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Acta Oceanologica Sinica 2017年第6期36卷 52-60页

作者： QIAO Ying WANG Jun MAO Yong LIU Min SONG Xiaohong su Yongquan WANG Chunzhong ZHENG zhipeng State Key Laboratory of Marine Environmental Sciences Xiamen UniversityXiamen 361102China Putian Tian-ran-xing Agricultural Development Co.Ltd. Fujian 351100China

Cathepsin L gene is a member of the cysteine proteinase gene group. In this study Cathepsin L gene was isolated from Kuruma shrimp Marsupenaeusjaponicus （Mj-Cathepsin L） and the full-length DNA sequence was 1 963 bp. Mj-Cathepsin L protein showed high homologies with other Cathepsin L proteins documented in vertebrates, mollusks and other crustaceans. Expression analysis of Mj-Cathepsin L gene in different tissues revealed that it was predominant in hepatopancreas. During early ontogenetic development stages Mj-Cathepsin L showed a development-regulated expression, and the Mj-Cathepsin L showed a molting stage-regulated expression during the five molting stages, inferring its role in the ontogenic development of ***. Two kinds of forms of Mj- Cathepsin L protein： pro-Cathepsin L and Cathepsin L were measured in hepatopancreas, stomach and intestine by Western Blotting.

关键词： Cathepsin L gene larval development molting cycle tissue distribution Marsupenaeusjaponicus

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复合掠弯轴流增压设计技术

航空学报 2024年第5期45卷 317-335页

作者：曹志鹏王永明赵龙波关朝斌牛潇陈晨中国航发四川燃气涡轮研究院成都610500

提高增压比是未来涡扇发动机实现低耗油率需求的关键途径,而级数少、重量轻、长度短的高负荷轴流增压系统一直是研究重点。小展弦比复合掠弯能够有效控制超声速流动损失以及大折转角下的流动分离,是高负荷设计技术的重点发展方向。本文... 详细信息

提高增压比是未来涡扇发动机实现低耗油率需求的关键途径,而级数少、重量轻、长度短的高负荷轴流增压系统一直是研究重点。小展弦比复合掠弯能够有效控制超声速流动损失以及大折转角下的流动分离,是高负荷设计技术的重点发展方向。本文在大前掠带箍风扇基础上,开展了高负荷两级风扇超跨声速级间流动匹配研究,发展了全新的小展弦比、三维掠弯设计方法;基于试验结果完成了一维、二维特性模型修正,采用优化方法建立了全工况性能匹配调节规律,并完成了优化调节试验验证。以此为基础,为进一步提高负荷,开展小展弦比串列叶片前段减速增压、后段折转增压概念原理设计和试验验证;运用数值模拟方式进行了动叶自循环吸附验证;完成了低反力度气动布局设计和静叶吸附的试验验证。最后,初步讨论了可变流量Flade叶片构型面临的高效率全超声速动叶设计、环内壁低损失超声速流动控制等技术挑战,以及柔性叶片和智能材料相融合的造型设计方法。

关键词：小展弦比复合掠弯串列叶片自循环吸附低反力度 Flade叶片柔性叶片

几何修形对盾构机主驱动轴承润滑性能的影响分析

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机床与液压 2024年第8期52卷 140-146页

作者：连士林苏建新杨宏斌郭义凯李林昌郭晓欣梁志鹏河南科技大学机电工程学院河南洛阳471000

以某6 m盾构机实际工况及其主驱动轴承为研究对象,建立主驱动轴承载荷分布模型及等温弹流润滑模型,得到了各工况下的负载受力,分析了几何修形对盾构机主轴承弹流润滑的影响。结果表明:从弹流润滑角度判断,滚子母线凸度及端部圆角半径均... 详细信息

以某6 m盾构机实际工况及其主驱动轴承为研究对象,建立主驱动轴承载荷分布模型及等温弹流润滑模型,得到了各工况下的负载受力,分析了几何修形对盾构机主轴承弹流润滑的影响。结果表明:从弹流润滑角度判断,滚子母线凸度及端部圆角半径均存在优化区间,从而使最大滚子负载处的油膜压力显著减小,减小边缘效应;几何修形后的滚子明显比直母线滚子的润滑性能要好,且修形后的滚子在工况变化时,存在自适应性,但极限工况下仍存在油膜压力过大和油膜厚度偏低的情况。因此当极限工况时间占比较大时,应适当增加滚子凸度。综上可知,合理选取几何修形滚子参数可显著提高盾构机主驱动轴承润滑性能。

