MySQL数据库。通过对Bilibili视频网站弹幕数据的实验分析,验证了系统的有效性和稳定性,促进了网络舆情分析系统的发展。

关键词：弹幕评论网络舆情分析情感分析主题聚类

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景明散临床应用举隅

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中国兽医杂志 2024年第7期60卷 122-123页

作者：钟沅胡宇声刘钟杰中国农业大学动物医学院北京海淀100193 中国农业大学动物医院北京海淀100193

景明散是笔者根据犬猫临床实际结合用药经验总结出的验方。该方由生黄芪、生白术、补骨脂三味药组成,适用于以气阳虚为主要病机的多种疾病。本文介绍了景明散在治疗犬胃肠炎和猫哮喘,以及老年犬多病共存调养中的运用,以期为临床处理犬... 详细信息

景明散是笔者根据犬猫临床实际结合用药经验总结出的验方。该方由生黄芪、生白术、补骨脂三味药组成,适用于以气阳虚为主要病机的多种疾病。本文介绍了景明散在治疗犬胃肠炎和猫哮喘,以及老年犬多病共存调养中的运用,以期为临床处理犬猫气虚、阳虚病症提供组方思路和用药参考。

关键词：景明散中兽医用药经验

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基于Lasso-Logistic回归分析子宫颈高度鳞状上皮内病变锥切术后切缘阳性及病灶残留的影响因素

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中国性科学 2024年第10期33卷 96-100页

作者：杜素果宋永祯刘忠杰赵宁衡水市人民医院妇科河北衡水053000

目的探讨基于Lasso-Logistic回归分析子宫颈高度鳞状上皮内病变(HSIL)锥切术后切缘阳性及病灶残留的影响因素。方法选取2021年7月至2022年1月衡水市人民医院收治的160例子宫颈HSIL患者作为研究对象,均行宫颈锥切术治疗。根据是否存在锥... 详细信息

目的探讨基于Lasso-Logistic回归分析子宫颈高度鳞状上皮内病变(HSIL)锥切术后切缘阳性及病灶残留的影响因素。方法选取2021年7月至2022年1月衡水市人民医院收治的160例子宫颈HSIL患者作为研究对象,均行宫颈锥切术治疗。根据是否存在锥切术后切缘阳性及病灶残留分为研究组(n=39)和对照组(n=121),术后切缘阳性或有病灶残留为研究组,术后切缘阴性为对照组。比较两组临床资料,采用Lasso-Logistic回归分析子宫颈HSIL患者锥切术后切缘阳性及病灶残留的影响因素。结果子宫颈HSIL患者锥切术后切缘阳性率为24.38%,切缘阳性患者中病灶残留率为48.72%。高危型人乳头瘤病毒(HR-HPV)、病变级别、累及象限、累及腺体、转化区类型、电切环形状、锥切深度、锥切宽度、锥切体积、接触性出血均为子宫颈HSIL锥切术后切缘阳性及病灶残留的独立影响因素(P<0.05)。结论HR-HPV、病变级别、累及象限、累及腺体、转化区类型、电切环形状、锥切深度、锥切宽度、锥切体积、接触性出血均为子宫颈HSIL患者锥切术后切缘阳性及病灶残留的影响因素,临床应结合相关影响因素优化治疗方案,以减少切缘阳性及病灶残留率。

关键词：子宫颈高度鳞状上皮内病变宫颈锥切术切缘阳性病灶残留

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CHARACTERIZING ULTRASONIC ECHO BY CWT AND ITS APPLICATION TO NONDESTRUCTIVE EVALUATION OF MATERIAL DAMAGES

CHARACTERIZING ULTRASONIC ECHO BY CWT AND ITS APPLICATION TO...

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2006 8th International Conference on Signal Processing

作者： MA Shiwei CHEN Guanghua LI Min liu zhongjie LIANG Weibin School of Metratronic ＆ Automation Shanghai Kay Lab of Power Station AutomationShanghai University

ISBN: (纸本)0780397371;9780780397378

＜正＞Ultrasonic echoes,which contain useful information related to material micro-damages in nondestructive evaluation, usually exhibit involved time-varying frequency *** this paper,Continuous wavelet transform（CWT） based time-frequency analysis for echo signal were discussed. Ultrasonic testing of pulse-echo technique with high frequency contact transducers was carried out to thermally degraded Cr-Mo steel samples processed by different high temperature heat treatment *** echo signals were analyzed by CWT based on Gabor *** major frequency dependent ultrasonic parameters,attenuation and velocity,were obtained from the local peak values at each frequency from the magnitude of ***,numerical correlations of the attenuation coefficients and group velocity with the sample’s internal grain size were *** method has a potential for quantitative evaluation to damage advance before crack initiation.

关键词： continuous wavelet transform time-frequency analysis ultrasonic echo nondestructive evaluation

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Global Ramp Uniformity Correction Method for Super-Large Array CMOS Image Sensors

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Chinese Journal of Electronics 2024年第2期33卷 415-422页

作者： Ruiming XU zhongjie GUO Suiyang liu Ningmei YU Xi'an University of Technology

Aiming at the problem of the non-uniformity of the ramp signal in the super-large array CMOS(complementary metal-oxide semiconductor) image sensors, a ramp uniformity correction method for CMOS image sensors is proposed in this paper. Based on the error storage technique, the ramp non-uniformity error is stored. And the input ramp signal of each column is shifted by level-shifting technique to eliminate the ramp non-uniformity *** on the 55 nm-1P4M CMOS process, this paper has completed the detailed circuit design and comprehensive simulation verification of the proposed method. Under the design conditions that the voltage range of the ramp signal is 1.4 V, the slope of the ramp signal is 71.908 V/ms, the number of pixels is 8192(H) × 8192(V), and a single pixel size is 10 μm, the correction method proposed in this paper reduces the ramp non-uniformity error from 7.89 m V to 36 μV. The differential non-linearity of the ramp signal is +0.0013/-0.004 LSB and the integral non-linearity is+0.045/-0.021 LSB. The ramp uniformity correction method proposed in this paper reduces the ramp non-uniformity error by 99.54% on the basis of ensuring the high linearity of the ramp signal, without significantly increasing the chip area and without introducing additional power consumption. The column fixed-pattern noise is reduced from 1.9%to 0.01%. It provides theoretical support for the design of high-precision CMOS image sensors.

