检索结果-南通市图书馆

Prokaryotic Expression and Potential Application of the Truncated PCV-2 Capsid Protein

Virologica Sinica 2010年第2期25卷 86-97页

作者： zhong-zi lou Zhi-yong LI Gang WANG Jian-qiang LI Xi LAN Xue-rui LI Xiang-ping YIN Ji-xing LIU Si-dang LIU Animal Infectious Diseases Research Laboratory Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture Key Laboratory of Veterinary Public Health of Ministry of Agriculture Lanzhou 730046 China College of Veterinary Medicine Shandong Agricultural University Taian 271018 China

Three pairs of specific primers were designed to amplify the F2-1, F2-2 and XF2-2 truncated sequences of ORF2 which encodes the capsid protein of porcine circovirus type 2 （PCV-2）. The F2-1 sequence had most of the NLS region of ORF2, but the F2-2 and XF2-2 genes had the NLS region deleted. Truncated genes were subcloned into pET-32a（＋） vectors to construct recombinant fusion expression vectors. The vectors were then transformed into Rosetta（DE3） E. coli and expressed by induction of IPTG. Expressed proteins were detected by western blotting and ELISA. The protein with best immunoreactivity was confirmed and selected, then utilized to inoculate SPF rabbits to prepare polyclonal antibodies. The protein and prepared polyclonal antibody were utilized to detect sera samples against PCV-2 from Shandong province and PCV-2 particles in PK-15 cells. In our study, three recombinant fusion proteins were successfully obtained, and the molecular weights of fusion proteins were 35.9 kDa, 33.6 kDa and 38.6 kDa respectively detected by SDS-PAGE. All of the proteins showed positive reaction with anti-PCV-2 antisera, and His-XF2-2 showed better immunoreactivity than the others. The protein of His-XF2-2 was coated as antigen in ELISA to detect the seroprevalence of PCV-2 in certain districts of Shandong province, the seropositivity rate was 27.7 % （73/264）. Specific fluorescence and positive signals for PCV-2 could be detected in PK-15 cells inoculated with PCV-2 with the participation of prepared antibodies against His-XF2-2 in IFA and IPMA. Experimental results indicated that the truncated PCV-20RF2 gene containing most of the NLS region was successfully expressed in E. coli, and His-XF2-2 was demonstrated to have better immunoreactivity with anti-PCV-2 antisera than the other two fusion proteins. His-XF2-2 and prepared polyclonal antibodies against it had a satisfactory capability in detecting PCV-2 infection.

关键词： Porcine circovirus type 2 Capsid protein Fusion expression Polyclonal antibodies Virus detection

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cloning the Structure Genes and Expression the N Gene of Porcine Epidemic Diarrhea Virus DX

引用

Virologica Sinica 2009年第3期24卷 179-186页

作者： Jian-qiang LI Ji-xing LIU Xi LAN Jie CHENG Run WU zhong-zi lou Xiang-ping YIN Xue-rui LI Bao-yu LI Bin YANG Zhi-yong LI College of Veterinary Medicine Gansu Agricultural University Lanzhou 730070 China Key laboratory of Animal Virology of Ministry of Agriculture State Key Laboratory of Veterinary Etiological Biology Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou 730046China

The structure genes spike(S) ,nucleocapsid(N) ,membrane(M) ,small membrane(sM) of a porcine epidemic diarrhea virus(PEDV) strain DX isolated in Gansu province,North-west of China,were cloned,sequenced and compared with published sequences of PEDV strains. The nucleotide sequences encoding the entire S,sM,M and N genes open reading frame(ORF) of DX were 4 152,231,681 and 1 326 bases long respectively. There were transcription regulatory sequences(TRSs) upstream of the initiator ATG of the S,N and M genes. The amino acids sequences of S,M and N contained 30,3 and 7 potential asparagine(N) -linked glycosylation sites. Homologous analysis and phylogenetic trees showed that DX had the closest relationship with strains LJB/06,JS-2004-2Z and CH/HLJH/06 that were also isolated from China and indicated the prevalence of some PEDV isolates in China were widespread since the JS-2004-2Z strain originated from the south of the China,and LJB/06 and CH/HLJH/06 were isolated from northeast China. The N gene was cloned using two primers which contained Nco I and BamH I restriction enzyme sites and subcloned into expression vector pET30a. The recombinant plasmid was then transformed into *** Rossta. SDS-PAGE showed there was a protein of about 55kDa as expected and Western blot indicated the N protein had biological activity.

