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Dt1 inhibits SWEET-mediated sucrose transport to regulate photoperiod-dependent seed weight in soybean

Molecular Plant 2024年第3期17卷 496-508页

作者： Xiaoming Li zhonghui chen Haiyang Li Lin Yue Cuirong Tan Hongjie Liu Yilong Hu Yuhua Yang Xiani Yao Lingping Kong Xiang Huangi Bin Yu Chunyu Zhang Yuefeng Guan Baohui Liu Fanjiang Kong Xingliang Hou Guangdong Provincial Key Laboratory of Applied Botany&State Key Laboratory of Plant Diversity and Specialty Crops South China Botanical GardenChinese Academyof SciencesGuangzhou510650China Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design Guangzhou Key Laboratory of Crop Gene EditingInnovative Center of Molecular Genetics and EvolutionSchool of Life SciencesGuangzhou UniversityGuangzhou 510006China University of Chinese Academy of Sciences Beijing 100049China College of Agronomy and Biotechnology China Agricultural UniversityBeijing 100193China

Soybean is a photoperiod-sensitive short-day crop whose reproductive period and yield are markedly affected by day-length changes.Seed weight is one of the key traits determining the soybean yield;how-ever,the prominent genes that control the final seed weight of soybean and the mechanisms underlying the photoperiod's effect on this trait remain poorly understood.In this study,we identify SwW19 as a major locus controlling soybean seed weight by QTL mapping and determine Dt1,an orthologous gene of Arabidopsis TFL1 that is known to govern the soybean growth habit,as the causal gene of the SW19 locus.We showed that Dt1 is highly expressed in developing seeds and regulates photoperiod-dependent seed weight in soybean.Further analyses revealed that the Dt1 protein physically interacts with the sucrose transporter GmSWEET10a to negatively regulate the import of sucrose from seed coat to the embryo,thus modulating seed weight under long days.However,Dt1 does not function in seed development under short days due to its very low expression.Importantly,we discovered a novel natural allelic variant of Dt1(H4 haplotype)that decouples its pleiotropic effects on seed size and growth habit;i.e.,this variant remains functional in seed development but fails to regulate the stem growth habit of soybean.Collectively,our findings provide new insights into how soybean seed development responds to photoperiod at different latitudes,offering an ideal genetic component for improving soybean's yield by manipulating its seed weightandgrowth habit.

关键词： soybean seed weight photoperiod dt1 sucrose transport

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Design and development of a mitochondrial-targeted photosensitizer for two-photon fluorescence imaging and photodynamic therapy

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Journal of Materials Science & Technology 2023年第10期141卷 135-148页

作者： Yu chen Sheng Zhang Tianjiao cheng Wei Lin Linlin Mao zhonghui chen Yang Yang Hanqing Huang Jinqiu Li Zhiyong Ke Zhong-Kai Cui Institute of Cancer Affiliated Hospital of Putian UniversityPutian UniversityPutian 351100China Department of Cell Biology School of Basic Medical SciencesSouthern Medical UniversityGuangzhou 510515China Affiliated Putian Hospital of Southern Medical University Putian 351100China Guangdong Engineering Research Center for Translation of Medical 3D Printing Application Guangzhou 510515China

Mitochondria are well-acknowledged as ideal targets for tumor therapy due to their important role in energy supply and cellular signal regulation.Mitochondria-specific photosensitizers have been reported to be critical for inducing cell apoptosis.Two-photon fluorescence imaging provides a new technique for delineating biological structures and activities in deep tissues.Herein,we developed a new aggregation-induced emission(AIE)active photosensitizer by attaching a pyridinium group for mitochondrial target-ing.The rationally designed photosensitizer(TTTP)exhibited excellent photophysical properties,good biocompatibility,reactive oxygen species(ROS)stimulation ability,anticancer efficacy,and two-photon imaging properties.TTTP was highly taken up by cells and accumulated specifically in mitochondria but was selectively cytotoxic to cancer cells.Under light irradiation,the generation of ROS was significantly boosted,leading to actively induced apoptosis.The in vivo tumor photodynamic therapeutic efficacy of TTTP showed significant inhibition of tumor growth.Furthermore,the underlying mechanism of TTTP tu-mor suppression revealed that the apoptosis agonist Bax was markedly up-regulated while the antagonist Bcl-xL was down-regulated.This research provides a potential mitochondrial-targeted phototherapeutic agent for effective therapy and two-photon fluorescence imaging.

