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A 1-bp deletion in the MC04g1399 is highly associated with failure to produce fruit wart in bitter gourd

Horticultural Plant Journal 2024年第1期10卷 171-180页

作者： Jia Liu Junjie Cui Jichi Dong Jian zhong Chunfeng zhong Fanchong yuan Wendong Guan Fang Hu Jiaowen Cheng Kailin Hu College of Horticulture South China Agricultural UniversityGuangzhouGuangdong 510642China Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China) Ministry of Agriculture and Rural AffairsGuangzhouGuangdong 510642China Guangdong Vegetables Engineering Research Center GuangzhouGuangdong 510642China Department of Horticulture Foshan UniversityFoshanGuangdong 528225China Henry Fok School of Biology and Agricultural Shaoguan UniversityShaoguanGuangdong 512023China

Fruit wart is an important appearance trait influencing consumer preferences of bitter gourd(Momordica charantia L.).The molecular genetic mechanisms underlying fruit wart formation in bitter gourd are largely *** this study,genetic analysis based on four generations showed that fruit wart formation in bitter gourd was controlled by a single dominant locus named as *** Fwa locus was initially mapped into a 4.82 Mb region on pseudochromosome 4 by BSA-seq analysis and subsequently narrowed down to a 286.30 kb region by linkage analysis.A large F2population consisting of 2360 individuals was used to screen recombinants,and the Fwa locus was finally fine mapped into a 22.70 kb region harboring four protein-coding genes through recombination ***04g1399,encoding an epidermal patterning factor 2-like protein,was proposed as the best candidate gene for Fwa via sequence variation and expression *** addition,a 1-bp insertion and deletion(InDel)variation within MC04g1399 was converted to a cleaved amplified polymorphic sequence(CAPS)marker that could precisely distinguish between the warty and non-warty types with an accuracy rate of 100%among a wide panel of 126 bitter gourd germplasm *** results not only provide a scientific basis for deciphering the molecular mechanisms underlying fruit wart formation but also provide a powerful tool for efficient genetic improvement of fruit wart via marker-assisted selection.

关键词： Bitter gourd Fruit wart Bulk segregant analysis Fine-mapping Candidate gene

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A Dielectric Ink Combining Acrylate and Cyanate Moieties for Inkjet 3D Printing with Good Thermal Stability

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Chinese Journal of Polymer Science 2024年第6期42卷 766-774页

作者： Yi-Xuan Li Liang-Ying Wu Yan Yang Yao-zhong lu zhong-Ying Ji Yu-Xiong Guo Xiao-Long Wang Qiao-Sheng Pu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical EngineeringLanzhou UniversityLanzhou730000China State Key Laboratory of Solid lubrication Lanzhou Institute of Chemical PhysicsLanzhou730000China Lanzhou Traditional Chinese Medicine Hospital of Orthopedics and Traumatology Lanzhou730000China Shandong Laboratory of Advanced Materials and Green Manufacturing at Yantai Yantai Zhongke Research Institute of Advanced Materials and Green Chemical EngineeringYantai264006China

Inkjet 3D printing has potential in the additive manufacturing of electronic circuits and ***,inks that can be used for printing layers with T5%>300℃ or hardness>200 MPa have been rarely *** ester(CE)polymers have excellent thermal stability,high strength,and low shrinkage compared to other common dielectric inks for inkjet 3D printing,but cannot be quickly shaped by ultraviolet(UV)irradiation or thermal *** CEs with UV-curable monomers may be a possible way to accelerate crosslinking,but there are challenges from the adverse effects of the dilution of both *** this study,dielectric inks with acrylate and cyanate moieties were *** low viscosity and surface tension of the CE precursor(Bisphenol E cyanate ester)were combined with photocurable acrylate diluent monomers and cross-linker to realize an ink suitable for inkjet 3D *** internal dual three-dimensional cross-linked network structure resin was prepared by a combination of photocuring and thermal curing with T5%up to 326.69℃,hardness up to 431.84 MPa,dielectric constant of 2.70 at 8 GHz,and shrinkage of 1.64%.The developed dielectric inks can be applied to the 3D printing of printed circuit boards and other electronic devices that require dielectric properties.