关键词： Cloning Structure genes N gene Expression PEDV

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Estimating the prevalence of Echinococcus in domestic dogs in highly endemic for echinococcosis

引用

Infectious Diseases of Poverty 2018年第1期7卷 761-769页

作者： Cong-Nuan Liu Yang-Yang Xu Angela M.Cadavid-Restrepo zhong-zi lou Hong-Bin Yan Li Li Bao-Quan Fu Darren J.Gray Archie A.Clements Tamsin S.Barnes Gail M.Williams Wan-zhong Jia Donald P.McManus Yu-Rong Yang State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Zoonoses of Agriculture Ministry/Lanzhou Veterinary Research Institute CAASLanzhou 730046People’s Republic of China The Human Pathology and Immunology Department Ningxia Medical UniversityYinchuanNingxia Hui Autonomous RegionPeople’s Republic of China Neurosurgery Department Tianjin Xiqing HospitalTianjinPeople’s Republic of China Molecular Parasitology Laboratory QIMR Berghofer Medical Research InstituteBrisbaneAustralia Research School of Population Health Australian National UniversityCanberraAustralia School of Population Health Infectious Disease Epidemiology UnitUniversity of QueenslandBrisbaneAustralia Queensland Alliance for Agriculture and Food Innovation University of QueenslandGattonAustralia

Background:Cystic echinococcosis(CE)and alveolar echinococcosis(AE)are highly endemic in Xiji County of Ningxia Hui Autonomous Region(NHAR)in China where the control campaign based on dog de-worming with praziquantel has been undertaken over preceding *** study is to determine the current prevalence of Echinococcus granulosus and *** in domestic dogs and monitor the echinococcosis transmission ***:Study villages were selected using landscape patterns(Geographic Information System,GIS)for Echinococcus transmission“hot spots”,combined with hospital records identifying risk areas for AE and CE.A survey of 750 domestic dogs,including copro-sampling and owner questionnaires,from 25 selected villages,was undertaken in 2012.A copro-multiplex PCR assay was used for the specific diagnosis of *** and *** in the *** analysis,using IBM SPSS Statistics,was undertaken,to compare the prevalence of the two Echinococcus *** dogs between four geographical areas of Xiji by theχ^(2)*** analysis of the combinations of outcomes from the questionnaire and copro-PCR assay data was carried out to determine the significant risk factors for dog ***:The highest de-worming rate of 84.0%was found in the northwest area of Xiji County,and significant differences(P0.05)in the prevalence of *** in dogs from the northwest,southwest,northeast,and southeast of Xiji,but there were significant differences(P<0.05)between dogs infected with *** from the four *** of the other independent variables was statistically ***:The results from this study indicate a high prevalenc

关键词： Domestic dog surveys Dog-copro-multiplex PCR assay Co-endemicity of Echinococcus granulosus and E.multilocularis Xiji County Ningxia hui autonomous region(NHAR) P.R.China

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Prevalence of Echinococcus granulosus and *** in domestic dogs in a Chinese community highly endemic for echinococcosis

Prevalence of Echinococcus granulosus and *** in domestic do...

