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抗HPA-3a抗体所致新生儿同种免疫性血小板减少性紫癜一例并文献复习

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中华血液学杂志 2013年第1期34卷 45-48页

作者：周燕钟周琳李丽兰申卫东刘金莲吴国光南宁中心血站南宁输血医学研究所 530003

目的探讨抗血小板特异性抗原（HPA）-3a抗体所致新生儿同种免疫性血小板减少性紫癜（NAITP）的诊断和治疗。方法采用多重PCR及基因测序技术检测1例出血伴血小板减少新生儿及其父母HPA-1～21bw系统基因型，采用流式细胞术（FCM）和血小... 详细信息

目的探讨抗血小板特异性抗原（HPA）-3a抗体所致新生儿同种免疫性血小板减少性紫癜（NAITP）的诊断和治疗。方法采用多重PCR及基因测序技术检测1例出血伴血小板减少新生儿及其父母HPA-1～21bw系统基因型，采用流式细胞术（FCM）和血小板抗原单克隆抗体特异性免疫固定检测技术（MAIPA）检测患儿及其母亲血清血小板特异性抗体并进行特异性鉴定。结果患儿出生后2h出现全身多发皮下出血点、血尿及咖啡色呕吐物。基因分型显示患儿为HPA-3ab、母亲为HPA-3bb、父亲为HPA-3aa；患儿及母亲血清中均含与患儿父亲血小板反应的特异性抗体，经MAIPA技术鉴定为抗HPA-3a抗体。结论发现1例抗HPA-3a抗体所致NAITP患者，通过临床特征分析为该病的诊断和治疗提供借鉴与参考。

关键词：血小板减少,新生儿同种免疫性流式细胞术抗原,人血小板聚合酶链反应

Thermally triggered topological polymerization in diacetylene-functionalized covalent organic framework toward enhanced memristive properties

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Science China Chemistry 2025年

作者： Panke zhou Yuxing Huang Ziyue Yu Cong Zhang Qian Chen Yiping Li Chao lin Xiong Chen State Key Laboratory of Photocatalysis on Energy and Environment Key Laboratory of Advanced Carbon-Based Functional MaterialsCollege of Chemistry Fuzhou University

The development of stimuli-responsive switching memory devices and the elucidation of their switching mechanisms in specific environments are crucial for advancing the field of molecular electronics. Herein, we propose two distinct two-dimensional covalent organic frameworks (COFs), namely Py-EDA and Py-BDA, incorporating acetylene (-C≡C-) or diacetylene(-C≡C-C≡C-) moieties, respectively, targeting fabricate memory devices exhibiting stimuli-responsive switching *** findings demonstrate that the incorporation of acetylene units effectively modulates the electronic band structure and enhances the degree ofπ-conjugation, resulting in devices that exhibit typical bipolar nonvolatile memory performance. Notably,upon thermal treatment, the memory behavior of the ITO/Py-BDA/Ag device transitions to a write-once-read-many-times(WORM) mode, displaying decreased SET voltage, increased ON/OFF current ratio, and remarkable retention reliability and cycle stability. This switching behavior is attributed to the occurrence of interlayer solid-state topological polymerization at 350℃, leading to the transformation of diacetylene columnar arrays into enyne chains within Py-BDA, thereby further promoting interlayer charge transfer and separation.

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Microstructure and Wear/corrosion Resistance of Stainless Steel Laser-alloyed with Mn+W_(2)C, Mn+NiWC and Mn+SiC

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 283-294页

作者： zhou Rui DIAO Xiaogang SUN Yixin School of Metallurgical Engineering Jiangsu University of Science and TechnologyZhangjiagang 215600China CITIC Heavy Cast Iron Industry Co. Ltd.Luoyang 417039China

In-situ formed high Mn steel coating reinforced by carbides was formed by laser surface alloying(LSA).Laser alloyed layers on 1Cr18Ni9Ti steel with Mn+W_(2)C(specimen A),Mn+NiWC(specimen B)and Mn+SiC(specimen C)powders were fabricated to improve the wear and corrosion behavior of 1Cr18Ni9Ti steel blades in high speed *** evolution,phases,element distribution,microhardness,wear and corrosion behavior of the laser alloyed layers were *** indicated that high Mn steel matrix composites with undissolved W_(2)C,WC and other in-situ formed carbides were formed by LSA with Mn+W_(2)C and Mn+NiWC while SiC totally dissolved into the high Mn matrix when adding Mn+*** as the binding phase in Ni-WC powder decreased the crack sensitivity of the alloyed layer as compared with the addition of W_(2)C *** improvement in average microhardness was achieved in the matrix in specimen A,B and C,with the value of 615,602 and 277 HV_(0.5),while that of the substrate was 212 HV_(0.5).The increase of microhardness,wear and corrosion resistance is highly corelated to microstructure,formed phases,type and content of carbides,micro-hardness and toughness of the alloyed layers.