关键词：弹流润滑几何修形油膜厚度油膜压力

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浅析美国国家鱼类与野生生物基金会

海洋经济 2024年第1期14卷 78-83页

作者：刘佳吕慧铭王志鹏夏颖颖苏冠先国家海洋信息中心天津300171

充分利用社会资本参与生态保护修复、增强生态产品供给和社区韧性、促进蓝色经济发展,是美国政府广泛使用的政策工具之一,这种方式有效缓解了财政资金压力。通过介绍美国国家鱼类与野生生物基金会的组织结构、资金来源、项目管理、伙伴... 详细信息

充分利用社会资本参与生态保护修复、增强生态产品供给和社区韧性、促进蓝色经济发展,是美国政府广泛使用的政策工具之一,这种方式有效缓解了财政资金压力。通过介绍美国国家鱼类与野生生物基金会的组织结构、资金来源、项目管理、伙伴关系等情况,并以国家海岸带韧性基金为例,分析其典型案例和成功经验。

关键词：基金美国 PPP模式生态保护修复

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猪链球菌生物被膜研究进展

畜牧兽医科学（电子版） 2022年第9期 148-149页

作者：叶思齐朱佳文苏志鹏郑云龙贾德凯曹雨辰西南大学动物医学院重庆400700 成都市农林科学院畜牧研究所成都四川611130 四川德康农牧食品集团股份有限公司成都四川610200

猪链球菌(Streptococcus suis,SS)是重要的病原菌,引发宿主出现化脓性脑膜炎、败血症、心内膜炎、肺炎和关节炎等疾病。生物被膜的形成是导致猪链球菌致病性和耐药性增加的主要原因,深入了解猪链球菌生物被膜的调控系统和形成因素对猪... 详细信息

猪链球菌(Streptococcus suis,SS)是重要的病原菌,引发宿主出现化脓性脑膜炎、败血症、心内膜炎、肺炎和关节炎等疾病。生物被膜的形成是导致猪链球菌致病性和耐药性增加的主要原因,深入了解猪链球菌生物被膜的调控系统和形成因素对猪链球菌病的防治有重要意义,另外,luxS基因可能会是未来抗菌药物的新靶标之一。

关键词：猪链球菌生物被膜调控系统形成因素防控技术

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蟋蟀后肠纤维素降解细菌的分离与鉴定

生物资源 2020年第2期42卷 228-233页

作者：张科苏智鹏许阳李清利王瑜刘丽李冰洁周云霞夏西超平顶山学院医学院河南平顶山467000 汝州市畜牧局河南汝州467599

近年来,具有农业、能源和环保价值的昆虫微生物种类和基因得到了开发,昆虫肠道微生物展示了其巨大的应用潜力,本研究旨在从蟋蟀后肠分离和鉴定纤维素降解细菌。首先采用羧甲基纤维素钠液体培养基对蟋蟀后肠中的微生物进行富集培养,然后... 详细信息

近年来,具有农业、能源和环保价值的昆虫微生物种类和基因得到了开发,昆虫肠道微生物展示了其巨大的应用潜力,本研究旨在从蟋蟀后肠分离和鉴定纤维素降解细菌。首先采用羧甲基纤维素钠液体培养基对蟋蟀后肠中的微生物进行富集培养,然后使用羧甲基纤维素钠固体培养基分离和筛选单菌落,再通过16S rRNA测序对纤维素降解细菌进行分子鉴定,最后通过刚果红染色来进一步分析细菌降解纤维素的能力。从蟋蟀后肠中共分离出20株纤维素降解细菌,16S rRNA基因测序结果显示来自肠杆菌属(Enterobacter)9株,不动杆菌属(Acinetobacter)7株,克雷伯氏菌属(Klebsiella)2株,鞘氨醇杆菌属(Sphingobacterium)1株和葡萄球菌属(Staphylococcus)1株。刚果红染色试验结果显示,克雷伯氏菌属两株PDSCDXS2B和8B,鞘氨醇杆菌属PDSCDXS7C和不动杆菌属PDSCDXS12C具有较高的纤维素降解能力。这是首次从蟋蟀后肠分离和筛选出来具有纤维素降解能力的细菌,为昆虫源纤维素降解细菌的研究提供了微生物资源。

