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Testing complete plastomes and nuclear ribosomal DNA sequences for species identification in a taxonomically difficult bamboo genus Fargesia

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Plant Diversity 2023年第2期45卷 147-155页

作者： Shi-Yu Lv Xia-Ying Ye zhong-hu li Peng-Fei Ma De-Zhu li School of life Science Northwest UniversityXi'anShaanxi710069China Germplasm Bank of Wild Species Kunming Institute of BotanyChinese Academy of SciencesKunmingYunnan650201China Agronomy and life Science Department Zhaotong UniversityZhaotongYunnan657000China Kunming College of life Science University of Chinese Academy of SciencesKunmingYunnan650201China

Fargesia,the largest genus within the temperate bamboo tribe Arundinarieae,has more than 90 species mainly distributed in the mountains of Southwest *** Fargesia bamboos are important components of the subalpine forest ecosystems that provide food and habitat for many endangered animals,including the giant ***,species-level identification of Fargesia is ***,the rapid radiation and slow molecular evolutionary rate of Fargesia pose a significant challenge to using DNA barcoding with standard plant barcodes(rbcL,matK,and ITS) in *** progress in the sequencing technologies,complete plastid genomes(plastomes) and nuclear ribosomal DNA(nrDNA)sequences have been proposed as organelle barcodes for species identification;however,these have not been tested in *** collected 196 individuals representing 62 species of Fargesia to comprehensively evaluate the discriminatory power of plastomes and nrDNA sequences compared to standard *** analysis indicates that complete plastomes have substantially higher discriminatory power(28.6%) than standard barcodes(5.7%),whereas nrDNA sequences show a moderate improvement(65.4%) compared to ITS(47.2%).We also found that nuclear markers performed better than plastid markers,and ITS alone had higher discriminatory power than complete *** study also demonstrated that plastomes and nrDNA sequences can contribute to intrageneric phylogenetic resolution in ***,neither of these sequences were able to discriminate all the sampled species,and therefore,more nuclear markers need to be identified.

关键词： Fargesia Genome-skimming DNA barcoding Plastome Ribosomal DNA

Visualization and Characterization of High-Order Chromatin Fibers under light Microscope during Interphase and Mitotic Stages in Plants

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Journal of Integrative Plant Biology 2007年第11期49卷 1634-1639页

作者： Jing-Yu liu Chao-Wen She zhong-li hu Fen li Ying Diao li-hua liu Yun-Chun Song human Genome Research Center and College of life Science and Technology Huazhong University of Science and Technology Wuhan 430074 China Key Laboratory of the Ministry of Education of Plant Developmental Biology Wuhan University Wuhan 430072 China Department of Biology Huaihua College Huaihua 418008 China

Using genomic in situ hybridization with genomic DNA, high-order chromatin fibers were successfully exhibited under a light microscope through the cell cycle in barley, rice, maize and field bean. From the interphase to prophase and metaphase of mitosis, the fibers were basically similar. Each was estimated to be around 200 nm in diameter, but the strength of signals was not the same along the fiber length. Through the cell cycle a series of dynamic distribution changes occurred in the fibers. In the interphase, they were unraveled. At the early prophase they were arranged with parallel and mirror symmetry. During late-prophase and metaphase, the fibers were bundled and became different visible chromosomes. The parallel coiling and mirror symmetry structures were visible clearly until the metaphase. In anaphase they disappeared. During telophase, in peripheral regions of congregated chromosome group, borderlines of the chromosomes disappeared and the fibers were unraveled. This demonstrated that mitotic chromosomes are assembled and organized by parallel and adjacent coiling of the fibers and the fibers should be the highest order structure for DNA coiling.

关键词： high-order chromatin fibers interphase mitosis.

Transcriptome analysis of the endangered Notopterygium incisum: Coldtolerance gene discovery and identification of EST-SSR and SNP markers

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Plant Diversity 2019年第1期41卷 1-6页

作者： Yun Jia Ji-Qing Bai Mi-li liu Zhen-Fang Jiang Yan Wu Min-Feng Fang zhong-hu li Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of Education College of Life Sciences Northwest University Shaanxi University of Chinese Medicine

Notopterygium incisum C. C. Ting ex H. T. Chang(Apiaceae) is an endangered perennial herb in China. The lack of transcriptomic and genomic resources for N. incisum greatly hinders studies of its population genetics and conservation. In this study, we employed RNA-seq technology to characterize transcriptomes for the flowers, leaves, and stems of this endangered herb. A total of 56 million clean reads were assembled into 120,716 unigenes with an N50 length of 850 bp. Among these unigenes, 70,245(58.19%) were successfully annotated and 65,965(54.64%) were identified as coding sequences based on their similarities with sequences in public databases. We identified 21 unigenes that had significant relationships with cold tolerance in N. incisum according to gene ontology(GO) annotation analysis. In addition, 13,149 simple sequence repeats(SSRs) and 85,681 single nucleotide polymorphisms were detected as potential molecular genetic markers. Ninety-six primer pairs of SSRs were randomly selected to validate their amplification efficiency and polymorphism. Nineteen SSR loci exhibited polymorphism in three natural populations of N. incisum. These results provide valuable resources to facilitate future functional genomics and conservation genetics studies of N. incisum.