关键词： laser surface alloying stainless steel carbide type microstructure wear and corrosion resistance

Hsa-miR-214-3p inhibits breast cancer cell growth and improves the tumor immune microenvironment by downregulating B7H3

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Oncology research 2025年第1期33卷 103-121页

作者： Yan Lu Kang Wang Yuanhong Peng Meng Chen lin zhong Luji Huang F U Cheng Xindan Sheng Xin Yang Manzhao Ouyang George A Calin Zhiwei He China-America Cancer Research Institute Guangdong Medical University Dongguan 523808 China. GCP Center Shunde Hospital Southern Medical University (The First People's Hospital of Shunde Foshan) Shunde 528300 China. Department of Gastrointestinal Surgery Shunde Hospital Southern Medical University (The First People's Hospital of Shunde Foshan) Shunde 528300 China. The Second School of Clinical Medicine Southern Medical University Guangzhou 510080 China. Department of Translational Molecular Pathology The University of Texas MD Anderson Cancer Center Houston TX 77030 USA. Department of Oncology Shunde Hospital Southern Medical University (The First People's Hospital of Shunde Foshan) Shunde 528300 China.

Background:Immune checkpoint inhibitors play an important role in the treatment of solid tumors, but the currently used immune checkpoint inhibitors targeting programmed cell death-1 (PD-1), programmed cell death ligand-1 (PD-L1), and cytotoxic T-lymphocyte antigen-4 (CTLA-4) show limited clinical efficacy in many breast cancers. B7H3 has been widely reported as an immunosuppressive molecule, but its immunological function in breast cancer patients remains unclear.Methods:We analyzed the expression of B7H3 in breast cancer samples using data from the Cancer Genome Atlas Program (TCGA) and the Gene Expression Omnibus (GEO) databases. MicroRNAs were selected using the TarBase, miRTarBase, and miRBase databases. The regulatory role of the microRNA hsa-miR-214-3p on B7H3 was investigated through dual-luciferase reporter assays, which identified the specific action sites of interaction. The expression levels of B7H3 and hsa-miR-214-3p in human breast cancer tissues and adjacent normal tissues were quantified using Western blotting and quantitative PCR (qPCR). experiments were performed to observe the effects of modulating the expression of B7H3 or hsa-miR-214-3p on breast cancer cell proliferation and apoptosis. Additionally, the regulatory impact of hsa-miR-214-3p on B7H3 was examined. Enzyme-linked immunosorbent assays (ELISA) and flow cytometry were employed to assess the effects of co-cultured breast cancer cells and normal human peripheral blood mononuclear cells (PBMCs) on immune cells and associated cytokines.Results:In breast cancer tissues, the expression level of B7H3 is inversely correlated with that of hsa-miR-214-3p, as well as with the regulatory effects on breast cancercell behavior. Hsa-miR-214-3p was found to inhibit breast cancer cell growth by downregulating B7H3. Importantly, our research identified, for the first time, two binding sites for hsa-miR-214-3p on the 3' UTR of B7H3, both of which exert similar effects independently. Co-culture experime

关键词： B7H3 Breast cancer Hsa-miR-214-3p Immunotherapy

酶联免疫吸附试验与流式细胞术检测血小板自身抗体的比较

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广西医学 2014年第8期36卷 1037-1039页

作者：周燕钟周琳刘金莲广西南宁输血医学研究所南宁市530003

目的比较酶联免疫吸附试验(ELISA)与流式细胞术(FCM)检测血小板自身抗体的特点,探讨其对特发性血小板减少性紫癜(ITP)的诊断意义。方法 42例ITP患者,分别采用FCM和ELISA检测血小板表面及血浆中的抗血小板自身抗体。结果 FCM检出血小板... 详细信息

目的比较酶联免疫吸附试验(ELISA)与流式细胞术(FCM)检测血小板自身抗体的特点,探讨其对特发性血小板减少性紫癜(ITP)的诊断意义。方法 42例ITP患者,分别采用FCM和ELISA检测血小板表面及血浆中的抗血小板自身抗体。结果 FCM检出血小板自身抗体阳性36例,阳性率为85.7%;ELISA检出血小板自身抗体阳性30例,阳性率为71.4%,两种方法阳性率差异无统计学意义(P>0.05),但是FCM和ELISA检测血小板表面抗体阳性率均高于血浆中自身抗体阳性率(P均<0.05)。结论 FCM和ELISA检测结果均准确可靠,且各具特征。FCM具有快速、方便的特点更适合初筛使用,与ELISA联合检测可提高ITP诊断水平。

