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Analysis of cytochrome P450 genes in silkworm genome (Bombyx mori)

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Science China(Life Sciences) 2005年第4期48卷 414-418页

作者： LI Bin XIA Qingyou LU Cheng ZHOU Zeyang xiang zhonghuai Key Sericultural Laboratory of Agricultural Ministry College of Sericulture&BiotechnologySouthwest Agricultural UniversityChongqing 400716China

We have searched the Bombyx mori genome for members of the major enzyme family,the Cytochrome P450s,which carry out multiple reactions to enable organisms to rid themselves of foreign *** a result,86 putative P450s were discovered in silkworm genome,which are thought to belong to 32 subfamilies.A comparative genomic analysis with Drosophila melanogaster reveals that the two insects have some similar P450 distribution pat-terns but still have some obvious ***,the diverse distribution exists in 7 p450 subfamilies,which are CYP4A,CYP4C,CYP4D,CYP6A,CYP6AE,CYP6B and ***-thermore,we collected expression sequence tag(EST)evidence for 49 putative P450s genes,which are expressed at the transcriptional level and more likely to be true P450s.

关键词： cytochrome P450 silkworm genome.

Phylogeny and evolutionary history of the silkworm

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Science China(Life Sciences) 2012年第6期55卷 483-496页

作者： SUN Wei YU HongSong SHEN YiHong BANNO Yutaka xiang zhonghuai ZHANG Ze Institute of Sericulture and Systems Biology Southwest UniversityChongqing 400715China Institute of Genetic Resources Graduate School of Bioresource and Bioenvironmental SciencesKyushu University Fukuoka 812-8581Japan Institute of Agricultural and Life Sciences Chongqing UniversityChongqing 400044China

The silkworm,Bombyx mori,played an important role in the old Silk Road that connected ancient Asia and ***,to date,there have been few studies of the origins and domestication of this species using molecular *** this study,DNA sequences of mitochondrial and nuclear loci were used to infer the phylogeny and evolutionary history of the domesticated silkworm and its *** of the phylogenetic analyses indicated a close relationship between the domesticated silkworm and the Chinese wild *** was estimated to have occurred about 4100 years ago(ya),and the radiation of the different geographic strains of *** about 2000 *** Chinese wild silkworm and the Japanese wild silkworm split about 23600 *** estimates are in good agreement with the fossil evidence and historical *** addition,we show that the domesticated silkworm experienced a population expansion around 1000 *** divergence times and the population dynamics of silkworms presented in this study will be useful for studies of lepidopteran phylogenetics,in the genetic analysis of domestic animals,and for understanding the spread of human civilizations.

关键词： silkworm domestication divergence time population dynamic population expansion

Genome-wide identification and expression analyses of cytochrome P450 genes in mulberry(Morus notabilis)

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Journal of Integrative Plant Biology 2014年第9期56卷 887-901页

作者： Bi Ma Yiwei Luo Ling Jia Xiwu Qi Qiwei Zeng zhonghuai xiang Ningjia He State Key Laboratory of Silkworm Genome Biology Southwest University

Cytochrome P450 S play critical roles in the biosynthesis of physiologically important compounds in *** compounds often act as defense toxins to prevent *** the present study,a total of 174 P450 genes of mulberry（Morus notabilis ***） were identified based on bioinformatics *** mulberry P450 genes were divided into nine clans and 47 families and were found to be expressed in a tissue-preferential *** genes were compared to the P450 genes in Arabidopsis *** CYP80,CYP92,CYP728,CYP733,CYP736,and CYP749 were found to exist in mulberry,and they may play important roles in the biosynthesis of mulberry secondary *** of the functional and metabolic pathways of these genes indicated that mulberry P450 genes may participate in the metabolism of lipids,other secondary metabolites,xenobiotics,amino acids,cofactors,vitamins,terpenoids,and *** results provide a foundation for understanding of the structures and biological functions of mulberry P450 genes.

关键词： Cytochrome P450 metabolism Morus notabilis pathway silkworm

An efficient and safe strategy for germ cell-specific automatic excision of foreign DNA in F1 hybrid transgenic silkworms

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Insect Science 2024年第1期31卷 28-46页

作者： Dingpei Long Rongpeng Liu Yang Huang Anyao Fu Yuli Zhang Zhanzhang Hao Qiang Li Hanfu Xu zhonghuai xiang Aichun Zhao State Key Laboratory of Resource Insects Institute of Sericulture and Systems BiologySouthwest UniversityChongqingChina Department of Biology Georgia State UniversityAtlantaGeorgiaUSA Guangxi Institute of Sericulture Science NanningGuangxiChina Department of Mathematics and Statistics Georgia State UniversityAtlantaGeorgiaUSA

