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Investigating the function of Akt by tet-off inducible expression system

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Chinese Science Bulletin 2001年第3期46卷 222-225页

作者： Qiwei Zhai Hongbin Ji zhongcheng zheng Lanying Sun Xinyuan Liu Chinese Acad Sci Shanghai Inst Biol Sci Inst Biochem & Cell Biol Shanghai 200031 Peoples R China

A tet-off inducible cell line named BBT derived from BA/F3β cell line was constructed and the effect of this inducible expression system was significant when detected by tet-off responded luciferase reporter gene assay. Then tet-off responded Akt expression plasmid was transfected into BBT cells, and the stable cell lines were screened. The result of Northern blot showed that the expression of akt was significantly inducible. The clone with the best inducible effect was selected and named BBA for investigating the function of Akt. We found that Akt could significantly inhibit zinc-induced decrease of cell viability when assayed by MTT method. And the flow cytometric analysis showed that Akt could markedly repress zinc-induced apoptosis.

关键词： inducible expression system Akt apoptosis.

Study on the interaction between Jak3 and IL-2R γ using the yeast two-hybrid system

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Chinese Science Bulletin 1999年第18期44卷 1664-1669页

作者： Jinfang Zhu Wei Tang Hongbin Ji zhongcheng zheng Xinyuan Liu Shanghai Institute of Biochemistry Chinese Academy of Sciences Shanghai China

Both interleukin-2 (IL-2) receptor y subunit and non-receptor tyrosine kinase Jak3 play important roles in IL-2 physiological functions. Jak3 has been known to bind IL-2Ry and the interaction is very important for IL-2 signaling. In order to find the domains directly involved in the interaction between IL-2Ry and Jak3, various deletion mutants have been constructed

关键词： IL-2Rγ Jak3 yeast two-hybrid system interaction.

JAK3 mediates c-myc gene expression induced by interleukin-2

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Chinese Science Bulletin 1998年第4期43卷 308-311页

作者： Zhu Jinfang Lu Linrong Ji Hongbin zheng zhongcheng J. Thèze Liu Xinyuan Chinese Acad Sci Shanghai Inst Biochem Shanghai 200031 Peoples R China Inst Pasteur Paris France

C myc gene expression can be rapidly induced by IL 2 through intracellular signal transduction which is triggered by the interaction between IL 2 and its receptor （IL 2R）. JAK3 which associates to the intracellular domain of IL2R γ may play a critical role in this process. To reveal the action of JAK3 in c myc induction, a chimeric receptor gene IL2R α/γ/Δ NJAK3 is constructed which consists of extracellular domain derived from IL 2R α subunit （IL2R α）, the transmembrane sequence derived from IL2R γ and the cytoplasmic domain derived from the catalytic domain of JAK3 （Δ NJAK3）, and then transfected this chimeric gene into mouse fibroblast cell line L929 β which had been transfected with IL2R β gene and stably expressed IL2R β in high level. In the transfectants coexpressing IL2R β and α/γ/Δ NJAK3, the stimulation of IL2 could intensively induce c myc gene expression. Because the whole cytoplasmic domain of IL2R γ which could recruit signaling molecules was replaced by JAK3 and the c myc could be still induced by IL2 in this situation, the results here gave the direct evidence demonstrating that JAK3 plays an important role in c myc gene expression induced by IL2.

关键词： interleukin 2(IL 2) JAK3(Janus Kinase 3) c myc gene signal transduction.

Interleukin-2 mediates the peripheral analgesia of interferon alpha

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Chinese Science Bulletin 1999年第3期44卷 243-246页

作者： Li Li Qiwei Zhai Wei Tang Tong Wu zhongcheng zheng Xinyuan Liu Chinese Acad Sci Shanghai Inst Biochem Shanghai 200031 Peoples R China

Interferon-α (IFN-α) and interleukin-2 (IL-2) are crucial cytokines in immune system. They also play an important role in nerve system. It has been reported that IL-2 can induce the central analgesia. It is demonstrated that IFN-α also can induce the peripheral analgesia, which can be blocked by naloxone as IL-2. Furthermore, the analgesic effect of IFN-α is reversible by monoclonal anti-IL-2 antibody. In vitro experiments show that IFN-α significantly increases the production of IL-2 in a dose dependent manner. These data suggest that IL-2 mediates the peripheral analgesia of IFN-α.

