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Synthetic and Systems Biotechnology 2024年第3期9卷 503-512页

作者： Bing Fu Meng Chen Xianfeng Bao Jiajie Lu zhiwen Zhu Fuyao Guan Chuyang yan Peize Wang Linglin Fu Ping Yu College of Food Science and Biotechnology Zhejiang Gongshang University149 Jiaogong RoadHangzhouZhejiang Province310035People’s Republic of China College of Forestry Science and Technology Lishui Vocational and Technical College357 Zhongshan Street NorthLishuiZhejiang Province323000People’s Republic of China Lishui Institute for Quality Inspection and Testing 395 Zhongshan StreetLishuiZhejiang Province323000People’s Republic of China

Vitamin B_(2)is an essential water-soluble *** most prokaryotes,a bifunctional enzyme called FAD synthase catalyzes the successive conversion of riboflavin to FMN and *** this study,the plasmid pNEW-AZ containing six key genes for the riboflavin synthesis was transformed into strain R2 with the deleted FMN riboswitch,yielding strain *** R5 strain could produce 540.23±5.40 mg/L riboflavin,which was 10.61%higher than the R4 strain containing plasmids pET-AE and pAC-Z harboring six key *** further enhance the production of riboflavin,homology matching and molecular docking were performed to identify key amino acid residues of FAD *** point mutation sites were *** comparing riboflavin kinase activity,mutations of T203D and N210D,which respectively decreased by 29.90%and 89.32%compared to wild-type FAD synthase,were selected for CRISPR/Cas9 gene editing of the genome,generating engineered strains R203 and ***-AZ was transformed into R203,generating R6.R6 produced 657.38±47.48 mg/L riboflavin,a 21.69%increase compared to *** study contributes to the high production of riboflavin in recombinant *** BL21.

关键词： Vitamin B_(2) FAD synthase Escherichia coli BL21 Riboflavin kinase CRISPR/Cas9

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大跨度桥梁脉动风场模拟的插值算法

振动与冲击 2017年第7期36卷 156-163页

作者：祝志文黄炎湖南大学土木工程学院长沙410082 湖南大学风工程与桥梁工程湖南省重点实验室长沙410082

为减少谐波合成法中功率谱矩阵分解的计算量,基于双索引频率和谱矩阵特点,提出了谱解矩阵双轴插值算法和递归插值算法。两种方法均使风场模拟计算效率大幅度提高,且得到的模拟风场均与传统谐波合成法在统计意义上相符;研究了插值节点数... 详细信息

为减少谐波合成法中功率谱矩阵分解的计算量,基于双索引频率和谱矩阵特点,提出了谱解矩阵双轴插值算法和递归插值算法。两种方法均使风场模拟计算效率大幅度提高,且得到的模拟风场均与传统谐波合成法在统计意义上相符;研究了插值节点数量及分布形式对风场模拟的影响,认为在谱解矩阵的频率轴向采取前密后疏分段插值,双索引频率轴向采用均匀分布插值节点的形式,更适合于风场模拟。通过对一实际大跨度桥梁风场的模拟,验证了方法的有效性。

关键词：风场模拟大跨度桥梁谐波合成法插值算法

设施蔬菜水肥一体化智能灌溉控制系统的设计及试验

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山西农业科学 2017年第9期45卷 1534-1538页

作者：张雪飞彭凯王建春宋治文王艳梁新书钱春阳李鹏天津市农业科学院信息研究所天津300192 河北工业大学机械学院天津300130 天津市农业资源与环境研究所天津300192 天津亿网通达网络技术有限公司天津300100

设计了设施蔬菜水肥一体化智能灌溉控制系统,该系统通过环境传感器实现对环境对象感测,精准获取设施蔬菜的全生育期的环境参数;设计了"土壤含水量下限+小额灌溉"的灌溉策略模式,结合设施蔬菜全生育期的环境参数形成闭环回路,驱动文丘里... 详细信息

