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Injectable and biofunctionalized fibrin hydrogels co-embedded with stem cells induce hair follicle genesis

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Regenerative Biomaterials 2023年第1期10卷 53-63页

作者： Haiyan Chen Xiaoxiao Ma Mengqi Zhang zhonghua liu The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development College of Life SciencesHunan Normal UniversityChangsha 410081People’s Republic of China Tsinghua Shenzhen International Graduate School Tsinghua UniversityShenzhen 518055People’s Republic of China

Fibrin-based hydrogels have been widely used in various tissue engineering because of their biocompatibility,biodegradability,tunable mechanical characteristics and nanofibrous structural ***,their ability to support stem cells for hair follicle neogenesis is *** this study,we investigated the effect of fibrin hydrogels in supporting skin-derived precursors(SKPs)in hair follicle *** results showed that SKPs in fibrin hydrogels with high cell viability and proliferation,the stemness of SKPs could be maintained,and the expression of hair induction signature genes such as akp2 and nestin was ***,hair follicle reconstruction experiments showed de novo hair genesis in mice and the hairs persisted for a long time without teratoma *** importantly,the blood vessels and sebaceous glands were also *** study demonstrated that fibrin hydrogels are promising in hair follicle regeneration and have potential application in clinical settings for alopecia and wound healing.

关键词： fibrin hydrogels fibrinogen hair follicle neogenesis alopecia skin-derived precursors

Optimization of aero-engine pipeline for avoiding vibration based on length adjustment of straight-line segment

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Frontiers of Mechanical Engineering 2022年第1期17卷 100-116页

作者： Wenhao JI Wei SUN Donghai WANG zhonghua liu School of Mechanical Engineering and Automation Northeastern UniversityShenyang 110819China Key Laboratory of Vibration and Control of Aero-Propulsion Systems Department of Education of China Northeastern UniversityShenyang 110819China Shenyang Area 2nd Military Representative Room of Air Force Equipment Department Shenyang 110043China

In the design and troubleshooting of aero-engine pipeline,the vibration reduction of the pipeline system is often achieved by adjusting the hoop layout,provided that the shape of pipeline remains ***,in reality,the pipeline system with the best antivibration performance may be obtained only by adjusting the pipeline *** this paper,a typical spatial pipeline is taken as the research object,the length of straight-line segment is taken as the design variable,and an innovative optimization method of avoiding vibration of aero-engine pipeline is *** relationship between straight-line segment length and parameters that determine the geometric characteristics of the pipeline,such as the position of key reference points,bending angle,and hoop position,are derived in *** on this,the parametric finite element model of the pipeline system is *** the maximum first-order natural frequency of pipeline as the optimization objective and introducing process constraints and vibration avoidance constraints,the optimization model of the pipeline system is *** genetic algorithm and the golden section algorithm are selected to solve the optimization model,and the relevant solution procedure is described in ***,two kinds of pipelines with different total lengths are selected to carry out a case *** on the analysis of the influence of straight-line segment length on the vibration characteristics of the pipeline system,the optimization methods developed in this paper are *** show that the developed optimization method can obtain the optimal single value or interval of the straight-line segment length while avoiding the excitation *** addition,the optimization efficiency of the golden section algorithm is remarkably higher than that of the genetic algorithm for length optimization of a single straight-line segment.

关键词： length adjustment spatial pipeline aero-engine vibration avoidance optimization genetic algorithm golden section algorithm

Cell therapy in diabetes: current progress and future prospects

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Science Bulletin 2015年第20期60卷 1744-1751页

作者： Ying Wang Tang Hai Lei liu zhonghua liu Qi Zhou College of Life Sciences Northeast Agricultural University of China State Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences

Diabetes mellitus, characterized by the impaired metabolism of insulin secretion in β cells, is becoming one of the most prevalent diseases around the world. Recently, cell replacement based on differentiation of various pluripotent stem cells, including embryonic stern cells, induced pluripo- tent stem cells and multipotent stem cells, such as bone mar- row mesenchymal stem cells, adipose-derived stem cells and gnotobiotic porcine skin-derived stem cells, is becoming a promising therapeutic strategy. Cells derived from pancreatic tissues or other tissues that are relevant to β cell differentiation have also been used as cell source. However, in spite of hopeful experimental results, cell therapy in diabetes still confronts certain obstacles, such as purity of cells, functional differentiation of stem cells and possible tumorigenesis, which, in turn, lead to the seeking of new-generation tools, such as xenogenetic materials. In this review, we will sum- marize the current knowledge and future prospects of cell therapy in diabetes mellitus.

