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Journal of Zhejiang University-Science C(Computers and Electronics) 2011年第1期12卷 25-35页

作者： Qing-chao WANG jian-zhong zhang School of Energy Science and Engineering Harbin Institute of Technology Harbin 150001 China SINOPEC Safety Engineering Institute Qingdao 266071 China

This paper deals with Wiener model based predictive control of a pH neutralization *** dynamic linear block of the Wiener model is parameterized using Laguerre filters while the nonlinear block is constructed using least squares support vector machines (LSSVM).Input-output data from the first principle model of the pH neutralization process are used for the Wiener model *** results show that the proposed Wiener model has higher prediction accuracy than Laguerre-support vector regression (SVR) Wiener models,Laguerre-polynomial Wiener models,and linear Laguerre *** identified Wiener model is used here for nonlinear model predictive control (NMPC) of the pH neutralization *** set-point tracking performance of the proposed NMPC is compared with those of the Laguerre-SVR Wiener model based NMPC,Laguerre-polynomial Wiener model based NMPC,and linear model predictive control (LMPC).Validation results show that the proposed NMPC outperforms the other three controllers.

关键词： Wiener model Nonlinear model predictive control (NMPC) pH neutralization process Laguerre filters Least squares support vector machines (LSSVM)

Successful isolation of Helicobacter pylori after prolonged incubation from a patient with failed eradication therapy

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World Journal of Gastroenterology 2009年第12期15卷 1528-1529页

作者： Yan Yin Li-Hua He jian-zhong zhang Department of Diagnosis National Institute for-Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China

Helicobacter pylori(H pylori),a gastric pathogen,is a major cause of chronic gastritis and peptic ulcer disease,and is an important risk factor for the development of gastric *** of the bacterium from gastric biopsy is essential for the determination of drug resistance of H ***,the isolation rates of H pylori from infected individuals vary from 23.5%to 97% due to a number of factors such as biopsy preparation,cultural environment,medium and the method *** the present case,we found that a prolonged incubation period of up to 19 d allowed successful isolation of H pylori from a patient who received triple therapy that failed to eradicate the bacterium.

关键词： Helicobacter pylori Isolation Eradication

Development and application of a real-time polymerase chain reaction method for Campylobacter jejuni detection

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World Journal of Gastroenterology 2013年第20期19卷 3090-3095页

作者： Mao-Jun zhang Bo Qiao Xue-Bin Xu jian-zhong zhang State Key Laboratory for Infectious Disease Prevention and Control and National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and Prevention Shanghai Municipal Center for Disease Control and Prevention

AIM:To develop a real-time polymerase chain reaction(PCR) method to detect and quantify Campylobacter jejuni(***) from stool ***:Primers and a probe for real-time PCR were designed based on the specific DNA sequence of the hipO gene in *** specificity of the primers and probe were tested against a set of Campylobacter *** other enteric *** optimal PCR conditions were determined by testing a series of conditions with standard a *** *** detection limits were obtained using purified DNA from bacterial culture and extracted DNA from the stool *** hundred and forty-two specimens were analyzed for the presence of *** by direct bacterial culture and real-time ***:The optimal PCR system was determined using reference DNA templates,1 × uracil-DNA glycosylase,3.5 mmol/L MgCl 2,1.25 U platinum Taq polymerase,0.4 mmol/L PCR nucleotide mix,0.48 μmol/L of each primer,0.2 μmol/L of probe and 2 μL of DNA template in a final volume of 25 μ*** PCR reaction was carried as follows:95 ℃ for 4 min,followed by 45 cycles of 10 s at 95 ℃ and 30 s at 59 ℃.The detection limit was 4.3 CFU/mL using purified DNA from bacterial culture and 10 3 CFU/g using DNA from stool ***(8.3%,20/242) *** strains were isolated from bacterial culture,while 41(16.9%,41/242) samples were found to be positive by realtime *** sequencing of the PCR product indicated the presence of *** in the *** mixed infection of *** and Salmonella was detected in one specimen and the PCR test for this specimen was ***:The sensitivity of detection of *** from stool specimens was much higher using this PCR assay than using the direct culture method.