设计了设施蔬菜水肥一体化智能灌溉控制系统,该系统通过环境传感器实现对环境对象感测,精准获取设施蔬菜的全生育期的环境参数;设计了"土壤含水量下限+小额灌溉"的灌溉策略模式,结合设施蔬菜全生育期的环境参数形成闭环回路,驱动文丘里比例施肥机进行有效水肥灌溉完成对设施蔬菜的精细化耕作。结果表明,该系统能够按照预设间隔时间有效监控设施蔬菜的环境参数,并能在设定的土壤湿度参数范围内启动或者浇灌,实现智能浇灌,比传统的自动浇灌系统更加高效。

关键词：水肥一体化无线传感网络环境调控设施蔬菜灌溉策略

Nanostructuring gold wires as highly durable nano- catalysts for selective reduction of nitro compounds and azides with organosilanes

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Nano Research 2015年第4期8卷 1365-1372页

作者： Huifang Guo Xiulinq yan Yun Zhi zhiwen Li Cai Wu Chunliang Zhao Jing Wang Zhixin Yu Yi Ding Wei He Yadong Li School of Medicine and Tsinghua-Peking Joint Center for Life Sciences Tsinghua University Beijing 100084 China Institute for New Energy Materials and Low-Carbon Technologies and School of Materials Science and Engineering Tianjin University of Technology Tianjin 300384 China School of Chemistry and Chemical Engineering Shandong University Jinan 250100 China Department of Petroleum Engineering University ofStavanger Stavanger 4036 Norway Department of Chemistry Tsinghua University Beijing 100084 China

A general method is developed to prepare durable hybrid nanocatalysts by nanostructuring the surface of gold wires via simple alloying and dealloying. The resulting nanoporous gold/Au （NPG/Au） wire catalysts possess nanoporous skins with their thicknesses on robust metal wires specified in a highly controllable manner. As a demonstration, the as-obtained NPG/Au was shown to be a highly active, chemo-selective, and recyclable catalyst for the reduction of nitro com- pounds and azides using organosilanes as reducing agents.

关键词： nanocatalyst gold catalysis reduction

Chromosomal microarray analysis *** for fetal ventriculomegaly:a meta-analysis

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Chinese Medical Journal 2022年第3期135卷 268-275页

作者： yan Sun Weiyuan Zhang zhiwen Wang Likui Guo Shaowen Shi Department of Perinatal Medicine Beijing Obstetrics and Gynecology HospitalCapital Medical UniversityBeijing 100010China Department of Obstetrics First Hospital of QinhuangdaoHebei 066000China

Background:Chromosomal abnormalities are important causes of ventriculomegaly(VM).In mild and isolated cases of fetal VM,obstetricians rarely give clear indications for pregnancy *** aimed to calculate the incidence of chromosomal abnormalities and incremental yield of chromosomal microarray analysis(CMA)in VM,providing more information on genetic counseling and prognostic evaluation for fetuses with ***:The Chinese language databases Wanfang Data,China National Knowledge Infrastructure,and China Biomedical Literature Database(from January 1,1991 to April 29,2020)and English language databases PubMed,Embase,and Cochrane Library(from January 1,1945 to April 29,2020)were systematically searched for articles on fetal *** criteria were based on ultrasonographic or magnetic resonance imaging(MRI)assessment of lateral ventricular atrium width:≥10 to<15 mm for mild VM,and≥15 mm for severe *** VM was defined by the absence of structural abnormalities other than VM detected by ultrasonography or MRI.R software was used for the meta-analysis to determine the incidence of chromosomal abnormalities and incremental yield of CMA in VM,and the combined rate and 95%confidence interval(CI)were ***:Twenty-three articles involving 1635 patients were *** incidence of chromosomal abnormalities in VM was 9%(95%CI:5%-12%)and incremental yield of CMA in VM was 11%(95%CI:7%-16%).The incidences of chromosomal abnormalities in mild,severe,isolated,and non-isolated VM were 9%(95%CI:4%-16%),5%(95%CI:1%-11%),3%(95%CI:1%-6%),and 13%(95%CI:4%-25%),***:Applying CMA in VM improved the detection rate of *** VM is confirmed by ultrasound or MRI,obstetricians should recommend fetal karyotype analysis to exclude chromosomal ***,CMA should be recommended preferentially in pregnant women with fetal VM who are undergoing invasive prenatal *** cannot completely replace chromosome kary