关键词： IFN-α IL-2 analgesia.

超临界水冷堆燃料包壳材料的辐照损伤研究进展

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原子核物理评论 2017年第2期34卷 211-218页

作者：郑中成郭立平唐睿武汉大学物理科学与技术学院武汉430072 中国核动力研究设计院成都610041

超临界水冷堆(SCWR)是第四代核电站的主力堆型之一,高温、高压、超临界水环境下的辐照损伤问题是其燃料包壳材料面临的最大挑战。SCWR燃料包壳候选材料主要包括锆合金、奥氏体不锈钢、铁素体/马氏体不锈钢、镍基合金、ODS合金五大类,奥... 详细信息

超临界水冷堆(SCWR)是第四代核电站的主力堆型之一,高温、高压、超临界水环境下的辐照损伤问题是其燃料包壳材料面临的最大挑战。SCWR燃料包壳候选材料主要包括锆合金、奥氏体不锈钢、铁素体/马氏体不锈钢、镍基合金、ODS合金五大类,奥氏体不锈钢是最有希望的候选材料。介绍了近年来在这个领域国际上的主要研究进展。作者所在团队也对多种SCWR的候选材料进行了辐照损伤研究,包括:镍基合金C-276和718、铁素体/马氏体钢P92、奥氏体不锈钢AL-6XN和HR3C。对AL-6XN的氢离子辐照实验发现,辐照产生的缺陷主要是间隙型位错环,伯格斯矢量为1/3,在较高剂量(5～7 dpa)辐照下,出现空洞肿胀。在氢滞留的影响下,位错环有着独特的演化规律,总结提出了位错环的四阶段演化过程。

关键词：超临界水冷堆燃料包壳材料辐照损伤中子辐照

Predominant expression of β-chain variable region genes of T-cell receptor in TILs from human renal cell carcinoma

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Chinese Science Bulletin 1997年第8期42卷 693-697页

作者： LI Xueyi, WANG Ying, zheng zhongcheng, ZHANG Dongqing, FUSaili, LING Jianyu and ZHOU Guangyan (CHOU Kuangyen)1. Shanghai Institute of Immunology, Shanghai Second Medical University, Shanghai 200025, China 2. Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai 200031, China 3. Shanghai Ruijin Hospital, Shanghai 200025, China Shanghai Institute of Immunology Shanghai Second Medical University Shanghai China Shanghai Institute of Biochemistry Chinese Academy of Sciences Shanghai China Shanghai Ruijin Hospital Shanghai China

THE repertoire of T-cell antigen receptor(TcR)can be effectively analysed by an approachwith quantitative polymerase chain reaction(qPCR),which allows to characterize the ex-panding T-cell clones by the patterns of re... 详细信息

关键词： renal cell carcinoma tumor-infiltrating lymphocyte T-cell receptor Vβ gene quantitative polymerase chain reaction.

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氢氦离子辐照对4H-SiC表面形貌的影响

武汉大学学报（理学版） 2017年第4期63卷 342-348页

作者：刘月郑智张伟平郑中成沈震宇郭立平武汉大学物理科学与技术学院湖北武汉430072

以4H-SiC为研究对象,在800℃下,用24keV的H^+和30keV的He^+分别进行H^+单束、He^+单束、先H^+后He^+双束和先He^+后H^+双束辐照,并利用原子力显微镜(AFM)研究辐照对4H-SiC表面形貌的影响.实验表明:H^+辐照的样品表面出现直径平均约为8μ... 详细信息

以4H-SiC为研究对象,在800℃下,用24keV的H^+和30keV的He^+分别进行H^+单束、He^+单束、先H^+后He^+双束和先He^+后H^+双束辐照,并利用原子力显微镜(AFM)研究辐照对4H-SiC表面形貌的影响.实验表明:H^+辐照的样品表面出现直径平均约为8μm的大凸起,He^+辐照的样品表面则产生了均匀的纳米尺寸的小凸起;H^+辐照在材料表面产生的大凸起在辐照He^+后消失;而He^+预辐照之后再进行H^+辐照,材料表面不会产生大的凸起,并结合X射线衍射(XRD)数据对这种氢氦协同效应进行了解释.结论表明,He^+预辐照对凸起的形成有抑制作用,He^+后辐照则对已产生的凸起有抛光作用.

