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Heme oxygenase-1 prevents liver fibrosis in rats by regulating the expression of PPAR_γ and NF-_κB

World Journal of Gastroenterology 2012年第14期18卷 1680-1688页

作者： Hui Yang Long-Feng zhao zhong-fu zhao Yan Wang Jing-Jing zhao Li Zhang Department of Infectious Diseases the First AffiliatedHospital of Shanxi Medical University Taiyuan 030001 ShanxiProvince China Institute of Hepatopathy Changzhi MedicalCollege Changzhi 046000 Shanxi Province China

AIM:To investigate the effects of heme oxygenase(HO)-1 on liver fibrosis and the expression of peroxisome proliferator-activated receptor gamma(PPARγ) and nuclear factor-kappa B(NF-κB) in ***:Sixty Wistar rats were used to construct liver fibrosis models and were randomly divided into 5 groups:group A(normal,untreated),group B(model for 4 wk,untreated),group C(model for 6 wk,untreated),group D [model for 6 wk,treated with zinc protoporphyrin Ⅸ(ZnPP-Ⅸ) from week 4 to week 6],group E(model for 6 wk,treated with hemin from week 4 to week 6).Next,liver injury was assessed by measuring serum alanine aminotransferase(ALT),aspartate aminotransferase(AST) and albumin *** degree of hepatic fibrosis was evaluated by measuring serum hyaluronate acid(HA),type Ⅳ collagen(Ⅳ-C) and by histological ***(Hyp) content in the liver homogenate was *** expres-sion levels of alpha-smooth muscle actin(α-SMA) in liver tissue were measured by real-time quantitative polymerase chain reaction(RT-PCR).The expression levels of PPARγ and NF-κB were determined by RT-PCR and Western ***:The expression of HO-1 increased with the development of *** of HO-1 by hemin significantly attenuated the severity of liver injury and the levels of liver fibrosis as compared with inhibition of HO-1 by ZnPP-Ⅸ.The concentrations of serum ALT,AST,HA and Ⅳ-C in group E decreased compared with group C and group D(P < 0.01).Amount of Hyp and α-SMA in the liver tissues in group E decreased compared with group C(0.62 ± 0.14 vs 0.84 ± 0.07,1.42 ± 0.17 vs 1.84 ± 0.17,respectively,P < 0.01) and group D(0.62 ± 0.14 vs 1.11 ± 0.16,1.42 ± 0.17 vs 2.56 ± 0.37,respectively,P < 0.01).The expression of PPARγ at levels of transcription and translation decreased with the development of fibrosis especially in group D;and it increased in group E compared with groups C and D(0.88 ± 0.15 vs 0.56 ± 0.19,0.88 ± 0.15 vs 0.41 ± 0.11,respectively,P < 0.01).The expr

关键词： Heme oxygenase-1 Peroxisome proliferator-activated receptor gamma Nuclear factor-kappa B Liver fibrosis Hemin

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Inhibition of hepatitis B virus expression and replication by RNA interference in HepG2.2.15

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World Journal of Gastroenterology 2006年第37期12卷 6046-6049页

作者： zhong-fu zhao Hui Yang De-Wu Han Long-Feng zhao Guo-Ying Zhang Yun Zhang Ming-She Liu Institute of Hepatology Changzhi Medical College Changzhi 046000 Shanxi Province China Institute of Hepatology Shanxi Medical University Taiyuan 032000 Shanxi Province China

AIM： To observe the inhibition of hepatitis B virus replication and expression by transfecting vector-based small interference RNA （siRNA） pGenesiI-HBV X targeting HBV X gene region into HepG2.2.15 cells. METHODS：pGenesil-HBV X was constructed and transfected into HepG2.2.15 cells via lipofection. HBV antigen secretion was determined 24, 48, and 72 h after transfection by time-resolved immunofluorometric assays （TRFIA）. HBV replication was examined by fluorescence quantitative PCR, and the expression of cytoplasmic viral proteins was determined by immunohistochemistry. RESULTS： The secretion of HBsAg and HBeAg into the supernatant was found to be inhibited by 28.5% and 32.2% （P 〈 0.01）, and by 38.67% （P 〈 0.05） and 42.86% （P 〈 0.01） at 48 h and 72 h after pGenesil-HBV X transfection, respectively. Immunohistochemical staining for cytoplasmic HBsAg showed a similar decline in HepG2.2.15 cells 48 h after transfection. The number of HBV genomes within culture supernatants was also significantly decreased 48 h and 72 h post-transfection as quantified by fluorescence PCR （P 〈 0.05）. CONCLUSION： In HepG2.2.15 cells, HBV replication and expression is inhibited by vector-based siRNA pGenesil- HBV X targeting the HBV X coding region.