关键词： Mitochondrial-targeting Two-photon fluorescence imaging Photodynamic therapy Apoptosis Reactive oxygen species(ROS)

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Regulating electronic structure of two-dimensional porous Ni/Ni_(3)N nanosheets architecture by Co atomic incorporation boosts alkaline water splitting

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InfoMat 2022年第6期4卷 141-152页

作者： Kaixi Wang Yingying Guo zhonghui chen Donghai Wu Shouren Zhang Baocheng Yang Jianan Zhang Henan Provincial Key Laboratory of Nanocomposites and Applications Institute of Nanostructured Functional MaterialsHuanghe Science and Technology CollegeZhengzhouChina Key Lab for Special Functional Materials of Ministry of Education National&Local Joint Engineering Research Center for High-efficiency Display and Lighting TechnologySchool of Materials Science and EngineeringHenan UniversityKaifengChina College of Materials Science and Engineering Zhengzhou UniversityZhengzhouChina

Interfacial engineering is a powerful method to improve the bifunctional electrocatalytic performance of pure phase catalysts.While it is expected to further optimize the electronic configuration of heterojunctions to boost the reaction kinetics in hydrogen/oxygen evolution reaction(HER/OER),but remains a challenge.Herein,a novel in situ hybrid heterojunction strategy is developed to construct 2D porous Co-doped Ni/Ni_(3)N heterostructure nanosheets(Co-Ni/Ni_(3)N)by pyrolysis of partially cobalt substituted nickel-zeolitic imidazolate framework(CoNi-ZIF)nanosheets under NH3 atmosphere.A combined experimental and theoretical studies manifest that the hybrid heterostructures can display regulative electronic states and downshift d-band center from the Fermi level,as well as optimize the adsorption energy of reaction intermediates,thus reducing the thermodynamic energy barriers and accelerating the catalytic kinetics.Consequently,benefitting from the optimized electronic configuration,hierarchical hollow nanosheets architecture,and abundant doped heterojunctions,the hybrid Co-Ni/Ni_(3)N heterostructure catalyst exhibits efficient catalytic activity for both HER(60 mV)and OER(322 mV)at 10 mA cm^(-2)in alkaline media,which is 105 and 47 mV lower than that of pure Ni_(3)N,respectively.The electrochemically active surface area of Co-Ni/Ni_(3)N is two times higher than that of Ni3N.Furthermore,the coupled practical water electrolyzer requires a low voltage of 1.575 V to reach 10 mA cm^(-2),and it can be driven by a 1.5 V battery.This work highlights the interface engineering guidance for the rational establishment of hybrid interfaces by electronic modulation of interfacial effect for alkaline water splitting.

关键词： 2D porous nanosheet hybrid heterojunction interfacial engineering nickel nitride overall water splitting

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Two-dimensional polymer nanosheets for efficient energy storage and conversion

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Nano Research 2021年第6期14卷 2023-2036页

作者： Yumei Ren chengbing Yu zhonghui chen Yuxi Xu School of Materials Science and Engineering Shanghai UniversityShanghai201800China School of Materials Science and Engineering Zhengzhou University of AeronauticsZhengzhou450046China School of Engineering Westlake UniversityHangzhou310024China Key Lab for Special Functional Materials of Ministry of Education School of Materials Science and EngineeringHenan UniversityKaifeng475004China

As a promising graphene analogue,two-dimensional(2D)polymer nanosheets with unique 2D features,diversified topological structures and as well as tunable electronic properties,have received extensive attention in recent years.Here in this review,we summarized the recent research progress in the preparation methods of 2D polymer nanosheets,mainly including interfacial polymerization and solution polymerization.We also discussed the recent research advancements of 2D polymer nanosheets in the fields of energy storage and conversion applications,such as batteries,supercapacitors,electrocatalysis and photocatalysis.Finally,on the basis of their current development,we put forward the existing challenges and some personal perspectives.