关键词：血小板减少性紫癜血小板自身抗体酶联免疫吸附实验流式细胞术

造血干细胞移植术后抗-CD36抗体介导的血小板输注无效症和相关病例的实验研究

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中国实验血液学杂志 2018年第2期26卷 541-546页

作者：周燕李丽兰钟周琳刘学军刘金莲申卫东吴国光南宁输血医学研究所广西南宁530007

目的:对造血干细胞移植术后由抗-CD36抗体介导血小板输注无效症和相关病例进行实验研究,对患者和造血干细胞供者的CD36表型和CD36基因,以及相关抗体的特征进行分析和鉴定,探讨患者的血小板输注治疗效果。方法:采用流式细胞术检测患者及... 详细信息

目的:对造血干细胞移植术后由抗-CD36抗体介导血小板输注无效症和相关病例进行实验研究,对患者和造血干细胞供者的CD36表型和CD36基因,以及相关抗体的特征进行分析和鉴定,探讨患者的血小板输注治疗效果。方法:采用流式细胞术检测患者及造血干细胞供者CD36在血小板及单核细胞上的表达;采用本实验室建立的快速血小板抗原单克隆抗体特异性免疫固定检测技术(fast monoclonal antibody-specific immobilization of platelet antigen,F-MAIPA)鉴定患者血清中的血小板抗体;采用测序(polymerase chain reaction sequence-based typing,PCRSBT)方法分析患者及供者CD36基因序列及HPA序列;应用STR-PCR(short tandem repeat polymerase chain reaction,STR-PCR)技术监测患者植入的证据。从CD36缺失血小板库中挑选CD36表达缺失的供者血小板给予患者输注,并监测其血小板计数。结果:造血干细胞供者为CD36+,而患者为I型CD36缺失者。患者移植后血清中存在抗-CD36抗体并排除HLA及HPA相关抗体;患者CD36外显子测序提示为1个新的CD36突变基因,即CD36Exon 6-1G>C纯合子(突变位于剪切位点)突变基因所致的CD36缺失。移植后18 d患者的STR位点、HPA以及CD36型别已完全转化为供者型别(完全嵌合),移植后96 d患者血小板、单个核细胞上已有CD36表达。给该患者输注CD36缺失血小板后血小板计数明显提高。结论:对造血干细胞移植术后由抗-CD36抗体引起的输血小板无效症,须对CD36在移植中的同种免疫反应给予足够重视。为了确保血小板输注有效,对CD36缺失患者需给予CD36缺失血小板输注。

关键词：造血干细胞造血干细胞移植抗-CD36抗体血小板输注无效

位于CD36基因剪切位点的2个新突变及其导致CD36缺失的分子基础

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中国实验血液学杂志 2020年第6期28卷 2056-2065页

作者：李丽兰陈洁润蒋丽红钟周琳黎海燕周燕卢芳吴国光南宁输血医学研究所广西南宁530003 南宁中心血站广西南宁530003

目的:对在广西人群中发现的2个位于CD36剪切位点的新基因突变,以及它们导致CD36缺失的分子基础和在人群中的发生率进行研究。方法:对在广西人群中发现的2例CD36缺失个体(HHC和WGM)使用DNA测序和cDNA克隆测序,检测他们的CD36外显子序列及... 详细信息

目的:对在广西人群中发现的2个位于CD36剪切位点的新基因突变,以及它们导致CD36缺失的分子基础和在人群中的发生率进行研究。方法:对在广西人群中发现的2例CD36缺失个体(HHC和WGM)使用DNA测序和cDNA克隆测序,检测他们的CD36外显子序列及mRNA蛋白编码区序列,对所发现的CD36 mRNA异常转录本建立真核表达细胞株并用Western blot实验验证异常转录本对CD36表达的影响。对所发现的新突变基因建立DNA PCR-SSP基因分型方法,在广西地区的110名CD36缺失个体群和广西地区随机的296名人群间展开人群分布调查。结果:在HHC和WGM个体中,发现了CD36剪切位点新突变CD36 c.430-1G>C,且在WGM个体中还发现了CD36 c.1006+2T>G的剪切位点新突变。cDNA克隆测序显示,CD36 c.430-1G>C可导致c.429_430ins[430-17_430-2;C](***144fsTer1)和c.430_609del(***144_Pro203del,GenBank注册号:HM217023.1)2种CD36 mRNA异常转录本的产生,而CD36 c.1006+2T>G可导致c.819_1006del(***274GlufsTer16)(GenBank注册号:HM217025.1)CD36 mRNA异常转录本的产生。通过建立的真核表达细胞株及Western blot实验验证了以上3种异常转录本均可导致CD36表达缺失。对所发现的2个剪切位点突变基因展开的人群发生率的研究显示,在110名CD36缺失个体群和随机的296名人群中,CD36 c.430-1G>C突变发生率分别为10.91%(12/110)和1.35%(4/296),而CD36 c.1006+2T>G突变的发生率分别为2.73%(3/110)和0(0/296)。结论:本研究发现了2个位于CD36基因剪切位点的新突变,初步阐明了它们导致CD36缺失的分子基础及在广西人群中的分布特征,为中国人群CD36缺失的分子机制及特征研究提供了实验和理论依据。

