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High temperature treatment induced production of unreduced 2n pollen in Camellia oleifera

Horticultural Plant Journal 2024年第3期10卷 879-896页

作者： Hongda Deng Xiaoyu Zhang Song Sheng Yuxun Huang Ziqi Ye Tongyue Zhang Xing Liu zhiqiang han The Laboratory of Forestry Genetics Central South University of Forestry and TechnologyChangshaHunan 410004China Yuelushan Laboratory ChangshaHunan 410128China Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education Central South University of Forestry and TechnologyChangshaHunan 410004China National Forest and Seedling Workstation of Hunan Province The Forestry Department of Hunan ProvinceChangshaHunan 410004China

Unreduced gametes through chromosome doubling play a major role in the process of plant polyploidization.Our previous work confirmed that Camellia oleifera can produce natural 2n pollen,and it is possible to induce the 2n pollen formation by high temperature treatment.This study focused on the optimization of the 2n pollen induction technique and the mechanisms of high temperature-induced2n pollen formation in C.oleifera.We found that the optimal protocol for inducing 2n pollen via high temperature was to perform 45℃with4 h at the prophaseⅠstage of the pollen mother cells(PMCs).Meanwhile,high temperature significantly decreased the yield and fertility of2n pollen.Through the observation of meiosis,abnormal chromosome and cytological behaviour was discovered under high-temperature treatment,and we confirmed that the formation of 2n pollen is caused by abnormal cell plate.Based on weighted gene co-expression network analysis,fifteen hub genes related to cell cycle control were identified.After male flower buds were exposed to heat shock,polygalacturonase gene(CoPGX3)was significantly upregulated.We inferred that high temperature causes the CoPGX3 gene to be overexpressed and that CoPGX3 is redistributed into the cytosol where it degrades cytoplasmic pectin,which leads to an abnormal cell plate.Furthermore,abnormal cytokinesis resulted in the formation of dyads and triads,and PMCs divided to produce 2n pollen.Our findings provide new insights into the mechanism of 2n pollen induced by high temperature in a woody plant and lay a foundation for further ploidy breeding of C.oleifera.

关键词： Camellia oleifera Pollen mother cells(PMCs) High temperature Meiosis 2n pollen CoPGX3

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Improve meat production and virus resistance by simultaneously editing multiple genes in livestock using Cas12i^(Max)

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Science China(Life Sciences) 2024年第3期67卷 555-564页

作者： Jilong Ren Tang Hai Yangcan Chen Ke Sun zhiqiang han Jing Wang Chongyang Li Qingwei Wang Leyun Wang Huabing Zhu Dawei Yu Wei Li Shanjiang Zhao State Key Laboratory of Animal Nutrition Key Laboratory of Animal GeneticsBreeding and Reproduction of Ministry of Agriculture and Rural AffairsInstitute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193China State Key Laboratoryf Stem Cell and Reproductive Biology Instituteof ZooogyChinese Academyof SciencesBeijing 100101China Beijing Institute for Stem Cell and Regenerative Medicine Beijing 100101China Beijing Farm Animal Research Center Institute of ZoologyChinese Academy of SciencesBeijing 100101China

The clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated gene(Cas) system is continually optimized to achieve the most efficient gene editing effect. The Cas12i^(Max), a Cas12i variant, exhibits powerful DNA editing activity and enriches the gene editing toolbox. However, the application of Cas12i^(Max)in large domestic animals has not yet been reported. To verify the efficiency and feasibility of multiple gene editing in large animals, we generated porcine fibroblasts with simultaneous knockouts of IGF2, ANPEP, CD163,and MSTN via Cas12i^(Max)in one step. Phenotypically stable pigs were created through somatic cell nuclear transfer technology. They exhibited improved growth performance and muscle quality. Furthermore, we simultaneously edited three genes in bovine fibroblasts. A knockout of MSTN and PRNP was created and the amino acid Q-G in CD18 was precisely substituted. Meanwhile, no off-target phenomenon was observed by sum-type analysis or off-target detection. These results verified the effectiveness of Cas12i^(Max)for gene editing in livestock animals and demonstrated the potential application of Cas12i^(Max)in the field of animal trait improvement for agricultural production.