引用

第五届全国人畜共患病学术研讨会

作者： Cong-Nuan Liu Yang-Yang Xu Angela M.Cadavid-Restrepo zhong-zi lou Hong-Bin Yan Li Li Bao-Quan Fu Darren J.Gray Archie A.Clements Tamsin S.Barnes Gail M.Williams Wan-zhong Jia Donald P.McManus Yu-Rong Yang State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Zoonoses of Agriculture Ministry/Lanzhou Veterinary Research Institute CAAS Neurosurgery Department Tianjin Xiqing Hospital Ningxia Medical Universities Molecular Parasitology Laboratory QIMR Berghofer Medical Research Institute Research School of Population Health Australian National University School of Population Health Infectious Disease Epidemiology UnitUniversity of Queensland Queensland Alliance for Agriculture and Food Innovation University of Queensland

Background:To undertake a survey to determine the infection prevalence of Echinococcus granulosus and *** in domestic dogs and to monitor the transmission dynamics of echinococcosis in Xiji County,Ningxia Hui Autonomous Region(NHAR),***,an area co-endemic for both human alveolar and cystic echinococcosis(AE and CE)where a control campaign,based on dog de-worming with praziquantel(PZQ),has been in operation over the past

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

面向可持续雨洪管理的低影响开发措施综合效益评估

引用

天津理工大学学报 2025年第1期41卷 126-133页

作者：訾妍钟炜天津理工大学管理学院

为满足低影响开发(low impact development, LID)组合在城市雨洪管理过程中良好的应用效果和经济效益，应用暴雨洪水管理模型(stormwater management model, SWMM)结果指标和项目全生命周期成本理论，构建不同LID组合方案下的环境-成本... 详细信息

为满足低影响开发(low impact development, LID)组合在城市雨洪管理过程中良好的应用效果和经济效益，应用暴雨洪水管理模型(stormwater management model, SWMM)结果指标和项目全生命周期成本理论，构建不同LID组合方案下的环境-成本效益综合评价系统，并通过数值模拟和数理分析相结合的方法评估各备选方案的综合效益。为验证理论模型的可行性，将9种不同布设比例的LID组合分别嵌入实际项目中进行模拟和评估。结果表明，综合效益最高的LID组合为“45%的绿色屋顶+15%的植草沟+30%的雨水花园+70%的透水铺装”,其径流总量削减率为52.83%、4类污染负荷削减率均为80%以上且每年消耗的总成本费用为22 416 350元。该评价体系为优化城市可持续雨洪管理提供新思路，同时为建设开发海绵城市提供方案指导。

关键词：低影响开发综合效益评估暴雨洪水管理模型可持续雨洪管理海绵城市

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Nickel Sulfide Modified NiCu Nanoalloy with Excellent Oxygen Evolution Reaction Properties Prepared through Electrospinning and Heat Treatment

引用

Journal of Wuhan University of Technology(Materials Science) 2024年第2期39卷 320-326页

作者：李涛凌帅 zhong Shujie lou Qiongyue Department of Materials Engineering School of Mechanical EngineeringChengdu UniversityChengdu 610106China

Ni^(2+)/Cu^(2+)/SO_(4)^(2-)/polyvinyl alcohol precursor fibers with uniform diameters were prepared through ***-based composite nanoalloys containing Ni,Cu,and S were prepared through heat treatment in an Ar *** experimental results show that the main components of the prepared nanoalloys are NiCu,Ni_(3)S_(2),Ni,and *** nanoalloys exhibit fine grain sizes about 200-500 nm,which can increase with increasing heat treatment *** test results show that the nickel sulfidemodified NiCu nanoalloy composites exhibit excellent oxygen evolution reaction properties,and the oxygen evolution reaction properties gradually improve with the increasing heat treatment *** sample prepared at 1 000℃ for 40 min show a low overpotential of 423 mV and a small Tafel slope of 134 mV·dec^(-1) at a current density of 10 mA·cm^(-2).

