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Genome-Wide Characterization of the Hyaluronidase Gene Family and Their Potential Roles in Viviparous Black Rockfish(Sebastes schlegeli)

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Journal of Ocean University of China 2024年第2期23卷 487-498页

作者： LIU zhiying NIU Jingning ZHAO Xi LIU Huaxiang LI Zibin HE yan QI Jie MOE Key Laboratory of Marine Genetics and Breeding College of Marine Life SciencesOcean University of ChinaQingdao 266003China Key Laboratory of Tropical Aquatic Germplasm of Hainan Province Sanya Oceanographic InstituteOcean University of ChinaSanya 572025China

Black rockfish(Sebastes schlegelii)has evolved viviparous reproduction *** from the oviparous fishes,the spermatozoa of black rockfish are stored on the surface of ovarian follicle for over four months until the egg mature in ovary,while the adaptive evolution of fertilization-related genes remains to be *** the present study,based on the genome and transcriptome information of black rockfish,a total of 10 hyaluronidase genes were identified by phylogenetic and sequence analyses,including hyal1,hyal2,hyal3,hyal4,hyal6 and spam1a,spam1b,spam1c and *** spam1 subfamilies,including spam1a,spam1b,spam1c and spam1d,were significantly expanded compared to other *** adaptive evolution of hyaluronidase was further investigated by selection pressure analysis of branch model and branch site *** results showed that only spam1 subfamily was positively selected with a large number of positive selection sites,and the evolution rate was significantly higher thanthose of other *** positively selected sites,LYS-171 and GLY-164,were located in the neutral hyaluronidase activity domain,which was pivotal for SPAM1 to participate in enzymatic hydrolysis of the extracellular hyaluronic acid matrix and mediate acrosome reaction in ***,spam1 was mainly expressed in spermatocytes,secondary spermatocytes,and spermatids of testis,whereas it was not found in metamorphic sperm and mature sperm based on the spatiotemporal expression *** results indicated that spam1 might originate from the testis and might be an adaptation of viviparous trait in black rockfish.

关键词： adaptation of viviparous black rockfish(Sebastes schlegelii) hyaluronidase selective pressure analysis

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使用湖羊肉用多羔快长系优化传统湖羊性能

现代畜牧科技 2024年第6期 79-81页

作者：颜芷莹冯琳婧王笑语王承丽谢刚毅黄栢榕岳万福浙江农林大学动物科技学院·动物医学院浙江杭州311300 浙江省遂昌县喜羊羊养殖有限公司浙江遂昌323300

该文旨在评估肉用多羔快长新品系对普通湖羊在生长性状上的改造成果,通过对3、5、7月龄的本土缙云羊,庞大湖羊×缙云羊的杂交一代的体重、体长、胸围、管围进行测量,得出杂交对提高湖羊后代的生长性状的影响;杂交后代相较于本土羊在各... 详细信息

该文旨在评估肉用多羔快长新品系对普通湖羊在生长性状上的改造成果,通过对3、5、7月龄的本土缙云羊,庞大湖羊×缙云羊的杂交一代的体重、体长、胸围、管围进行测量,得出杂交对提高湖羊后代的生长性状的影响;杂交后代相较于本土羊在各方面都有所提升,各指数之间呈正相关。优质湖羊种羊进行杂交可以有效提高传统湖羊生长性能,为羊场带来更高的经济收益。

关键词：湖羊性状生长发育

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Biogenic C-doped titania templated by cyanobacteria for visible-light photocatalytic degradation of Rhodamine B

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Journal of Environmental Sciences 2014年第5期26卷 1195-1202页

作者： Jiao He Guoli Zi zhiying yan Yongli Li Jiao Xie Deliang Duan Yongjuan Chen Jiaqiang Wang The Universities' Center for Photocatalytic Treatment of Pollutants in Yunnan Province Key Laboratory of Medicinal Chemistry for Natural Resources Ministry of Education School of Chemical Sciences & Technology Yunnan University