关键词： Cas12i^(Max) gene editing livestock multiple trait improvement

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Full-length transcripts facilitates Portunus trituberculatus genome structure annotation

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Journal of Oceanology and Limnology 2022年第5期40卷 2042-2051页

作者： Fangrui LOU zhiqiang han School of Ocean Yantai UniversityYantai 264005China Fishery College Zhejiang Ocean UniversityZhoushan 316022China

Portunus trituberculatus is an ide al model for elucidating crustacean genetic networks.Here we combined single molecule real-time(SMRT)sequencing and Illumina RNA-seq to characterize the coding genes,non-coding RNAs and pseudogenes and further to improve the genome annotation information of P.tritub erculatus.In this study,we assembled 9694 non-redundancy full-length transcripts,and 658737307-bp repetitive sequences were identified in the P.trituberculatus full-length transcriptome.We also predicted the P.tritub erculatus genome structure based on full-length transcripts,including 18602 genes,28686 non-coding RNAs,1407 pseudogenes,740 motif,and 26434 domain.Meanwhile,14460,10211,5412,7314,and 14448 genes had significant matches with sequences in the NR,KOG,GO,KEGG,and TrEMBL database,respectively.Overall,our work firstly provided the long-read transcriptome and we believed that these data are very necessary to improve the annotation information of P.trituberculatus genome structure,and useful information for the future studies on evolution and physiological regulation of P.trituberculatus.

关键词： Portunus trituberculatus full-length transcripts single molecule real-time(SMRT)sequencing

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Eff ects of climate change on the potential habitat distribution of swimming crab Portunus trituberculatus under the species distribution model

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Journal of Oceanology and Limnology 2022年第4期40卷 1556-1565页

作者： Xingyu LIU Xiaolu han zhiqiang han Fishery College Zhejiang Ocean UniversityZhoushan 316002China

Over the last decades,the species distribution model(SDM)has become an essential tool for studying the potential eff ects of climate change on species distribution.In this study,an ensemble SDM was developed to predict the changes in species distribution of swimming crab Portunus trituberculatus across diff erent seasons in the future(2050s and 2100s)under the climate scenarios of Representative Concentration Pathway(RCP)4.5 and RCP8.5.Results of the ensemble SDM indicate that the distribution of this species will move northward and exhibit evident seasonal variations.Among the four seasons,the suitable habitat for this species will be signifi cantly reduced in summer,with loss rates ranging from 45.23%(RCP4.5)to 88.26%(RCP.8.5)by the 2100s.The loss of habitat will mostly occur in the East China Sea and the southern part of the Yellow Sea,while a slight increase in habitat will occur in the northern part of the Bohai Sea.These fi ndings provide an information forecast for this species in the future.Such forecast will be helpful in improving fi shery management under climate change.

关键词： Portunus trituberculatus climate change species distribution model suitable habitat

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Unexpected complex horizontal gene transfer in teleost fish

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Current Zoology 2023年第2期69卷 222-223页

作者： zhiqiang han Shengyong Xu Tianxiang Gao Fishery College Zhejiang Ocean UniversityZhoushanZhejiang 316002China

Horizontal gene transfer(HGT)is a common occurrence across all domains of life.However,most HGT events were reported between single-celled organisms or parasites and hosts(Van Etten and Bhattacharya 2020).A type II antifreeze protein(AFP)gene was the first and sole evidence of HGT direct vertebrate-to-vertebrate DNA transmission.AFP is only found in 3 widely separated branches of teleost fishes(herring,sea raven,and smelts),sharing amino acid similarity up to 80%(Graham et al.2008).