The safety of transgenic technology is a major obstacle in the popularization and use of transgenic silkworms and their *** sericulture,only the first filial generation(F1)hybrid eggs produced by cross-breeding Japanese and Chinese original strains are usually used for the large-scale breeding of silkworms,but this may result in uncontrolled transgene dispersal during the popularization and application of the F1 hybrid transgenic *** address this issue,we developed a safe and efficient strategy using the GAL4/Upstream activating sequence(UAS)system,the FLP/flippase recognition target(FRT)system,and the gonad-specific expression gene promoters(RSHP1p and Nanosp)for the germ cell-specific automatic excision of foreign DNA in the F1 hybrid transgenic *** established 2 types of activator strains,R1p::GAL4-Gr and Nsp::GAL4-Gr,containing the testis-specific GAL4 gene expression cassettes driven by RSHP1p or Nanosp,respectively,and 1 type of effector strain,UAS::FLP-Rg,containing the UAS-linked FLP gene expression *** FLP recombinase-mediated sperm-specific complete excision of FRT-flanked target DNA in the F1 double-transgenic silkworms resulting from the hybridization of R1p::GAL4-Gr and UAS::FLP-Rg was 100%,whereas the complete excision efficiency resulting from the hybridization of Nsp::GAL4-Gr and UAS::FLP-Rg ranged from 13.73%to 80.3%.Additionally,we identified a gene,sw11114,that is expressed in both testis and ovary of Bombyx mori,and can be used to establish novel gonad-specific expression systems in transgenic *** strategy has the potential to fundamentally solve the safety issue in the production of F1 transgenic silkworm eggs and provides an important reference for the safety of transgenic technology in other insect species.

关键词： Bombyx mori FLP/FRT system GAL4/UAS system germ cell-specific excision safety of transgenic technology transgenic silkworm

QTL mapping of economically important traits in Silkworm (Bombyx mori)

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Science China(Life Sciences) 2004年第5期47卷 477-484页

作者： LU Cheng, LI Bin, ZHAO Aichun & xiang zhonghuai The Key Sericulture Laboratory of the Ministry of Agriculture of China, College of Sericulture & Biotechnology, Southwest Agricultural University, Chongqing 400716, China The Key Sericulture Laboratory of the Ministry of Agriculture of China College of Sericulture & Biotechnology Southwest Agricultural University Chongqing China

A backcrossed population (BC1) was derived from a cross between C100 and Dazao. AFLP technique was employed for mapping the QTLs. The QTLs for the whole cocoon weight, cocoon shell weight, ratio of cocoon shell, weight of pupae etc. were analyzed and 11 QTLs were detected based on the constructed linkage map. Two QTLs for whole cocoon weight were local-ized on linkage group 6 and 19; three QTLs for cocoon shell weight were localized on linkage group 3, 14 and 19; three QTLs for ratio of cocoon shell were localized on the linkage group 2, 11 and 15, and three QTLs for the weight of pupae were localized on linkage 2, 14 and 19. All these have laid an important base for the marker assisted breeding of the silkworm.

关键词： Bombyx mori,genetic linkage map,AFLP,QTL,green cocoon.

Chromosome-level Genomes Reveal the Genetic Basis of Descending Dysploidy and Sex Determination in Morus Plants

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Genomics, Proteomics & Bioinformatics 2022年第6期20卷 1119-1137页

作者： Zhongqiang Xia Xuelei Dai Wei Fan Changying Liu Meirong Zhang Peipei Bian Yuping Zhou Liang Li Baozhong Zhu Shuman Liu Zhengang Li Xiling Wang Maode Yu zhonghuai xiang Yu Jiang Aichun Zhao State Key Laboratory of Silkworm Genome Biology Institute of Sericulture and Systems BiologySouthwest UniversityChongqing 400716China Key Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100China Key Laboratory of Coarse Cereal Processing Ministry of Agriculture and Rural AffairsChengdu UniversityChengdu 610106China The Sericultural and Apicultural Research Institute Yunnan Academy of Agricultural SciencesMengzi 661100China College of Sericulture Textile and Biomass SciencesSouthwest UniversityChongqing 400716China

Multiple plant lineages have independently evolved sex chromosomes and variable karyotypes to maintain their sessile lifestyles through constant biological *** notabilis,a dioecious mulberry species,has the fewest chromosomes among Morus spp.,but the genetic basis of sex determination and karyotype evolution in this species has not been *** this study,three high-quality genome assemblies were generated for Morus spp.[including dioecious ***(male and female)and Morus yunnanensis(female)]with genome sizes of 301–329 Mb and were grouped into six *** a combination of genomic approaches,we found that the putative ancestral karyotype of Morus species was close to 14 protochromosomes,and that several chromosome fusion events resulted in descending dysploidy(2n=2x=12).We also characterized a~6.2-Mb sex-determining region on chromosome *** potential male-specific genes,a partially duplicated DNA helicase gene(named MSDH)and three Ty3_Gypsy long terminal repeat retrotransposons(named MSTG1/2/3),were identified in the Y-linked area and considered to be strong candidate genes for sex determination or *** genomic analysis showed that Guangdong accessions in China were genetically similar to Japanese accessions of *** addition,genomic areas containing selective sweeps that distinguish domesticated mulberry from wild populations in terms of flowering and disease resistance were *** study provides an important genetic resource for sex identification research and molecular breeding in mulberry.