关键词： Diabetes Cell therapy Signalingpathway Xenotransplantation

Peptide nanotube loaded with a STING agonist,c-di-GMP,enhance cancer immunotherapy against melanoma

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Nano Research 2023年第4期16卷 5206-5215页

作者： Ziyuan Zhang Juan liu Min Xiao Quanfeng Zhang zhonghua liu Meiyan liu Peng Zhang Youlin Zeng Key Laboratory of the Assembly and Application of Organic Functional Molecules of Hunan Province Hunan Normal UniversityChangsha 410081China Laboratory of Chemical Biology and Traditional Chinese Medicine Research(Ministry of Education of China) Hunan Normal UniversityChangsha 410081China The National&Local Joint Engineering Laboratory of Animal Peptide Drug Development College of Life SciencesHunan Normal UniversityChangsha 410081China

The activation of the stimulating factor of the interferon gene(STING)pathway can enhance the immune response within the *** diguanylate monophosphate(c-di-GMP)is a negatively charged,hydrophilic STING agonist,however,its effectiveness is limited due to the poor membrane permeability and low ***,we introduced KL-7 peptide derived from Aβamyloid fibrils that can self-assemble to form nanotubes to load and deliver c-di-GMP,which significantly enhanced c-di-GMP’s effectiveness and then exhibited a robust“in situ immunity”to kill melanoma ***-7 peptide nanotube,also called PNT,was loaded with negatively charged c-di-GMP via electrostatic interaction,which prepared a nanocomposite named *** of RAW 264.7 cells(leukemia cells in mouse macrophage)with c-di-GMP-PNT markedly stimulated the secretion of IL-6 and INF-βalong with phospho-STING(Ser365)protein expression,indicating the activation of the STING *** the unilateral flank B16-F10(murine melanoma cells)tumor-bearing mouse model,compared to PNT and cdi-GMP,c-di-GMP-PNT can promote the expression of INF-β,TNF-α,IL-6,and IL-1β.At the same time,up-regulated CD4 and CD8 active T cells kill tumors and enhance the immune response in tumor tissues,resulting in significant inhibition of tumor growth in tumor-bearing *** importantly,in a bilateral flank B16-F10 tumor model,both primary and distant tumor growth can also be significantly inhibited by ***,c-di-GMP-PNT demonstrated no obvious biological toxicity on the main organs(heart,liver,spleen,lung,and kidney)and biochemical indexes of *** summary,our study provides a strategy to overcome the barriers of free c-di-GMP in the tumor microenvironment and c-di-GMP-PNT may be an attractive nanomaterial for anti-tumor immunity.

关键词： cyclic diguanylate monophosphate stimulating factor of the interferon gene(STING) peptide nanotubes in situ immunity tumor immunotherapy

KEYHOLE IMAGE PROCESSING OF VARIABLE POLARITY PLASMA ARC WELDING BASED ON WAVELET TRANSFORM

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Chinese Journal of Mechanical Engineering 2001年第1期14卷 62-66+73页

作者： liu zhonghua Wang Qilong Zhu Jiaqi Welding Department, Harbin Institute of Technology 哈尔滨工业大学黑龙江哈尔滨

In order to realize the feedback control for variable polarity plasma arc welding (VPPAW) formation in the welding process, the geometrical sizes of the keyhole image must be extracted. With the properties of multiscale edge through the wavelet theory, the edge points were detected by getting the maximum modules of the gradient vector in the dircetion towards which the gradient vector points in the image plane. At coarse scales, the local maxima of modules have different positions and only detected the sharp edge. At fine scale, there are many maxima created by the image noise. The best scale where the edges are well discriminated from noises is discussed by the multiscale transform. At last, a new method of peak analysis for threshold selection is provided. It is based on the wavelet transform which provides a multiscale analysis of the information of the histogram. Many experiments show these ways are effective for the keyhole image to get the geometry parameters of the keyhole in the real time VPPAW image processing.

