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一种新型电化学免疫传感器检测血清4型禽腺病毒

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中国兽医学报 2024年第2期44卷 249-257页

作者：黄娇玲谢芝勋罗思思李孟谢丽基邓显文谢志勤曾婷婷张艳芳范晴张民秀王盛李丹韦悠李小凤广西壮族自治区兽医研究所农业农村部中国(广西)-东盟跨境动物疫病防控重点实验室/广西兽医生物技术重点实验室广西南宁530001

将多壁碳纳米管-壳聚糖-铂纳米粒子复合材料(MWCNT-Chi-PtNP)修饰金电极(GE),并吸附血清4型禽腺病毒(fowl adenovirus serotype 4,FAdV-4)蛋白单克隆抗体(MAb/FAdV-4),构建一种新型的电阻型电化学免疫传感器用于检测FAdV-4。对传感器的... 详细信息

将多壁碳纳米管-壳聚糖-铂纳米粒子复合材料(MWCNT-Chi-PtNP)修饰金电极(GE),并吸附血清4型禽腺病毒(fowl adenovirus serotype 4,FAdV-4)蛋白单克隆抗体(MAb/FAdV-4),构建一种新型的电阻型电化学免疫传感器用于检测FAdV-4。对传感器的测试底液及样品孵育时间进行优化后,在最优条件下,对所构建的传感器的敏感性、特异性和稳定性进行检测。并对36份临床样品进行检测,以验证该传感器的实用性。结果显示,所建立的FAdV-4电化学免疫传感器的测试底液最佳pH值为7.0,样品的最佳孵育时间为30 min,检测线性范围10^(1.46)~10^(4.46)EID50/mL FAdV-4,最低检出限为10^(1.89)EID50/mL FAdV-4(S/N=3);敏感性试验结果显示该免疫传感器灵敏度比常规PCR方法(10^(3.46)EID50/mL)FAdV-4高37倍,且该传感器特异性高,稳定性好;该传感器对36份临床样品进行检测,结果与PCR方法和测序结果相符。结果表明,所建立的FAdV-4电化学免疫传感器具有检测快速、特异性好和敏感性高等优点,为FAdV-4的诊断提供一种新型的快速检测技术。

关键词：多壁碳纳米管电化学免疫传感器铂纳米粒子血清4型禽腺病毒

血清4型禽腺病毒感染LMH细胞后干扰素刺激基因在转录水平表达量的动态变化

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中国畜牧兽医 2024年第5期51卷 2081-2090页

作者：万丽军王盛谢芝勋任红玉谢丽基范晴罗思思李孟张艳芳曾婷婷黄娇玲张民秀谢志勤李小凤韦悠广西壮族自治区兽医研究所广西兽医生物技术重点实验室南宁530001

【目的】了解鸡肝癌细胞(LMH)感染血清4型禽腺病毒(FAdV-4)后干扰素(IFN)和干扰素刺激基因(ISGs)的表达量变化,为研究FAdV-4与ISGs的相互作用提供参考依据。【方法】试验将FAdV-4感染LMH细胞,观察不同时间点细胞病变,并收集感染0、12、2... 详细信息

【目的】了解鸡肝癌细胞(LMH)感染血清4型禽腺病毒(FAdV-4)后干扰素(IFN)和干扰素刺激基因(ISGs)的表达量变化,为研究FAdV-4与ISGs的相互作用提供参考依据。【方法】试验将FAdV-4感染LMH细胞,观察不同时间点细胞病变,并收集感染0、12、24、36、48、60、72、84和96 h的细胞样品,通过实时荧光定量PCR技术检测感染病毒后不同时间点IFN-α和IFN-β及ISGs基因在转录水平上表达量的动态变化规律。【结果】LMH细胞感染FAdV-448 h时出现典型的细胞病变;在感染12 h后,FAdV-4快速增殖,60 h时达到峰值;感染病毒后各时间点,与对照组相比,IFN-α基因转录水平表达量均显著下调(P<0.05);与对照组相比,在感染后12和24 h,IFN-β基因表达量显著下调(P<0.05),在36 h时迅速上调表达至峰值(P<0.05),在48 h时仍维持在较高水平,之后时间点的表达量有所下降且趋于稳定,但仍显著高于对照组(P<0.05);ISG 12、IFIT 5及DDIT 4基因在感染前期变化不大,在感染后36 h时迅速上调,在感染后96 h达到峰值(P<0.05);ZFP 313、IFITM 3及Viperin基因在感染前期表达量变化不大,随后在36 h迅速上调表达至峰值(P<0.05),在48 h时仍维持在较高水平,之后时间点的表达量有所下降,但仍显著高于对照组(P<0.05);与对照组相比,CD 47、OAS和PKR基因均呈现下调表达。【结论】在FAdV-4感染LMH细胞后,IFN及多种ISGs在转录水平呈现规律性变化,与病毒在LMH细胞中复制存在一定的联系,表明这些天然免疫因子可能在抗FAdV-4反应中发挥着重要作用。