关键词： Ventriculomegaly Chromosome Karyotype Chromosomal microarray analysis Meta-analysis

Selective Hydrodeoxygenation of Lignin-Derived Vanillin via Hetero-Structured High-Entropy Alloy/Oxide Catalysts

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Energy & Environmental Materials 2024年第3期7卷 202-210页

作者： yan Sun Kaili Liang Ren Tu Xudong Fan Charles Q.Jia zhiwen Jia Yingnan Li Hui yang Enchen Jiang Hanwen Liu Yonggang Yao Xiwei Xu Key Laboratory for Biobased Materials and Energy of Ministry of Education College of Materials and EnergySouth China Agricultural UniversityGuangzhou 510640China Department of Chemical Engineering and Applied Chemistry University of Toronto200 College StreetToronto ON M5S3E5Canada State Key Laboratory of Materials Processing and Die&Mould Technology School of Materials Science and EngineeringHuazhong University of Science and TechnologyWuhan 430074China

The chemoselective hydrodeoxygenation of natural lignocellulosic materials plays a crucial role in converting biomass into value-added *** their complex molecular structures often require multiple active sites synergy for effective activation and achieving high ***,it is reported that a high-entropy alloy(HEA)on high-entropy oxide(HEO)hetero-structured catalyst for highly active,chemoselective,and robust vanillin *** heterogenous HEA/HEO catalysts were prepared by thermal reduction of senary HEOs(NiZnCuFeAlZrO_(x)),where exsolvable metals(e.g.,Ni,Zn,Cu)in situ emerged and formed randomly dispersed HEA nanoparticles anchoring on the HEO *** catalyst exhibits excellent catalytic performance:100%conversion of vanillin and 95%selectivity toward high-value 2-methyl-4 methoxy phenol at low temperature of 120℃,which were attributed to the synergistic effect among HEO matrix(with abundant oxygen vacancies),anchored HEA nanoparticles(having excellent hydrogenolysis capability),and their intimate hetero-interfaces(showing strong electron transferring effect).Therefore,our work reported the successful construction of HEA/HEO heterogeneous catalysts and their superior multifunctionality in biomass conversion,which could shed light on catalyst design for many important reactions that are complex and require multifunctional active sites.

关键词： biomass conversion heterogeneous catalysts high-entropy oxide high-entropy alloys lignin pyrolysis

Perovskite films with gradient bandgap for self-powered multiband photodetectors and spectrometers

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Nano Research 2023年第7期16卷 10256-10262页

作者： Chuantian Zuo Lixiu Zhang Xiyan Pan He Tian Keyou yan Yuanhang Cheng zhiwen Jin Chenyi Yi Xiaoliang Zhang Wu-Qiang Wu Qinye Bao Liyuan Han Liming Ding Center for Excellence in Nanoscience(CAS) Key Laboratory of Nanosystem and Hierarchical Fabrication(CAS)National Center for Nanoscience and TechnologyBeijing 100190China School of Physics and Electronic Science East China Normal UniversityShanghai 200241China School of Integrated Circuits Tsinghua UniversityBeijing 100084China School of Environment and Energy South China University of TechnologyGuangzhou 510000China School of New Energy Nanjing University of Science and TechnologyJiangyin 214443China School of Physical Science and Technology Lanzhou UniversityLanzhou 730000China State Key Laboratory of Power System Department of Electrical EngineeringTsinghua UniversityBeijing 100084China School of Materials Science and Engineering Beihang UniversityBeijing 100191China School of Chemistry Sun Yat-sen UniversityGuangzhou 510006China State Key Laboratory of Metal Matrix Composites Shanghai Jiao Tong UniversityShanghai 200240China Key Laboratory of Semiconductor Materials Science Beijing Key Laboratory of Low Dimensional Semiconductor Materials and DevicesInstitute of SemiconductorsChinese Academy of SciencesBeijing 100083China

Bandgap-graded materials present varying spectral responses at different positions,making them possible to be used as an alternative to photoactive materials array in multi-spectral responsive devices,thus miniaturizing the ***,the preparation of bandgap-graded materials usually requires complicated deposition *** we report a facile lowtemperature solution process to make films with lateral bandgap gradients,which form spontaneously via self-spreading and interdiffusion of *** show lead halide perovskite films with MAPbCl_(3)-MAPbBr_(3)and MAPbBr_(3)-MAPbI_(3)gradients,which exhibit light absorption onsets ranging from 410 to 781 *** bandgap-graded films were used to make self-powered multiband photodetectors,which show different spectral responses at different positions without applying bias ***,self-powered spectrometers were made by using the multiband photodetectors.

