检索结果-南通市图书馆

Generation and characterization of nanobodies targeting GPCR

在线全文

学校读者我要写书评

暂无评论

Biophysics Reports 2024年第1期10卷 22-30页

作者： Shenglan zhang zhiran Fan Jianfeng Liu Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory) Cellular Signaling laboratory International Research Center for Sensory Biology and Technology of MOST Key Laboratory of Molecular Biophysics of MOE College of Life Science and Technology Huazhong University of Science and Technology

G protein-coupled receptors(GPCRs) are a large family of cell membrane proteins that are important targets for drug discovery. Nanobodies, also known as VHH(variable domains of heavy chain-only antibodies, HcAbs) antibodies, are small antibody fragments derived from camelids that have gained significant attention as potential therapeutics for targeting GPCRs due to their advantages over conventional antibodies. However, there are challenges in developing nanobodies targeting GPCRs, among which epitope accessibility is the most significant because the cell membrane partially shields the GPCR surface. We developed a universal protocol for making nanobodies targeting GPCRs using the cell membrane extract of GPCR-overexpressing HEK293 cells as the llama/alpaca immunization antigen. We constructed an immune VHH library and identified nanobodies by phage display bio-panning. The monoclonal nanobodies were recombinantly expressed in Escherichia coli(E. coli) and purified to characterize their binding potency.

关键词： Nanobody Single domain antibody Heavy chain-only antibody GPCR G protein-coupled receptor

Effects of carbon content on the microstructure and tensile properties of a low-density steel

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 391-401页

作者： Yongxuan Shang Mingyu Fan Shuyong Jiang Zhongwu zhang Key Laboratory of Superlight Materials and Surface Technology Ministry of EducationCollege of Materials Science and Chemical EngineeringHarbin Engineering UniversityHarbin 150001China State Key Laboratory of Metal Material for Marine Equipment and Application Iron&Steel Research Institute of Ansteel Group CorporationAnshan 114009China

Carbon can change the phase components of low-density steels and influence the mechanical *** this study,a new method to control the carbon content and avoid the formation ofδ-ferrite by decarburization treatment was *** microstructural changes and mechanical characteristics with carbon content induced by decarburization were systematically ***-Jaoul(C-J)analysis was employed to examine the work hardening characteristics during the tensile *** decarburization by heat treatments,the carbon content within the austenite phase decreased,while Mn and Al were almost unchanged;this made the steel with full austenite transform into the austenite and ferrite dual ***,(Ti,V)C carbides existed in both matrix phase and the mole fraction almost the *** addition,the formation of other carbides *** loss induced a decrease in strength due to the weakening of the carbon solid *** the steel with the single austinite,the deformation mode of austenite was the dislocation planar glide,resulting in the formation of *** the dual-phase steel,the deformation occurred by the dislocation planar glide of austenite first,with the increase in strain,the cross slip of ferrite took place,forming dislocation cells in *** the late stage of deformation,the work hardening of austinite increased rapidly,while that of ferrite increased slightly.

关键词： low-density steels carbon content decarburization strengthening mechanisms work hardening behavior

Dielectric-ion-conductive ZnNb2O6 layer enabling rapid desolvation and diffusion for dendrite-free Zn metal batteries

在线全文

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2025年第1期100卷 693-701页

作者： Haifeng Yang Jian Wang Panpan zhang Xiaomin Cheng Qinghua Guan Jing Dong Bixian Chen Lujie Jia Jing zhang Yongzheng zhang Yunjian Liu Hongzhen Lin School of Materials Science and Engineering Jiangsu University i-Lab & CAS Key Laboratory of Nanophotonic Materials and Devices Suzhou Institute of Nano-Tech and Nano-Bionics Chinese Academy of Sciences Helmholtz Institute Ulm (HIU) Karlsruhe Institute of Technology (KIT) School of Materials Science and Engineering Xi'an University of Technology State Key Laboratory of Chemical Engineering East China University of Science and Technology

Rechargeable aqueous zinc-metal batteries （AZMBs） are promising candidates for large-scale energy storage systems due to their low cost and high ***,their performance and sustainability are significantly hindered by the sluggish desolvation kinetics at the electrode/electrolyte interface and the corresponding hydrogen evolution reaction where active water molecules tightly participate in the Zn（H2O）62+solvation ***,learnt from self-generated solid electrolyte interphase （SEI） in anodes,the dielectric but ion-conductive zinc niobate nanoparticles artificial layer is constructed on metallic Zn surface （ZNB@Zn）,acting as a rapid desolvation *** zincophilic and dielectric-conductive properties of ZNB layer accelerate interfacial desolvation/diffusion and suppress surface corrosion or dendrite formation,achieving uniform Zn plating/stripping behavior,as confirmed by electronic/optical microscopies and interface spectroscopical measurements together with theoretical ***,the as-prepared ZNB@Zn electrode exhibits excellent cycling stability of over 2000 h and robust reversibility （99.54%） even under high current density and depth of discharge ***,the assembled ZNB@Zn-based full cell displays high capacity-retention rate of 80.21%after 3000 cycles at 5 A g-1and outstanding rate performance up to 10 A *** large-areal pouch cell is stabilized for hundreds of cycles,highlighting the bright prospects of the dielectric but ion-conductive layer in further application of AZMBs.