关键词：血清4型禽腺病毒(FAdV-4) LMH细胞干扰素干扰素刺激基因转录

Comparison of cancer incidence and mortality in three GDP per capita levels in China, 2013

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Chinese Journal of Cancer Research 2017年第5期29卷 385-394页

作者： zhixun Yang Rongshou Zheng Siwei Zhang Hongmei Zeng Changfa Xia He Li Li wang Yanhong wang Wanqing Chen National Office for Cancer Prevention and Control National Cancer Center/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Department of Epidemiology and Bio-statistics Institute of Basic Medical Sciences Chinese Academy of Medical Sciences & School of Basic Medicine Peking Union Medical College

Objective：In this research,the patterns of cancer incidence and mortality in areas with different gross domestic product per capita（GDPPC）levels in China were explored,using data from population-based cancer registries in 2013,collected by the National Central Cancer Registry（NCCR）.Methods：Data from 255 cancer registries were qualified and included in this *** on the GDPPC data of 2014,cities/counties were divided into 3 levels：high-,middle-and low-GDPPC areas,with 40,000 and 80,000 RMB per year as cut *** calculated cancer incidences and mortalities in these three levels,stratified by gender and age *** national population of the Fifth Census in 2000 and Segi’s population were applied for age-standardized ***：The crude incidence and mortality rates as well as age-standardized incidence rate（ASIR）showed positive associations with GDPPC *** age-standardized mortality rate（ASMR）nevertheless showed a negative association with GDPPC *** ASMR in high-,middle-and low-GDPPC areas was 103.12/100,000,112.49/100,000 and 117.43/100,000,*** cancer was by far the most common cancer in all three GDPPC *** was also the leading cause of cancer death,regardless of gender and GDPPC *** associations with GDPPC level were found for the ASIRs of lung,stomach,esophageal and liver cancer,whereas colorectal and breast cancer showed positive *** for breast cancer,the ASMRs of the other five cancers were always higher in middle-and low-GDPPC areas than in high-GDPPC ***：The economic development is one of the main factors of the heavy cancer burden on Chinese *** would be reasonable to implement cancer control strategies referring to the local GDPPC level.

关键词： Cancer incidence mortality epidemiology GDP per capita China

鸡传染性喉气管炎病毒微滴式数字PCR定量检测方法的建立

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Trends in geographical disparities for cervical cancer mortality in China from 1973 to 2013:a subnational spatio-temporal study

西北农业学报 2024年第3期33卷 381-388页

作者：谢志勤谢芝勋张艳芳范晴谢丽基万丽军罗思思李孟张民秀曾婷婷黄娇玲王盛李丹韦悠李小凤任红玉阮志华广西壮族自治区兽医研究所广西兽医生物技术重点实验室南宁 530001

旨在建立检测鸡传染性喉气管炎病毒(infectious laryngotracheitis virus,ILTV)绝对定量方法.根据已发表的鸡ILTV的TK基因序列,针对其保守区域分别设计1对特异引物和1条探针,建立检测鸡ILTV的微滴式数字PCR(droplet digital PCR,ddPCR)... 详细信息

旨在建立检测鸡传染性喉气管炎病毒(infectious laryngotracheitis virus,ILTV)绝对定量方法.根据已发表的鸡ILTV的TK基因序列,针对其保守区域分别设计1对特异引物和1条探针,建立检测鸡ILTV的微滴式数字PCR(droplet digital PCR,ddPCR)方法,并评价其特异性、敏感性和重复性.结果显示,建立方法的最佳引物浓度为20 μmol/μL,最佳探针浓度为10 μmol/μL,最佳退火温度为56 ℃.特异性检测结果显示,建立的方法只检出ILTV,没有检出其他病原株.敏感性检测结果显示,采用建立的方法定量检出ILTV重组质粒标准品的最低限为4.6拷贝/μL.对3个连续稀释的pMD18-ILTV重组质粒DNA进行检测,3次重复检测结果的变异系数均小于5％.对83份病鸡喉拭子、肺及脾组织样品进行检测,采用建立的ddPCR检出ILTV阳性样品10份,荧光定量PCR检出ILTV阳性样品9份,ddPCR的阳性检出率(12.05％)高于荧光定量PCR的阳性检出率(10.84％).结果表明,建立的ddPCR方法定量检测ILTV特异性强、敏感性高、重复性好,为绝对定量检测ILTV提供更好的技术支撑.

