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Ideal type 1 is caused by a point mutation in the a-tubulin gene that affects microtubule arrangement in soybean

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The Crop Journal 2023年第3期11卷 713-722页

作者： Bohong Su Yong Guo zhongfeng li Huawei Gao Zhongyan Wei lijuan Qiu College of Agriculture Northeast Agricultural UniversityHarbin 150030HeilongjiangChina National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China College of Agronomy Henan Agricultural UniversityZhengzhou 450002HenanChina State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Key Laboratory of Biotechnology in Plant Protection of MARA of China and Zhejiang ProvinceInstitute of Plant VirologyNingbo UniversityNingbo 315211ZhejiangChina

Plant architecture is a target of crop *** soybean mutant ideal type 1(it1)displays a pleiotropic phenotype characterized by compact plant architecture,reduced plant height,shortened petioles,wrinkled leaves,and indented *** analysis revealed that the pleiotropic phenotype was controlled by an incomplete dominant *** characterized the cellular phenotypes of it1 and positionally cloned the it1 *** morphogenetic analysis of the it1 mutant revealed an excess of xylem cells and expanded phloem,and polygonal pavement *** cloning showed that the phenotype was caused by a G-to-A mutation in the second exon of the a-tubulin gene(Glyma.05G157300).The mutation altered microtubule arrangement in pavement cells,changing their *** of Gmit1 resulted in an it1-like phenotype and polygonal pavement cells and microtubules of overexpressors were parallel or slightly *** suppressor mutants able to suppress the phenotype of it1 were obtained by EMS mutagenesis in the it1 *** these mutants carried an additional mutation in the it1 *** results suggest that the pleiotropic phenotype of it1 is caused by the mutation in the atubulin gene.

关键词： Soybean Plant architecture a-Tubulin Microtubules Intragenic mutations

单孔非同轴脊柱内镜下经跨棘突入路治疗游离型腰椎间盘突出症的早期疗效

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中国修复重建外科杂志 2025年第1期39卷 83-87页

作者：李中锋刘晏东温力鹏陈博杨英王雨榕彭冉东宋恩甘肃省中医院脊柱疾病诊疗中心兰州730050 甘肃中医药大学研究生院兰州730000 临夏州人民医院骨科甘肃临夏730030 昆明医科大学第一附属医院运动医学科昆明650032

目的探讨采用单孔非同轴脊柱内镜(uni-portal non-coaxial spinal endoscopic surgery,UNSES)经跨棘突入路(crossing midline approach,CMA)治疗游离型腰椎间盘突出症(free lumbar disc herniation,FLDH)的早期疗效。方法2024年3月—6月,采用UNSES经CMA治疗16例FLDH患者。男9例,女7例;年龄47~62岁,平均55.1岁。病程8~30个月,平均15.6个月。病变节段:L3、44例,L4、55例,L5、S17例。术前疼痛视觉模拟评分(VAS)为(6.9±0.9)分,Oswestry功能障碍指数(ODI)为57.22%±4.16%。记录手术时间、术中出血量、术后住院时间以及相关并发症发生情况。术后采用VAS评分及ODI评价脊柱疼痛及功能情况,按照改良MacNab标准评价临床疗效;CT和MRI复查评价神经减压效果。结果16例患者手术均顺利完成,无手术相关并发症发生。手术时间63~81 min,平均71.0 min;术中出血量47.3~59.0 mL,平均55.0 mL;术后住院时间3~4 d,平均3.5 d。患者均获随访,随访时间1~3个月,其中随访达2个月15例、3个月14例。术后随时间延长VAS评分及ODI均逐渐降低,手术前后各时间点间比较差异均有统计学意义(P<0.05)。术后3个月,改良MacNab疗效评价达优12例、良2例,优良率达100%。随访期间影像学复查示椎管直径和横截面积明显增加,椎管有效减压。结论采用UNSES治疗FLDH时,选择CMA进行神经减压具有减压范围广、操作空间大以及操作自由等优势,可最大限度地保留关节突,避免峡部骨折断裂,清晰显示出口神经根和行走神经根,获得较好早期疗效。

关键词：单孔非同轴脊柱内镜跨棘突入路游离型腰椎间盘突出症早期疗效

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Identification and Fine Mapping of rhm1 Locus for Resistance to Southern Corn Leaf Blight in Maize

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Journal of Integrative Plant Biology 2012年第5期54卷 321-329页

