检索结果-南通市图书馆

Active DNA demethylation in plants:20 years of discovery and beyond

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2022年第12期64卷 2217-2239页

作者： Heng Zhang zhizhong gong Jian-Kang Zhu State Key Laboratory of Molecular Plant Genetics Shanghai Centre for Plant Stress BiologyCentre for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai200032 China State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing100193 China School of Life Sciences Institute of Life Science and Green DevelopmentHebei UniversityBaoding071002 China School of Life Sciences Institute of Advanced BiotechnologySouthern University of Science and TechnologyShenzhen518055 China

Maintaining proper DNA methylation levels in the genome requires active demethylation of DNA.However,removing the methyl group from a modified cytosine is chemically difficult and therefore,the underlying mechanism of demethylation had remained unclear for many years.The discovery of the first eukaryotic DNA demethylase,Arabidopsis thaliana REPRESSOR OF SILENCING 1(ROS1),led to elucidation of the 5-methylcytosine base excision repair mechanism of active DNA demethylation.In the 20 years since ROS1 was discovered,our understanding of this active DNA demethylation pathway,as well as its regulation and biological functions in plants,has greatly expanded.These exciting developments have laid the groundwork for further dissecting the regulatory mechanisms of active DNA demethylation,with potential applications in epigenome editing to facilitate crop breeding and gene therapy.

关键词： abiotic stress response base excision repair DNA demethylation DNA methylation histone modification plant growth and development plant microbe interaction

Celebrating the discovery of DNA demethylase

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2022年第12期64卷 2215-2216页

作者： zhizhong gong Jian-Kang Zhu State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBejing 100193China School of Life Sciences Institute of Life Science and Green DevelopmentHebei UniversityBaoding 071002China School of Life Sciences Institute of Advanced BiotechnologySouthern University of Science and TechnologyShenzhen 518055China

Maintaining correct DNA methylation patterns entails the addition of methyl groups by DNA methyltransferases and the active removal of methylation from DNA.Removing a methyl group from 5-methylcytosine requires breaking a strong C–C bond,suggesting that demethylation might occur by an alternative mechanism that does not involve severing this bond.Indeed,the discovery of the 5-methylcytosine DNA glycosylase(also known as DNA demethylase)REPRESSOR OF SILENCING 1(ROS1)by(gong et al.,2002)revolutionized thinking in this field.

关键词： breaking discovery

Flowering phenology as a core domestication trait in soybean

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2020年第5期62卷 546-549页

作者： zhizhong gong State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China

Flowering time variation in soybean is well characterized within domesticated germplasms and is critical for modern production,but its importance during domestication is unclear.Recently,Lu et al.(Nature Genetics,2020)reported that two homeologous pseudo-response-regulator genes,Tof12 and Tof11,were sequentially selected in early soybean evolution for ancient flowering time adaptation and intensifcation of crop cultivation.

关键词： soybean sequentially cultivation

BAK1 plays contrasting roles in regulating abscisic acid-induced stomatal closure and abscisic acid-inhibited primary root growth in Arabidopsis

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2022年第6期64卷 1264-1280页

作者： Jinping Deng Lingyao Kong Yinhua Zhu Dan Pei Xuexue Chen Yu Wang Junsheng Qi Chunpeng Song Shuhua Yang zhizhong gong State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China College of Life Sciences Qingdao UniversityQingdao 266071China Collaborative Innovation Center of Crop Stress Biology Institute of Plant Stress BiologyHenan UniversityKaifeng 475001HenanChina Institute of Life Science and Green Development School of Life SciencesHebei UniversityBaoding 071002China