关键词：鸡传染性喉气管炎病毒微滴式数字PCR 定量检测

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Chinese Journal of Cancer Research 2017年第6期29卷 487-495页

作者： Changfa Xia Chao Ding Rongshou Zheng Siwei Zhang Hongmei Zeng Jinfeng wang Yilan Liao Ningxu Zhang zhixun Yang Wanqing Chen National Cancer Center/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College State Key Laboratory of Resources and Environmental Information System Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of Sciences

Objective： This study was designed to explore the time trends in geographical variations of cervical cancer mortality in China's Mainland over the period 1973 to 2013, to provide subnational spatio-temporal patterns for targeted promotion of human papillomavirus vaccine in China. Methods： Data were extracted from three national retrospective death surveys and cancer registry. The rate ratio （RR） was estimated for the aggregated data for seven geographical regions using generalized linear models to evaluate time trends in geographical disparities of cervical cancer mortality. Results： There was a significant decrease in cervical cancer mortality in China from 1973-1975 to 2004-2005, but leveled off thereafter to 2011-2013. Compared to the period 1973-1975 the RR for the three last time periods were 0.33 [95% confidence interval （95% CI）： 0.30-0.37] for 1990-1992, 0.21 （95% CI： 0.19-0.24） for 2004-2005 and 0.24 （95% CI： 0.22-0.26） for 2011-2013. Females living in the Northwest China and Central China have a high risk of mortality from cervical cancer compared to the nationwide, with the RR being 2.09 （95% CI： 1.83-2.38） and 1.26 （95% CI： t.11-1.44） respectively, while the RRs for South China, Northeast China and Southwest China were below 1.00, indicating the lower death risk. Despite the mortality rate had increased slightly from 2004 to 2013, there was an encouraging sign that the geographical disparities in cervical cancer mortality had gradually narrowed over time across China. Conclusions： Although cervical cancer mortality in China has reduced to very low levels, the high risk of cervical cancer in Northwest China and Central China is still noteworthy. Public health policies including the promotion of vaccine should be targeted to further reduction of geographical disparities in cervical cancer mortality.

关键词： Cervical cancer mortality spado-temporal epidemiology China

Safety and survival outcomes of transanal natural orifice specimen extraction using prolapsing technique for patients with middle-to low-rectal cancer

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Chinese Journal of Cancer Research 2020年第5期32卷 654-664页

作者： Zhao Lu Haipeng Chen Mingguang Zhang Xu Guan zhixun Zhao Zheng Jiang Zheng Liu Zhaoxu Zheng Xishan wang Department of Colorectal Surgery National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China

Objective:The transanal approach to specimen collection,combined with the prolapsing technique,is a wellestablished and minimally invasive surgery for treating rectal ***,reports on outcomes for this approach are *** compared short-and long-term outcomes of conventional laparoscopic surgery(CLS)*** natural orifice specimen extraction(NOSE)using the prolapsing technique for patients with middle-to low-rectal ***:From January 2013 to December 2017,we enrolled consecutive patients with middle-to low-rectal cancer undergoing laparoscopic anterior ***,50 patients who underwent transanal NOSE using the prolapsing technique were matched with 50 patients who received *** parameters and survival outcomes between the two groups were ***:Estimated blood loss(29.70±29.28 vs.52.80±45.09 mL,P=0.003),time to first flatus(2.50±0.79 vs.2.86±0.76,P=0.022),time to liquid diet(3.62±0.64 vs.4.20±0.76 d,P<0.001),and the need for analgesics(22%vs.48%,P=0.006)were significantly lower for the NOSE group compared to the CLS *** incidences of overall complications and fecal incontinence were comparable in both *** a median follow-up of 44.52 months,the overall local recurrence rate(6%vs.5%,P=0.670),3-year disease-free survival(86.7%vs.88.0%,P=0.945)and 3-year overall survival(95.6%vs.96.0%,P=0.708),were not significantly ***:For total laparoscopic rectal resection,transanal NOSE using the prolapsing technique is effective and safe,and associated with less trauma and pain,a faster recovery,and similar survival outcomes compared to CLS.

