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Endothelial glycocalyx as a potential theriapeutic target in organ injuries

Chinese Medical Journal 2019年第8期132卷 963-975页

作者： Rui-Na Cao Li Tang Zhong-Yuan Xia Rui xia Department of Anesthesiology The First Affiliated Hospital of Yangtze UniversityJingzhouHubei 434000China Department of Anesthesiology Renmin Hospital of Wuhan UniversityWuhanHubei 430061China

Objective:The endothelial glycocalyx(eGC)is a dynamic and multicomponent layer of macromolecules found at the surface of vascular endothelium,which is largely underappreciated.It has recently been recognized that eGC is a major regulator of endothelial function and may have therapeutic value in organ injuries.This study aimed to explore the role of the eGC in various pathologic and physiologic conditions,by reviewing the basic research findings pertaining to the detection of the eGC and its clinical significance.We also explored different pharmacologic agents used to protect and rebuild the eGC.Data sources:An in-depth search was performed in the PubMed database,focusing on research published after 2003 with keywords including eGC,permeability,glycocalyx and injuries,and glycocalyx protection.Study selection:Several authoritative reviews and original studies were identified and reviewed to summarize the characteristics of the eGC under physiologic and pathologic conditions as well as the detection and protection of the eGC.Results:The eGC degradation is closely associated with pathophysiologic changes such as vascular permeability,edema formation,mechanotransduction,and clotting cascade,together with neutrophil and platelet adhesion in diverse injury and disease states including inflammation(sepsis and trauma),ischemia-reperfusion injury,shock,hypervolemia,hypertension,hyperglycemia,and high Na+as well as diabetes and atherosclerosis.Therapeutic strategies for protecting and rebuilding the eGC should be explored through experimental test and clinical verifications.Conclusions:Disturbance of the eGC usually occurs at early stages of various clinical pathophysiologies which can be partly prevented and reversed by protecting and restoring the eGC.The eGC seems to be a promising diagnostic biomarker and therapeutic target in clinical settings.

关键词： Endothelial glycocalyx Permeability Injuries Glycocalyx protection

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Puerarin ameliorates allodynia and hyperalgesia in rats with peripheral nerve injury

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Neural Regeneration Research 2018年第7期13卷 1263-1268页

作者： Heng-Tao Xie Zhong-Yuan Xia xia Pan Bo Zhao Zhi-Gang Liu Department of Anesthesiology Renmin Hospital of Wuhan University

Puerarin is a major active ingredient of the traditional Chinese plant medicine,Radix Puerariae,and commonly used in the treatment of myocardial and cerebral ischemia.However,the effects of puerarin on neuropathic pain are still unclear.In this study,a neuropathic pain animal model was created by partial sciatic nerve ligation.Puerarin（30 or 60 mg/kg） was intraperitoneally injected once a day for 7 days.Mechanical allodynia and thermal hyperalgesia were examined at 1 day after model establishment.Mechanical threshold and paw withdrawal latency markedly increased in a dose-dependent manner in puerarin-treated rats,especially at 7 days after model establishment.At 7 days after model establishment,quantitative real-time reverse transcriptase-polymerase chain reaction results showed that puerarin administration reversed m RNA expression of transient receptor potential vanilloid 1（Trpv1） and transient receptor potential ankyrin 1（Trpa1） in a dose-dependent manner in dorsal root ganglion neurons after peripheral nerve injury.These results suggest that puerarin dose-dependently ameliorates neuropathic pain by suppressing Trpv1 and Trpa1 up-regulation in dorsal root ganglion of neuropathic pain rats.