关键词： Campylobacter jejuni Real time polymerase chain reaction Application

A Leguminous Shrub (Caragana microphylla) in Semiarid Sandy Soils of North China

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Pedosphere 2006年第3期16卷 319-325页

作者： zhang Tong-Hui SU Yong-zhong CUI jian-Yuan zhang Zhi-Hui CHANG Xue-Xiang Cold and Arid Regions Environmental and Engineering Research Institute Chinese Academy of Sciences Lanzhou 730000 （China）

Caragana microphylla Lam., a pioneer leguminous shrub species for vegetation re-establishment, is widely distributed in the semi-fixed and fixed sandy lands of the Horqin region. Some soil chemical and physical properties were measured under the canopy of C. microphylla and in the adjacent open areas to determine the effects of individual shrubs on soil properties. The influence of isolated C. microphylla on chemical and physical properties of the topsoil was significantly different between plots under the shrub canopy and in the shrub interspaces. Beneath the shrub canopy greater amounts of fine particle fractions, a higher water-holding capacity, and a lower bulk density, as well as higher aboveground and belowground litter biomass were found. Soil organic C and total N concentrations were 23%-31.6% and 14%-27.2% higher under the shrub canopies than in the shrub interspaces, respectively, giving rise to 'islands of fertility'. In a desertified sandy grassland ecosystem, C. microphylla was believed to play a major role in organic C sequestration, N accumulation, and the hydrologic cycle. Additionally, it has been found to be of ecological importance for vegetative restoration and reversal of desertification.

关键词： Caragana microphylla Lam. degraded sandy grassland soil chemical properties soil physical properties

Primary antibiotic resistance of Helicobacter pylori strains isolated from patients with dyspeptic symptoms in Beijing: A prospective serial study

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World Journal of Gastroenterology 2015年第9期21卷 2786-2792页

作者： Yue-Xi zhang Li-Ya Zhou Zhi-Qiang Song jian-zhong zhang Li-Hua He Yu Ding Department of Gastroenterology Beijing Sixth Hospital Department of Gastroenterology Peking University Third Hospital State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and Prevention

AIM:To determine the resistance patterns of Helicobacter pylori(***) strains isolated from patients in Beijing and monitor the change of antibiotic resistance over ***:In this prospective,serial and crosssectional study,*** cultures were successfully obtained from 371 and 950 patients(never receiving eradication) during 2009-2010 and 2013-2014,*** to amoxicillin,clarithromycin,metronidazole,levofloxacin,tetracycline,and rifampicin was determined by Epsilometer ***:The resistance rates of isolates obtained during 2009-2010 were 66.8%,39.9%,34.5%,15.4%,6.7%,and 4.9% to metronidazole,clarithromycin,levofloxacin,rifampicin,amoxicillin and tetracycline,respectively; and the corresponding rates for isolates during 2013-2014 were 63.4%,52.6%,54.8%,18.2%,4.4% and 7.3%,*** resistance rates to clarithromycin and levofloxacin were significantly increased after four *** 2009-2010,14.6% of *** isolates were susceptible to all tested antibiotics,with mono(33.7%),double(28.3%),triple(16.7%),quadruple(6.2%),quintuple(0.3%) and sextuple resistance(0.3%) also being *** 2013-2014,9.4% were susceptible to all tested antibiotics,and mono(27.6%),double(28.4%),triple(24.9%),quadruple(7.3%),quintuple(2.3%) and sextuple resistance(0.1%) was also *** multiple resistant *** isolates were found during ***(to levofloxacin and metronidazole),age(to levofloxacin) and endoscopic findings(to clarithromycin) were independent factors influencing antibiotic ***:*** resistance to commonly used antibiotics in Beijing is high with increased multiple antibiotic resistance.

关键词： Helicobacter pylori Antibiotic resistance Beijing

OVER-EXPRESSION OF EXTRACELLULAR SIGNAL-REGULATED KINASE IN VASCULAR SMOOTH MUSCLE CELL OF HYPERTENSIVE RATS

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Chinese Medical Sciences Journal 2006年第1期21卷 36-40页

作者： Li Jing jian-zhong zhang Yi-li Wang Feng-ying Guo Institute of Immunopathology School of Life Science ＆ Technology Xi＇an Jiaotong University Xi＇an 710061 Department of Pathology Ningxia Medical College Yinchuan 750004