关键词： Natural orifice specimen extraction transanal specimen extraction rectal cancer prolapsing technique survival

基于“虚气留滞”理论探讨细胞自噬在桥本甲状腺炎中的作用

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吉林中医药 2024年第1期44卷 22-27页

作者：司新颖李会龙郭志卂李欣怡吴艳珂马文源耿文倩丁治国北京中医药大学第一临床医学院北京100700 北京中医药大学东直门医院甲状腺病科北京100700 北京中医药大学孙思邈医院甲状腺病科陕西铜川727100

桥本甲状腺炎(HT)中医病机以脾气亏虚为本,以气火痰瘀为标,其因虚而滞,因滞更虚的动态病机特点,可以王永炎院士“虚气留滞”学说概之。中医学认为,细胞自噬为气化的微观表现,具有清除病邪,维持内环境稳态之功。自噬水平不足可导致受损... 详细信息

桥本甲状腺炎(HT)中医病机以脾气亏虚为本,以气火痰瘀为标,其因虚而滞,因滞更虚的动态病机特点,可以王永炎院士“虚气留滞”学说概之。中医学认为,细胞自噬为气化的微观表现,具有清除病邪,维持内环境稳态之功。自噬水平不足可导致受损细胞器等废物无力清除、异常蓄积,或可看作虚气留滞病机的微观体现。本文基于“虚气留滞”理论探讨桥本甲状腺炎与细胞自噬的相关性,确立“补虚化滞”治法,以调控甲状腺滤泡上皮细胞自噬功能为治疗靶点,为中医药防治桥本甲状腺炎病变提供新思路。

关键词：桥本甲状腺炎自噬虚气留滞

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基于无人机机载AI模块的电力巡检技术

沈阳工业大学学报 2024年第1期46卷 49-53页

作者：王昊丁国斌杨家慧南方电网数字电网研究院有限公司广东广州510700 广州致讯信息科技有限责任公司研发部广东广州510700

针对输电线路无人机巡检实时性和准确性的要求,深入研究了YOLOv3目标检测算法在无人机巡检机载AI模块中的应用。利用将目标检测候选区选取和对象识别合二为一的YOLOv3算法,结合多尺度特征融合方式实现了目标检测的高准确性和实时优化,... 详细信息

针对输电线路无人机巡检实时性和准确性的要求,深入研究了YOLOv3目标检测算法在无人机巡检机载AI模块中的应用。利用将目标检测候选区选取和对象识别合二为一的YOLOv3算法,结合多尺度特征融合方式实现了目标检测的高准确性和实时优化,并采用残差块解决了模型退化问题。输电线路绝缘子检测结果表明:YOLOv3算法平均精度可达90%,相同条件下YOLOv3算法平均处理速度约为Faster RCNN算法的3.2倍,约为SSD算法的1.6倍。

关键词：无人机巡检目标检测图像识别多尺度特征融合残差块输电线路实时性

细长螺旋结构拉伸力学行为解析理论适用性研究

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中国造船 2023年第5期64卷 159-170页

作者：杨志勋王华琳王刚樊耀华阎军史冬岩哈尔滨工程大学机电工程学院船舶机械研究所哈尔滨150001 大连交通大学土木工程学院大连116028 大连理工大学车辆工程与机械学院工程力学系大连116024

基于曲梁理论推导了细长螺旋结构拉伸力学行为的求解公式,探究了不同缠绕角度结构在轴向拉力作用下的力学变形机制。通过理论解与数值解的比较,进一步明确了不同缠绕角度细长螺旋结构的变形特征和不同理论求解方法的适用范围。研究发现... 详细信息

基于曲梁理论推导了细长螺旋结构拉伸力学行为的求解公式,探究了不同缠绕角度结构在轴向拉力作用下的力学变形机制。通过理论解与数值解的比较,进一步明确了不同缠绕角度细长螺旋结构的变形特征和不同理论求解方法的适用范围。研究发现,具有较大缠绕角度的细长螺旋结构在受拉时主要产生扭转变形,而对具有较小缠绕角度的细长螺旋结构截面拉伸变形起主导作用。在相同轴向应变前提下,不同缠绕角度的细长螺旋结构内力以45°为中心成对称分布,这大大降低了工程设计的计算量,为工程中细长螺旋结构的分析设计提供了有益参考。

关键词：细长螺旋结构缠绕角度力学行为拉伸