关键词： nerve regeneration puerarin Radix Puerariae traditional Chinese medicine Trpv1 Trpa1 dorsal root ganglion peripheral nerve injury neuropathic pain mechanical allodynia thermal hyperalgesia neural regeneration

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Exendin-4 attenuates pain-induced cognitive impairment by alleviating hippocampal neuroinflammation in a rat model of spinal nerve ligation

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Neural Regeneration Research 2020年第7期15卷 1333-1339页

作者： Shan-Shan Cui xiao-Bo Feng Bing-Hong Zhang Zhong-Yuan Xia Li-Ying Zhan Department of Anesthesiology Renmin Hospital of Wuhan UniversityWuhanHubei ProvinceChina Department of Anesthesiology Zhongnan Hospital of Wuhan UniversityWuhanHubei ProvinceChina

Glucagon-like peptide-1 receptor has anti-apoptotic,anti-inflammatory,and neuroprotective effects.It is now recognized that the occurrence and development of chronic pain are strongly associated with anti-inflammatory responses;however,it is not clear whether glucagon-like peptide-1 receptor regulates chronic pain via anti-inflammatory mechanisms.We explored the effects of glucagon-like peptide-1 receptor on nociception,cognition,and neuroinflammation in chronic pain.A rat model of chronic pain was established using left L5 spinal nerve ligation.The glucagon-like peptide-1 receptor agonist exendin-4 was intrathecally injected into rats from 10 to 21 days after spinal nerve ligation.Electrophysiological examinations showed that,after treatment with exendin-4,paw withdrawal frequency of the left limb was significantly reduced,and pain was relieved.In addition,in the Morris water maze test,escape latency increased and the time to reach the platform decreased following exendin-4 treatment.Immunohistochemical staining and western blot assays revealed an increase in the numbers of activated microglia and astrocytes in the dentate gyrus of rat hippocampus,as well as an increase in the expression of tumor necrosis factor alpha,interleukin 1 beta,and interleukin 6.All of these effects could be reversed by exendin-4 treatment.These findings suggest that exendin-4 can alleviate pain-induced neuroinflammatory responses and promote the recovery of cognitive function via the glucagon-like peptide-1 receptor pathway.All experimental procedures and protocols were approved by the Experimental Animal Ethics Committee of Renmin Hospital of Wuhan University of China(approval No.WDRM 20171214)on September 22,2017.

关键词： astrocyte chronic pain cognitive impairment exendin-4 hippocampal dentate gyrus microglia Morris water maze neuroinflammation spinal nerve ligation

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Advanced Pt hollow nanospheres/rubrene nanoleaves coupled with M-shaped DNA walker for ultrasensitive electrochemiluminescence bioassay

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Chinese Chemical Letters 2023年第6期34卷 247-251页

作者： Yumeng Song Xiuli Tao Wenbin Liang xia zhong Ruo yuan Ying Zhuo Key Laboratory of Luminescence Analysis and Molecular Sensing Ministry of EducationCollege of Chemistry and Chemical EngineeringSouthwest UniversityChongqing 400715China

Herein,an intense electrochemiluminescence(ECL)was achieved based on Pt hollow nanospheres/rubrene nanoleaves(Pt HNSs/Rub NLs)without the addition of any coreactant,which was employed for ultrasensitive detection of carcinoembryonic antigen(CEA)coupled with an M-shaped DNA walker(M-DNA walker)as signal switch.Specifically,in comparison with platinum nanoparticles(Pt NPs),Pt HNSs revealed excellent catalytic performance and pore confinement-enhanced ECL,which could significantly amplify ECL intensity of Rub NLs/dissolved O_(2)(DO)binary system.Then,the tracks and M-DNA walker were confined on the Pt HNSs simultaneously to promote the reaction efficiency,whose M-structure boosted the interaction sites between walking strands and tracks and reduced the rigidity of their recognition.Once the CEA approached the sensing interface,the M-DNA walker was activated based on highly specific aptamer recognition to recover ECL intensity with the assistance of exonucleaseⅢ(ExoⅢ).As proof of concept,the“on-off-on”switch aptasensor was constructed for CEA detection with a low detection limit of 0.20 fg/m L.The principle of the constructed ECL aptasensor also enables a universal platform for sensitive detection of other tumor markers.