关键词： PAW Variable polarity Keyhole Image

Junshanyinzhen tea extract prevents obesity by regulating gut microbiota and metabolic endotoxemia in high-fat diet fed rats

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Food Science and Human Wellness 2024年第4期13卷 2036-2047页

作者： Jian Ouyang Xiuping Li Changwei liu Danmin Lu Jie Ouyang Fang Zhou Qi liu Jianan Huang zhonghua liu Key Laboratory of Tea Science of Ministry of Education Hunan Agricultural UniversityChangsha 410128China National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals Hunan Agricultural UniversityChangsha 410128China Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients Hunan Agricultural UniversityChangsha 410128China Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops Ministry of Agriculture and Rural AffairsHunan Agricultural UniversityChangsha 410128China

Obesity is associated with gut dysbiosis and metabolic *** tea extract(JSTE)reduced fat accumulation and body weight in obese ***,the effects and mechanism of JSTE in preventing obesity were ***,we used different doses of JSTE(75,150 and 300 mg/(kg·day))to evaluate the effect on high-fat diet(HFD)-induced rats under 8 weeks of ***,our results showed that JSTE could significantly reduce body weight gain,blood lipid levels and fat accumulation,improve fatty damage in liver tissue(P<0.05).In addition,JSTE increased the expression of intestinal tight junction proteins(P<0.05),relieved metabolic endotoxemia(P<0.05)and chronic low-grade inflammation in HFD *** of fecal samples showed that JSTE could effectively reverse the microbial diversity and the ratio of Firmicutes to Bacteroidetes to normal levels in HFD-fed *** and Erysipelotrichaceae,which are positively related to obesity,were decreased by JSTE intervention(P<0.05).while Bifidobacteriaceae,Bacteroidaceae,Akkermansia,and Clostridium,which are negatively related to obesity,were ***,these results suggested that JSTE might effectively prevent obesity by modulating gut microbiota dysbiosis,intestinal barrier dysfunction,metabolic endotoxemia and chronic low-grade infl ammation in HFD-induced rats.

关键词： Junshanyinzhen tea Obesity Gut microbiota Gut barrier function Metabolic endotoxemia

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The role of cholesterol in modifying the lipid-lowering effects of Fuzhuan brick-tea in Caenorhabditis elegans via SBP-1/SREBP

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Food Science and Human Wellness 2023年第6期12卷 2297-2305页

作者： Lianyun Pan Yan Lu Shen Dai Xiangyue Tang Ligui Xiong zhonghua liu Yushun Gong National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals Hunan Agricultural UniversityChangsha 410128China Yunnan Key Laboratory of Tea Science Tea Research Institute of Yunnan Academy of Agricultural SciencesMenghai 666200China Key Laboratory of Tea Science of Ministry of Education Hunan Agricultural UniversityChangsha410128China

Fuzhuan brick-tea(FZT)has long been consumed for its supposed weight loss and lipid-lowering benefi *** this study,we show that the regulation of fat storage in Caenorhabditis elegans from a water extract of FZT was affected by cholesterol *** found that FZT signifi cantly decreased fat storage under normal cholesterol levels or in a cholesterol-free diet,while lipid accumulation was increased for a high cholesterol ***,this mechanism may involve the conserved sterol regulatory element-binding protein(SREBP)/mediator-15(MDT-15)signaling pathway and the nuclear hormone receptor *** addition,lipid synthesis-related genes inhibited by FZT were partially affected by a cholesterol-free ***,our fi ndings suggested that the potential lipid-lowering effects of FZT may depend on the cholesterol level,which may help to improve the consumption of FZT.

关键词： Fuzhuan brick tea Cholesterol Fat storage Sterol regulatory element-binding protein

Allometry-based estimation of forest aboveground biomass combining LiDAR canopy height attributes and optical spectral indexes

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Forest Ecosystems 2022年第5期9卷 617-629页