关键词： NiCu nanoalloy Ni_(3)S_(2) structure transformation oxygen evolution reaction

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The asymptotic relation between the first crossing point and the last exit time of Gaussian order statistics sequences

引用

Applied Mathematics(A Journal of Chinese Universities) 2023年第4期38卷 545-561页

作者： NING zi-jun TAN zhong-quan College of Data Science Jiaxing UniversityJiaxing 314001China

In this paper,we study the asymptotic relation between the first crossing point and the last exit time for Gaussian order statistics which are generated by stationary weakly and strongly dependent Gaussian *** is shown that the first crossing point and the last exit time are asymptotically independent and dependent for weakly and strongly dependent cases,*** asymptotic relations between the first crossing point and the last exit time for stationary weakly and strongly dependent Gaussian sequences are also obtained.

关键词： rst crossing point last exit time stationary Gaussian sequences Gaussian order statistic se-quences

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Memory,Time and Technique Aspects of Density Matrix Renormalization Group Method

引用

Communications in Theoretical Physics 2001年第11期36卷 635-640页

作者： QIN Shao-Jin lou Ji-zhong Department of Physics Kyushu University6-10-1 HakozakiHigashi-kuFukuoka 812-8581Japan Institute of Theoretical Physics P.O.Box 2735Beijing 100080China Department of PhysicsUniversity of NevadaLas VegasNV 89154USA

We present the memory size,computational time,and technique aspects of density matrix renormalization group (DMRG) *** show how to estimate the memory size and computational time before starting a large scale DMRG *** propose an implementation of the Hamiltonian wavefunction multiplication and a wavefunction initialization in DMRG with block matrix data ***-dimensional Heisenberg model is used to illustrate our study.

关键词： density matrix renormalization group,one-dimensional antiferromagnetic Heisenberg model

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Multimodal imaging findings in normal-appearing white matter of leucoaraiosis: a review

引用

Stroke & Vascular Neurology 2016年第2期1卷 59-63页

作者： Genlong zhong Min lou Department of Neurology The Second Affiliated Hospital of Zhejiang UniversitySchool of MedicineHangzhouZhenjiangChina

Leucoaraiosis(LA),also referred to as white matter hyperintensities(WMHs),are usually seen as patchy or confluent hyperintense areas on T2-weighted or fluid-attenuated inversion recovery MRI in the *** is often asymptomatic in its early stages,yet its persistent evolution to more advanced stages may lead to substantial neurological dysfunction including dementia,stroke and *** its clinical significance,the pathogenic mechanisms underlying LA development are *** patients with LA,the pathophysiological changes in white matter(WM)are suggested to be continuous from WMHs to its neighbourhood‘normal-appearing white matter(NAWM)’on conventional MRI *** imaging studies revealed that the so-called‘NAWM’was actually abnormal with regard to underlying haemodynamic and microstructural *** the basis of positron emission tomography CT,xenon-CT,perfusion MRI,etc,the cerebral blood flow of NAWM was found to be significantly reduced in patients with LA,compared with healthy ***,the integrity of microstructures and blood–brain barrier in NAWM was also demonstrated to be impaired with diffusion tensor imaging and dynamic contrastenhanced MRI studies,*** addition,the integrity of NAWM correlated much stronger with cognitive performance than did WMHs *** is reasonable to assume that the subtle injury of NAWM would be more reversible than WMHs ***,multimodal imaging modalities could be appropriately applied to future interventional studies targeting at early pathophysiological changes of *** this paper,we summarise current knowledge about NAWM of LA mainly acquired from multimodal imaging studies in vivo,and attempt to give options for future work.

关键词： impaired white finding

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Decomposing Triples of Z_(p^n) and Z_(3p^n) into Cyclic Designs

引用

Acta Mathematica Sinica,English Series 2013年第11期29卷 2111-2128页

作者： zi Hong TIAN Rui zhong WEI College of Mathematics and Information Science Hebei Normal University Department of Computer Science Lakehead University

In this paper, we give some decompositions of triples of Zp^n or Z3p^n into cyclic triple systems. New constructions of difference families are given. Some infinite classes of simple cyclic triple systems are obtained... 详细信息

关键词： Decomposition cyclic designs triple systems simple designs difference families

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：