Objective To investigate whether extracellular signal-regulated kinase (ERK1/2) was involved in changes of vascular smooth muscle cell (VSMC) under *** Two-kidney one clip Wistar hypertensive rats (WHR) were sacrificed and their right kidneys were harvested 4 weeks after *** spontaneously hypertensive rats (SHR) were divided into 4, 8, and 16 weeks old groups (SHR4w, SHR8w, and SHR16w), *** control group were sham operated age-matched Wistar *** technique and Western blotting were applied to study ERK1/2 protein expression in VSMC of the renal vascular trees in WHR, SHR, and control *** Blood pressure in two-kidney one clip WHR obviously increased at one week after surgery, and reached to 198.00±33.00 mm Hg at the end of experiment, significantly higher than that in the control rats (P<0.01).Blood pressure in SHR4w (108.00±11.25 mm Hg) was similar to that in the ***, it rose to 122.25±21.75 mm Hg in SHR8w, and even up to 201.75±18.00 mm Hg in SHR16w, which were significantly higher than that of both the SHR4w and the controls (P<0.01).The rate and degree of glomerular fibrosis in WHR were significantly higher than controls (P<0.05).Hyaline degeneration of the afferent arterioles was found in *** contrast, either fibrosis of glomerulus or hyaline degeneration of the arterioles or protein casts was not observed in SHR4w, SHR8w, and *** staining results showed expression of ERK1 was similar to that of *** positive rates of ERK2 staining in VSMC of afferent arterioles, interlobular, interlobar, and arcuate arteries in two-kidney one clip WHR were significantly higher (7.09%±1.75%, 14.57%±4.58%, 29.44%±7.35%, and 13.63%±3.85%, respectively) than that of the controls(P<0.01).The positive rates of ERK2 staining in VSMC at afferent arterioles, interlobular, interlobar, and arcuate arteries in SHR16w were significantly higher (12.09%±1.40%, 24.17%±6.92%, 32.44%±4.0

关键词： hypertension vascular smooth muscle cell extracellular signal-regulated kinase

A Novel Mutation in the Pyrin Domain of the NOD-like Receptor Family Pyrin Domain Containing Protein 3 in Muckle-Wells Syndrome

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Chinese Medical Journal 2017年第5期130卷 586-593页

作者： jian Hu Yun Zhu jian-zhong zhang Rong-Guang zhang Hou-Min Li Department of Dermatology Peking University People's Hospital Beijing 100044 China National Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China

Background： Cryopyrin-associated periodic syndrome （CAPS） is a group of rare, heterogeneous autoinflammatory disease characterized by interleukin （IL）-1β-mediated systemic inflammation and clinical symptoms involving skin, joints, central nervous system, and eyes. It encompasses a spectrum of three clinically overlapping autoinflammatory syndromes including familial cold autoinflammatory syndrome, Muckle-Wells syndrome （MWS）, and neonatal-onset multisystem inflammatory disease. CAPS is associated with gain-of-function missense mutations in NOD-like receptor family pyrin domain-containing protein 3 （NLRP3）, the gene encoding NLRP3. Moreover, most mutations leading to MWS occurred in exon 3 ofNLRP3 gene. Here, we reported a novel mutation occurred in exon 1 ofNLRP3 gene in an MWS patient and attempted to explore the pathogenic mechanism. Methods： Genetic sequence analysis of NLRP3 was performed in an MWS patient who presented with periodic lever, arthralgia, and multiform skin lesions. NLRP3 was also analyzed in this patient＇s parents and 50 healthy individuals. Clinical examinations including X-ray examination, skin biopsy, bone marrow aspiration smear, and blood test of C-reactive protein （CRP）, erythrocyte sedimentation rate （ESR）, serum levels oflL-1β, immunoglobulin E （lgE）, antineutrophil cytoplasmic antibodies, antinuclear antibodies, and extractable nuclear antigen were also analyzed. The protein structure of mutant NLRP3 inflammasome was calculated by SWISS-MODEL software. Proteins of wild type and mutant components ofNLRP3 inflammasome were expressed and purified, and the interaction abilities between these proteins were tested by surface plasmon resonance （SPR） assay. Results： X-ray examination showed no abnormality in the patient＇s knees. Laboratory tests indicated an elevation of CRP （233.24 nag/L） and ESR （67 mm/h） when the patient had fever. Serum IL-1β increased to 24.37 pg/ml, and serum lgE was higher than 2500.00 IU/ml. Other blood tests were normal.

