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Cold Stress-induced Glucosyltransferase CsUGT78A15 is Involved in the Formation of Eugenol Glucoside in Camellia sinensis

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Horticultural Plant Journal 2020年第6期6卷 439-449页

作者： Mingyue Zhao Binbin Cai Jieyang Jin Na Zhang Tingting Jing Jingming Wang Yuting Pan Zixiang Zhou Yifan Zhao Yingying Feng Feng Yu Mengting Zhang Yating Li zhonghua liu Chuankui Song State Key Laboratory of Tea Plant Biology and Utilization International Joint Laboratory on Tea Chemistry and Health EffectsAnhui Agricultural UniversityHefei 230036China Key Laboratory of Tea Science of Ministry of Education National Research Center of Engineering Technology for Utilization of Functional Ingredients from BotanicalsCollege of HorticultureHunan Agricultural UniversityChangsha 410128China

Eugenol is a natural phenolic compound known for its health-promoting properties and its ability to add a floral scent to tea *** eugenol glycosides have been identified and shown to make important contributions to fruit floral ***,the details of their biosynthesis and metabolism in tea plants are still ***,eugenol glucoside was unambiguously identified as a native metabolite in the tea plant,and its biosynthesis was shown to be induced by low temperature *** the analysis of UGTs induced by low temperature,the glycosyltransferase CsUGT78A15 was identified in tea,and its encoded protein was shown to catalyze the glucosylation of ***/Km ratios showed that eugenol was the most suitable substrate for *** donor preference analysis showed that CsUGT78A15 had a higher selectivity for glucose,followed by galactose and glucuronic *** expression of CsUGT78A15was correlatedwith the accumulation of eugenol glucoside in different tissues and genotypes of ***-regulation of CsUGT78A15 led to a decreased eugenol glucoside content under cold stress,indicating that CsUGT78A15 plays an important role in the biosynthesis of eugenol glucoside under cold *** identification of eugenol glucoside in the tea plant and the discovery of a cold stress-induced eugenol glucosyltransferase in tea provide the foundation for the improvement of tea flavor under cold stress and the biotechnological production of eugenol glucoside.

关键词： Camellia sinensis Eugenol Glucosyltransferase Cold stress

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运动员语音音强特征诊断运动疲劳的探索性研究

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天津体育学院学报 2009年第5期24卷 446-448页

作者：刘仲华西安体育学院社会体育系西安710068

提高大运动负荷的训练效率需要准确的运动疲劳状态判断,语音音强特征能够表现运动员的疲劳信息,通过语音信号处理、神经网络建模、灰色关联分析等手段,表明语音音强特征作为运动疲劳判断手段具有可行性。建立了运动疲劳神经网络诊断模型... 详细信息

提高大运动负荷的训练效率需要准确的运动疲劳状态判断,语音音强特征能够表现运动员的疲劳信息,通过语音信号处理、神经网络建模、灰色关联分析等手段,表明语音音强特征作为运动疲劳判断手段具有可行性。建立了运动疲劳神经网络诊断模型,对语音音强数据进行学习和归纳,确定了语音音强特征与运动疲劳状态之间的模式。模型诊断与定性判断的比较结果表明:对于运动疲劳状态判断,音强特征具有较好的判断等级区分度。建议教练员把直觉判断与音强特征结合起来,可以提高对运动疲劳状态判断的效果。

关键词：运动疲劳诊断语音音强灰色关联分析神经网络

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激光熔覆原位合成TiC-Cr_7C_3-Ti-Ni金属复合材料涂层

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中国激光 2008年第7期35卷 1091-1094页

作者：张维平刘中华大连理工大学材料科学与工程学院辽宁大连116024

利用激光熔覆工艺在Ti-6Al-4V合金表面制备出原位自生TiC-Cr7C3-Ti-Ni金属复合陶瓷涂层,以改善材料表面的综合性能。采用X射线衍射(XRD)、扫描电镜(SEM)、能量分散仪(EDS)、电子探针显微分析仪(EPMA)等手段对复合涂层微观组织结构进行... 详细信息

利用激光熔覆工艺在Ti-6Al-4V合金表面制备出原位自生TiC-Cr7C3-Ti-Ni金属复合陶瓷涂层,以改善材料表面的综合性能。采用X射线衍射(XRD)、扫描电镜(SEM)、能量分散仪(EDS)、电子探针显微分析仪(EPMA)等手段对复合涂层微观组织结构进行研究。结果表明,复合涂层主要由-βTi,-γNi固溶体及分布于晶间的Ti/TiC或Ti/Cr7C3共晶组成;晶内为贫Ni,C的-βTi固溶体组织,晶间为富Ti,C的Ti/TiC和Ti/Cr7C3共晶组织;随着复合涂层成分的变化,激光熔覆原位合成物的量发生相应的变化,涂层熔区内晶体生长形态从网络状晶向树枝晶、等轴晶过渡;复合涂层显微硬度值较基体有显著提高。