关键词： self-spreading interdiffusion bandgap-graded perovskite multiband photodetectors self-powered spectrometers

Effects on the STAT3-shRNA in Non-Small-Cell Lung Cancer Therapy: Design, Induction of Apoptosis, and Conjugation with Chitosan-Based Gene Vectors

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Journal of Ocean University of China 2021年第5期20卷 1097-1108页

作者： ZHANG Wangwang ZHANG yan JIANG zhiwen SUN Le WANG Litong GU Zhiyang LI Wenya GUO Lili CHEN Xiaotong ZHANG Haibin HAN Baoqin CHANG Jing College of Marine Life Science Ocean University of ChinaQingdao 266003China Laboratory for Marine Drugs and Bioproducts Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266235China

STAT3 plays a particularly important role in several cancer-related signal transduction *** STAT3 via RNA interference or small molecule inhibitors induces the apoptosis of tumor cells,thereby inhibiting the growth of the *** this study,short-hairpin RNA sequences targeting the STAT3 genes were designed,synthesized,and then connected to pGPU6/GFP/Neo plasmids as the shRNA-expression *** expression of STAT3-shRNA was analyzed by real-time PCR,western blotting,and cell apoptosis assay to study the growth and apoptosis of the ***,the effect of STAT3 knockdown on the NCI-H1650 cells was studied in a tumor mouse *** results revealed that,after an in vitro transfection,the proliferation of NCI-H1650 cells was inhibited,and the cells were induced to *** mRNA and protein expression levels of STAT3 were downregulated in the STAT3-shRNA *** vivo,the tumor mass and volume in the STAT3-shRNA group were significantly lower than in the other two *** the in vivo and in vitro results demonstrated a long-period inhibiting effect on NSCLC,especially in vivo,when the tumor inhibition rate could reach 50%in the STAT3-shRNA group,which is an exciting ***,the study of the conjugation of STAT3-shRNA and chitosan-based vectors revealed that they could be combined steadily with good cytocompati-bility and transfection *** results together provide convincing evidence for the application of STAT3-shRNA used in the treatment of non-small lung cancer,which could be a promoting prospect for the development of gene therapy.

关键词： signal transducer and activator of transcription 3 short-hairpin RNA target downregulation chitosan-based vectors gene therapy

Conserved immuno-collagenic subtypes predict response to immune checkpoint blockade

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Cancer Communications 2024年第5期44卷 554-575页

作者： Jie Mei Yun Cai Rui Xu Qing Li Jiahui Chu zhiwen Luo Yaying Sun Yuxin Shi Junying Xu Di Li Shuai Liang Ying Jiang Jiayu Liu zhiwen Qian Jiaofeng Zhou Mengyun Wan Yunlong yang Yichao Zhu yan Zhang Yongmei Yin Department of Oncology The First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsuP.R.China The First Clinical Medicine College Nanjing Medical UniversityNanjingJiangsuP.R.China Departments of Gynecology Wuxi Maternal and Child Health Care HospitalWuxi Medical CenterNanjing Medical UniversityWuxiJiangsuP.R.China Departments of Oncology Xuzhou Central HospitalThe Xuzhou School of Clinical Medicine of Nanjing Medical UniversityXuzhouJiangsuP.R.China Department of Sports Medicine Huashan Hospital Affiliated to Fudan UniversityShanghaiP.R.China Department of Sports Medicine Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghaiP.R.China Departments of Oncology The AffiliatedWuxi People’s Hospital of Nanjing Medical UniversityWuxi Medical CenterNanjing Medical UniversityWuxiJiangsuP.R.China Shanghai Outdo Biotech Co. Ltd.National Engineering Center for BiochipShanghaiP.R.China Departments of Gynecology Wuxi Maternity and Child Health Care HospitalAffiliatedWomen’s Hospital of Jiangnan UniversityWuxiJiangsuP.R.China Department of Physiology School of Basic Medical SciencesNanjing Medical UniversityNanjingJiangsuP.R.China Department of Cellular and Genetic Medicine School of Basic Medical SciencesFudan UniversityShanghaiP.R.China Jiangsu Key Lab of Cancer Biomarkers Prevention and TreatmentCollaborative Innovation Center for Personalized Cancer MedicineNanjing Medical UniversityNanjingJiangsuP.R.China