关键词： Platinum hollow nanospheres Electrochemiluminescence aptasensor M-shaped DNA walker On-off-on switch Carcinoembryonic antigen

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Fast topological pumping for the generation of large-scale Greenberger–Horne–Zeilinger states in a superconducting circuit

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Frontiers of physics 2022年第6期17卷 143-161页

作者： Jin-Xuan Han Jin-Lei Wu zhong-Hui yuan Yan xia Yong-yuan Jiang Jie Song School of Physics Harbin Institute of TechnologyHarbin 150001China Department of Optoelectronics Science Harbin Institute of TechnologyWeihai 264209China Department of Physics Fuzhou UniversityFuzhou 350002China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006China Key Laboratory of Micro-Nano Optoelectronic Information System Ministry of Industry and Information TechnologyHarbin 150001China Key Laboratory of Micro-Optics and Photonic Technology of Heilongjiang Province Harbin Institute of TechnologyHarbin 150001China

Topological pumping of edge states in the finite lattice with nontrivial topological phases provides a powerful means for robust excitation transfer,requiring extremely slow evolution to follow an adiabatic transfer.Here,we propose fast topological pumping via edge channels to generate large-scale Greenberger–Horne–Zeilinger(GHZ)states in a topological superconducting circuit with a sped-up evolution process.The scheme indicates a conceptual way of designing fast topological pumping related to the instantaneous energy spectrum characteristics rather than relying on the shortcuts to adiabaticity.Based on fast topological pumping,large-scale GHZ states show greater robustness against on-site potential defects,the fluctuation of couplings and losses of the system in comparison with the conventional adiabatic topological pumping.With experimentally feasible qutrit-resonator coupling strengths and moderate decay rates of qutrits and resonators,fast topological pumping drastically improves the scalability of GHZ states with a high fidelity.Our work opens up prospects for the realization of large-scale GHZ states based on fast topological pumping in the superconducting quantum circuit system,which provides potential applications of topological matters in quantum information processing.

关键词： topological pumping superconducting ciruit large-scale Greenberger-Horne-Zeilinger states

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Stretching Elasticity and Flexibility of Single Polyformaldehyde Chain

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Chinese Journal of Polymer Science 2022年第4期40卷 333-337页

作者： Jin-xia Yang Hu-Jun Qian Zheng Gong zhong-yuan Lu Shu-Xun Cui Key Laboratory of Advanced Technologies of Materials(Ministry of Education) Southwest Jiaotong UniversityChengdu 610031China State Key Laboratory of Supramolecular Structure and Materials Institute of Theoretical ChemistryJilin UniversityChangchun 130023China School of Life Science and Engineering Southwest Jiaotong UniversityChengdu 610031China

In this work,the single-chain elasticity of polyformaldehyde(POM)is studied,for the first time,by employing atomic force microscopy(AFM)-based single molecule force spectroscopy(SMFS).We find that the single-chain elasticity of POM in a nonpolar organic solvent(nonane)can be described well by a theoretical model(QM-FRC model),when the rotating unit length is 0.144 nm(C―O bond length).After comparison,POM is more flexible than polystyrene(a typical polymer with C―C backbone)at the single-chain level,which is reasonable since the C―O bond has a lower rotation barrier than C―C bond.This result indicates that the flexibility of a polymer chain can be tuned by the C―O bond proportion in backbone,which casts new light on the rational design of new synthetic polymers in the future.