关键词： Inkjet printing Cyanate ester resin 3D doubly cross-linked network Good thermal stability

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Molecular insights into self-incompatibility systems:From evolution to breeding

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Plant Communications 2024年第2期5卷 23-45页

作者： Diyang Zhang yuan-yuan Li Xuewei Zhao Cuili Zhang Ding-Kun Liu Siren Lan Weilun Yin zhong-Jian Liu Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization Fujian Agriculture and Forestry UniversityFuzhou 350002China College of Forestry Fujian Agriculture and Forestry UniversityFuzhou 350002China College of Biological Sciences and Technology Beijing Forestry UniversityBeijing 100083China

Plants have evolved diverse self-incompatibility(SI)systems for *** Darwin’s time,consid-erable progress has been made toward elucidating this unrivaled reproductive *** advances in interdisciplinary studies and applications of biotechnology have given rise to major break-throughs in understanding the molecular pathways that lead to SI,particularly the strikingly different SI mechanisms that operate in Solanaceae,Papaveraceae,Brassicaceae,and *** best-un-derstood SI systems,together with discoveries in other"nonmodel"SI taxa such as Poaceae,suggest a complex evolutionary trajectory of SI,with multiple independent origins and frequent and irreversible *** exploration of self-/nonself-discrimination signaling cascades has revealed a compre-hensive catalog of male and female identity genes and modifier factors that control *** also enable the characterization,validation,and manipulation of SI-related factors for crop improvement,helping to address the challenges associated with development of inbred ***,we review current knowledge about the evolution of SI systems,summarize key achievements in the molecular basis of pol-len‒pistil interactions,discuss potential prospects for breeding of SI crops,and raise several unresolved questions that require further investigation.

关键词： self-incompatibility S-RNase evolution genome editing crop improvement

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Highly efficient expression of human extracellular superoxide dismutase(rhEcSOD)with ultraviolet-B-induced damage-resistance activity in transgenic silkworm cocoons

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Insect Science 2024年第4期31卷 1150-1164页

作者： Feng Wang Ri-yuan Wang De-Bin zhong Ping Zhao Qing-You Xia Integrative Science Center of Germplasm Creation in Western China(CHONGQING)Science City Biological Science Research CenterSouthwest UniversityChongqingChina Century Legend Biotechnology Research Institute(Chongqing)Co. Ltd.ChongqingChina

Extracellular superoxide dismutase(EcSOD)protects tissues from oxidative stress,and thus is considered as a therapeutic agent for many diseases such as atherosclerosis,hypertension,and ***,cost-effective production of bioactive recombinant human EcSOD(rhEcSOD)remains a ***,we developed an efficient strategy for producing active rhEcSOD by transgenic *** was successfully synthesized as homodimers and homotetramers in the middle silk gland and spun into the cocoons with a concentration of 9.48±0.21 mg/*** of rhEcSOD from the cocoons could be conveniently achieved with a purity of 99.50%and a yield of 3.5±0.5 mg/***,N-glycosylation at the only site of N89 in rhEcSOD with 10 types were *** purified rhEcSOD gained the potent enzymatic activity of 4162±293 U/mg after Cu/Zn ions *** importantly,rhEcSOD was capable of penetrating and accumulating in the nuclei of cells to maintain cell morphology and attenuate ultraviolet B-induced cell apoptosis by eliminating reactive oxygen species and inhibiting the C-Jun N-terminal kinase signaling *** results demonstrated that the transgenic silkworm could successfully produce rhEcSOD with enzymatic and biological activities for biomedical applications.