Background:Immune checkpoint blockade(ICB)has revolutionized the treatment of various cancer *** significant preclinical advancements in understanding mechanisms,identifying the molecular basis and predictive biomarkers for clinical ICB responses remains *** evidence,both preclinical and clinical,underscores the pivotal role of the extracellular matrix(ECM)in modulating immune cell infiltration and *** study aimed to create an innovative classifier that leverages ECM characteristics to enhance the effectiveness of ICB ***:We analyzed transcriptomic collagen activity and immune signatures in 649 patients with cancer undergoing ICB *** analysis led to the identification of three distinct immuno-collagenic subtypes predictive of ICB *** validated these subtypes using the transcriptome data from 9,363 cancer patients from The Cancer Genome Atlas(TCGA)dataset and 1,084 inhouse ***,novel therapeutic targets were identified based on these established immuno-collagenic ***:Our categorization divided tumors into three subtypes:“soft&hot”(low collagen activity and high immune infiltration),“armored&cold”(high collagen activity and low immune infiltration),and“quiescent”(low collagen activity and immune infiltration).Notably,“soft&hot”tumors exhibited the most robust response to ICB therapy across various cancer ***,inhibiting collagen augmented the response to ICB in preclinical ***,these subtypes demonstrated associations with immune activity and prognostic predictive potential across multiple cancer ***,an unbiased approach identified B7 homolog 3(B7-H3),an available drug target,as strongly expressed in“armored&cold”tumors,relating with poor ***:This study introduces histopathology-based universal immunocollagenic subtypes capable of predicting ICB responses across diverse cancer *** findings offer insights

关键词： collagen deposition immune infiltration immunotherapy pan-cancer tumor microenvironment

带防撞栏杆扁平箱梁高阶模态涡激振动的CFD研究

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振动与冲击 2021年第10期40卷 228-234,277页

作者：祝志文石亚光颜爽汕头大学土木与环境工程系广东汕头515063 中冶南方工程技术有限公司武汉430000

为研究大跨度桥梁常用扁平箱梁带防撞栏杆时的高阶模态涡激振动(VIV)特性,基于雷诺时均Navier-Stokes(RANS)方程、SST k-ω湍流模型和动网格技术求解扁平箱梁的绕流场并获得气动力,将Newmark-β算法代码嵌入到用户自定义函数(UDF)求解... 详细信息

为研究大跨度桥梁常用扁平箱梁带防撞栏杆时的高阶模态涡激振动(VIV)特性,基于雷诺时均Navier-Stokes(RANS)方程、SST k-ω湍流模型和动网格技术求解扁平箱梁的绕流场并获得气动力,将Newmark-β算法代码嵌入到用户自定义函数(UDF)求解该气动力作用下的桥梁动力响应,开展了大带东桥主桥加劲梁断面高阶模态涡激振动响应的预测,模拟雷诺数为3.18×10^(4)~6.10×10^(4)。获得了扁平箱梁断面随折减风速变化的高阶涡振响应振幅根方差(RMS)曲线和加速度时程,预测了与文献较一致的高阶模态涡激振动锁定区间。研究了阻尼比和来流风攻角对扁平箱梁高阶模态涡振响应幅值和加速度的影响,表明随着阻尼比的增大高阶模态涡激振动响应逐渐变小甚至消失,而来流风攻角只有大于2°时才会发生显著的高阶模态涡激振动。

关键词：大跨度桥梁涡激振动(VIV) 计算流体动力学(CFD) 扁平箱梁高阶模态流固耦合