关键词： Hepatitis B virus RNA interference Plasmid vector HepG2.2.15

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Synthesis and Properties of SEPS-g-PEO Copolymers with Varying Branch Lengths

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Chinese Journal of Polymer Science 2018年第8期36卷 934-942页

作者： zhong-fu zhao Pei-Ying Liu Chun-Qing Zhang Wei Liu Yan-Hui Wang Tao Tang Yi-fu Ding Yan-Dong Zhang Fan-Zhi Meng State Key Laboratory of Fine Chemicals School of Chemical EngineeringDalian University of Technology State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied ChemistryChinese Academy of Sciences Department of Mechanical Engineering University of Colorado Boulder

Poly（ethylene oxide）（PEO） was controllably grafted from styrene-b-（ethylene-co-propylene）-b-styrene （SEPS） backbones by combining lithiation of styrenic units and living monomer-activated anionic ring-opening polymerization of ethylene oxide （EO） monomers with the aid of co-initiators triisobutyl aluminum. The as-synthesized SEPS-g-PEO copolymers were characterized by SEC, 1H-NMR, FTIR, SAXS, AFM and DSC. When the branch length is relatively small, increase of PEO fraction leads to the increase of the correlation length between neighboring hard domains, but the degree of correlation reduces. When the branch length is relatively large, the phase-separated structures become random both in terms of size and spatial correlation, and macro-phase separated structures appear. The crystallization behavior of the PEO branches can be effectively inhibited in SEPS-g-PEO, so no significant crystallization takes place until the fraction of PEO branches is 20.1 wt%, which greatly promotes the rapid delivery of hydrophilic drugs in the hot-melting pressure- sensitive adhesives （HMPSAs） based on SEPS-g-PEO. Their cumulative release amount of a model drug could achieve 80%, more than twice the value in the HMPSAs based on linear PEO-containing styrenic block copolymers.

关键词： Living anionic polymerization SEPS-g-PEO copolymers Phase structures Drug delivery

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Multiple pathogenic factor-induced complications of cirrhosis in rats: A new model of hepatopulmonary syndrome with intestinal endotoxemia

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World Journal of Gastroenterology 2007年第25期13卷 3500-3507页

作者： Hui-Ying Zhang De-Wu Han zhong-fu zhao Ming-She Liu Yan-Jun Wu Xian-Ming Chen Cheng Ji Department of pathophysiology Changzhi Medical CollegeChangzhi 046000 Shanxi Province China Institute of Hepatology Shanxi Medical UniversityTaiyuan 030001 Shanxi Province China Department of Medical Microbiology and Immunology Creighton University School of Medicine Omaha NE 68178 United States Department of Medicine University of Southern California Los Angeles CA 90033 United States

AIM： To develop and characterize a practical model of Hepatopulmonary syndrome. （HPS） in rats.METHODS： The experimental animals were randomized into five feeding groups：（1） control （fed standard diet）, （2） control plus intraperitoneal injection with lipopolysaccharide （LPS）, （3） cirrhosis （fed a diet of maize flour, lard, cholesterol, and alcohol plus subcutaneously injection with carbon tetrachloride （CCI4） oil solution）, （4） cirrhosis plus LPS, and （5） cirrhosis plus glycine and LPS. The blood, liver and lung tissues of rats were sampled for analysis and characterization. Technetium 99m-labeled macroaggregated albumin （Tc99m-MAA） was used to test the dilatation of pulmonary microvasculature.RESULTS： Typical cirrhosis and subsequent hepato- pulmonary syndrome was observed in the cirrhosis groups after an 8 wk feeding period. In rats with cirrhosis, there were a decreased PaO2 and PaCO2 in arterial blood, markedly decreased arterial 02 content, a significantly increased alveolar to arterial oxygen gradient, an increased number of bacterial translocated within mesenteric lymph node, a significant higher level of LPS and tumor necrosis factor-α （TNF-α） in plasma, and a significant greater ratio of Tc99m-MAA brain-overlung radioactivity. After LPS administration in rats with cirrhosis, various pathological parameters got worse and pulmonary edema formed. The predisposition of glycine antagonized the effects of LPS and significantly alleviated various pathological alterations.