关键词： antioxidant human extracellular superoxide dismutase recombinant expression silkworm silk gland

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On-surface photo-induced dechlorination

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Chinese Chemical Letters 2024年第9期35卷 198-201页

作者： Yu He Hao Jiang Shaoxuan yuan Jiayi lu Qiang Sun Materials Genome Institute Shanghai UniversityShanghai 200444China

On-surface Ullmann-type reaction,or the dehalogenated coupling,is arguably the most pivotal reaction in on-surface synthesis for the fabrications of carbon ***,the vast majority of works rely on activating the C-Br bond of aryl bromide which has a moderate bond dissociation *** C-Cl bond of aryl chloride has a higher dissociation energy and requires much higher thermal energy to break the *** this study,we have explored the on-surface photo-induced dechlorination and achieved the activation of three distinct aryl chlorines on the Au(111)surface with mild *** work enriches our understanding of on-surface photo-induced reactions and highlights the potential of photochemistry in realizing unconventional reactions.

关键词： On-surface synthesis Scanning probe microscopy On-surface photochemistry Dechlorination reaction

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Achieving a superior Na storage performance of Fe-based Prussian blue cathode by coating perylene tetracarboxylic dianhydride amine

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Carbon Energy 2024年第5期6卷 53-64页

作者： Xin-yuan Fu lu-lu Zhang Zhao-Yao Chen Yunkai Xu Junxiu Wu Cheng-Cheng Wang Xiao-Kai Ding Xue-Lin Yang Jun lu Hubei Provincial Collaborative Innovation Center for New Energy Microgrid College of Electrical Engineering&New EnergyChina Three Gorges UniversityYichangHubeiChina College of Materials and Chemical Engineering China Three Gorges UniversityYichangHubeiChina College of Chemical and Biological Engineering Zhejiang UniversityHangzhouChina

Fe-based Prussian blue(Fe-PB)cathode material shows great application potential in sodium(Na)-ion batteries due to its high theoretical capacity,long cycle life,low cost,and simple preparation ***,the crystalline water and vacancies of Fe-PB lattice,the low electrical conductivity,and the dissolution of metal ions lead to limited capacity and poor cycling *** this work,a perylene tetracarboxylic dianhydride amine(PTCDA)coating layer is successfully fabricated on the surface of Fe-PB by a liquid-phase *** aminated PTCDA(PTCA)coating not only increases the specific surface area and electronic conductivity but also effectively reduces the crystalline water and vacancies,which avoids the erosion of Fe-PB by ***,the PTCA layer reduces the charge transfer resistance,enhances the Na-ion diffusion coefficient,and improves the structure *** PTCA-coated Fe-PB exhibits superior Na storage performance with a first discharge capacity of 145.2 mAh g^(−1) at 100 mA g^(−1).Long cycling tests exhibit minimal capacity decay of 0.027%per cycle over 1000 cycles at 1 A g^(−1).Therefore,this PTCA coating strategy has shown promising competence in enhancing the electrochemical performance of Fe-PB,which can potentially serve as a universal electrode coating strategy for Na-ion batteries.

关键词： cathode material coating Fe-based Prussian blue Na-ion batteries perylene tetracarboxylic dianhydride amine

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Development and parentage analysis of SNP markers for Chestnut-vented Nuthatch(Sitta nagaensis)based on ddRAD-seq data

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Avian Research 2024年第2期15卷 271-278页

作者： Qingmiao yuan Xi lu Ruixin Mo Xianyin Xu Xu luo Yubao Duan Key Laboratory for Conserving Wildlife with Small Populations in Yunnan Southwest Forestry UniversityKunming 650224China Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China Ministry of EducationSouthwest Forestry UniversityKunming 650224China Key Laboratory of Forest Disaster Warning and Control of Yunnan Province Southwest Forestry UniversityKunming 650224China College of Forestry Southwest Forestry UniversityKunming 650224China Administration of Zixi Mountain Provincial Nature Reserve Chuxiong 675008China