关键词：激光技术激光熔覆涂层原位合成显微组织

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Characteristics of the Variation of Cross Fault Short-Baseline and Short-Range Leveling in Western Yunnan Earthquake Test Site Before Lijiang M_S7.0 Earthquake

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Earthquake Research in China 1998年第4期12卷 46-56页

作者： Li zhonghua, Su Youjing, and liu ZhuyinSeismological Bureau of Yunnan Province, Kunming 650041, China

Results of analysis of variation of cross fault short-baseline and short-range leveling in Western Yunnan Earthquake Test Site (WYETS), results show that among five observation stations of cross fault short-baseline and short-range leveling in WYETS before the Lijiang MS7.0 Earthquake occurred in February 1996 only Yongsheng observation station (epicentral distance 82 km) located at Chenghai fault shows great variation about one year before the earthquake. And the nearest observation station, Lijiang (epicentral distance 42 km); presents great coseismic variation, but does not show obvious anomalous variation before the earthquake. There are no significant variations related to the earthquake at the other three observation stations. Two methods are used in analysis of the observed data and some valuable results have been obtained.

关键词： Cross fault measurement Lijiang MS7.0 earthquake Western Yunnan Earthquake Test Site.

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PLGA nanoparticle encapsulated paclitaxol for sustained release and its anticancer effect for HeLa cells in vitro

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生物物理学报 2009年第S1期25卷 298-299页

作者： Hong Yang, Kai Li, zhonghua liu, Zhenglong Ren, Yiyao liu , Department of Biophysics, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan 610054, P.R.China

Poly (D,L-lactide-co-glycolide) (PLGA) is a biodegradable and biocompatible polymer material for drug deliver system. The aim of this study is to synthesize drug-loaded

关键词： PLGA nanoparticle encapsulated paclitaxol for sustained release and its anticancer effect for HeLa cells in vitro

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Splitting of Degenerate Superatomic Molecular Orbitals Determined by Point Group Symmetry

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Chinese Physics Letters 2023年第11期40卷 1-5页

作者：王瑞李佳芮刘中华万晨曦王志刚 Institute of Atomic and Molecular Physics Jilin UniversityChangchun 130012China Key Laboratory of Material Simulation Methods&Software of Ministry of Education College of PhysicsJilin UniversityChangchun 130012China Institute of Theoretical Chemistry College of ChemistryJilin UniversityChangchun 130023China

We first confirm an idea obtained from first-principles calculations,which is in line with symmetry theory:Although superatomic molecular orbitals(SAMOs) can be classified according to their angular momentum similar to atomic orbitals,SAMOs with the same angular momentum split due to the point group symmetry of *** on this idea,we develop a method to quantitatively modulate the splitting spacing of molecular orbitals in a superatom by changing its structural symmetry or by altering geometric parameters with the same symmetry through expansion and compression ***,the modulation of the position crossover is achieved between the lowest unoccupied molecular orbital and the highest occupied molecular orbital originating from the splitting of different angular momenta,leading to an effective reduction in system *** phenomenon is in line with the implication of the Jahn–Teller *** work provides insights into understanding and regulating the electronic structures of superatoms.

关键词： effect symmetry angular

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中空波纹夹层钢管混凝土柱抗震性能拟静力试验研究

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东南大学学报（自然科学版） 2024年第3期54卷 539-548页

作者：舒赣平姚震张萌刘忠华东南大学土木工程学院南京211189 宝胜系统集成科技股份有限公司扬州225800

为了研究中空波纹夹层钢管混凝土柱的抗震性能,以轴压比、空心率、长细比和截面形式为试验参数,开展了中空波纹夹层钢管混凝土柱的拟静力试验,分析了试件的破坏模式、承载能力、延性与耗能能力、刚度退化和强度退化等性能指标.结果表明... 详细信息

为了研究中空波纹夹层钢管混凝土柱的抗震性能,以轴压比、空心率、长细比和截面形式为试验参数,开展了中空波纹夹层钢管混凝土柱的拟静力试验,分析了试件的破坏模式、承载能力、延性与耗能能力、刚度退化和强度退化等性能指标.结果表明:中空波纹夹层钢管混凝土柱的水平承载力较高,滞回曲线饱满,无明显捏缩现象,延性和耗能能力良好,同级循环荷载作用下性能稳定;试件的破坏模式为延性破坏,具体表现为柱底钢管鼓曲变形、混凝土压溃、局部缺陷处管壁撕裂;空心率较小的试件承载力较高,耗能能力较强;当长细比由20增至30时,试件位移延性系数降低了47.49%,承载力也明显下降;随着轴压比的增大,试件耗能能力逐渐降低.