关键词： Alcohol Carbon tetrachloride Endotoxin High fat diet

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Intestinal endotoxemia plays a central role in development of hepatopulmonary syndrome in a cirrhotic rat model induced by multiple pathogenic factors

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World Journal of Gastroenterology 2007年第47期13卷 6385-6395页

作者： Hui-Ying Zhang De-Wu Han Ai-Rong Su Li-Tong Zhang zhong-fu zhao Jing-Quan Ji Bao-Hong Li Cheng Ji Department of Pathophysiology Changzhi Medical College Changzhi 046000 Shanxi Province China Institute of Hepatology Shanxi Medical University Taiyuan 030001 Shanxi Province China Department of Physiology Bengbu Medical College Bengbu 233030 Anhui Province China Department of Gynaecology Shanxi Modem Women Hospital Taiyuan 030012 Shanxi Province China Department of Medicine University of Southern California Los Angeles CA 90033 United States

AIM: To characterize the correlation between severity of hepatopulmonary syndrome (HPS) and degree of hepatic dysfunction,and to explore how intestinal endotoxemia (IETM) affects the development of HPS in cirrhotic rats. METHODS: Male Wister rats were fed with a diet containing maize flour,lard,cholesterol,and alcohol and injected subcutaneously with CCl4 oil solution every two days for 8 wk to induce typical cirrhosis and development of HPS. The animals were also given a nitric oxide (NO) production inhibitor,Nω-nitro-L-arginine methyl ester (L-NAME) intraperitoneally,and an iNOS inhibitor,aminoguanidine hydrochloride (AG) via gavage daily from the end of the 4th wk to the end of the 6th or 8th wk,or a HO-1 inhibitor,zinc protoporphyrin (ZnPP) intraperitoneally 12 h prior to killing. Blood,liver and lung tissues were sampled. RESULTS: Histological deterioration of the lung paralleled to that of the liver in the cirrhotic rats. The number of pulmonary capillaries was progressively increased from 6.1 ± 1.1 (count/filed) at the 4th wk to 14.5 ± 2.4 (count/filed) at the 8th wk in the cirrhotic rats. Increased pulmonary capillaries were associated with increased blood levels of lipopolysaccharide (LPS)(0.31 ± 0.08 EU/mL vs control 0.09 ± 0.03 EU/mL),alanine transferase (ALT,219.1 ± 17.4 U/L vs control 5.9 ± 2.2 U/L) and portal vein pressure. Compared with normal control animals,the number of total cells in bronchoalveolar lavage fluid (BALF) of the cirrhotic rats at the 8th wk was not changed,but the number of macrophages and the ratio of macrophages to total cells were increased by nearly 2-fold,protein expression of inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) started to increase significantly at the 4th wk,and reached its peak at the 8th wk in the lung of cirrhotic rats. The increase of iNOS expression appeared to be quicker than that of eNOS. NO2-/NO3-was also increased,which was correlated to the increase of iNOS (r = 0.7699,P

关键词： Endotoxin Alcohol Nitric oxide synthase Hemeoxygenase-1 Capillary tacrophage Cirrhosis Hepatopulmonary syndrome

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Effect of artesunate supplementation on bacterial translocation and dysbiosis of gut microbiota in rats with liver cirrhosis

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World Journal of Gastroenterology 2016年第10期22卷 2949-2959页

作者： Yun-Xia Chen Li-Na Lai Hui-Ying Zhang Yang-Hui Bi Li Meng Xu-Jiong Li Xiao-Xia Tian Li-Min Wang Yi-Min Fan zhong-fu zhao De-Wu Han Cheng Ji Department of Microbiology Changzhi Medical College Department of Pharmacology Changzhi Medical College Department of Pathophysiology Changzhi Medical College Department of Physiology Changzhi Medical College functional Integrative Laboratory Department of PathophysiologyChangzhi Medical College Institute of Hepatology Changzhi Medical College Institute of Hepatology Shanxi Medical University USC Research Center for Liver Disease Department of MedicineUniversity of Southern California