The mechanisms that balance plant growth and stress responses are poorly understood, but they appear to involve abscisic acid(ABA) signaling mediated by protein kinases. Here, to explore these mechanisms, we examined the responses of Arabidopsis thaliana protein kinase mutants to ABA treatment. We found that mutants of BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED RECEPTOR KINASE 1(BAK1) were hypersensitive to the effects of ABA on both seed germination and primary root growth. The kinase OPEN STOMATA 1(OST1) was more highly activated by ABA in bak1 mutant than the wild type. BAK1 was not activated by ABA treatment in the dominant negative mutant abi1-1 or the pyr1 pyl4 pyl5 pyl8 quadruple mutant, but it was more highly activated by this treatment in the abi1-2 abi2-2 hab1-1 loss-of-function triple mutant than the wild type. BAK1 phosphorylates OST1 T146 and inhibits its activity. Genetic analyses suggested that BAK1 acts at or upstream of core components in the ABA signaling pathway, including PYLs, PP2 Cs,and Sn RK2 s, during seed germination and primary root growth. Although the upstream brassinosteroid(BR) signaling components BAK1 and BR INSENSITIVE 1(BRI1) positively regulate ABAinduced stomatal closure, mutations affecting downstream components of BR signaling, including BRASSINOSTEROID-SIGNALING KINASEs(BSKs)and BRASSINOSTEROID-INSENSITIVE 2(BIN2), did not affect ABA-mediated stomatal movement. Thus,our study uncovered an important role of BAK1 in negatively regulating ABA signaling during seed germination and primary root growth, but positively modulating ABA-induced stomatal closure, thus optimizing the plant growth under drought stress.

关键词： abscisic acid BAK1 primary root growth stomatai closure

A SUVH-DNAJ/SDJ protein complex activates the expression of promoter-methylated genes in Arabidopsis

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2019年第2期61卷 90-92页

作者： zhizhong gong State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural University

DNA methylation is an epigenetic mark that is involved in regulation of gene expression,gene imprinting,genome stability and many other important biological processes(Zhu 2009;He et al.2011;Zhang et al.2018).DNA methylation mark is usually associated with gene silencing when occurring in promoter regions.In plants,DNA methylation can be established and maintained through different DNA methylation pathways,such as the RNA-directed DNA methylation(RdDM)pathway.

关键词： DNA

RAF22,ABI1 and OST1 form a dynamic interactive network that optimizes plant growth and responses to drought stress in Arabidopsis

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2022年第7期15卷 1192-1210页

作者： Zhihui Sun Zhenkai Feng Yanglin Ding Yuanpeng Qi Shan Jiang Zhen Li Yu Wang Junsheng Qi Chunpeng Song Shuhua Yang zhizhong gong State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Collaborative Innovation Center of Crop Stress Biology Institute of Plant Stress BiologyHenan UniversityKaifeng 475001China School of Life Sciences Institute of Life Science and Green DevelopmentHebei UniversityBaoding 100193China

Plants adapt to their ever-changing environment via positive and negative signals induced by environmental stimuli.Drought stress,for instance,induces accumulation of the plant hormone abscisic acid(ABA),triggering ABA signal transduction.However,the molecular mechanisms for switching between plant growth promotion and stress response remain poorly understood.Here we report that RAF(rapidly accelerated fibrosarcoma)-LIKE MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 22(RAF22)in Arabidopsis tha/iana physically interacts with ABA INSENSITIVE 1(ABl1)and phosphorylates ABl1 at Ser416 residue to enhance its phosphatase activity.Interestingly,ABl1 can also enhance the activity of RAF22 through dephosphorylation,reciprocally inhibiting ABA signaling and promoting the maintenance of plant growth under normal conditions.Under drought stress,however,the ASA-activated OPEN STOMATA1(OST1)phosphorylates the Ser81 residue of RAF22 and inhibits its kinase activity,restraining its enhancement of ABl1 activity.Taken together,our study reveals that RAF22,ABl1,and OST1 form a dynamic regulatory network that plays crucial roles in optimizing plant growth and environmental adaptation under drought stress.

关键词： ABA signaling RAF22 ABl1 OST1 phosphorylation

Plant abiotic stress: New insights into the factors that activate and modulate plant responses

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2021年第3期63卷 429-430页

作者： zhizhong gong State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural University Beijing 100193China

Plants experience a remarkable range of interactions with the abiotic factors in their environments, both aboveground (light, temperature, mechanical stress) and belowground (soil moisture, nutrients, and mechanical properties). Plants’ abilities to sense diverse environmental parameters and initiate signaling pathways that activate precise responses have crucial implications for their survival. Plant abiotic interactions also remain a fascinating area of research, providing basic insight into plant biology and enabling efforts to improve crop plants.