作者： Qiuli Yang Yanjun Su Tianyu Hu Shichao Jin Xiaoqiang liu Chunyue Niu zhonghua liu Maggi Kelly Jianxin Wei Qinghua Guo State Key Laboratory of Vegetation and Environmental Change Institute of BotanyChinese Academy of SciencesBeijing100093China University of Chinese Academy of Sciences Beijing100049China Plant Phenomics Research Centre Academy for Advanced Interdisciplinary StudiesCollaborative Innovation Centre for Modern Crop Production co-sponsored by Province and MinistryNanjing Agricultural UniversityNanjing210095China Department of Environmental Sciences Policy and ManagementUniversity of CaliforniaBerkeleyCA94720-3114USA Division of Agriculture and Natural Resources University of CaliforniaBerkeleyCA94720-3114USA College of Geography and Remote Sensing Sciences Xinjiang UniversityUrumqiXinjiang830017China Xinjiang Lidar Applied Engineering Technology Research Center UrumqiXinjiang830002China Xinjiang Land and Resources Information Center UrumqiXinjiang830002China Institute of Remote Sensing and Geographic Information System School of Earth and Space SciencesPeking UniversityBeijing100871China

Accurate estimates of forest aboveground biomass(AGB)are essential for global carbon cycle studies and have widely relied on approaches using spectral and structural information of forest canopies extracted from various remote sensing ***,combining the advantages of active and passive data sources to improve estimation accuracy remains ***,we proposed a new approach for forest AGB modeling based on allometric relationships and using the form of power-law to integrate structural and spectral *** 60 km^(2) of drone light detection and ranging(LiDAR)data and 1,370 field plot measurements,covering the four major forest types of China(coniferous forest,sub-tropical broadleaf forest,coniferous and broadleaf-leaved mixed forest,and tropical broadleaf forest),were collected together with Sentinel-2 images to evaluate the proposed *** results show that the most universally useful structural and spectral metrics are the average values of canopy height and spectral index rather than their maximum *** with structural attributes used alone,combining structural and spectral information can improve the estimation accuracy of AGB,increasing R^(2) by about 10%and reducing the root mean square error by about 22%;the accuracy of the proposed approach can yield a R^(2) of 0.7 in different forests *** proposed approach performs the best in coniferous forest,followed by sub-tropical broadleaf forest,coniferous and broadleaf-leaved mixed forest,and then tropical broadleaf ***,the simple linear regression used in the proposed method is less sensitive to sample size and outperforms statistically multivariate machine learning-based regression models such as stepwise multiple regression,artificial neural networks,and Random *** proposed approach may provide an alternative solution to map large-scale forest biomass using space-borne LiDAR and optical images with high accuracy.

关键词： Forest aboveground biomass Drone LiDAR Allometric relationship Power law Tree height Vegetation index

PD-L1 expression is regulated by ATP-binding of the ERBB3 pseudokinase domain

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Genes & Diseases 2023年第4期10卷 1702-1713页

作者： Yamu Li zhonghua liu Yiqing Zhao Jie Yang Tsan Sam Xiao Ronald A.Conlon Zhenghe Wang Department of Genetics and Genome Sciences Case Western Reserve UniversityClevelandOH 44106USA Case Comprehensive Cancer Center Case Western Reserve UniversityClevelandOH 44106USA Department of Pathology Case Western Reserve UniversityClevelandOH 44106USA Department of Physiology and Biophysics Case Western Reserve UniversityClevelandOH 44106USA Department of Integrative Structural and Computational Biology The Scripps Research Institute10550 North Torrey Pines RdTRY-21La JollaCA 92037USA.

How PD-L1 expression is regulated in cancer is poorly ***,we report that the ATP-binding activity of ERBB3 pseudokinase regulates PD-L1 gene expression in colorectal cancers(CRCs).ERBB3 is one of the four members of the EGF receptor family,all with protein tyrosine kinase ***3 is a pseudokinase with a high binding affin-ity to *** showed that ERBB3 ATP-binding inactivation mutant reduces tumorigenicity in genetically engineered mouse models and impairs xenograft tumor growth of CRC cell *** ERBB3 ATP-binding mutant cells dramatically reduce IFN-g-induced PD-L1 ***,ERBB3 regulates IFN-g-induced PD-L1 expression through the IRS1-PI3K-PDK1-RSK-CREB signaling *** is the transcription factor that regulates PD-L1 gene expression in CRC *** of a tumor-derived ERBB3 mutation located in the ki-nase domain sensitizes mouse colon cancers to anti-PD1 antibody therapy,suggesting that ERBB3 mutations could be predictive biomarkers for tumors amenable to immune check-point therapy.

关键词： Colon cancer ERBB3 Immunotherapy PD-L1 Pseudokinase