AIM: To evaluate the effect of artesunate(AS) supplementation on bacterial translocation(BT) and gut microbiota in a rat model of liver cirrhosis. METHODS: Fifty-four male Sprague-Dawley rats were randomly divided into a normal control group(N), a liver cirrhosis group(M) and a liver cirrhosis group intervened with AS(MA). Each group was sampled at 4, 6 and 8 wk. Liver cirrhosis was induced by injection of carbon tetrachloride(CCl4), intragastric administration of 10% ethanol, and feeding a high fat diet. Rats in the MA group were intragastrically administered with AS(25 mg/kg body weight, once daily). Injuries of the liver and intestinal mucosa were assessed by hematoxylineosin or Masson's trichrome staining. Liver index was calculated as a ratio of the organ weight(g) to body weight(g). The gut microbiota was examined by automated ribosomal intergenic-spacer analysis of fecal DNA. BT was assessed by standard microbiological techniques in the blood, mesenteric lymph nodes(MLNs), liver, spleen, and kidney. RESULTS: Compared to group N, the body weight was reduced significantly in groups M and MA due to the development of liver cirrhosis over the period of 8 wk. The body weight was higher in group MA than in group M. The liver indices were significantly elevated at 4, 6 and 8 wk in groups M and MA compared to group N. AS supplementation partially decreased the liver indices in group MA. Marked histopathologic changes in the liver and small intestinal mucosa in group M were observed, which were alleviated in group MA. Levels of pro-inflammatory interleukin-6 and tumor necrosis factor-α were significantly elevated at 8 wk in ileal homogenates in group M compared to group N, which were decreased after AS supplementation in group MA. The dysbiosis of gut microbiota indicated by the mean diversity(Shannon index) and mean similarity(Sorenson index) was severe as the liver cirrhosis developed, and AS supplementation had an apparent intervention effect on the dysbiosis of gu

关键词： Hepatic cirrhosis Gut microbiota Bacterial translocation Artesunate Intervention

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Recent Advances in Artificial Sensory Neurons:Biological fundamentals,Devices,Applications,and Challenges

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Nano-Micro Letters 2025年第3期17卷 168-216页

作者： Shuai zhong Lirou Su Mingkun Xu Desmond Loke Bin Yu Yishu Zhang Rong zhao Guangdong Institute of Intelligence Science and Technology HengqinZhuhai 519031People’s Republic of China College of Integrated Circuits Zhejiang UniversityHangzhou 3112000People’s Republic of China ZJU-Hangzhou Global Scientific and Technological Innovation Center Hangzhou 310027People’s Republic of China Department of Science Mathematics and TechnologySingapore University of Technology and DesignSingapore 487372Singapore Department of Precision Instruments Tsinghua UniversityBeijing 100084People’s Republic of China Center for Brain‑Inspired Computing Research Tsinghua UniversityBeijing 100084People’s Republic of China IDG/McGovern Institute for Brain Research Tsinghua UniversityBeijing 100084People’s Republic of China

Spike-based neural networks,which use spikes or action potentialsto represent information,have gained a lot of attention because of their high energyefficiency and low power *** fully leverage its advantages,convertingthe external analog signals to spikes is an essential *** including analog-to-digital converters or ring oscillators,and sensorssuffer from high power and area *** efforts are devoted to constructingartificial sensory neurons based on emerging devices inspired by the biologicalsensory *** can simultaneously perform sensing and spike conversion,overcoming the deficiencies of traditional sensory *** review summarizesand benchmarks the recent progress of artificial sensory *** starts with thepresentation of various mechanisms of biological signal transduction,followed bythe systematic introduction of the emerging devices employed for artificial ***,the implementations with different perceptual capabilitiesare briefly outlined and the key metrics and potential applications are also ***,we highlight the challenges and perspectives for the future development of artificial sensory neurons.

关键词： Artificial intelligence Emerging devices Artificial sensory neurons Spiking neural networks Neuromorphic sensing

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面向并行空间连接的两轮映射数据划分方法

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浙江大学学报（工学版） 2017年第1期51卷 212-224页

作者：付仲良赵星源王楠杨元维武汉大学遥感信息工程学院湖北武汉430079 地球空间信息技术协同创新中心湖北武汉430079 中国科学院遥感与数字地球研究所北京100094

针对数据划分结果高冗余、低均衡可能会增加系统的工作负荷和影响系统的负载均衡这一问题,提出两轮映射数据划分方法.在第一轮映射中,通过充分利用划分对象的空间属性来减少冗余数据,通过合理设置阈值来均衡划分数据;在第二轮映射中,通... 详细信息