作者： Yuanzeng Zhao Xiaomin Lu Chaoxian liu Haiying Guan Mei Zhang zhongfeng li Hongwei Cai Jinsheng Lai State Key Laboratory of Agrobiotechnology and National Maize Improvement Center Department of Plant Genetics and BreedingChina Agricultural UniversityBeijing 100193China Department of life Science and Technology Henan Institute of Science and TechnologyXinxiang 453003China Henan Academy of Agricultural Sciences Zhengzhou 450002China

rhml is a major recessive disease resistance locus for Southern corn leaf blight （SCLB）. To further narrow down its genetic position, F2 population and BCIFI population derived from the cross between resistant （H95rhm） and susceptible parents （H95） of maize （Zea mays） were constructed. Using newly developed markers, rhml was initially delimited within an interval of 2.5 Mb, and then finally mapped to a 8.56 kb interval between InDel marker IDP961-503 and simple sequence repeat （SSR） marker A194149--1. Three polymorphic markers IDP961-504, IDP B2-3 and A194149-2 were shown to be co-segregated with the rhml locus. Sequence analysis of the 8.56 kb DNA fragment revealed that it contained only one putative gene with a predicted amino acid sequence identical to lysine histidine transporter 1 （LHT1）. Comparative sequence analysis indicated that the LHT1 in H95rhrn harbors a 354 bp insertion in its third exon as compared with that of susceptible alleles in B73, H95 and Mo17. The 354 bp insertion resulted in a truncation of the predicted protein of candidate resistance allele （LHT1-H95rhm）. Our results strongly suggest LHTI as the candidate gene for rhml against SCLB. The tightly linked molecular markers developed in this study can be directly used for molecular breeding of resistance to Southern corn leaf blight in maize.

关键词： Bipolaris maydis fine-mapping maize rhml Southern corn leaf blight Zea mays.

不同亚型幽门螺杆菌感染与胃息肉在胃上皮内瘤变病理升级的交互作用

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传染病信息 2025年第1期38卷 62-69页

作者：李中峰孟敏徐烨吴晓晨鲁惠惠兖矿新里程总医院消化内科济宁273500

目的分析不同亚型幽门螺杆菌(Helicobacter pylori,Hp)感染与胃息肉在胃上皮内瘤变(gastric intraepithelial neoplasia,GIN)病理升级的交互作用。方法选取2021年1月至2024年1月于兖矿新里程总医院收治的200例GIN患者作为研究对象,其中... 详细信息

目的分析不同亚型幽门螺杆菌(Helicobacter pylori,Hp)感染与胃息肉在胃上皮内瘤变(gastric intraepithelial neoplasia,GIN)病理升级的交互作用。方法选取2021年1月至2024年1月于兖矿新里程总医院收治的200例GIN患者作为研究对象,其中,低级别上皮内瘤变(low-grade intraepithelial neoplasia,LGIN)118例,高级别上皮内瘤变(high-grade intraepithelial neoplasia,HGIN)82例。收集患者一般资料,分析各组GIN患者局部免疫状态白细胞介素(interleukin,IL)-6、IL-8、肿瘤坏死因子α(tumor necrosis factorα,TNF-α)、CD4^(+)T淋巴细胞、CD8^(+)T淋巴细胞水平,使用电子胃镜检查胃内病变情况,对黏膜进行组织活检,胃镜下取各部位及不同类型的息肉活组织或背景黏膜进行Hp感染检查,并依据Hp抗体检测结果进一步将Hp阳性分为HpⅠ型阳性和HpⅡ型阳性。采用多因素Logistic回归分析影响GIN病理升级的因素。分析Hp感染及伴有胃息肉对GIN病理升级的影响。采用广义多因子降维法(generalized multifactor dimensionality reduction,GMDR)分析多因素交互作用。结果LGIN 118例,HGIN 82例,LGIN中病理升级为HGIN 41例,HGIN中病理升级为胃癌(gastric cancer,GC)52例,病理升级患者共93例(46.50%),病理未升级患者共107例(53.50%)。在HGIN、GC患者中,HpⅠ型感染率高于HpⅡ型感染率;相比于HGIN组,GC组的HpⅠ型感染率增加;HpⅠ型、Ⅱ型感染率在HGIN、GC中的分布差异无统计学意义(P>0.05)。多因素Logistic回归分析显示,胃溃疡史、胃息肉、病变大小、异型血管、胃黏膜组织性病变与Hp感染是影响GIN病理升级的独立危险因素(P<0.05)。与病理未升级组相比,病理升级组的HGIN组Hp感染率、伴有胃息肉比率显著增加;在病理升级组中,GC组Hp感染率、伴有胃息肉比率显著高于HGIN组(P<0.001);在调整GIN相关因素后仍具有统计学意义(P<0.001)。趋势分析结果显示,随着GIN病变程度加重,Hp感染率、伴有胃息肉的OR值逐渐升高(P<0.001)。对5组患者胃局部免疫状态进行检测,结果显示,相对于Hp阴性组,HpⅠ型阳性组、HpⅡ型阳性组的IL-6、IL-8、TNF-α水平明显升高,CD4^(+)T淋巴细胞与CD8^(+)T淋巴细胞明显降低(P<0.05);相对于无胃息肉组,伴有胃息肉组IL-6、IL-8、TNF-α水平明显升高,CD4^(+)T淋巴细胞与CD8^(+)T淋巴细胞明显降低(P<0.05)。病理升级组胃局部的IL-6、IL-8、TNF-α水平显著高于病理未升级组,CD4^(+)T淋巴细胞与CD8^(+)T淋巴细胞计数显著低于病理未升级组(P<0.05)。将多因素分析结果引入GMDR模型分析,经置换检验结果显示,六阶交互模型为最佳模型,置换检验具有统计学意义(P<0.05)。结论不同亚型Hp感染与胃息肉不仅是影响GIN病理升级的独立危险因素,且2者联合对GIN病理升级具有交互作用,重点关注不同亚型Hp感染与胃息肉有利于GIN的早诊早治。