关键词： two-dimensional(2D)polymer nanosheets 2D polymerization batteries supercapacitors photo(electro)catalysis

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Deformation and failure characteristics of sandstone under uniaxial compression using distributed fiber optic strain sensing

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Journal of Rock Mechanics and Geotechnical Engineering 2020年第5期12卷 1046-1055页

作者： Lingfan Zhang Duoxing Yang zhonghui chen Aichun Liu Institute of Crustal Dynamics China Earthquake AdministrationBeijing100085China College of Mechanics and Civil Engineering China University of Mining and TechnologyBeijing100083China

This paper investigates the deformation and fracture propagation of sandstone specimen under uniaxial compression using the distributed fiber optic strain sensing(DFOSS)technology.It shows that the DFOSS-based circumferential strains are in agreement with the data monitored with the traditional strain gage.The DFOSS successfully scans the full-field view of axial and circumferential strains on the specimen surface.The spatiotemporal strain measurement based on DFOSS manifests crack closure and elastoplastic deformation,detects initialization of microcrack nucleation,and identifies strain localization within the specimen.The DFOSS well observes the effects of rock heterogeneity on rock deformation.The advantage of DFOSS-based strain acquisition includes the high spatiotemporal resolution of signals and the ability of full-surface strain scanning.The introduction to the DFOSS technology yields a better understanding of the rock damage process under uniaxial compression.

关键词： Distributed fiber optic strain sensing (DFOSS) Uniaxial compression Strain localization

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粒径计算方法对细观混凝土数值建模的影响

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建筑材料学报 2024年第2期27卷 106-113,145页

作者：朱天宇陈忠辉张令非年庚乾中国矿业大学(北京)力学与建筑工程学院北京100083

采用最小包围盒(MBB)法确定骨料粒径,并与采用等体积球(EVS)法计算的骨料粒径进行比较,分析了这2种粒径计算方法对细观混凝土内部结构的影响.结果表明:采用MBB法计算的骨料粒径与室内试验采用筛分法确定的骨料粒径更为接近;与MBB法相比,... 详细信息

采用最小包围盒(MBB)法确定骨料粒径,并与采用等体积球(EVS)法计算的骨料粒径进行比较,分析了这2种粒径计算方法对细观混凝土内部结构的影响.结果表明:采用MBB法计算的骨料粒径与室内试验采用筛分法确定的骨料粒径更为接近;与MBB法相比,EVS法低估了球状、片状和盘状骨料的粒径,且被低估的骨料占比由大到小依次为球状、片状和盘状,EVS法高估了棒状骨料的粒径;粒径计算方法对细观混凝土内部结构的影响因骨料形状类型不同而呈现一定差异性.

关键词：混凝土粒径计算方法细观结构形状类型骨料粒径

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High mobility channel estimation method based on improved basis expansion model

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Digital Communications and Networks 2019年第2期5卷 76-83页

作者： Jinjin Huang Yisheng Zhao Zhixiang Dong Mengjia chen zhonghui chen College of Physics and Information Engineering Fuzhou UniversityFuzhou 350108China

In this paper, the problem of high mobility channel estimation in the Long-Term Evolution for Railway (LTE-R) communication system is investigated. By using a Basis Expansion Model (BEM), the channel impulse response is modeled as the sum of several basis functions multiplied by coefficients. By estimating the basis function coefficients, the fast time-varying channel can be approximated. In order to reduce the estimation error resulting from the high frequency basis function, the Generalized Complex Exponential BEM (GCE-BEM) is modified to form an Improved GCE-BEM (IGCE-BEM) by adding a correction coefficient to the basis function. Moreover, an Improved Baseline Tilting (IBT) method is proposed to reduce the Gibbs effect. In addition, linear interpolation, Gauss interpolation, and three-order Hermite interpolation are adopted to obtain the channel impulse response at non pilot locations based on the channel estimation results at pilot positions. The simulation results show that the IGCE-BEM outperforms the CE-BEM and GCE-BEM in terms of the Normalized Mean Squared Error (NMSE). The IB T method is better than the BT method in reducing the Gibbs effect. In addition, combined with the IBT, the IGCE-BEM also has low NMSE under high moving speed and high noise power. The performance of the threeorder Hermite interpolation method is higher than that of the linear interpolation and Gauss interpolation approaches.