关键词： Zn metal battery Dielectric artificial layer Rapid ion diffusion Zincophilic diffusion Active water inhibition

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

葡萄酒感官质量评价及其理化因子分析

现代食品 2022年第7期28卷 202-206页

作者：张志然王恩辉李兴元许程夏磊烟台市标准计量检验检测中心山东烟台264003 国家葡萄酒及白酒、露酒产品质量检验中心山东烟台264003

我国葡萄酒企业尚未形成统一的感官质量分级体系,使消费者无法直观辨别产品质量的好坏。以往感官质量评价多注重于直观感受描述而忽略其背后理化因子的相关性,无法帮助企业进行产品质量提升。本文通过对比不同葡萄酒产品感官评品数据与... 详细信息

我国葡萄酒企业尚未形成统一的感官质量分级体系,使消费者无法直观辨别产品质量的好坏。以往感官质量评价多注重于直观感受描述而忽略其背后理化因子的相关性,无法帮助企业进行产品质量提升。本文通过对比不同葡萄酒产品感官评品数据与挥发酸、二氧化硫、甘油以及花青素含量这些理化因子的关联性,发现过高的挥发酸、二氧化硫可以在一定程度影响葡萄酒产品的感官评价,为鉴别葡萄酒质量高低,以及产品质量升级提供数据支撑。

关键词：葡萄酒感官质量理化因子相关性分析

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

关于葡萄酒中检出微量甜蜜素的探讨

中外葡萄与葡萄酒 2018年第5期 68-72页

作者：赵永福张志然宋吉利朱济义张书文国家葡萄酒及白酒露酒产品质量监督检验中心山东烟台264003 全国酿酒标准化技术委员会葡萄酒分技术委员会山东烟台264003 烟台市产品质量监督检验所山东烟台264003

国家标准规定食品添加剂甜蜜素在葡萄酒中不得添加。葡萄酒中甜蜜素新旧检测方法,由于定量限的不同,导致检出结果、判定结果不同,给许多葡萄酒生产企业带来困扰。本文通过对葡萄酒中检出微量甜蜜素进行探讨,认为检出微量甜蜜素就判定为... 详细信息

国家标准规定食品添加剂甜蜜素在葡萄酒中不得添加。葡萄酒中甜蜜素新旧检测方法,由于定量限的不同,导致检出结果、判定结果不同,给许多葡萄酒生产企业带来困扰。本文通过对葡萄酒中检出微量甜蜜素进行探讨,认为检出微量甜蜜素就判定为添加并不合理。甜蜜素检测方法标准更新,相关判定标准之间理应保持协调一致,需完善判定标准,对葡萄酒中甜蜜素规定科学合理的阈值。不断规范葡萄酒行业相关的法规及标准,保障葡萄酒行业的健康发展。

关键词：葡萄酒甜蜜素不得检出不得添加

在线全文

学校读者我要写书评

暂无评论

雌激素与肺通气功能关系概述

国际呼吸杂志 2015年第13期35卷 1026-1028页

作者：屈超刘中洋张治然袭荣刚王晓波沈阳军区药物研究所大连116015 解放军第二一○医院药剂科大连116015

肺是呼吸系统的重要组成器官，多种遗传或者环境因素可以导致肺癌、肺水肿、肺气肿等严重疾病。雌激素主要在卵巢、肾上腺中合成。雌激素通过与雌激素受体ERα、ERβ及GPR30结合参与人体生理、病理的调节。在肺中，雌激素主要参与胎儿... 详细信息

肺是呼吸系统的重要组成器官，多种遗传或者环境因素可以导致肺癌、肺水肿、肺气肿等严重疾病。雌激素主要在卵巢、肾上腺中合成。雌激素通过与雌激素受体ERα、ERβ及GPR30结合参与人体生理、病理的调节。在肺中，雌激素主要参与胎儿肺泡形成和肺泡表面积维持，且与气管生理具有密切的关系。更重要的是，雌激素广泛存在于肺的组织细胞中，且肺的形成、生理功能、疾病的发生均与性别有关。因此，了解雌激素与肺通气活动之间的关系对于阐述肺部疾病发病的机制具有重要的意义。