关键词：蟋蟀纤维素降解细菌 16S rRNA

miR-17-5p在大鼠垂体泌乳素腺瘤MMQ细胞耐药中的作用

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温州医科大学学报 2019年第4期49卷 258-262,280页

作者：管佳清许家栋王春勇苏志鹏蔡霖陈贤斌郑伟明温州医科大学附属第一医院神经外科浙江温州325015

目的:探讨microRNA-17-5p(mi R-17-5p)在大鼠垂体泌乳素腺瘤MMQ细胞耐药中的作用及相关机制。方法:利用miR-17-5p类似物及拮抗物分别过表达和抑制大鼠垂体泌乳素腺瘤MMQ细胞中miR-17-5p的表达,并采用实时荧光定量PCR(RT-q PCR)法进行检... 详细信息

目的:探讨microRNA-17-5p(mi R-17-5p)在大鼠垂体泌乳素腺瘤MMQ细胞耐药中的作用及相关机制。方法:利用miR-17-5p类似物及拮抗物分别过表达和抑制大鼠垂体泌乳素腺瘤MMQ细胞中miR-17-5p的表达,并采用实时荧光定量PCR(RT-q PCR)法进行检测。利用CCK-8法检测miR-17-5p表达调控前后细胞增殖以及对卡麦角林(CAB)治疗反应的变化。通过生物信息学方法预测miR-17-5p的靶点基因,然后采用荧光素酶报告检测法进行验证,继而用qRT-PCR及Westernblot法检测miR-17-5p对靶基因表达的影响。慢病毒细胞转染过表达MMQ细胞中的PTEN基因,用Westernblot检测PTEN的表达水平,并采用CCK-8法检测PTEN过表达对MMQ细胞耐药性的影响。结果:mi R-17-5p过表达可促进MMQ细胞的增殖,而miR-17-5p敲减则能抑制MMQ细胞的增殖。mi R-17-5p敲减能上调MMQ细胞对CAB治疗的敏感性,相反,miR-17-5p过表达则显著降低MMQ细胞对CAB治疗的敏感性。生物信息学方法预测PTEN为miR-17-5p潜在作用靶点,荧光素酶报告检测法进一步验证了两者的相互作用关系,同时miR-17-5p过表达的MMQ细胞中PTENmRNA及蛋白表达量显著下降,而miR-17-5p敲减的MMQ细胞中PTENmRNA及蛋白表达量显著上升。PTEN表达上调能部分逆转miR-17-5p过表达引起的MMQ细胞对CAB治疗敏感性降低。结论:miR-17-5p可通过下调PTEN的表达促进MMQ细胞增殖并降低其对CAB治疗的反应。

关键词： microRNA-17-5p 泌乳素瘤卡麦角林 PTEN 大鼠

Emerging organic contaminants of liquid crystal monomers:Environmental occurrence,recycling and removal technologies,toxicities and health risks

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Eco-Environment & Health 2023年第3期2卷 131-141页

作者： zhipeng Cheng Shaohan Zhang Huijun su Haoduo Zhao Guanyong su Mingliang Fang Lei Wang MOE Key Laboratory of Pollution Processes and Environmental Criteria College of Environmental Science and EngineeringNankai UniversityTianjin 300350China Key Laboratory of Environmental Remediation and Ecological Health Ministry of Industry and Information TechnologySchool of Environmental and Biological EngineeringNanjing University of Science and TechnologyNanjing 210094China Department of Environmental Science and Engineering The University of North Carolina at Chapel Hill135 Dauer DriveChapel HillNC 27599USA Department of Environmental Science and Engineering Fudan UniversityShanghai 200433China

Liquid crystal monomers(LCMs)are a family of synthetic organic chemicals applied in the liquid crystal displays(LCDs)of various electric and electronic products(e-products).Due to their unique properties(i.e.,persistence,bioaccumulative potential,and toxicity)and widespread environmental distributions,LCMs have attracted increasing attention across the *** studies have focused on the source,distribution,fate,and toxicity of LCMs;however,a comprehensive review is ***,we highlighted the persistence and bioaccumulation potential of LCMs by reviewing their physical–chemical *** naming rules were suggested to standardize the abbreviations regarding *** sources and occurrences of LCMs in different environmental compartments,including dust,sediment,soil,leachate,air and particulate,human serum,and biota samples,were *** is concluded that the LCMs in the environment mainly originate from the usage and disassembly of eproducts with ***,the review of the potential recycling and removal technologies regarding LCMs from waste LCD panels suggests that a combination of natural attenuation and physic-chemical remediation should be developed for LCMs remediations in the *** reviewing the health risks and toxicity of LCMs,it is found that a large gap exists in their toxicity and risk to *** fate and toxicity investigation of LCMs,and further investigations on the effects on the human exposure risks of LCMs to residents,especially to occupational workers,should be considered in the future.