关键词： Channel estimation LTE-R Basis expansion model Interpolation algorithm

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Basis expansion model for channel estimation in LTE-R communication system

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Digital Communications and Networks 2016年第2期2卷 92-96页

作者： Ling Deng zhonghui chen Yisheng Zhao College of Physics and Information Engineering Fuzhou University No. 2 Xueyuan Road Fuzhou 350108 Fujian China

This paper investigates fast time-varying channel estimation in LTE-R communication systems. The Basis Expansion Model (BEM) is adopted to fit the fast time-varying channel in a high-speed railway communication scenario. The channel impulse response is modeled as the sum of basis functions multiplied by different coefficients. The optimal coefficients are obtained by theoretical analysis. Simulation results show that a Generalized Complex-Exponential BEM (GCE-BEM) outperforms a Complex-Exponential BEM (CE-BEM) and a polynomial BEM in terms of Mean Squared Error (MSE). Besides, the MSE of the CE-BEM decreases gradually as the number of basis functions increases. The GCE-BEM has a satisfactory performance with the serious fading channel.

关键词： Channel estimation Basis expansion model LTE-R

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深部煤层气井压裂工艺研究及应用

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石化技术 2024年第6期31卷 257-259页

作者：陈忠辉华东油气分公司临汾煤层气分公司山西临汾041000

深部煤层气储量庞大、分布较广,然而煤层毛细裂缝联通性差、渗透性差,以至于传统开采方法的应用存在局限性,所以作为增产手段的煤层气井压裂工艺变成了关注的焦点。以文献研究法为主,结合实践体会与认知,重点对深部煤层气井压裂工艺展... 详细信息

深部煤层气储量庞大、分布较广,然而煤层毛细裂缝联通性差、渗透性差,以至于传统开采方法的应用存在局限性,所以作为增产手段的煤层气井压裂工艺变成了关注的焦点。以文献研究法为主,结合实践体会与认知,重点对深部煤层气井压裂工艺展开探索,并将相关工艺要点给予简要总结,力求能使我国深部煤层气资源得到合理开发和利用。

关键词：深部煤层煤层气井压裂工艺

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超高效液相色谱-串联质谱法测定饮料中高氯酸盐和氯酸盐

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卫生研究 2024年第1期53卷 102-108页

作者：陈东李倩刘平陈忠辉谢昀许秀丽张晶张峰北京市疾病预防控制中心食物中毒诊断溯源技术北京市重点实验室北京100013 北京市大兴区疾病预防控制中心北京102600 中国检验检疫科学研究院北京100176

目的建立同位素内标法定量测定饮料中高氯酸盐和氯酸盐的高效液相色谱-串联质谱法。方法液体饮料样品经甲醇溶液提取,固体饮料样品经乙酸水提取,PSA/C18基质分散固相萃取净化,再经Acquity CSH氟苯基色谱柱(100 mm×2.1 mm,1.7μm)分离,... 详细信息

目的建立同位素内标法定量测定饮料中高氯酸盐和氯酸盐的高效液相色谱-串联质谱法。方法液体饮料样品经甲醇溶液提取,固体饮料样品经乙酸水提取,PSA/C18基质分散固相萃取净化,再经Acquity CSH氟苯基色谱柱(100 mm×2.1 mm,1.7μm)分离,串联质谱法检测,内标法定量。结果高氯酸盐和氯酸盐在各自的线性范围内峰面积比与其质量浓度线性关系良好,相关系数(r)大于0.999;高氯酸盐和氯酸盐的检出限分别为0.2和1μg/L,定量限为0.5和3μg/L;两种化合物的平均回收率分别为84.0%~105.0%和82.7%~112.1%,相对标准偏差为4.2%~17.0%和5.5%~18.4%(n=6)。11类饮料样品中高氯酸盐含量为0.53~4.12μg/L,氯酸盐含量为3.27~61.86μg/L。结论本方法前处理简单、灵敏度高、准确、可靠,适用于饮料中高氯酸盐和氯酸盐的测定。

关键词：高氯酸盐氯酸盐同位素内标法饮料超高效液相色谱-串联质谱法

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