Cyanobacteria, which occurred in eutrophic water harvest solar light to carry out photosynthesis with high efficiency. In this work, cyanobacteria （Microcystis sp.） were used as biotemplate to synthesize titania structure. The synthesized titania sample had similar morphology to that of the original template in spite of the fragile unicellular structures and extremely high water content of cyanobacterial cells. Incorporation of biogenic C, as well as the morphology inherited from biotemplate improved visible- light absorbance of the titania structure. The sample exhibited higher visible-light photocatalytic activity than commercial titania photocatalyst Degussa P25 for Rhodamine B （RhB） degradation. Compared with those C-doped titania photocatalysts prepared by other methods, cyanobacteria templated titania photocatalyst offer some potential for competitive advantages. The reported strategy opened up a new use for the cyanobacteria. It could also be used for titania in applications such as treatment of polluted water, dye-sensitized solar cells, or other regions.

关键词： cyanobacteria titania C-doped visible-light photocatalysis

Pyrotinib and chrysin synergistically potentiate autophagy in HER2-positive breast cancer

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Signal Transduction and Targeted Therapy 2024年第1期9卷 295-307页

作者： Xiaoxiao Liu Xing Zhang zhiying Shao Xiaorong Zhong Xin Ding Liang Wu Jie Chen Ping He yan Cheng Kunrui Zhu Dan Zheng Jing Jing Ting Luo Institute for Breast Health Medicine Cancer CenterBreast CenterWest China HospitalSichuan University610041 ChengduSichuanChina Department of Radiation Oncology Cancer CenterAffiliated Hospital of Xuzhou Medical UniversityJiangsu Center for the Collaboration and Innovation of Cancer BiotherapyCancer InstituteXuzhou Medical University221000 XuzhouChina Department of Orthopedics Trauma and Reconstructive SurgeryUniversity Hospital RWTH AachenAachen 52074Germany Cancer Institute Xuzhou Medical University221000 XuzhouJiangsuChina Division of Nephrology and Transplantation Department of Internal MedicineUniversity Medical Center Rotterdam Erasmus MCRotterdam 3015 GDThe Netherlands Institute for Breast Health Medicine Department of General SurgeryBreast CenterWest China HospitalSichuan University610041 ChengduSichuanChina Institute for Breast Health Medicine West China HospitalSichuan University610041 ChengduSichuanChina

Human epidermal growth factor receptor 2(HER2)-positive breast cancer(BC)has been the most challenging subtype of BC,consisting of 20%of BC with an apparent correlation with poor *** that pyrotinib,a new HER2 inhibitor,has led to dramatic improvements in prognosis,the efficacy of pyrotinib monotherapy remains largely restricted due to its acquired ***,identifying a new potential antitumor drug in combination with pyrotinib to amplify therapeutic efficacy is a pressing ***,we reported a novel combination of pyrotinib with chrysin and explored its antitumor efficacy and the underlying mechanism in HER2-positive *** determined that pyrotinib combined with chrysin yielded a potent synergistic effect to induce more evident cell cycle arrest,inhibit the proliferation of BT-474 and SK-BR-3 BC cells,and repress in vivo tumor growth in xenograft mice *** may be attributed to enhanced autophagy induced by endoplasmic reticulum ***,the combined treatment of pyrotinib and chrysin induced ubiquitination and glucose-6-phosphate dehydrogenase(G6PD)degradation by upregulating zinc finger and BTB/POZ domain-containing family protein 16(ZBTB16)in tumorigenesis of ***,we identified that miR-16-5p was a potential upstream regulator of ZBTB16,and it showed a significant inverse correlation with *** of miR-16-5p overexpression by restoring ZBTB16 significantly potentiated the overall antitumor efficacy of pyrotinib combined with chrysin against HER2-positive ***,these findings demonstrate that the combined treatment of pyrotinib and chrysin enhances autophagy in HER2-positive BC through an unrecognized miR-16-5p/ZBTB16/G6PD axis.

