检索结果-南通市图书馆

Efficient and genotype independent maize transformation using pollen transfected by DNA-coated magnetic nanoparticles

引用

Journal of Integrative Plant Biology 2022年第6期64卷 1145-1156页

作者： Zuo-Ping Wang zhong-Bao Zhang Deng-Yu Zheng tong-tong Zhang Xiang-Long Li Chun Zhang Rong Yu Jian-Hua Wei zhong-yi Wu Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology Institute of BiotechnologyBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China College of Life Sciences Capital Normal UniversityBeijing 100048China

Current gene delivery methods for maize are limited to specific genotypes and depend on timeconsuming and labor-intensive tissue culture ***,we report a new method to transfect maize that is culture-free and genotype *** enhance efficiency of DNA entry and maintain high pollen viability of 32%-55%,transfection was performed at cool temperature using pollen pretreated to open the germination aperture(40%–55%).Magnetic nanoparticles(MNPs)coated with DNA encoding either red fluorescent protein(RFP),β-glucuronidase gene(GUS),enhanced green fluorescent protein(EGFP)or bialaphos resistance(bar)was delivered into pollen grains,and female florets of maize inbred lines were *** fluorescence was detected in 22%transfected pollen grains,and GUS stained 55%embryos at 18 d after *** fluorescence was detected in both silk filaments and immature *** T1 generation of six inbred lines showed considerable EGFP or GUS transcripts(29%–74%)quantitated by polymerase chain reaction,and 5%–16%of the T1 seedlings showed immunologically active EGFP or GUS ***,1.41%of the bar transfected T1 plants were glufosinate resistant,and heritable bar gene was integrated into the maize genome effectively as verified by DNA *** results demonstrate that exogenous DNA could be delivered efficiently into elite maize inbred lines recalcitrant to tissue culture-mediated transformation and expressed normally through our genotype-independent pollen transfection system.

关键词： exogenous genes genotype independence magnetic nanoparticles maize inbred lines pollen transfection pollen aperture

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Studies of internal magnetic fluctuation in the HL-1M tokamak

引用

核聚变与等离子体物理 2005年第1期25卷 42-48页

作者： ZHENGYong-zhen DINGXuan-tong LIWen-zhong SouthwesternInstituteofPhysics Chengdu610041

The transport of runaway electrons in a hot plasma can be comparatively easily measured in steady state or perturbation experiments, which provide runaway electron diffusivity Dr. The diffusivity can be interpreted in terms of the magnetic fluctuation level. The runaway electron diffusion coefficient is determined by internal magnetic fluctuations. The internal magnetic fluctuations level （r/BT） is estimated to be about （2～4） ×10-4 in the HL-1M plasma. The results demonstrate the effectiveness of using runaway transport techniques for determining internal magnetic fluctuations. A profile of the magnetic fluctuation level in the HL-1M tokamak can be estimated from Dr.

关键词：内禀磁波动扩散系数热等离子体稳定性失控电子传输

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Market Equilibrium Based on Cloud-edge Collaboration

引用

CSEE Journal of Power and Energy Systems 2024年第1期10卷 96-104页

作者： tong Cheng Haiwang zhong Qing Xia the State Key Laboratory of Power Systems the Department of Electrical EngineeringTsinghua UniversityBeijing 100084China

Market participants can only bid with lagged information disclosure under the existing market mechanism,which can lead to information asymmetry and irrational market behavior,thus influencing market *** promote rational bidding behavior of market participants and improve market efficiency,a novel electricity market mechanism based on cloudedge collaboration is proposed in this *** market information,called residual demand curve,is published to market participants in real-time on the cloud side,while participants on the edge side are allowed to adjust their bids according to the information disclosure prior to closure *** proposed mechanism can encourage rational bids in an incentive-compatible way through the process of dynamic equilibrium while protecting participants’*** paper further formulates the mathematical model of market equilibrium to simulate the process of each market participant’s strategic bidding behavior towards equilibrium.A case study based on the IEEE 30-bus system shows the proposed market mechanism can effectively guide bidding behavior of market participants,while condensing exchanged information and protecting privacy of participants.

