检索结果-南通市图书馆

Association between mitochondrial DNA haplotype compatibility and increased efficiency of bovine intersubspecies cloning

引用

Journal of Genetics and Genomics 2011年第1期38卷 21-28页

作者： Hao yan zhonghai yan Qingwen Ma Fei Jiao Shuzhen Huang Fanyi Zeng Yitao Zeng Shanghai Institute of Medical Genetics Shanghai Jiao Tong University 24/1400 West Beijing Road Shanghai 200040 China The Key Lab of Embryo Molecular Biology Ministry of Health China and Shanghai Lab of Embryo and Reproduction Engineering 24/1400 West Beijing Road Shanghai 200040 China Institute of Medical Science Shanghai Jiao Tong University School of Medicine 280 South Chongqing Road Shanghai 200025 China

Reconstructed embryos derived from intersubspecies somatic cell nuclear transfer （SCNT） have poorer developmental potential than those from intrasubspecies SCNT. Based on our previous study that Holstein dairy bovine （HD） mitochondrial DNA （mtDNA） haplotype compatibility between donor karyoplast and recipient cytoplast is crucial for SCNT embryo development, we performed intersubspecies SCNT using HD as donor karyoplast and Luxi yellow heifer （LY） as recipient cytoplast according to mtDNA haplotypes determined by polymerase chain reaction- restriction fragment length polymorphism （PCR-RFLP） analysis. The results demonstrated that intersubspecies mtDNA homotype SCNT embryos had higher pre- and post-implantation developmental competence than intrasubspecies mtDNA heterotype embryos as well as improved blastocyst reprogramming status, including normal H3K9 dimethylation pattern and promoter hypomethylation of pluripotent genes such as Oct4 and Sox2, suggesting that intersubspecies SCNT using LY oocytes maintains HD cloning efficiency and may reprogram HD nuclei to develop into a normal cloned animal ultimately. Our results indicated that karyoplast-cytoplast interactions and mtDNA haplotype compatibility may affect bovine intersubspecies SCNT efficiency. This study on bovine intersubspecies SCNT is valuable for understanding the mechanisms of mtDNA haplotype compatibility between karyoplast and cytoplast impacting the bovine SCNT efficiency, and provides an alternative and economic resource for HD cloning.

关键词： Somatic cell nuclear transfer (SCNT) Mitochondria mtDNA haplotype Epigenetic modification Bovine Intersubspecis Intrasubspecis Developmental competence

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

MiR-16-5p plays an inhibitory role in human non-small cell lung cancer through Fermitin family member 2

引用

BIOCELL 2021年第3期45卷 627-638页

作者： JUNQI GUO YUN yanG WEI ZHAO zhonghai yan XIA yanG YUNFEI yan RUIMIN HAO JINXIA HU FEI JIAO Department of Biochemistry and Molecular Biology Binzhou Medical UniversityYantai264003China Key Laboratory of Tumor Molecular Biology in Binzhou Medical University Yantai264003China Department of Neurology Columbia UniversityNew York10032USA Department of Cardiology The People’s Hospital of Zhaoyuan CityYantai265400China

Increasing evidence indicates that aberrant expressions of some microRNAs are associated with cancer ***,the roles and biological mechanisms of miRNA-16-5p in human non-small cell lung cancer(NSCLC)are not to be well ***,we validated that the expression of miR-16-5p was decreased significantly in NSCLC samples and cell *** correlation between the clinicopathological features of NSCLC and the miR-16-5p expression showed that the expression of miR-16-5p in non-small cell lung cancer was linked with the advanced TNM stage,positive lymph node metastasis,with short overall survival(OS).Also,a negative correlation between miR-16-5p and Fermitin family member 2(FERMT2)was observed,implying there may be a potential link about their *** hypothesis was further confirmed by in-silico analysis and dual-luciferase reporter ***,we demonstrated that the transfections of miR-16-5p mimics could alter some biological characteristics of NSCLC cells remarkably accomplished by the expression variance of FERMT2 in vitro and in vivo ***,this study demonstrated that miR-16-5p,as a tumor suppression factor in NSCLC by targeting FERMT2,could serve as one promising biomarker in the prediction for NSCLC patients.