关键词： CD36缺失剪切位点突变分子机制及特征人群分布

药物(奎宁)依赖性血小板抗体检测技术的建立及国际合作评估

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中国临床药理学杂志 2015年第19期31卷 1958-1960页

作者：钟周琳周燕刘金莲李丽兰申卫东吴国光南宁输血医学研究所南宁530007

目的建立药物(奎宁)依赖性血小板抗体检测的技术并组织国际实验室进行评估。方法建立药物依赖性抗体流式细胞术及药物依赖性抗体血小板抗原单克隆抗体特异性免疫固定检测技术,向各参与的实验室提供检测方案,奎宁依赖性血小板抗体血清、... 详细信息

目的建立药物(奎宁)依赖性血小板抗体检测的技术并组织国际实验室进行评估。方法建立药物依赖性抗体流式细胞术及药物依赖性抗体血小板抗原单克隆抗体特异性免疫固定检测技术,向各参与的实验室提供检测方案,奎宁依赖性血小板抗体血清、血小板糖蛋白单克隆抗体、阴性对照及药物奎宁,各实验室按照给定的方案对样本进行检测,对结果做统计比较。结果参加评估实验室对3个评估样本检测的准确率:药物依赖性抗体流式细胞术分别为91.67%,100%,91.67%;药物依赖性抗体血小板抗原单克隆抗体特异性免疫固定检测技术均为100%。所建立的2种检测技术,经国际23家实验室评估,获得良好的灵敏度和特异性。结论该技术可应用于药物诱导免疫性血小板减少症的实验室诊断。

关键词：药物依赖性血小板抗体药物诱导的血小板减少症药物依赖性抗体流式细胞术药物依赖性抗体血小板抗原单克隆抗体特异性免疫固定检测技术

抗-c、抗-E、抗-Jk^(a)和抗-Fy^(a)复杂联合抗体的鉴定

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中国实验血液学杂志 2023年第5期31卷 1475-1480页

作者：马婷婷刘学军黄宝甲周燕莫秋红钟周琳刘金莲南宁输血医学研究所南宁中心血站广西南宁530007

目的:探讨多种血型血清学方法的联合运用在复杂抗体鉴定中的作用。方法:采用盐水法和微柱凝集法检测患者的血型抗原,采用盐水法筛选及鉴定患者血清中的Ig M类抗体,采用凝聚胺法、抗球蛋白法、微柱凝集法、酶法和吸收放散法筛选及鉴定Ig ... 详细信息

目的:探讨多种血型血清学方法的联合运用在复杂抗体鉴定中的作用。方法:采用盐水法和微柱凝集法检测患者的血型抗原,采用盐水法筛选及鉴定患者血清中的Ig M类抗体,采用凝聚胺法、抗球蛋白法、微柱凝集法、酶法和吸收放散法筛选及鉴定Ig G类抗体。结果:检出1例患者血型为B型/CCDee/Jk(a-b+)/Fy(a-b+)。患者血清与谱细胞反应,在盐水介质中呈抗-E格局;在微柱凝集卡中全阳性,但与经木瓜酶处理的谱细胞反应出现2个阴性。参照格局表考虑存在抗-c、E和抗-Jk^(a)抗体,以及1种易被木瓜酶破坏的血型抗原对应的抗体。选用特定表型的红细胞对患者血清进行吸收放散试验,检出Ig G类抗-c、抗-E、抗-Jk^(a)和抗-Fy^(a)抗体。结论:联合运用多种血型血清学方法证实该患者血清中存在Ig M类抗-E和Ig G类抗-c、抗-E、抗-Jk^(a)及抗-Fy^(a)联合抗体。

关键词：血型血清学方法抗体鉴定抗-c 抗-E 抗-Jk^(a) 抗-Fy^(a)