关键词： HER2 synergistic Together

Enhanced methane production in an anaerobic digestion and microbial electrolysis cell coupled system with co-cultivation of Geobacter and Methanosarcina

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Journal of Environmental Sciences 2016年第4期28卷 210-214页

作者： Qi Yin Xiaoyu Zhu Guoqiang Zhan Tao Bo yanfei yang Yong Tao Xiaohong He Daping Li zhiying yan Key Laboratory of Environmental and Applied Microbiology Chengdu Institute of Biology Chinese Academy of Sciences Chengdu 610041 China Environmental Microbiology Key Laboratory of Sichuan Province Chengdu 610041 China

The anaerobic digestion（AD）and microbial electrolysis cell（MEC）coupled system has been proved to be a promising process for biomethane *** this paper,it was found that by co-cultivating Geobacter with Methanosarcina in an AD–MEC coupled system,methane yield was further increased by 24.1%,achieving to 360.2 m L/g-COD,which was comparable to the theoretical methane yield of an anaerobic *** the presence of Geobacter,the maximum chemical oxygen demand（COD）removal rate（216.8 mg COD/（L·hr））and current density（304.3 A/m3）were both increased by 1.3 and 1.8 fold compared to the previous study without Geobacter,resulting in overall energy efficiency reaching up to 74.6%.Community analysis demonstrated that Geobacter and Methanosarcina could coexist together in the biofilm,and the electrochemical activities of both were confirmed by cyclic *** study observed that the carbon dioxide content in total gas generated from the AD reactor with Geobacter was only half of that generated from the same reactor without Geobacter,suggesting that Methanosarcina may obtain the electron transferred from Geobacter for the reduction of carbon dioxide to *** together,Geobacter not only can improve the performance of the MEC system,but also can enhance methane production.

关键词： AD–MEC coupled system Geobacter Methanosarcina Co-cultivation Methane production

Impact of Blade-Flapping Vibration on Aerodynamic Characteristics of Wind Turbines under Yaw Conditions

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Energy Engineering 2024年第8期121卷 2213-2229页

作者： Shaokun Liu zhiying Gao Rina Su Mengmeng yan Jianwen Wang College of Energy and Power Engineering Inner Mongolia University of TechnologyHohhot010051China Key Laboratory of Wind and Solar Energy Utilization Technology Ministry of EducationHohhot010051China

Although the aerodynamic loading of wind turbine blades under various conditions has been widely studied,the radial distribution of load along the blade under various yaw conditions and with blade flapping phenomena is poorly *** study aims to investigate the effects of second-order flapwise vibration on the mean and fluctuation characteristics of the torque and axial thrust of wind turbines under yaw conditions using computational fluid dynamics(CFD).In the CFD model,the blades are segmented radially to comprehensively analyze the distribution patterns of torque,axial load,and tangential *** following results are obtained.(i)After applying flapwise vibration,the torque and axial thrust of wind turbines decrease in relation to those of the rigid model,with significantly increased fluctuations.(ii)Flapwise vibration causes the blades to reciprocate along the axial direction,altering the local angle of attack and velocity of the blades relative to the incoming wind *** results in the contraction of the torque region from a circular shape to a complex“gear”shape,which is accompanied by evident oscillations.(iii)Compared to the tangential load,the axial load on the blades is more sensitive to flapwise vibration although both exhibit significantly enhanced *** study not only reveals the impact of flapwise vibration on wind turbine blade performance,including the reduction of torque and axial thrust and increased operational fluctuations,but also clarifies the radial distribution patterns of blade aerodynamic characteristics,which is of great significance for optimizing wind turbine blade design and reducing fatigue risks.