关键词： miR-16-5p Non–small-cell lung cancer(NSCLC) Fermitin family member 2(FERMT2) Apoptosis Invasion Overall survival(OS)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Virtual source approach for maximizing resolution in high-penetration gamma-ray imaging

引用

Matter and Radiation at Extremes 2024年第3期9卷 19-30页

作者： Yuchi Wu Shaoyi Wang Bin Zhu Yonghong yan Minghai Yu Gang Li Xiaohui Zhang Yue yang Fang Tan Feng Lu Bi Bi Xiaoqin Mao zhonghai Wang Zongqing Zhao Jingqin Su Weimin Zhou Yuqiu Gu National Key Laboratory of Plasma Physics Laser Fusion Research CenterCAEPMianyangSichuan 621900China College of Physics Sichuan UniversityChengdu 610065China

High-energy gamma-ray radiography has exceptional penetration ability and has become an indispensable nondestructive testing(NDT)tool in various *** high-energy photons,point projection radiography is almost the only feasible imaging method,and its spatial resolution is primarily constrained by the size of the gamma-ray *** conventional industrial applications,gamma-ray sources are commonly based on electron beams driven by accelerators,utilizing the process of bremsstrahlung *** size of the gamma-ray source is dependent on the dimensional characteristics of the electron *** research has been conducted on various advanced accelerator technologies that have the potential to greatly improve spatial resolution in *** our investigation of laser-driven gamma-ray sources,a spatial resolution of about 90μm is achieved when the areal density of the penetrated object is 120 g/cm^(2).A virtual source approach is proposed to optimize the size of the gamma-ray source used for imaging,with the aim of maximizing spatial *** this virtual source approach,the gamma ray can be considered as being emitted from a virtual source within the convertor,where the equivalent gamma-ray source size in imaging is much smaller than the actual emission *** the basis of Monte Carlo simulations,we derive a set of evaluation formulas for virtual source scale and gamma-ray emission *** optimal conditions,the virtual source size can be as small as 15μm,which can significantly improve the spatial resolution of high-penetration imaging to less than 50μm.

关键词： resolution approach utilizing

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Application of Independent Component Analysis to the Aero-engine Fault Diagnosis

Application of Independent Component Analysis to the Aero-en...

引用

2009中国控制与决策会议

作者： Li zhonghai,Zhang yan,Jiang Liying,Qu Xiaoguang Shenyang Institute of Aeronautical Engineering,Shenyang 110136,China

＜正＞In view of the complexity of aero-engine system,there are massive correlated parameters,a novel method for aeroengine fault diagnosis based on Independent Component Analysis（ICA） is proposed in this *** is already widely applied in many domains,but the aeroengine fault diagnosis domain hasn’t *** is used to detect fault by calculating I statistics,and SVM models are constructed based on separate matrix of *** illustrate the efficiency of the proposed approach.

关键词： ICA SVM Fault detection Aeroengine fault diagnosis

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

四川盆地砂岩型铀矿远景预测及找矿方向

引用

地质论评 2016年第B11期62卷 29-30页

作者：邓祖林晏中海林洋庄景遗张亮核工业二八0研究所四川广汉618300

砂岩铀矿主要指形成于层间氧化还原过渡带的层间氧化型砂岩铀矿（王正邦，2002），一般产于中新生代盆地内，具品位低、储量大的特点（侯惠群等，2015）。四川盆地是西南地区最大的中生代盆地，铀矿成矿地质条件优越，其找矿前景备受关... 详细信息

砂岩铀矿主要指形成于层间氧化还原过渡带的层间氧化型砂岩铀矿（王正邦，2002），一般产于中新生代盆地内，具品位低、储量大的特点（侯惠群等，2015）。四川盆地是西南地区最大的中生代盆地，铀矿成矿地质条件优越，其找矿前景备受关注，其基底由上、下两部分组成，下部为前震旦纪结晶基底或褶皱基底，上部为震旦纪一中三叠世上扬子陆表海相碳酸盐岩和碎屑岩沉积建造，和盆地周边的火山岩、侵入岩组成了盆地的基底和蚀源区，广泛出露于盆地周边。