关键词：雌激素雌激素受体肺生理肺泡

Synergistically enhanced ORR and HER performance on Co-N-C coupled in-situ generated PtCo intermetallic

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2025年第1期100卷 721-729页

作者： Guanyu Luo Min Song Lulu An Xiao Huang Qian zhang Chenhao zhang Tao Shen Shuang Wang Deli Wang Key Laboratory of Material Chemistry for Energy Conversion and Storage (Huazhong University of Science and Technology) Ministry of Education School of Chemistry and Chemical Engineering Huazhong University of Science and Technology

Integrating multi-scale sites in a composite catalyst is vital to realize efficient ***,a synergistic composite catalyst consisting of Co atomic sites and in-situ generated PtCo intermetallic compounds （IMCs）(o-PtCo@CoNC) is proposed through Co pre-anchoring and subsequent impregnation-reduction *** loading of Co atoms provides a chance for in-situ generating PtCo ordered intermetallic *** remaining Co single atoms and PtCo IMCs construct synergistic electrocatalytic *** from the ordered structure,synergistic effect of Pt Co IMCs and Co single atoms,o-PtCo@CoNC exhibits excellent electrocatalytic performance for oxygen reduction reaction （ORR） and hydrogen evolution reaction （HER） with mass activity of 1.21 A mgPt-1（at 0.9 V） and5.70 A mgPt-1（at an overpotential of 100 mV）,***,o-PtCo@CoNC delivers negligible loss of half-wave potential and overpotential during long-term stability test in acid solutions,with 13 mV decay after 50,000 potential cycles for ORR and a 2.7 mV decay after 20,000 potential cycles for *** integration strategy of single-atomic sites coupled IMCs paves the way for enhancing the activity and durability of Pt-based electrocatalysts.

关键词： Electrolytic reduction

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

正交试验优化纳米雄黄温敏凝胶基质处方

辽宁中医药大学学报 2015年第8期17卷 52-54页

作者：李奕诺赵宇王晓波张治然袭荣刚辽宁中医药大学药学院辽宁大连116600 哈尔滨医科大学药学院黑龙江哈尔滨150081 解放军第210医院药理基地辽宁大连116015

目的:探索温敏凝胶药物制剂基质中辅料泊洛沙姆407(P407)、泊洛沙姆188(P188)、1,2-丙二醇(PG)对胶凝温度和黏度的影响,从而考察辅料的性能及其用量,制备出纳米雄黄温敏凝胶制剂。方法:采用正交设计,选取P407、P188和PG的浓度为考察因素... 详细信息

目的:探索温敏凝胶药物制剂基质中辅料泊洛沙姆407(P407)、泊洛沙姆188(P188)、1,2-丙二醇(PG)对胶凝温度和黏度的影响,从而考察辅料的性能及其用量,制备出纳米雄黄温敏凝胶制剂。方法:采用正交设计,选取P407、P188和PG的浓度为考察因素,以胶凝温度、黏度和溶解度的综合评分为指标,优化纳米雄黄温敏凝胶的基质处方。结果:优选的基质处方为20%的P407、1%的P188和10%的PG。结论:按优选的基质处方制得的纳米雄黄温敏凝胶制剂黏度和胶凝温度良好。

关键词：纳米雄黄温敏凝胶正交设计胶凝温度黏度

不同前处理方法测定儿童即食海苔中铝的残留量及其风险评估

在线全文

学校读者我要写书评

暂无评论

现代食品 2021年第18期6卷 182-185页

作者：张志然王恩辉张璎鸾柳杨徐方正杨兵成烟台市标准计量检验检测中心山东烟台264003

本文采用湿法消解、干法消解以及微波消解3种前处理方法,利用电感耦合等离子发射光谱仪(ICP)测定市面在售11种儿童即食海苔中铝的含量,结果表明,微波消解法的消解效率最佳,适合处理儿童即食海苔样品。进口儿童即食海苔中铝含量普遍高于... 详细信息

本文采用湿法消解、干法消解以及微波消解3种前处理方法,利用电感耦合等离子发射光谱仪(ICP)测定市面在售11种儿童即食海苔中铝的含量,结果表明,微波消解法的消解效率最佳,适合处理儿童即食海苔样品。进口儿童即食海苔中铝含量普遍高于国产海苔,但按10 g每日摄入儿童即食海苔其铝的摄入安全边界比值小于1,说明铝的摄入量在人体可接受范围内,但幼儿(1~3岁)仍存在平均铝摄入量风险,建议相关部门在加强监管的同时应跟进限量标准的全面性。

关键词：即食海苔铝的残留量风险评估