针对数据划分结果高冗余、低均衡可能会增加系统的工作负荷和影响系统的负载均衡这一问题,提出两轮映射数据划分方法.在第一轮映射中,通过充分利用划分对象的空间属性来减少冗余数据,通过合理设置阈值来均衡划分数据;在第二轮映射中,通过动态映射机制,提高划分结果的数据量均衡度.与Oracle Spatial数据划分方法、线性编码轮询调度划分方法以及Hilbert编码轮询调度划分方法进行比较可知,采用两轮映射方法可以有效地控制冗余数据的产生,大幅提高划分结果的数据量均衡度,具备较好的划分效率.

关键词：地理信息系统空间数据划分空间连接

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Dual‑Donor‑Induced Crystallinity Modulation Enables 19.23% Efficiency Organic Solar Cells

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Nano-Micro Letters 2025年第3期17卷 376-388页

作者： Anhai Liang Yuqing Sun Sein Chung Jiyeong Shin Kangbo Sun Chaofeng Zhu Jingjing zhao Zhenmin zhao Yufei zhong Guangye Zhang Kilwon Cho Zhipeng Kan Center On Nanoenergy Research Institute of Science and Technology for Carbon Peak&NeutralitySchool of Physical Science&TechnologyGuangxi UniversityNanning 530004People’s Republic of China Department of Chemical Engineering Pohang University of Science and TechnologyPohang 37673South Korea College of New Materials and New Energies Shenzhen Technology UniversityShenzhen 518118People’s Republic of China Zhejiang Engineering Research Center for Fabrication and Application of Advanced Photovoltaic Materials School of Materials Science and EngineeringNingboTech UniversityNingbo 315100People’s Republic of China State Key Laboratory of Featured Metal Materials and Life-Cycle Safety for Composite Structures Nanning 530004People’s Republic of China

Trap-assisted charge recombination is one of the primary limitationsof restricting the performance of organic solar cells. However, effectivelyreducing the presence of traps in the photoactive layer remains ***, wide bandgap polymer donor PTzBI-dF is demonstrated as an effectivemodulator for enhancing the crystallinity of the bulk heterojunction active layerscomposed of D18 derivatives blended with Y6, leading to dense and orderedmolecular packings, and thus, improves photoluminescence quenching *** a result, the photovoltaic devices exhibit reduced trap-assisted charge recombinationlosses, achieving an optimized power conversion efficiency of over 19%.Besides the efficiency enhancement, the devices comprised of PTzBI-dF as athird component simultaneously attain decreased current leakage, improved chargecarrier mobilities, and suppressed bimolecular charge recombination, leading toreduced energy losses. The advanced crystalline structures induced by PTzBI-dFand its characteristics, such as well-aligned energy level, and complementaryabsorption spectra, are ascribed to the promising performance *** findings suggest that donor phase engineering is a feasible approach to tuning the molecular packings in the active layer, providingguidelines for designing effective morphology modulators for high-performance organic solar cells.

关键词： Trap-assisted charge recombination Photoluminescence Miscibility Current leakage Power conversion efficiency

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垃圾焚烧发电厂臭气控制措施的可行性分析

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化工管理 2021年第35期 49-50页

作者：仲照福北京华源惠众环保科技有限公司北京100043

生活垃圾中有机物的腐烂分解,不可避免地将产生恶臭污染。因中国人口众多,每天都会产生很多生活垃圾,为保证居住环境的干净整洁,需要做好焚烧处理,也可用于发电,在节约能源的同时创造一定的经济效益。但是生活垃圾焚烧发电厂若处理不到... 详细信息

生活垃圾中有机物的腐烂分解,不可避免地将产生恶臭污染。因中国人口众多,每天都会产生很多生活垃圾,为保证居住环境的干净整洁,需要做好焚烧处理,也可用于发电,在节约能源的同时创造一定的经济效益。但是生活垃圾焚烧发电厂若处理不到位会产生一定的臭气,若不及时处理,会对周围环境产生一定的负面影响。为解决垃圾焚烧发电厂臭气问题,文章对垃圾焚烧发电厂臭气控制措施可行性进行研究,提出垃圾焚烧发电厂臭气控制解决措施。

关键词：生活垃圾焚烧臭气控制

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