关键词： Power demand Power transmission lines Array signal processing Noise Metals CMOS image sensors CMOS process

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Method of Single Event Effects Radiation Hardened Design for DC-DC Converter Based Load Transient Detection

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Chinese Journal of Electronics 2024年第5期33卷 1154-1164页

作者： zhongjie GUO Nan liu Hu LU Mengli LI Ziyi QIU School of Automation and Information Engineering Xi'an University of Technology

Aiming at the impact of load current change on single-event transient, the essential difference between single-event transient and load transient of DC-DC converter is deeply studied. A hardened circuit based on load transient detection is proposed. The circuit detects the load transient information in time and outputs a control signal to control the single event hardened circuit, thereby realizing the improvement of the transient characteristics of the system under dynamic conditions. Based on the 180 nm bipolar-CMOS-DMOS（BCD） process, the design and physical verification of a boost converter are completed. The experimental results show that the input voltage range is 2.9–4.5 V, the output voltage range is 5.8–7.9 V, and the load current is 0–55 mA. During load transients, the load detection circuit turns off the hardened circuit in time, avoiding system oscillation and widening the dynamic range of the hardening circuit. Under the single-event transient, the output voltage fluctuation of the system does not exceed the maximum ripple voltage, and the single-event transient suppression ability reaches more than 86%, the system can work well with linear energy transfer of about 100 Me V·cm2/mg.

关键词： Voltage fluctuations Fluctuations Radiation hardening (electronics) Energy exchange DC-DC power converters Dynamic range Transient analysis DC-DC converter Analog single-event transient effect Load transient detection Radiation hardened by design TMR

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Progress of additives for morphology control in organic photovoltaics

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Chinese Chemical Letters 2024年第6期35卷 38-46页

作者： zhongjie Li Xiangyue Kong Yuhao liu Huayu Qiu Lingling Zhan Shouchun Yin Key Laboratory of Organosilicon Chemistry and Materials Technology of Ministry of Education College of MaterialChemistry and Chemical EngineeringHangzhou Normal UniversityHangzhou 311121China

The microstructure of the active layer in organic photovoltaics(OPVs),such as the size of phase separation,purity of the phases,and molecular packing within each phase,plays a crucial role in influencing the behavior of excitons and charge carriers within the active *** is also a key determinant of the photovoltaic performance of the *** the optimization of OPV devices,the use of additives has been demonstrated to be an effective strategy in microstructure control,leading to enhanced ***,the quest for stable and efficient novel additives,along with an exploration and summarization of the mechanisms underlying additive-induced microstructure control,is essential for a better understanding of the developmental trends of high-performance *** this review,we categorize additives based on their chemical structures and discuss their effects on the microstructure of the active layer from both thermodynamic and kinetic ***,we elaborate on the working mechanisms and their impact on the photovoltaic performance of the *** review provides an overview of recent advances in additives for OPVs,offering potential guidance for the future development of additives and further optimization of the active layer in photovoltaic devices.

关键词： Organic photovoltaics Additives Active layer morphology Mechanisms Photovoltaic performances

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Single-cell transcriptomes provide insights into expansion of glial cells in Bombyx mori

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Insect Science 2024年第4期31卷 1041-1054页

作者： Xiaojing liu zhongjie Zhang Bo Hu Kai Chen Ye Yu Hui Xiang Anjiang Tan Jiangsu Key Laboratory of Sericultural Biology and Biotechnology School of BiotechnologyJiangsu University of Science and TechnologyZhenjiangJiangsu ProvinceChina Key Laboratory of Silkworm and Mulberry Genetic Improvement Ministry of Agriculture and Rural AffairsThe Sericultural Research InstituteChinese Academy of Agricultural SciencesZhenjiangJiangsu ProvinceChina Guangzhou Key Laboratory of Insect Development Regulation and Application Research Institute of Insect Science and Technology and School of Life SciencesSouth China Normal UniversityGuangzhouChina

The diversity of cell types in the brain and how these change during different developmental stages,remains largely *** life cycle of insects is short and goes through 4 distinct stages including embryonic,larval,pupal,and adult *** postembryonic life,the larval brain transforms into a mature adult version after *** silkworm,Bombyx mori,is a lepidopteran model ***,we characterized the brain cell repertoire of larval and adult *** by obtaining 50708 single-cell *** and 12 cell clusters from larval and adult brains were assigned based on marker genes,*** cell types include Kenyon cells,optic lobe cells,monoaminergic neurons,surface glia,and astrocyte *** further assessed the cell type compositions of larval and adult *** found that the transition from larva to adult resulted in great expansion of glial *** glial cell accounted for 49.8%of adult midbrain *** to flies and ants,the mushroom body kenyon cell is insufficient in ***,which accounts for 5.4%and 3.6%in larval and adult brains,*** of neuropeptide expression showed that the abundance and specificity of expression varied among individual ***,we found that ion transport peptide was specifically expressed in glial cells of larval and adult *** cell atlas dataset provides an important resource to explore cell diversity,neural circuits and genetic profiles.

关键词： brain neuropeptide silkworm single-cell transcriptome

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