关键词： Muckle-Wells Syndrome Mutation NOD-like Receptor Family Pyrin Domain-containing Protein 3 Pyrin Domain

Effects of Appropriate Prolonged Sacral Neuromodulation Testing in Improving Implantation Rate of a Permanent Implantable Pulse Generator in Patients with Refractory Lower Urinary Tract Dysfunctions in China's Mainland

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Chinese Medical Journal 2017年第4期130卷 439-444页

作者： Peng zhang jian-zhong zhang Li-Yang Wu Xiao-Dong zhang Department of Urology Beijing Chaoyang Hospital Capital Medical University Beijing 100020 China

Background： Sacral neuromodulation （SNM） has become an effective method for treating lower urinary tract voiding dysfunction during the past 20 years. Because of the expensive cost, the number of implantable pulse generator （IPG） implantations per year in China is far lower than that in Western developed countries since 2012. This study was to summarize the effects of the appropriate prolonged SNM testing time in improving the implantation rate of a permanent IPG in patients with refractory lower urinary tract symptoms （LUTS） in China's Mainland. Methods： From January 2013 to June 2016, 51 patients with refractory LUTS received SNM therapy, in this study, we compared the conversion rate 2 weeks after the Stage I test and final actual conversion rate. We also observed the complications （such as pain, infection, and electrode displacement） and effectiveness. We tried to improve an appropriate prolonged test time which was favorable for improving the SNM conversion rate while ensuring safety and effectiveness. Results： Among 51 patients receiving SNM therapy, 19 patients （mean age 45.0 ±16.9 years） had poor Stage I test results, and on an average, the electrode was removed 27.4 ± 9.6 days after the surgery. In one patient, the electrode was removed within 2 weeks; when the remaining 18 patients were questioned 2 weeks after testing, none of the patients wanted to terminate the test, and all the 18 patients desired to prolong the testing time to further observe the treatment effect. The remaining 32 patients （mean age 46.7 ± 15.3 years） received Stage If permanent implantation at 19.6 ± 10.4 days after the surgery. The overall Stage Ⅰ-Ⅱ conversion was 62.7% （32/51） in this study. Within 2 weeks after the surgery, only eight patients received Stage II permanent implantation, and the conversion rate was only 15.7% （8/51 ）, which was much lower than the overall conversion rate of 62.7%. Nearly 84.4% （27/32） of the patients received Stage 11 implantation within 4 weeks. Non

关键词： Lower Urinary Tract Symptoms Sacral Neuromodulation Wound Infection

Microparticle collection for water purification based on laser-induced convection

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Chinese Physics B 2018年第5期27卷 252-256页

作者： Zhi-Hai Liu Jiao-Jie Lei Yu zhang Ya-Xun zhang Xing-Hua Yang jian-zhong zhang Yun Yang Li-Bo Yuan Photonics Research Center School of Science Harbin Engineering University Harbin 150001 China

Water purification is required for environmental protection. In this paper, we propose and demonstrate a rapid, effective and low-cost approach to collect numerous impurities（microparticles） in water on the basis of laser-induced thermal convection. We introduce a heat source by using a fiber tip, which is fabricated into a non-adiabatic-tapered shape. In order to improve the laser power absorption efficiency, we coat a gold film with a thickness of 300 nm on the fiber tip. Due to absorption, the laser power transferred from the fiber to the water results in thermal convection. The forces generated from the thermal convection drive the microparticles to move towards the fiber tip, thereby performing microparticle collection and achieving water purification. Laser-induced thermal convection provides a simple, high-efficiency and low-cost method of collecting microparticles, which is a suitable and convenient for local water purification.

关键词： optical trapping microstructures and particles water purification

Photonic lantern with multimode fibers embedded

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Research in Astronomy and Astrophysics 2014年第8期14卷 1046-1054页

作者： Hai-Jiao Yu Qi Yan Zong-Jun Huang He Tian Yu jiang Yong-Jun Liu jian-zhong zhang Wei-Min Sun Key Laboratory of In-Fiber Integrated Optics Ministry of EducationHarbin Engineering University

A photonic lantem is studied which is formed by seven multimode fibers inserted into a pure silica capillary tube. The core of the tapered end has a uniform refractive index because the polymer claddings are removed before the fibers are inserted. Consequently, the light distribution is also *** theories describing a slowly varying waveguide and multimode coupling are used to analyze the photonic lantern. The transmission loss decreases as the length of the tapered part increases. For a device with a taper length of 3.4 cm, the loss is about 1.06 dB on average for light propagating through the taper from an inserted fiber to the tapered end and 0.99 dB in the reverse direction. For a device with a taper length of 0.7 cm, the two loss values are 2.63 dB and 2.53 dB, respectively. The results show that it is possible to achieve a uniform light distribution with the tapered end and a low-loss transmission in the device if parameters related to the lantern are reasonably defined.

关键词： techniques radial velocities -- methods laboratory -- instrumentation spectrographs