关键词： horizontal gene transfer teleost fish

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Immune checkpoint therapy for solid tumours: clinical dilemmas and future trends

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Signal Transduction and Targeted Therapy 2023年第9期8卷 4188-4213页

作者： Qian Sun Zhenya Hong Cong Zhang Liangliang Wang zhiqiang han Ding Ma Department of Obstetrics and Gynecology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430030China Cancer Biology Research Center(Key Laboratory of the Ministry of Education) Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430030China Department of Hematology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430030China

Immune-checkpoint inhibitors(ICBs),in addition to targeting CTLA-4,PD-1,and PD-L1,novel targeting LAG-3 drugs have also been approved in clinical application.With the widespread use of the drug,we must deeply analyze the dilemma of the agents and seek a breakthrough in the treatment prospect.Over the past decades,these agents have demonstrated dramatic efficacy,especially in patients with melanoma and non-small cell lung cancer(NSCLC).Nonetheless,in the field of a broad concept of solid tumours,non-specific indications,inseparable immune response and side effects,unconfirmed progressive disease,and complex regulatory networks of immune resistance are four barriers that limit its widespread application.Fortunately,the successful clinical trials of novel ICB agents and combination therapies,the advent of the era of oncolytic virus gene editing,and the breakthrough of the technical barriers of mRNA vaccines and nano-delivery systems have made remarkable breakthroughs currently.In this review,we enumerate the mechanisms of each immune checkpoint targets,associations between ICB with tumour mutation burden,key immune regulatory or resistance signalling pathways,the specific clinical evidence of the efficacy of classical targets and new targets among different tumour types and put forward dialectical thoughts on drug safety.Finally,we discuss the importance of accurate triage of ICB based on recent advances in predictive biomarkers and diagnostic testing techniques.

关键词： clinical breakthrough dilemma

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Removal of the main inflorescence to induce reflowering of loquat

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Horticultural Plant Journal 2022年第1期8卷 35-43页

作者： Jiangrong Peng Wenkun Li Yuan Yuan zhiqiang han Yunpeng Cao Muhammad Qasim Shahid Zhike Zhang Yongshun Gao Shunquan Lin Yuanyuan Jiang Henry Fok College of Biology and Agriculture Shaoguan UniversityShaoguanGuangdong 512005China State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources College of HorticultureSouth China Agricultural UniversityGuangzhou 510642China The Key Laboratory of Cultivation and Protection for Non-wood Forest trees Ministry of EducationCentral South University of Forestry and TechnologyChangsha 410004China College of Agriculture South China Agricultural UniversityGuangzhou 510642China Beijing Engineering Research Center for Strawberry Beijing 100093China Beijing Academy of Forestry and Pomology Sciences Beijing 100093China

Loquat(Eriobotrya japonica Lindl.)is an evergreen fruit tree species of the Rosaceae,and its unique flowering time greatly hinders its production.To explore the artificial regulation of loquat flowering time,we removed the main inflorescence(by cutting it)to induce reflowering.For different loquat tree cultivars with different stages,the inflorescence was removed by cutting the main floral axis at two alternative positions:the upper or the lower position beneath the inflorescence,and it was found that the proportion of reflowering resulting from removing the upper position of the main floral axis of yellow-flesh loquat cultivars during the full-bloom stage was the highest.In addition,compared with those of the normal-growing panicles,the number of flower buds and branch axes of the reflowering panicles decreased significantly after cutting.Importantly,these newly produced inflorescences flowered 2–4 months later than normal-growing inflorescences did,effectively prolonging both the flowering and fruiting time.In addition,qRT-PCR results showed that EjFT1,EjFT2,EjAP1–1 and EjAP1–2 were highly expressed in the floral axis.These findings highlighted a new method for extending the production cycle of loquat and provided a reference for the flowering regulation of loquat and other economically important fruit tree species.