关键词： Polyformaldehyde Single molecule force spectroscopy Inherent elasticity Flexibility QM-FRC model

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Shenmai Injection Attenuates Myocardial Ischemia/Reperfusion Injury by Targeting Nrf2/GPX4 Signalling-Mediated Ferroptosis

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Chinese Journal of Integrative Medicine 2022年第11期28卷 983-991页

作者： MEI Sheng-lan xia zhong-yuan QIU Zhen JIA Yi-fan ZHOU Jin-jian ZHOU Bin Department of Anaesthesiology Renmin Hospital of Wuhan UniversityWuhan(430060)China

Objective:To examine the effect of Shenmai Injection(SMJ)on ferroptosis during myocardial ischemia reperfusion(I/R)injury in rats and the underlying mechanism.Methods:A total of 120SPF-grade adult male SD rats,weighing 220–250 g were randomly divided into different groups according to a random number table.Myocardial I/R model was established by occluding the left anterior descending artery for 30 min followed by 120 min of reperfusion.SMJ was injected intraperitoneally at the onset of 120 min of reperfusion,and erastin(an agonist of ferroptosis),ferrostatin-1(Fer-1,an inhibitor of ferroptosis)and ML385(an inhibitor of nuclear factor erythroid-2 related factor 2(Nrf2))were administered intraperitoneally separately 30 min before myocardial ischemia as different pretreatments.Cardiac function before ischemia,after ischemia and after reperfusion was analysed.Pathological changes in the myocardium and the ultrastructure of cardiomyocytes were observed,and the myocardial infarction area was measured.Additionally,the concentration of Fein heart tissues and the levels of creatine kinase-MB(CK-MB),troponin I(cTnI),malondialdehyde(MDA)and superoxide dismutase(SOD)in serum were measured using assay kits,and the expressions of Nrf2,glutathione peroxidase 4(GPX4)and acyl-CoA synthetase long-chain family member 4(ACSL4)were examined by Western blot.Results:Compared with the sham group,I/R significantly injured heart tissues,as evidenced by the disordered,ruptured and oedematous myocardial fibres;the increases in infarct size,serum CK-MB,cTnI and MDA levels,and myocardial Feconcentrations;and the decreases in SOD activity(P<0.05).These results were accompanied by ultrastructural alterations to the mitochondria,increased expression of ACSL4 and inhibited the activation of Nrf2/GPX4 signalling(P<0.05).Compared with the I/R group,pretreatment with 9 mL/kg SMJ and 2 mg/kg Fer-1 significantly reduced myocardial I/R injury,Feconcentrations and ACSL4 expression and attenuated mitochondrial impairment,while 14 mg/kg erastin exacerbate

关键词： ferroptosis Shenmai Injection myocardial ischemia reperfusion injury pretreatment

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"Light-on"Colorimetric Assay for Ascorbic Acid Detection via Boosting the Peroxidase-like Activity of Fe-MIL-88

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Journal of Analysis and Testing 2022年第1期6卷 67-75页

作者： Chuan-xia Chen Cheng-hui Zhang Peng-juan Ni yuan-yuan Jiang Bo Wang Yi-zhong Lu School of Materials Science and Engineering University of JinanJinan 250022China

A label-free"light-on"colorimetric assay for ascorbic acid(AA)determination is unprecedentedly devised by regulating the intrinsic peroxidase-mimetic activity of the Fe-MIL-88.AA can reduce Fe(Ⅲ)presented in Fe-MIL-88 to Fe(Ⅱ),which will dramatically promote the hydroxyl radicals(·OH)conversion efficiency of H_(2)O_(2)through a Fenton reaction,and effectually accelerate the Fe-MIL-88 catalyzed chromogenic reaction of 3,3′,5,5′-tetramethylbenzidine(TMB)-H_(2)O_(2).Redox reaction between AA and H_(2)O_(2)also can produce·OH and promote this chromogenic reaction.Thus,quantification of AA is real-ized via determining the enhanced colorimetric signals.The linear range is 0.5−40μM with a detection limit of 0.12μM.Furthermore,the straightforward yet effective colorimetric strategy with the merits of simplicity,cost-effectiveness,high accuracy,specificity,and sensitivity is widely utilized to determine the concentrations of AA in serological tests.