关键词： Endangered species EST-SSR marker Notopterygium incisum Single nucleotide polymorphism Transcriptome

Genetic structure,gene flow pattern,and association analysis of superior germplasm resources in domesticated upland cotton(Gossypium hirsutum L.)

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Plant Diversity 2020年第3期42卷 189-197页

作者： Ting-Ting Zhang Na-Yao Zhang Wei li Xiao-Jian Zhou Xiao-Yu Pei Yan-Gai liu zhong-Ying Ren Kun-Lun He Wen-Sheng Zhang Ke-Hai Zhou Fei Zhang Xiong-Feng Ma Dai-Gang Yang zhong-hu li Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of EducationCollege of Life SciencesNorthwest UniversityXi'an710069China State Key Laboratory of Cotton Biology Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang455000China

Gene flow patterns and the genetic structure of domesticated crops like cotton are not well ***,marker-assisted breeding of cotton has lagged far behind that of other major crops because the loci associated with cotton traits such as fiber yield and quality have scarcely been *** this study,we used 19 microsatellites to first determine the population genetic structure and patterns of gene flow of superior germplasm resources in upland *** then used association analysis to identify which markers were associated with 15 agronomic traits(including ten yield and five fiber quality traits).The results showed that the upland cotton accessions have low levels of genetic diversity(polymorphism information content=0.427),although extensive gene flow occurred among different ecological and geographic *** clustering analysis indicated that the cotton resources used in this study did not belong to obvious geographic populations,which may be the consequence of a single source of domestication followed by frequent genetic introgression mediated by human transference.A total of 82 maker-trait associations were examined in association analysis and the related ratios for phenotypic variations ranged from 3.04% to 47.14%.Interestingly,nine SSR markers were detected in more than one environmental *** addition,14 SSR markers were co-associated with two or more different *** was noteworthy that NAU4860 and NAU5077 markers detected at least in two environments were simultaneously associated with three fiber quality traits(uniformity index,specific breaking strength and micronaire value).In conclusion,these findings provide new insights into the population structure and genetic exchange pattern of cultivated cotton *** quantitative trait loci of domesticated cotton identified will also be very useful for improvement of yield and fiber quality of cotton in molecular breeding programs.

关键词： Domestication cotton Fiber quality traits Genetic exchange Microsatellite markers Yield

Exploring the performance of large language models on hepatitis B infection-related questions:A comparative study

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World Journal of Gastroenterology 2025年第3期31卷 103-112页

作者： Yu li Chen-Kai huang Yi hu Xiao-Dong Zhou Cong He Jia-Wei zhong Department of Gastroenterology Jiangxi Provincial Key Laboratory of Digestive DiseasesJiangxi Clinical Research Center for GastroenterologyDigestive Disease HospitalThe First Affiliated HospitalJiangxi Medical CollegeNanchang UniversityNanchang 330006Jiangxi ProvinceChina huanKui Academy Nanchang UniversityNanchang 330006Jiangxi ProvinceChina

BACKGROUND Patients with hepatitis B virus(HBV)infection require chronic and personalized care to improve *** language models(LLMs)can potentially provide medical information for *** To examine the performance of three LLMs,ChatGPT-3.5,ChatGPT-4.0,and Google Gemini,in answering HBV-related *** LLMs’responses to HBV-related questions were independently graded by two medical professionals using a four-point accuracy scale,and disagreements were resolved by a third *** question was run three times using three *** was assessed via the Gunning Fog index and Flesch-Kincaid grade *** Overall,all three LLM chatbots achieved high average accuracy scores for subjective questions(ChatGPT-3.5:3.50;ChatGPT-4.0:3.69;Google Gemini:3.53,out of a maximum score of 4).With respect to objective questions,ChatGPT-4.0 achieved an 80.8%accuracy rate,compared with 62.9%for ChatGPT-3.5 and 73.1%for Google *** the six domains,ChatGPT-4.0 performed better in terms of diagnosis,whereas Google Gemini demonstrated excellent clinical ***,in the readability analysis,the mean Gunning Fog index and Flesch-Kincaid grade level scores of the three LLM chatbots were significantly higher than the standard level eight,far exceeding the reading level of the normal *** Our results highlight the potential of LLMs,especially ChatGPT-4.0,for delivering responses to HBV-related *** may be an adjunctive informational tool for patients and physicians to improve ***,current LLMs should not replace personalized treatment recommendations from physicians in the management of HBV infection.