关键词： Cloud-edge collaboration market mechanism residual demand curve strategic bidding

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Sequential maneuvering decisions based on multi-stage influence diagram in air combat

引用

Journal of Systems Engineering and Electronics 2007年第3期18卷 551-555页

作者： zhong Lin tong Ming'an zhong Wei Zhang Shengyun Dept. of Electronic Information Northwestern Polytechnical Univ. Xi'an 710072 P. R. China School of Electronic Science Jiangxi Univ. of Finance and Economics Nanchang 330013 P. R. China

A multi-stage influence diagram is used to model the pilot＇s sequential decision making in one on one air combat. The model based on the multi-stage influence diagram graphically describes the elements of decision process, and contains a point-mass model for the dynamics of an aircraft and takes into account the decision maker＇s preferences under uncertain conditions. Considering an active opponent, the opponent＇s maneuvers can be modeled stochastically. The solution of multistage influence diagram can be obtained by converting the multistage influence diagram into a two-level optimization problem. The simulation results show the model is effective.

关键词： multi-stage influence diagram air combat maneuvering decision hierarchical optimization.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

High-temperature oxidation behavior and degradation mechanism of chromium-coated zirconium alloy cladding: a review

引用

Tungsten 2025年第1期 50-70页

作者： yi Li Bei-Qi Wang Xin-yin Zhang Shi-Jie Wang Cong-yi Li Hui-Long Yang Ran-Ran Su tong Liu School of Mechanical Engineering Shanghai Jiao Tong University College and Graduate School of Arts and Sciences University of Virginia Global Institute of Future Technology Shanghai Jiao Tong University

Accident-tolerant fuels(ATF) have been extensively studied to reduce the production rate and total amount of heat and hydrogen generated from high-temperature steam oxidation during severe ***-coated zirconium alloy cladding has become one of the most promising candidates for ATF because of its excellent corrosion and oxidation *** paper provides a comprehensive overview of the research progress on oxidation behaviors and degradation mechanisms in chromium-coated zirconium alloy cladding under high-temperature *** techniques to strengthen the oxidation resistance are highlighted and ***,challenges and opportunities for various future directions are addressed.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

High-accuracy calibration for multi-laser powder bed fusion via in situ detection and parameter identification

引用

Advances in Manufacturing 2022年第4期10卷 556-570页

作者： Qi zhong Xiao-Yong Tian Xiao-Kang Huang Zhi-Qiang tong yi Cao Di-Chen Li State Key Laboratory of Manufacturing Systems Engineering Xi’an Jiaotong UniversityXi’an710049People’s Republic of China

Multi-laser powder bed fusion(ML-PBF)adopts multiple laser-scanner systems to increase the build envelope and build speed,but its calibration is an iterative and time-consuming *** particular,multiple large-scale scan fields have a complex distortion in the overlap area,challenging the calibration *** this study,owing to the enormous workload and alignment problems in the calibration of multiple scan fields,a novel calibration system is designed in this study to realize in situ auto-detection of numerous laser spots in the build chamber to ensure high efficiency and ***,because the detectable area could not cover the entire build area and the detection data still contained errors,a virtual laser-scanner system was established by identifying the assembly defects and galvo nonlinearities of the ML-PBF system from the detection data,which served as the system's controller to improve calibration *** multi-field alignment error was less than 0.012%,which could avoid the intersection and separation of scan paths in multi-laser scanning and therefore meet the requirements for high-precision ***,the reliability of the method was verified theoretically using principal component analysis.

关键词： Powder bed fusion Multi-laser technology Galvo calibration Assembly defects System identification

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Application of Eigenkets of Bosonic Creation Operator in Deriving Some New Formulas of Associated Laguerre Polynomials

引用

Communications in Theoretical Physics 2008年第8期50卷 315-320页

作者： FAN Hong-yi WANG tong-tong Department of Material Science and Engineering University of Science and Technology of China Hefei 230026 China Department of Physics Shanghai Jiao Tong University Shanghai 200030 China

Using the resolution of unity composed of bosonic creation operator＇s eigenkets and annihilation operator＇s un-normalized eigenket, which is a new quantum mechanical representation in contour integration form, we derive new contour integration expression of associated Laguerre polynomials L^ρm （｜z｜^2） and its generalized generating function formula. A series of recursive relations regarding to L^ρm （｜z｜^2） are also deduced in the context of the Fock representation by algebraic method.