关键词： Liquid crystal monomers Occurrences Removal Human exposure Toxicity

Holey graphene oxide-templated construction of nano nickel-based metal–organic framework for highly efficient asymmetric supercapacitor

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Nano Research 2022年第10期15卷 9047-9056页

作者： Hang Wang Pengfei Zhao Xingmao Zhang su Zhang Xiaolong Lu zhipeng Qiu Kang Ren Zheng Xu Ruxin Yao Tong Wei Zhuangjun Fan State Key Laboratory of Heavy Oil Processing School of Materials Science and EngineeringChina University of PetroleumQingdao 266580China Key Laboratory of Magnetic Molecules&Magnetic Information Materials(Ministry of Education) Institute of Chemistry and CultureSchool of Chemistry&Material ScienceShanxi Normal UniversityTaiyuan 030006China

Metal–organic frameworks(MOFs)with redox-active metal sites and controllable crystalline structures make it possible to access the merits of highly-efficient electrode materials in electrochemical energy storage ***,most MOFs suffer from low capacitance and poor cycling stability that largely thwart their ***,we present the holey graphene oxide(HGO)template strategy to prepare nano two-dimensional Ni(BDC)with HGO as both template and capping agent(denoted as Ni(BDC)-HGOx,x=10,20,30,and 40 according to the added HGO amount).Structural analyses reveal that HGO can significantly inhibit the Ni(BDC)agglomeration,thus offering a high ion-accessible surface ***(BDC)-HGO30 with well-exposed active sites exhibits a high capacitance of 1,115.6 F·g^(−1) at 1 A·g^(−1) in 6 M KOH aqueous,1.8 times that of bulk Ni(BDC).An asymmetric supercapacitor with Ni(BDC)-HGO30 as a positive electrode and activated carbon as the opposing electrode delivers an energy density of 52.5 W·h·kg^(−1) and a power density up to 18.0 kW·kg−1,with 92.5%capacitance retention after 10,000 *** intermittent titration technique and in situ electrochemical–Raman measurements were exploited to elucidate the electrochemical behavior of Ni(BDC)-*** results pave the way for the development of rationally tuned MOF materials for enhancing supercapacitor performances.

关键词： holey graphene oxide templated construction nano MOFs supercapacitor

A comprehensive review of azadirachtin: physicochemical properties, bioactivities, production, and biosynthesis

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Acupuncture and Herbal Medicine 2023年第4期3卷 256-270页

作者： Xinyao su zhipeng Liang Qiang Xue Jia Liu Xuemi Hao Caixia Wang School of Chinese Materia Medica Tianjin University of Traditional Chinese MedicineTianjinChina Institute of Chinese Materia Medica China Academy of Chinese Medical SciencesBeijingChina College of Life Sciences Huaibei Normal UniversityHuaibeiChina

Azadirachtin,a complex tetratriterpenoid limonin with potent insecticidal properties,is the most widely used biological pesticide *** versatile pharmacological applications include the inhibition of tumor growth and anti-malarial,anti-bacterial,and anti-inflammatory *** plays a pivotal role in pest control and novel drug *** primary source of azadirachtin is the neem tree(Azadirachta indica ***),with an azadirachtin content ranging from 0.3%to 0.5%.Despite the market demand for botanical pesticides reaching approximately 100,000 tons per year,the annual neem production in China is only 1.14 *** azadirachtin can be obtained through plant extraction or chemical synthesis,the quantity obtained does not meet the market demand in *** sluggish pace of azadirachtin biosynthesis results from the limited availability of genetic information and the complexity of the synthetic *** advancements in azadirachtin biosynthesis hold promise as an efficient collection *** this study,we explored the physicochemical properties,biological activities,mechanisms of action,and acquisition methods of *** also delved into recent progress in azadirachtin biosynthesis and assessed potential future usage *** study aims to establish a theoretical foundation for the scientific application and efficient synthesis of azadirachtin,offering valuable reference information to the industry.

关键词： Azadirachtin Biosynthesis Mechanism of action Neem Graphical abstract:http://***/AHM/A79