关键词： ChatGPT-3.5 ChatGPT-4.0 Google Gemini Hepatitis B infection Accuracy

Risk factors for non-sentinel lymph node metastases in breast cancer patients with positive sentinel lymph nodes

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Chinese Medical Journal 2008年第20期121卷 2107-2109页

作者： ZHANG Tao WANG Hong CHEN Bao-ping ZHANG Hai-song WEI Xi-liang FU Ying li zhong hu Geng-kun Department of Oncology Surgery Affiliated Hospital of Hebei University Baoding Hebei 071000 China

The presence or absence of metastases in the axillary lymph nodes has remained the most powerful prognostic factor in breast carcinoma. Axillary lymph node dissection （ALND） is the standard procedure for obtaining this information. However, postoperative complications are common. Sentinel lymph node （SLN） biopsy has been proposed as a potential alternative to ALND for staging breast carcinoma. This technique has been shown to be sensitive and specific for predicting the status of the axillary lymph nodes. Furthermore, it has the potential advantage of decreasing the morbidity associated with ALND.

关键词： breast cancer sentinel lymph node biopsy non-sentinel lymph node metastasis

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我国绿色税收政策效果分析及改革设想

生产力研究 2011年第9期 63-64,103页

作者：王鑫李忠华沈阳大学经济学院辽宁沈阳110041

我国在经济快速发展过程中出现了许多社会问题,其中环境污染是最严峻的问题之一。税收政策在促进环境改善方面具有十分明显的作用,通过制定税收政策引导企业经营方式的改变,从而实现环境改善已是各国的通行作法。与欧美国家相比我国税... 详细信息

我国在经济快速发展过程中出现了许多社会问题,其中环境污染是最严峻的问题之一。税收政策在促进环境改善方面具有十分明显的作用,通过制定税收政策引导企业经营方式的改变,从而实现环境改善已是各国的通行作法。与欧美国家相比我国税制的绿化程度很低,且不成体系。与生态环境有关的税收政策,散见于资源税、消费税、企业所得税等税种中,许多仍以费的形式存在,有较大的空间。应在借鉴国外绿色税收政策成功作法和经验的基础上,增加现行税种的环境保护功能,实行专门的环保税种,实行费改税等。

关键词：绿色税收环境保护费改税

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电容式电动角行程执行机构位置发送器设计

实验室研究与探索 2013年第1期32卷 33-34,73页

作者：李忠虎闫丹内蒙古科技大学信息工程学院内蒙古包头014010

设计了一种以电容传感器为敏感元件的电动角行程执行机构位置发送器。敏感元件采用基于比例测量原理的分瓣三层平行板电容式角位移传感器,主机系统以PIC16F877单片机为核心构建。结合现有电动角行程执行机构的内部结构和工程应用,利用AN... 详细信息

设计了一种以电容传感器为敏感元件的电动角行程执行机构位置发送器。敏感元件采用基于比例测量原理的分瓣三层平行板电容式角位移传感器,主机系统以PIC16F877单片机为核心构建。结合现有电动角行程执行机构的内部结构和工程应用,利用ANSYS软件对敏感元件进行了有限元分析和仿真研究,并给出了位置发送器的具体实施方案。以电容式角位移传感器取代过去的差动变压器,使位置发送器的体积明显减小,设备调试工作也变得更为简单,而且,其测量精度也有一定的提高。

关键词：电动执行机构位置发送器角位移测量比例测量原理电容传感器

基于MSP430的小口径低功耗超声波流量计设计

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仪表技术与传感器 2013年第11期 19-21页

作者：李忠虎沈侃内蒙古科技大学信息工程学院

超声波流量计是目前应用较广泛的一种流量计量设备,它以非接触方式进行流量计量,具有良好的应用前景。文中设计了一种基于超低功耗16位单片机MSP430微控制器的时差式超声波流量计,采用具有温度测量功能的高精度时间测量芯片TDC-GP2,同... 详细信息

超声波流量计是目前应用较广泛的一种流量计量设备,它以非接触方式进行流量计量,具有良好的应用前景。文中设计了一种基于超低功耗16位单片机MSP430微控制器的时差式超声波流量计,采用具有温度测量功能的高精度时间测量芯片TDC-GP2,同时还可以测出流体温度,自动补偿由于温度变化带来的误差,LCD液晶动态显示瞬时流量和总流量等参数。

关键词：超声波流量计 MSP430 TDC-GP2测量芯片时差法