关键词： Mulberry Karyotype evolution Dioecy Sex determination Population genomics

A silkworm hemolymph protein is a prophenoloxidase activation blocker

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American Journal of Molecular Biology 2012年第4期2卷 332-340页

作者： Bilang Liu Xiwu Qi Qi Han Ling Jia zhonghuai xiang Ningjia He State Key Laboratory of Silkworm Genome Biology Southwest University Chongqing China

Melanization in insect hemolymph is triggered by the recognition of pathogen-associated molecular patterns via pattern recognition receptors. The signal transduction leads to the activation of the prophenoloxidase and hence the generation of melanin. The proPO activation process must be tightly controlled to minimize the host damage caused by reactive intermediates during melanin synthesis. The full-length cDNA sequence of a 20 kDa hemolymph protein from Bombyx (Bmhp20) was determined. Bmhp20 gene was expressed in larval fat body, integument, trachea, and ovary and was induced by the challenge of B. bombyseptieus. Binding of recombinant Bmhp20 to microbial cell wall components as well as gram-positive bacteria and fungi was confirmed. Phenoloxidase activity assay indicated that recombinant Bmhp20 blocked the proPO activation in hemolymph that was triggered by peptidoglycan or beta-1, 3-glucan. Our data suggest that Bmhp20 plays bifunctional roles in silkworm humoral responses: to participate in pattern recognition and to block the activation of proPO.

关键词： Bombyx Mori Humoral Responses Pattern Recognition Receptors Phenoloxidase Activity

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我国古代“浴蚕”的功能文化学阐释

蚕业科学 2018年第1期44卷 143-148页

作者：李发向仲怀西南大学汉语言文献研究所重庆400715 家蚕基因组生物学国家重点实验室重庆400715

“浴蚕”实为提高蚕种（卵）质量的一项技术措施,但在古人看来,“浴蚕”首先是一种神圣的信仰仪式,渗透了原始图腾崇拜的意义,有着系统的仪式规程。“浴蚕”发展到北魏以后,有了科学意识的萌芽,其行为在客观上促进了蚕的胚胎发育,以及... 详细信息

“浴蚕”实为提高蚕种（卵）质量的一项技术措施,但在古人看来,“浴蚕”首先是一种神圣的信仰仪式,渗透了原始图腾崇拜的意义,有着系统的仪式规程。“浴蚕”发展到北魏以后,有了科学意识的萌芽,其行为在客观上促进了蚕的胚胎发育,以及起到了对卵面清洁消毒和剔除不良卵的作用等。对“浴蚕”史料的发掘可以看出,“浴蚕”行为从周代到清代发生了很大的变化,反映出古人在选留优良蚕种（卵）方面积累了丰富的实践经验。虽然用现代蚕业科技的眼光看古人的“浴蚕”行为存在许多不足,但它依然是先辈遗留的一笔宝贵文化财富。

关键词：浴蚕图腾崇拜仪式规程胚胎发育清洁消毒

“一带一路”话语传播与“丝绸之路”研究热点

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蚕业科学 2018年第4期44卷 509-515页

作者：陶红向仲怀西南大学新闻传媒学院重庆400715 家蚕基因组生物学国家重点实验室重庆400715

＂一带一路＂成为中国式话语现象,是基于我国经济实力提升及与＂丝绸之路＂的历史渊源关系,＂一带一路＂话语传播将对＂丝绸之路＂学术研究产生重要影响。伴随互联网时代获得探讨学术命题资料的便捷,西方学者提出＂丝绸之路＂的价值在... 详细信息

＂一带一路＂成为中国式话语现象,是基于我国经济实力提升及与＂丝绸之路＂的历史渊源关系,＂一带一路＂话语传播将对＂丝绸之路＂学术研究产生重要影响。伴随互联网时代获得探讨学术命题资料的便捷,西方学者提出＂丝绸之路＂的价值在于它是东西方宗教、艺术、语言和新技术交流的大动脉,中国学者深入研究＂丝绸之路＂对我国诗歌、舞蹈、乐器、蜀锦花纹、剪纸、佛教播迁、域外农作物引进、＂南京布＂贸易等的深刻影响,认为＂丝绸之路＂是中西文明交融的重要历史舞台,且＂一带一路＂倡议与＂丝绸之路＂的发展变迁有密切关系。

关键词：一带一路话语传播丝绸之路中西文明历史价值