Extra-pair paternity(EPP)is commonly found in socially monogamous birds,especially in small passerine birds,and there are interspecific and intraspecific variations in the extent of *** Chestnut-vented Nuthatch(Sitta nagaensis)is a socially monogamous passerine bird,and verifying whether this species has EPP relies on parentage *** is not known to have *** this study,we developed SNP markers of this species that are informative for parentage analysis from double digest restriction site-associated DNA sequencing(ddRAD-seq)data.A panel consisting of 50 SNP markers,with a mean heterozygosity of 0.343,was used to resolve 95% of nestlings to *** combined exclusion probabilities for the first parent and second parent were 0.991 and 0.9999,*** panel of SNP markers is a powerful tool for parentage assignments in *** addition,we found that three offspring(7.9%)from three nests(23.1%)were the result of extra-pair fertilization out of 38 offspring in 13 *** study provided information on parentage analysis that has not been reported before in *** also supplemented the understudied EPP behavior of birds in Asia,contributing to a general understanding of the EPP behaviors of birds.

关键词： Chestnut-vented Nuthatch ddRAD sequencing Extra-pair paternity Parentage analysis SNPs

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Geobacter sulfurreducens promoted the biosynthesis of reduced graphene oxide and coupled it for nitrobenzene reduction

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Journal of Environmental Sciences 2024年第4期138卷 458-469页

作者： Shoujuan Zhang Qi Liu Linrui zhong Jianhong Jiang Xiaozhe luo Xingxin Hu Qian Liu Yue lu College of Environmental Science and Engineering Hunan UniversityChangsha 410082China Shenzhen Research Institute Hunan UniversityShenzhen 510082China Key Laboratory of Environment Biology and Pollution Control Hunan UniversityMinistry of EducationChangsha 410082China China Machinery International Engineering Design&Research Institute Co. LtdChangsha 410007China Hunan Engineering Research Center for Water Treatment Process&Equipment Changsha 410007China

In order to explore an efficient and green method to deal with nitrobenzene(NB)pollutant,reduced graphene oxide(r GO)as an electron shuttle was applied to enhance the extracellular electron transfer(EET)process of Geobacter sulfurreducens,which was a typical electrochemically active bacteria(EAB).In this study,r GO biosynthesis was achieved via the reduction of graphene oxide(GO)by *** PCA within 3 ***,the r GOPCA combining system completely reduced 50-200μmol/L of NB to aniline as end product within one *** characterization revealed that PCA cells were partly wrapped by rGO,and therefore the distance of electron transfer between strain PCA and r GO material was ***,the ID/IGof GO,r GO,and r GO-PCA combining system were 0.990,1.293 and 1.31,***,highest currents were observed in r GO-PCA-NB as 12.950μA/-12.560μA at -408 m V/156 m V,attributing to the faster electron transfer efficiency in EET ***,the NB reduction was mainly due to:(I)direct EET process from *** PCA to NB;(II)r GO served as electron shuttle and accelerated electron transfer to NB,which was the main degradation ***,the biosynthesis of r GO via GO reduction by Geobacter promoted the NB removal process,which provided a facile strategy to alleviate the problematic nitroaromatic pollution in the environment.

关键词： Reduced graphene oxide Geobacter Nitrobenzene Removal Extracellular electron transfer

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Self-polarized RGB device realized by semipolar micro-LEDs and perovskite-in-polymer films for backlight applications

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Opto-Electronic Advances 2024年第3期7卷 35-49页

作者： Tingwei lu Yue Lin Tianqi Zhang Yue Huang Xiaotong Fan Shouqiang Lai Yijun lu Hao-Chung Kuo zhong Chen Tingzhu Wu Rong Zhang School of Electronic Science and Engineering Fujian Engineering Research Center for Solid-State LightingXiamen UniversityXiamen 361005China Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province(IKKEM) Xiamen 361005China Department of Photonics and Graduate Institute of Electro-Optical Engineering College of Electrical and Computer EngineeringYang Ming Chiao Tung UniversityHsinchu 30010China Semiconductor Research Center Hon Hai Research InstituteTaipei 11492China.