关键词：中空波纹夹层钢管混凝土柱抗震性能拟静力试验延性耗能

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A designed chimeric Nav1.8 channel which can be functionally expressed in HEK293T cells

A designed chimeric Nav1.8 channel which can be functionally...

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第12届生物毒素研究及医药应用学术大会

作者： Cheng Tang Yunxiao Zhang Xi Zhou zhonghua liu Songping Liang College of Life Sciences Hunan Normal University

Among the nine VGSC subtypes,Nav1.7 and Nav1.8 are attractive therapeutic targets for ***,the heterologous expression of Nav1.8 in mammalian cell lines in past years has achieved limited *** this study,we designed and constructed a chimeric channel by replacing all the transmembrane segments of human Nav1.5 with those of rat Nav1.8 *** resulting hybrid channel was named as DCNav1.8 and was abundantly expressed in Hek293 t as tested by patch-clamp ***1.8 exhibited normal voltage gating properties with a Va and Vh of15.2±1.5 mV and-17.3±1.8 mV *** differences were observed when compared with those of DRG TTX-R Nav1.8 channel or native Nav1.8 channel expressed in ND7/23 *** importantly,the pharmacological properties of DCNav1.8 resembled those of native Nav1.8 by testing several known Nav1.8 antagonists（Lidocaine,APETx-2 and MrVIB）.Single site mutation of amino acids located in the domain Ⅱ extracellular loops of DCNav1.8 to the corresponding ones in Navl.9 did not changed the affinity of MrVIB to *** molecular basis for the preference of MrVIB to Nav1.8 might reside in the pore loop of channel’s domain Ⅲ.In summary,our study provided a reliable and convenient platform for Nav1.8 expression which could be used in full-auto patch clamp system for high-throughput Nav1.8 modifiers screening and in intensive study of the molecular mechanism of drug-Nav1.8 interaction.

关键词： Nav1.8 chimeric channel heterologous expression patch clamp

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航空发动机卡箍装配应力试验与装配参数控制方法

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航空动力学报 2020年第2期35卷 368-377页

作者：刘中华贾铎王鑫刘鑫高东武中国航空发动机集团有限公司沈阳发动机研究所沈阳110015

通过理论公式推导分析影响卡箍装配应力的主要参数,并通过试验方法对影响卡箍装配应力的因素进行了系统分析与研究,以3种规格、每种规格5个样本的卡箍作为试验对象,测试并统计分析螺栓拧紧力矩值、加载次数、装配方法对卡箍的应力影响... 详细信息

通过理论公式推导分析影响卡箍装配应力的主要参数,并通过试验方法对影响卡箍装配应力的因素进行了系统分析与研究,以3种规格、每种规格5个样本的卡箍作为试验对象,测试并统计分析螺栓拧紧力矩值、加载次数、装配方法对卡箍的应力影响规律。研究结果表明:靠近螺栓孔的卡箍表面为应力集中位置,卡箍随加载次数的增多应力呈降低趋势,但重复加载次数不宜过多,会导致卡箍局部较大的变形和磨损,增加2 mm垫片的方法对降低卡箍应力水平的效果最好,下降率可达46.8%;相比之下,限制卡箍位移后加载的方法应力下降率约为3.64%,而加载后校正的方法应力下降率约为2.86%。

关键词：卡箍拧紧力矩加载次数装配应力分散度控制方法

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Three tarantula toxins acting on Nav1.5 reveals the diversity of toxin-channel interaction

Three tarantula toxins acting on Nav1.5 reveals the diversit...

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第12届生物毒素研究及医药应用学术大会

作者： Xi Zhou Cheng Tang Yunxiao Zhang zhonghua liu Songping Liang College of Life Sciences Hunan Normal University

Tarantula toxins potently target voltage-gated sodium channels(Navs) through binding to voltage sensor domains,and they are powerful tools for the investigation of *** the present study,we isolated and characterized three peptide toxins named JZTX-36,-38 and-39 from venom of Chilobrachys *** toxins could modulate human Na1.5 currents with different effects on Na1.5 channel activation and inactivation ***-36 and JZTX-39 interacted with site 4 to inhibit the activation of Nav1.5 and shift the steady-state activation voltage in a depolarization direction,whereas JZTX-38,which differs to JZTX-39 with only one amino acid located at the extreme C-terminal of the peptide,bound to the site 3 and site 4 simultaneously and barely affected steady-state *** results not only further confirmed that the S3-S4 linkers of domain Ⅱ and domain Ⅳ are hot spots for toxin interaction,but also revealed functional diversification of tarantula ***,many toxins can target a same binding site and one toxin can have two or more binding sites in *** addition,our results also suggest that spider toxins might be ideal template for designing ion channel’s modulators and useful probes for investigating the mechanism of toxin-channel interaction.

关键词： Navl.5 tarantula toxins toxin-channel interaction

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