关键词：幽门螺杆菌感染胃息肉上皮内瘤变交互作用

Comprehensive analysis and selection of high oleic peanut varieties in China:A study on agronomic,yield,and quality traits

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Oil Crop Science 2024年第4期9卷 265-274页

作者： Fangping Gong Zhuo li Xiaojian Sun Yi Fan Yinghui liu Yurong Dang Hao li Xingguo Zhang Xingli Ma zhongfeng li Kai Zhao Dongmei Yin College of Agronomy Henan Agricultural UniversityZhengzhou450000China

Peanut(Arachis hypogaea L.)is an important oil *** acid is a major factor that determines the quality of ***,the high oleic and high oleic to linoleic acid ratio are the target traits in an advanced peanut breeding *** study provided an extensive evaluation of the genetic and physical characteristics as well as disease resistance of 220 high oleic peanut varieties in ***,these varieties clustered into five major categories based on their traits.A majority of these varieties have been bred using interspecific hybridization or selected from mutants of self-crossed parents,with the main parent varieties being Kaixuan 016 and *** of disease resistance showed that most high oleic peanut varieties could resist two or three ***,those varieties with resistance to multiple diseases were relatively ***,some high oleic peanut varieties showed no disease resistance or inadequate *** results further indicate that the genetic basis for high oleic peanut breeding is insufficient,highlighting the need for its further ***,our findings lay a critical foundation for future high oleic peanut breeding and promote better understanding of the genetic and trait diversity offered by these varieties.

关键词： Peanut High oleic acid Breeding Trait Genealogy

Genome-wide investigation of defensin genes in peanut(Arachis hypogaea L.)reveals AhDef2.2 conferring resistance to bacterial wilt

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The Crop Journal 2022年第3期10卷 809-819页

作者： Kai Zhao Rui Ren Xingli Ma Kunkun Zhao Chengxin Qu Di Cao Qian Ma Yingying Ma Fangping Gong zhongfeng li Xingguo Zhang Dongmei Yin College of Agronomy&Center for Crop Genome Engineering Henan Agricultural UniversityZhengzhou 450046HenanChina

Peanut(Arachis hypogea L.)bacterial wilt(BW)is a devastating disease caused by Ralstonia solanacearum that results in severe yield and quality *** defensins are short cysteine-rich peptides with antimicrobial *** role of defensin genes(Ah Def)in peanut is unclear.A genome-wide investigation of Ah Def genes was undertaken,and 12 identified Ah Def genes were classified into two groups containing the gamma-thionin domain formed by four disulfide pairs:Cys1-Cys8,Cys2-Cys5,Cys3-Cys6,and *** silico analysis revealed that Ah Def genes showed highly conserved architectural features and contained cis-elements associated with phytohormone signaling and defense responses.A highly resistant cultivar,H108(R)and a susceptible accession,H107(S)were tested by *** inoculation.H108(R)showed fewer symptoms than H107(S)owing to inhibition of bacterial reproduction and spread in the vascular bundles of roots and *** a transcriptomic expression profile,Ah Def genes,particularly Ah Def1.6 and Ah Def2.2,were up-regulated in H108(R)compared with H107(S)under *** infection and phytohormone *** localization showed that the Ah Def1.6 and Ah Def2.2 proteins were both expressed specifically on the plasma *** of protein fusion Ah Def2.2-YFP in Nicotiana benthamiana and peanut leaves increased resistance to ***,suggesting its role in response to BW *** Def2.2 may be valuable for peanut resistance breeding.