关键词：四川盆地砂岩型铀矿远景预测找矿方向

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

贵州龙宫风景名胜区水溶洞环境变化特征与预测研究

引用

热带地理 2012年第4期32卷 385-388页

作者：车家骧彭熙严忠海谢元贵李安定贵州科学院喀斯特资源环境与发展研究中心贵阳550001 龙宫风景名胜区管理委员会贵州安顺561021

对比研究了贵州龙宫风景区1996年和2011年15年前后客流量、温度、湿度和CO2浓度等有关数据,分析其环境变化特征,揭示影响因素,预测其发展趋势。结果表明:(1)洞内CO2浓度比洞外高,洞深越深CO2浓度越高,2011年CO2浓度比1996年显著增大,总... 详细信息

对比研究了贵州龙宫风景区1996年和2011年15年前后客流量、温度、湿度和CO2浓度等有关数据,分析其环境变化特征,揭示影响因素,预测其发展趋势。结果表明:(1)洞内CO2浓度比洞外高,洞深越深CO2浓度越高,2011年CO2浓度比1996年显著增大,总体说明游客数量对CO2浓度影响很大;(2)游客数量每增加1万人,则CO2浓度增加0.002%,年增加约0.0046%;大约经过13.5～18a,当年游客量达到135万人时,龙宫CO2浓度将超过CO2卫生二级标准;(3)各监测点各时段温度差异主要表现在洞外,洞内各监测点的各个时段基本无差异;(4)各监测点不同时段湿度无明显变化,仅存在洞内与洞外的差异。

关键词：环境变化水溶洞贵州龙宫

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

葡萄糖、木糖共发酵菌株发酵pH探索

引用

酿酒 2018年第2期45卷 102-104页

作者：宋思琦闫中海张宁中粮生化能源(肇东)有限公司黑龙江肇东151100

以纤维质为原料生产燃料乙醇能够缓解能源危机、减轻环境污染、推动农业的发展。纤维质原料含有大量的纤维素、半纤维素,XWS-N菌株能够利用其水解产生的葡萄糖和木糖共同发酵生成乙醇,提高乙醇得率。通过实验对比,初步确定XWS-N菌株发... 详细信息

以纤维质为原料生产燃料乙醇能够缓解能源危机、减轻环境污染、推动农业的发展。纤维质原料含有大量的纤维素、半纤维素,XWS-N菌株能够利用其水解产生的葡萄糖和木糖共同发酵生成乙醇,提高乙醇得率。通过实验对比,初步确定XWS-N菌株发酵最适pH为初始5.0过程中无需调节。在此条件下,乙醇得率可达到78.26%,与相同条件下单独发酵葡萄糖制乙醇相比,乙醇得率可提高8个百分点。

关键词：木质纤维素燃料乙醇葡萄糖木糖发酵

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

蛋白添加剂增强肉制品凝胶性研究进展

引用

食品科学 2024年第7期45卷 348-357页

作者：李志杰闫睿思汪秀娟胡中海蔡天赐甄宗圆安徽科技学院食品工程学院安徽滁州233100 霍邱县动物卫生监督所安徽六安237400 六安龙翔美食王禽业有限公司安徽六安237400 安徽杨府锦调味食品股份有限公司安徽淮北235000

凝胶性作为肉类食品的重要特性之一,是影响肉制品外观、风味、质构的重要因素,增强肉制品的凝胶性对于改善肉制品的工艺和品质具有重要意义。肉制品加工中蛋白质形成聚集体的含量、结构、聚集速率等都会影响肉制品的凝胶性,而蛋白质添... 详细信息