关键词： Wind turbine CFD numerical simulation aerodynamic characteristics yaw flapping vibration

基于柠檬酸处理的枸杞枝条发酵饲料对山羊生长、肉质的影响及其微生物群落

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应用与环境生物学报 2023年第1期29卷 70-78页

作者：刘杨康宇丁悦安巍王亚军闫志英中国科学院环境与应用微生物重点实验室环境微生物四川省重点实验室中国科学院成都生物研究所成都610041 宁夏农林科学院枸杞科学研究所银川750002 成都大学食品与生物工程学院成都610106

枸杞枝条(Lycium barbarum branches,LBB)的资源化处理是枸杞循环种植的重要组成部分.为探究枸杞枝条发酵饲料的可行性,为枸杞枝条的饲料化推广和应用提供理论依据和实践指导,开展了枸杞枝条预处理实验、发酵实验和山羊饲喂评价试验.结... 详细信息

枸杞枝条(Lycium barbarum branches,LBB)的资源化处理是枸杞循环种植的重要组成部分.为探究枸杞枝条发酵饲料的可行性,为枸杞枝条的饲料化推广和应用提供理论依据和实践指导,开展了枸杞枝条预处理实验、发酵实验和山羊饲喂评价试验.结果显示2‰柠檬酸预处理枸杞枝条36 h,枝条中纤维素、木质素、半纤维素降解率达19.67%、16.48%、47.04%,预处理效果显著.发酵25 d后,产物中乳酸含量达28.63 mg/g、乙酸含量达6.87 mg/g,乳酸菌活菌数达3.90×107cfu/g,酵母菌活菌数达2.40×105cfu/g,枸杞多糖含量为3.11%,枝条发酵品质和饲用价值得到提升.山羊饲喂实验结果显示10%为枸杞枝条发酵饲料最佳添加比例,山羊采食量达0.80 kg/(只·天),山羊月增重为10.08 kg只,较空白组均显著提升(P<0.05).枸杞枝条发酵饲料对山羊的肉质无显著影响,且能增加山羊的肠道中功能微生物——瘤胃球菌属Ruminococcus丰度,促进山羊肠道对营养物质的吸收.本研究表明经过柠檬酸预处理后的枸杞枝条能发酵成为饲料,可促进山羊的采食和增长且对其肉质无影响.(图5表6参30)

关键词：枸杞枝条山羊饲喂生理特性肉质评价微生物分析

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非遗传因素提高湖羊肉品质的途径和机理初探

现代畜牧科技 2024年第3期 55-58页

作者：冯琳婧颜芷莹王笑语吴攀王承丽岳万福浙江农林大学动物科技学院·动物医学院浙江杭州311300

湖羊以其优良适应性,服务于我国畜牧业结构调整,遗传选择和精细营养管理被广泛用于提高湖羊的生长速度和产量。湖羊产羔数稳定提高,生长速度和体质也得到提升,但瘦肉率提升幅度不大。该文试图通过对湖羊养殖场的实地调查与资料分析,解... 详细信息

湖羊以其优良适应性,服务于我国畜牧业结构调整,遗传选择和精细营养管理被广泛用于提高湖羊的生长速度和产量。湖羊产羔数稳定提高,生长速度和体质也得到提升,但瘦肉率提升幅度不大。该文试图通过对湖羊养殖场的实地调查与资料分析,解析非遗传因素提升湖羊瘦肉率的方法和途径。

关键词：断奶成活率产羔数 7月龄体重瘦肉率维生素A

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一种波特率自动校准的通用异步收发器设计

集成电路应用 2023年第1期40卷 26-28页

作者：颜志颖戴显英西安电子科技大学广州研究院广东510555 西安电子科技大学微电子学院陕西710071

阐述基于硬件描述语言设计了一个波特率自动校准的通用异步收发器。采用硬件法实时监测接收端波特率并更新分频系数,实现对接收波特率的自动校准。采用验证方法学对模块整体进行仿真验证,回归测试表明该设计可以实现数据串并转换功能,... 详细信息

阐述基于硬件描述语言设计了一个波特率自动校准的通用异步收发器。采用硬件法实时监测接收端波特率并更新分频系数,实现对接收波特率的自动校准。采用验证方法学对模块整体进行仿真验证,回归测试表明该设计可以实现数据串并转换功能,并且在收发双方波特率存在一定误差时,可以有效抑制接收数据时采样点的偏移。相比于传统异步收发器,可以在非标准波特率场景下更准确地采样数据。

关键词：通用异步收发器波特率自动校准