关键词： Peanut Bacterial wilt Defensin Expression analysis Resistance

Electron Energy Spectroscopic Mapping of Surface Plasmon by Parallel Scanning Method

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Chinese Journal of Chemical Physics 2022年第3期35卷 537-541,I0003页

作者： zhongfeng li Tuo liu Chunkai Xu Xiangjun Chen Hefei National Research Center for Physical Sciences at the Microscale and Department of Modern Physics University of Science and Technology of ChinaHefei 230026China

In this work,electron energy spectroscopic mapping of surface plasmon of Ag nanostructures on highly oriented pyrolytic graphite is *** from the angular dispersive feature of the present scanning probe electron energy spectrometer,a multi-channel detection mode is *** scanning along one direction,the two-dimensional intensity distribution of Ag surface plasmon excitation due to the collision of electron emitted from the tip can be obtained in *** spectroscopic spatial resolution is determined to be around 80 nm.

关键词： Scanning probe electron energy spectroscopy Surface plasmon Spectroscopic mapping

Mushroom Poisoning Outbreaks—China,2023

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China CDC weekly 2024年第4期6卷 64-68,I0001-I0005页

作者： Haijiao li Yizhe Zhang Hongshun Zhang Jing Zhou Zuohong Chen Jiaqi liang Yu Yin Qian He Shaofeng Jiang Yutao Zhang Yuan Yuan Nan Lang Bowen Cheng Jiaju Zhong zhongfeng li Chengye Sun National Institute of Occupational Health and Poison Control Chinese Center for Disease Control and PreventionBeijingChina College of life Sciences Hunan Normal UniversityChangsha CityHunan ProvinceChina

What is already known about this topic?Mushroom poisoning poses a significant food safety concern in China,with a total of 196 species identified in poisoning incidents by the end of *** is added by this report?In 2023,the China CDC conducted an investigation into 505 cases of mushroom poisoning spanning 24 provincial-level administrative *** investigation resulted in 1,303 patients and 16 deaths,yielding a case fatality rate of 1.23%.A total of 97 mushrooms were identified as the cause of 6 distinct clinical disease types,with 12 species newly documented as poisonous mushrooms in *** are the implications for public health practice?Close collaboration among CDC staff,physicians,and mycologists remains crucial for the control and prevention of mushroom poisoning in the future.

关键词： prevention yielding poisoning

Loss of the accessory chromosome converts a pathogenic tree-root fungus into a mutualistic endophyte

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Plant Communications 2024年第1期5卷 164-180页

作者： Huanshen Wei Zhenhui Zhong zhongfeng li Yuwei Zhang Eva H.Stukenbrock Boping Tang Ningning Yang Riccardo Baroncelli Long Peng Zhuo liu Xinghua He Yuzhan Yang Zhilin Yuan State Key Laboratory of Tree Genetics and Breeding Chinese Academy of ForestryBeijing 100091China Research Institute of Subtropical Forestry Chinese Academy of ForestryHangzhou 311400China State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Fujian Agriculture and Forestry UniversityFuzhou 350002China Environmental Genomics Christian-Albrechts University24118 KielGermany Max Planck Fellow Group Environmental Genomics Max Planck Institute for Evolutionary Biology24306 PlonGermany Jiangsu Key Laboratory for Bioresources of Saline Soils School of WetlandsYancheng Teachers UniversityYancheng 224002China Department of Agricultural and Food Sciences(DISTAL) University of Bologna40127 BolognaItaly

Some fungal accessory chromosomes(ACs)may contribute to virulence in ***,the mecha-nisms by which ACs determine specific traits associated with lifestyle transitions along a symbiotic contin-uum are not *** we delineated the genetic divergence in two sympatric but considerably variable isolates(16B and 16W)of the poplar-associated fungus Stagonosporopsis *** identified a-0.6-Mb horizontally acquired AC in 16W that resulted in a mildly parasitic lifestyle in *** deletion of the AC(D16W)significantly altered the fungal ***,D16W was morphologically more similar to 16B,showed enhanced melanization,and established beneficial interactions with poplar plants,thereby acting as a dark septate *** sequencing(RNA-seq)analysis showed that AC loss induced the upregulation of genes related to root colonization and biosynthesis of indole acetic acid and *** observed that the AC maintained a more open status of chromatin across the genome,indicating an impressive remodeling of cis-regulatory elements upon AC loss,which potentially enhanced symbiotic *** demonstrated that the symbiotic capacities were non-host-specific through comparable experiments on Triticum–and Arabidopsis–fungus ***,the three isolates generated symbiotic interactions with a nonvascular *** summary,our study suggests that the AC is a suppressor of symbiosis and provides insights into the underlying mechanisms of mutualism with vascular plants in the absence of traits encoded by the *** speculate that AC-situ-ated effectors and other potential secreted molecules may have evolved to specifically target vascular plants and promote mild virulence.

关键词： dark septate endophytes effectors lifestyle transition root–fungus symbioses