凝胶性作为肉类食品的重要特性之一,是影响肉制品外观、风味、质构的重要因素,增强肉制品的凝胶性对于改善肉制品的工艺和品质具有重要意义。肉制品加工中蛋白质形成聚集体的含量、结构、聚集速率等都会影响肉制品的凝胶性,而蛋白质添加剂可在一定程度上替代肌原纤维蛋白的凝胶和乳化作用,优化产品的感官和营养特性,并降低成本,成为肉制品研发和工艺优化的研究热点。本文对肌原纤维蛋白凝胶形成机理的研究进行汇总,并综述目前主要蛋白添加剂的性质和应用,以及蛋白添加剂与多糖胶体、酶制剂、磷酸盐进行复配增强肉制品凝胶性的研究进展,为凝胶肉制品的提质增效提供参考。

关键词：肉制品凝胶性蛋白添加剂复配凝胶机理

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

油茶籽多酚超声辅助提取的响应面优化

引用

食品与机械 2012年第1期28卷 166-170页

作者：朱晓阳钟海雁周波赵清洁汪阿虎中南林业科技大学食品科学与工程学院湖南长沙410004

采用Plackett-Burman(PB)筛选和中心组合设计(cen-tral composite design,CCD)对影响油茶籽多酚甲醇超声辅助提取过程的5个因素进行筛选及优化,确定最优超声辅助提取条件:提取温度50℃,料液比1∶38(m∶V),超声时间40min,甲醇体积分数60%... 详细信息

采用Plackett-Burman(PB)筛选和中心组合设计(cen-tral composite design,CCD)对影响油茶籽多酚甲醇超声辅助提取过程的5个因素进行筛选及优化,确定最优超声辅助提取条件:提取温度50℃,料液比1∶38(m∶V),超声时间40min,甲醇体积分数60%,pH值为7,该条件下油茶籽多酚提取量可达21.037 7mg/g。

关键词：油茶籽多酚超声辅助提取

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of Crustal Eclogitization on Plate Subduction/Collision Dynamics: Implications for India-Asia Collision

引用

Journal of Earth Science 2016年第5期27卷 727-739页

作者： Pengpeng Huangfu Yuejun Wang zhonghai Li Weiming Fan yan Zhang State Key Laboratory of Isotope Geochemistry Guangzhou Institute of Geochemistry Chinese Academy of Sciences Key Laboratory of Computational Geodynamics College of Earth Sciences University of Chinese Academy of Sciences School of Earth Science and Geological Engineering Sun Yat-Sen University CAS Center for Excellence in Tibetan Plateau Earth Sciences Key Laboratory of Continental Collision and Plateau UpliftInstitute of Tibetan Plateau Research Chinese Academy of Sciences

2D thermo-mechanical models are constructed to investigate the effects of oceanic and continental crustal eclogitization on plate dynamics at three successive stages of oceanic subduction, slab breakoff, and continental subduction. Crustal eclogitization directly increases the average slab density and accordingly the slab pull force, which makes the slab subduct deeply and steeply. Numerical results demonstrate that the duration time from initial continental collision to slab breakoff largely depends on the slab pull force. Specifically, eclogitization of subducted crust can greatly decrease the duration time, but increase the breakoff depth. The detachment of oceanic slab from the pro-continental lithosphere is accompanied with obvious exhumation of the subducted continental crust and a sharp uplift of the collision zone in response to the disappearance of downward drag force and the induced asthenospheric upwelling, especially under the condition of no or incomplete crustal eclogitization. During continental subduction, the slab dip angle is strongly correlated with eclogitization of subducted continental lower crust, which regulates the slab buoyancy nature. Our model results can provide several important implications for the Himalayan-Tibetan collision zone. For example, it is possible that the lateral variations in the degree of eclogitization of the subducted Indian crust might to some extent contribute to the lateral variations of subduction angle along the Himalayan orogenic belt. Moreover, the accumulation of highly radiogenic sediments and upper continental crustal materials at the active margin in combination with the strong shear heating due to continuous continental subduction together cause rising of isotherms in the accretionary wedge, which facilitate the development of crustal partial melting and metamorphism.

关键词： numerical modeling crustal eclogitization oceanic subduction slab breakoff continen-tal subduction Himalayan-Tibetan collision zone.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：