关键词： plant Plant moisture

AtMCM10 promotes DNA replication-coupled nucleosome assembly in Arabidopsis

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2023年第1期65卷 203-222页

作者： Xinjie Zhao Jingyi Wang Dan Jin Jinkui Cheng Hui Chen Zhen Li Yu Wang Huiqiang Lou Jian-Kang Zhu Xuan Du zhizhong gong State Key Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural UniversityBeijing 100193 China National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences The Chinese Academy of Agricultural SciencesBeijing 100081 China State Key Laboratory of Agro-Biotechnology College of Biological Sciences China Agricultural UniversityBeijing 100193 China Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes Institute of Plant and Food ScienceSchool of Life ScienceDepartment of Biology Southern University of Science and TechnologyShenzhen 518055 China Department of Biochemistry and Molecular Biology International Cancer Center Shenzhen University Medical SchoolShenzhen 518060 China Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging National-Regional Key Technology Engineering Laboratory for Medical UltrasoundSchool of Biomedical Engineering Shenzhen University Medical SchoolShenzhen 518060 China School of Life Science Institute of Life Science and Green Development Hebei UniversityBaoding 071002 China

Minichromosome Maintenance protein 10(MCM10)is essential for DNA replication initiation and DNA elongation in yeasts and animals.Although the functions of MCM10 in DNA replication and repair have been well documented,the detailed mechanisms for MCM10 in these processes are not well known.Here,we identified AtMCM10 gene through a forward genetic screening for releasing a silenced marker gene.Although plant MCM10 possesses a similar crystal structure as animal MCM10,AtMCM10 is not essential for plant growth or development in Arabidopsis.AtMCM10 can directly bind to histone H3-H4 and promotes nucleosome assembly in vitro.The nucleosome density is decreased in Atmcm10,and most of the nucleosome density decreased regions in Atmcm10 are also regulated by newly synthesized histone chaperone Chromatin Assembly Factor-1(CAF-1).Loss of both AtMCM10 and CAF-1 is embryo lethal,indicating that AtM CM10 and CAF-1 are indispensable for replication-coupled nucleosome assembly.AtMCM10 interacts with both new and parental histones.Atmcm10 mutants have lower H3.1abundance and reduced H3K27me1/3 levels with releasing some silenced transposons.We propose that AtM CM10 deposits new and parental histones during nucleosome assembly,maintaining proper epigenetic modifications and genome stability during DNA replication.

关键词： AtMCM10 H3K27me1 nucleosomes assembly

ARF2 coordinates with PLETHORAs and PINs to orchestrate ABA-mediated root meristem activity in Arabidopsis

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2017年第1期59卷 30-43页

作者： Sujittra Promchuea Yujuan Zhu zhizhong Chen Jing Zhang zhizhong gong State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China National Center for Plant Gene Research Beijing 100193China

Multiple hormones, including abscisic acid（ABA） and auxin, regulate cell division and differentiation of Arabidopsis root meristems. AUXIN RESPONSE FACTOR2（ARF2） functions as a negative regulator of ABA responses, as seed germination and primary root growth of arf2 mutants are hypersensitive to ABA. In this study, we found that ABA treatment reduced the expression levels of the PIN-FORMEDs（PIN） auxin efflux carriers, PIN1, PIN3,PIN4, and PIN7, to a greater extent in the root meristems of arf2-101 mutant than in the wild type. Also, arf2-101 pin1 and arf2-101 pin4 double mutants show less ABA-induced inhibition of root meristem activity than the arf2-101 mutants. Furthermore, ARF2 positively mediates the transcripts of transcription factor PLETHORA 1（PLT1）gene but negatively mediates PLT2 at protein level in root meristems. Using a dexamethasone（DEX）-inducible transgenic line, Pro35S：PLT2-GR, we showed that PLT2 greatly promotes cell division and completely inhibits cell differentiation in root meristems of the arf2-101 mutant once PLT2 is induced by DEX, which can be partially reversed by ABA treatment, suggesting that ABA regulates root meristem activity in both ARF2-dependent and independent pathways. Our results uncover a complex regulatory architecture in which ARF2 coordinates with PLTs and PINs to orchestrate ABA-mediated regulation of root meristem activity in Arabidopsis.

关键词： Arabidopsis coordinates mutant auxin hypersensitive transgenic transcripts germination partially regulate