关键词： Eriobotrya japonica Reflowering Flowering time Cutting Production cycle

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Study on Dendritic Growth in Pressurized Solidification of Mg–Al Alloy Using Phase Field Simulation

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Journal of Materials Science & Technology 2016年第1期32卷 68-75页

作者： Haowei Pan zhiqiang han Baicheng Liu Key Laboratory for Advanced Materials Processing Technology Ministry of EducationSchool of Materials Science and EngineeringTsinghua University State Key Laboratory of Automotive Safety and Energy Department of Automotive EngineeringTsinghua University

A multi-grain phase field model coupled with thermodynamic calculation was adopted to describe the dendritic growth in pressurized solidification of Mg-A1 alloy during squeeze casting, in which the effects of the pressure on the Gibbs free energy and chemical potential of solid and liquid phases, the solute diffusion coefficient, and the solute partition coefficient were considered. The individual effect of solute diffusion coefficient, and the Gibbs free energy on the dendritic growth was studied. With the compar- ison of the dendritic growth under atmospheric and elevated pressures, the effect of pressure on the microstructure evolution was discussed. The results showed that the grains are refined, the dendritic growth rate tends to increase and the secondary dendrite arms are more developed as the pressure is increased from 0.1 to 100 MPa, which showed a good agreement with the experimental results of direct squeeze casting of Mg-AI alloy. As the pressure increases, the largest dendritic growth rate can be obtained under the pressure between 200 and 250 MPa, while the growth rate decreases with a further increase of pressure.

关键词： Phase field Pressurized solidification Dendritic growth Mg-Al alloy Gibbs free energy

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计算机数据融合算法下植保机精准化作业研究

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农机化研究 2024年第1期46卷 107-111页

作者：韩志强九州职业技术学院江苏徐州221116

针对我国传统的植保机在精准化作业和自动化喷洒方面存在的不足,基于计算机数据融合算法对植保机精准化作业进行了设计试验。植保机的主要组成包括控制单元模块、信息采集模块、传感器模块、数据传输模块和变量执行器模块。为了实现植... 详细信息

针对我国传统的植保机在精准化作业和自动化喷洒方面存在的不足,基于计算机数据融合算法对植保机精准化作业进行了设计试验。植保机的主要组成包括控制单元模块、信息采集模块、传感器模块、数据传输模块和变量执行器模块。为了实现植保机的精准化作业,对植保机的飞行姿态调整和变量喷药过程进行了算法设计。其中,飞行姿态控制采用双闭环位置式PID控制算法,喷药过程采用改进的遗传PID控制算法进行控制。为了验证植保机精准化作业的效果,对其进行姿态控制和变量喷药试验。结果表明:植保机姿态控制良好,能够完成精准化喷药。

关键词：植保机精准化作业数据融合算法双闭环位置式PID控制算法改进遗传PID控制算法

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石英挠性加速度计力矩器仿真建模与优化设计

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中国惯性技术学报 2024年第5期32卷 492-498,510页

作者：许哲徐啸韩志强陈崇陈泓玮上海机电工程研究所上海201109

石英挠性加速度计中磁场性能直接影响加速度计输出精度,而力矩器是影响加速度计内部磁场的关键。因此,以石英挠性加速度计中力矩器为研究对象,采用等效磁路方法建立含补偿环元件的力矩器工作气隙磁场数学模型,以此为基础分析工作气隙磁... 详细信息

石英挠性加速度计中磁场性能直接影响加速度计输出精度,而力矩器是影响加速度计内部磁场的关键。因此,以石英挠性加速度计中力矩器为研究对象,采用等效磁路方法建立含补偿环元件的力矩器工作气隙磁场数学模型,以此为基础分析工作气隙磁场均匀性和磁场强度等性能,并采用多参数优化方法对力矩器系统进行整体优化。仿真结果表明,优化模型有效提升力矩器工作气隙磁场均匀性,磁场轴向极差ΔB_(R)由386.3396 mT缩小至108.2755 mT,端点极差ΔB_(D)由217.8894 mT缩小至2.1934 mT,线圈位置气隙轴向磁场几乎保持对称,从而降低加速度计输出非线性,验证了优化方案的可行性。

关键词：石英挠性加速度计力矩器等效磁路磁场均匀性多参数优化

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