关键词： 4H-SiC 离子辐照氢氦协同作用

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组织专一性表达自杀基因治疗人结肠癌的研究

生物化学与生物物理进展 1996年第4期23卷 376-378页

作者：蒋琼戈凯许德华郑仲承刘新垣中国科学院上海生物化学研究所

构建了以癌胚抗原（ＣＥＡ）基因启动子控制的ＨＳＶ－ＴＫ和ＥＣＣＤ的表达质粒ＰＣＥＡ－ＴＫ和ｐＣＥＡ－ＣＤ．将它们分别与ｐＳＶ２－ｎｅｏ共转染人结肠癌细胞株ＬｏＶｏ和人宫颈癌细胞株ＨｅＬａ，Ｇ４１８筛选得到细胞克隆Ｌｏ... 详细信息

构建了以癌胚抗原（ＣＥＡ）基因启动子控制的ＨＳＶ－ＴＫ和ＥＣＣＤ的表达质粒ＰＣＥＡ－ＴＫ和ｐＣＥＡ－ＣＤ．将它们分别与ｐＳＶ２－ｎｅｏ共转染人结肠癌细胞株ＬｏＶｏ和人宫颈癌细胞株ＨｅＬａ，Ｇ４１８筛选得到细胞克隆ＬｏＶｏ／ＣＥＡ－ＴＫ、ｔｏＶｏ／ＣＥＡ－ＣＤ、ＨｅＬａ／ＣＥＡ－ＴＫ和ＨｅＬａ／ＣＥＡ－ＣＤ．与野生型ＬｏＶｏ细胞相比，ＬｏＶｏ／ＣＥＡ－ＴＫ和ＬｏＶｏ／ＣＥＡ－ＣＤ形态无明显改变，生长曲线也相似，但对ＧＣＶ或５－ＦＣ的细胞毒的敏感性分别提高了２０００倍或７００信而ＨｅＬａ／ＣＥＡ－ＴＫ（或ＨｅＬａ／ＣＥＡ－ＣＤ）仍对低浓度ＧＣＶ（或５－ＦＣ）不敏感．以上结果显示了应用组织专一性表达的自杀基因治疗人结肠癌的可能性．

关键词：结肠肿瘤癌自然基因基因治疗

hGM-CSF 基因转导的人胃癌、肝癌细胞研究

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中华微生物学和免疫学杂志 1997年第3期17卷 218-221页

作者：王立群戈凯郑仲承钱关祥刘新垣陈诗书上海第二医科大学人类基因治疗中心中科院上海生化所

ｈＧＭ－ＣＳＦ是一种细胞因子，能通过多种机制激活机体免疫功能。为了研究ｈＧＭ－ＣＳＦ修饰的人肝癌、胃癌细胞的免疫功能的变化，将含该基因的逆转录病毒载体ＬＸＳＮ转染ＰＡ３１７包装细胞，经Ｇ４１８筛选较高滴度病毒分泌株（... 详细信息

ｈＧＭ－ＣＳＦ是一种细胞因子，能通过多种机制激活机体免疫功能。为了研究ｈＧＭ－ＣＳＦ修饰的人肝癌、胃癌细胞的免疫功能的变化，将含该基因的逆转录病毒载体ＬＸＳＮ转染ＰＡ３１７包装细胞，经Ｇ４１８筛选较高滴度病毒分泌株（滴度为１．５×１０４ＣＦＵ／ｍｌ），用病毒上清感染肝癌（ＨＨＣＬ）和胃癌（ＭＫＮ４５），筛选后测ｈＧＭ－ＣＳＦ活性，最高分别为２７１Ｕ／１０６细胞·２４小时和２４３．８０Ｕ／１０６细胞·２４小时。ｈＧＭ－ＣＳＦ基因在肝、胃癌细胞中整合，未发生重排。ＭＨＣ分子测定结果显示经修饰的肝癌ＨＨＣＬ细胞ＭＨＣ分子的表达无改变，而修饰后胃癌细胞ＭＫＮ４５的ＭＨＣⅠ类分子表达明显增加。本实验为今后瘤苗研制提供了资料。

关键词：基因治疗胃肿瘤治疗肝肿瘤 hGM-CSF