In backlighting systems for liquid crystal displays,conventional red,green,and blue(RGB)light sources that lack polarization properties can result in a significant optical loss of up to 50%when passing through a *** address this inefficiency and optimize energy utilization,this study presents a high-performance device designed for RGB polarized *** device employs an array of semipolar blueμLEDs with inherent polarization capabilities,coupled with mechanically stretched films of green-emitting CsPbBr3 nanorods and red-emitting CsPbI3-Cs4PbI6 hybrid *** CsPbBr3 nanorods in the polymer film offer intrinsic polarization emission,while the aligned-wire structures formed by the stable CsPbI3-Cs4PbI6 hybrid nanocrystals contribute to substantial anisotropic emissions,due to their high dielectric *** resulting device achieved RGB polarization degrees of 0.26,0.48,and 0.38,respectively,and exhibited a broad color gamut,reaching 137.2%of the NTSC standard and 102.5%of the Rec.2020 *** compared to a device utilizing c-plane LEDs for excitation,the current approach increased the intensity of light transmitted through the polarizer by 73.6%.This novel fabrication approach for polarized devices containing RGB components holds considerable promise for advancing next-generation display technologies.

关键词： halide perovskite light-emitting-diodes polarized emission nanocrystals stability

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Impact of early refined nursing program on prognosis of middleaged and elderly patients with cognitive dysfunction combined with cerebral infarction

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World Journal of Psychiatry 2024年第7期14卷 1034-1042页

作者： Hui-Lian Xiong Zhi-Xin Li Xin lu Yan-Hua lu Ping zhong Ganzhou City Central Blood Station Ganzhou 341000Jiangxi ProvinceChina Department of Rehabilitation Medicine Ganzhou People's HospitalGanzhou 341000Jiangxi ProvinceChina

BACKGROUND Cerebral infarction is a local or extensive necrosis of brain ***,the corresponding neurological deficits *** incidence of cerebrovascular diseases in China is increasing *** the onset of cerebrovascular disease,the most common sequelae include movement disorders,language disorders,and cognitive *** To investigate the effect of early refined nursing program on the prognosis of middle-aged and elderly patients with cerebral infarction combined with cognitive *** A retrospective study was conducted to divide 60 patients with cerebral infarction and cognitive impairment into an experimental group(n=32)and a control group(n=28).The experimental group received early intensive care every day,and the control group received daily routine *** scores of the Mini-Mental State Examination(MMSE)and the Trail Making Test(TMT),as well as the latency and amplitude of the event-related potential P300,were used as main indicators to evaluate changes in cognitive function,and changes in BDNF,TGF-β,and GDNF expression were used as secondary *** Both groups experienced notable enhancements in MMSE scores,with the experi-mental group demonstrating higher scores than the control group(experimental:28.75±2.31;control:25.84±2.87).Moreover,reductions in TMT-A and TMT-B scores were observed in both groups(experimental:TMT-A 52.36±6.18,TMT-B 98.47±10.23;control:TMT-A 61.48±7.92,TMT-B 112.63±12.55),with the experimental group displaying lower scores.P300 Latency decreased(experimental:270.63 ms±14.28 ms;control:285.72 ms±16.45 ms),while amplitude increased(experimental:7.82μV±1.05μV;control:6.35μV±0.98μV)significantly in both groups,with superior outcomes in the experimental ***,the levels of the growth factors BDNF,TGF-β1,and GDNF surged(experimental:BDNF 48.37 ng/mL±5.62 ng/mL,TGF-β152.14 pg/mL±4.28 pg/mL,GDNF 34.76 ng/mL±3.89 ng/mL;control:BDNF 42.58 ng/mL±4.73 ng/mL,TGF-β146.23 pg/m

关键词： Early refined nursing program Cerebral infraction Cognitive impairment Psychiatry Trajectory test

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