关键词： Ascorbic acid Colorimetric Fe-MIL-88 Hydroxyl radicals Fenton reaction

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Screening and bioinformatics analysis of diabetic peripheral neuropathy-related genes in women

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Journal of Hainan Medical University 2020年第1期26卷 52-56页

作者： Yi Zhang Zhen Qiu Zhong-Yuan Xia \Department of Anesthesiology People's Hospital Wuhan University Wuhan Hubei 430060

Objective: To obtain the key genes and signal pathways of diabetic peripheral neuropathy (DPN) through bioinformatics analysis of related gene chips in the GEO database. Methods: The DPN-related gene chip was downloaded from the GEO database, and the differential genes (DEGs) between DPN female patients and the normal control group were analyzed and visualized using R language. According to the gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG), DEGs were annotated, their functions and related pathways were predicted, and a protein interaction network was constructed using the STRING database to screen for core genes. Results: The analysis chip GSE95849 obtained 4746 DEGs of which 2218 genes were up-regulated and 2528 genes were down-regulated. Among them, TFAP2C, ESR1, CX3CR1, and FGL2 are at the core site of protein interaction. Conclusions: Differential genes are mainly involved in the MAPK pathway. They participate in the pathogenesis of DPN through blood glucose homeostasis, inflammatory effects, and neuronal development, providing new ideas for the diagnosis and treatment of DPN.

关键词： diabetic peripheral neuropathy bioinformatics ESR1 gene CX3CR1 gene FGL2 gene

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The role of glycogen synthase kinase 3 beta in brain injury induced by myocardial ischemia/reperfusion injury in a rat model of diabetes mellitus

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Neural Regeneration Research 2017年第10期12卷 1632-1639页

作者： Bo Zhao Wen-wei Gao Ya-jing Liu Meng Jiang Lian Liu Quan yuan Jia-bao Hou Zhong-Yuan Xia Department of Anesthesiology Renmin Hospital of Wuhan UniversityWuhanHubei ProvinceChina Department of Critical Care Medicine Renmin Hospital of Wuhan UniversityWuhanHubei ProvinceChina

Myocardial ischemia/reperfusion injury can lead to severe brain injury.Glycogen synthase kinase 3 beta is known to be involved in myocardial ischemia/reperfusion injury and diabetes mellitus.However,the precise role of glycogen synthase kinase 3 beta in myocardial ischemia/reperfusion injury-induced brain injury is unclear.In this study,we observed the effects of glycogen synthase kinase 3 beta on brain injury induced by myocardial ischemia/reperfusion injury in diabetic rats.Rat models of diabetes mellitus were generated via intraperitoneal injection of streptozotocin.Models of myocardial ischemia/reperfusion injury were generated by occluding the anterior descending branch of the left coronary artery.Post-conditioning comprised three cycles of ischemia/reperfusion.Immunohistochemical staining and western blot assays demonstrated that after 48 hours of reperfusion,the structure of the brain was seriously damaged in the experimental rats compared with normal controls.Expression of Bax,interleukin-6,interleukin-8,terminal deoxynucleotidyl transferase d UTP nick end labeling,and cleaved caspase-3 in the brain was significantly increased,while expression of Bcl-2,interleukin-10,and phospho-glycogen synthase kinase 3 beta was decreased.Diabetes mellitus can aggravate inflammatory reactions and apoptosis.Ischemic post-conditioning with glycogen synthase kinase 3 beta inhibitor lithium chloride can effectively reverse these changes.Our results showed that myocardial ischemic post-conditioning attenuated myocardial ischemia/reperfusion injury-induced brain injury by activating glycogen synthase kinase 3 beta.According to these results,glycogen synthase kinase 3 beta appears to be an important factor in brain injury induced by myocardial ischemia/reperfusion injury.

关键词： nerve regeneration myocardial ischemia/reperfusion injury brain injury glycogen synthase kinase 3 beta ischemic post-conditioning diabetes mellitus neural regeneration

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