关键词： bosonic creation operator Laguerre polynomials contour integration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Current Reversal in a Flashing Correlation Ratchet

引用

Chinese Physics Letters 1999年第7期16卷 475-477页

作者： ZHAO tong-jun ZHAN Yong ZHUO yi-zhong WU Xi-zhen Department of Applied Mathematics and Physics Hebei University of Technology Tianjin 300130 China Institute of Atomic Energy Beijing 102413 Institute of Theoretical Physics Chinese Academy of SciencesBeijing 100080

A flashing correlation ratchet model is proposed,in which the transport of Brownian particles driven by additive colored noise of Ornstein-Uhlenbeck type in a flashing ratchet is studied by Monte Carlo *** stationary current vs the noise strength and the correlation time is obtained and *** a fixed rate of potential flashing,the novel phenomena of current reversal and reentry *** conditions for the occurrence of these phenomena are *** in fluence of the flipping rate is also *** finding might be relevant to the fact that the protein motors with similar structure can move in opposite directions on the same kind of microtubule.

关键词： directions noise Flash

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neurocognitive Correlates of Statistical Learning of Orthographic-Semantic Connections in Chinese Adult Learners

引用

Neuroscience Bulletin 2020年第8期36卷 895-906页

作者： Xiuhong tong yi Wang Shelley Xiuli tong Department of Psychology The Education University of Hong KongHong KongChina Institute of Psychological Sciences Hangzhou Normal UniversityHangzhou 310000China Human Communication Developmentand Information SciencesFaculty of EducationThe University of Hong KongHong KongChina

We examined the neural correlates of the statistical learning of orthographic-semantic connections in Chinese adult *** event-related potentials(ERPs) were recorded while participants were exposed to a sequence of artificial logographic characters containing semantic radicals carrying low,moderate,or high levels of semantic *** behavioral results showed that the mean accuracy of participants’ recognition of previously exposed characters was 63.1% that was significantly above chance level(50%),indicating the statistical learning of the regularities of semantic *** ERP data revealed a temporal sequence of the neural process of statistical learning of orthographic-semantic connections,and different brain indexes were found to be associated with this processing,i.e.,a clear N170-P200-N400 *** N170,the larger negative amplitudes were evoked by the high and moderate consistency than the low *** P200,the mean amplitudes elicited by the moderate and low consistency were larger than the high *** contrast,a larger N400 amplitude was observed in the low than moderate and high consistency;and more negative amplitude was elicited by the moderate than high *** propose that the initial potential shifts(N170 and P200) may reflect orthographic or graphic form identification,while the later component(N400) may be associated with semantic information analysis.

关键词： Orthography-semantic connection Statistical learning N170 P200 N400

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

企业数字化转型与全球价值链参与

引用

管理工程学报 2025年第1期39卷 15-31页

作者：李鑫佟岩曹丰北京理工大学管理学院湖南大学工商管理学院

本文立足“逆全球化”时代数字经济发展迅速的现实背景,利用2010年至2016年中国A股上市公司与中国海关数据库的合并数据,实证检验了企业数字化转型对其全球价值链参与的影响。研究表明,数字化转型能够促进企业更好地融入全球价值链,并... 详细信息

本文立足“逆全球化”时代数字经济发展迅速的现实背景,利用2010年至2016年中国A股上市公司与中国海关数据库的合并数据,实证检验了企业数字化转型对其全球价值链参与的影响。研究表明,数字化转型能够促进企业更好地融入全球价值链,并且该结论在经过一系列稳健性检验后依然成立。影响机制检验发现,企业数字化转型对全球价值链嵌入的提升作用主要通过缓解融资约束和提高生产效率来实现。异质性检验结果表明,当数字化转型基础较好、劳动力需求较高以及成本加成较低时,企业数字化转型对全球价值链参与的影响更为凸显。本文丰富和拓展了企业数字化转型影响机制与经济后果的研究框架,为企业更好地融入全球价值链分工体系、实现高质量发展找到了新的经验证据,对于企业数字化相关政策的制定与调整也具有一定的参考价值。

关键词：数字化转型全球价值链嵌入融资约束生产效率

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：