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Analysis of Roadbed Pavement Designs for Highway Reconstruction and Upgrading

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Journal of Architectural Research and Development 2024年第3期8卷 93-97页

作者： Haonan Ding zhipan yang Wei Gan China Merchants Chongqing Communications Technology Research&Design Institute Co. LTD.Chongqing 400067China

This paper focuses on the route and roadbed pavement design in highway reconstruction and upgrading projects.It discusses the importance of project design for highway reconstruction and upgrading,highlighting key aspects of route design and roadbed pavement design.The analysis reveals that the main design considerations in these projects include controlling factors of route reconstruction,expansion,and upgrading,as well as route plan design and longitudinal section design combined with roadbed pavement.In roadbed pavement design,it is crucial for designers to thoroughly collect existing data and make reasonable use of the current roadbed and pavement to develop a comprehensive design scheme.This analysis aims to provide a reference for the reasonable design of such projects.

关键词： Highway engineering Reconstruction and upgrading design Route design Roadbed and pavement design

Autophagy targets Hd1 for vacuolar degradation to regulate rice flowering

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Molecular Plant 2022年第7期15卷 1137-1156页

作者： Zhi Hu zhipan yang Yi Zhang Aihong Zhang Qingtao Lu Ying Fang Congming Lu Key Laboratory of Photobiology Institute of BotanyChinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Crop Biology College of Life ScienceShandong Agricultural UniversityTaianShandong 271018China

Flowering time(heading date)is a critical agronomic trait that determines the yield and regional adaptability of crops.Heading date 1(Hd1)is a central regulator of photoperiodic flowering in rice(Oryza sativa).However,how the homeostasis of Hd1 protein is achieved is poorly understood.Here,we report that the nuclear autophagy pathway mediates Hd1 degradation in the dark to regulate flowering.Loss of autophagy function results in an accumulation of Hd1 and delays flowering under both short-day and long-day conditions.In the dark,nucleus-localized Hd1 is recognized as a substrate for autophagy and is subjected to vacuolar degradation via the autophagy protein OsATG8.The Hd1-0sATG8 interaction is required for autophagic degradation of Hd1 in the dark.Our study reveals a new mechanism by which Hd1 protein homeostasis is regulated by autophagy to control rice flowering.Our study also indicates that the regulation of flowering by autophagic degradation of Hd1 orthologs may have arisen over the course of mesangiosperm evolution,which would have increased their flexibility and adaptability to the environment by modulating flowering time.

关键词： autophagy heading date rice Hd1 homeostasis

Pentatricopeptide repeat protein PHOTOSYSTEM I BIOGENESIS FACTOR2 is required for splicing of ycf3

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Journal of Integrative Plant Biology 2020年第11期62卷 1741-1761页

作者： Xuemei Wang zhipan yang Yi Zhang Wen Zhou Aihong Zhang Congming Lu Key Laboratory of Ph otobiology Institute of BotanyChinese Academy of SciencesBeijing 100093China College of Life Sciences U niversity of C hinese Academy of SciencesBeijing 100049China State Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityTaian 271018China

To gain a better understanding of the molec-ular mechanisms of photosystem!(PSI)biogenesis,wecharacterized the Arabidopsis thaliana photosystem l bio-genesis factor 2(pbf2)mutant,which lacks PSl complex.PBF2 encodes a P-class pentatricopeptide repeat(PPR)protein.In the pbf2 mutants,we observed a striking de-crease in the transcript level of only one gene,thechloroplast gene ycf3,which is essential for PSl assembly.Further analysis of ycf3 transcripts showed that PBF2 isspecifically required for the splicing of ycf3 intron 1.Computational prediction of binding sequences and elec-trophoretic mobility shift assays reveal that PBF2 specifi-cally binds to a sequence in ycf3 intron 1.Moreover,we found that PBF2 interacted with two general factors forgroup ll intron splicing CHLOROPLAST RNA SPLICING2-AsSOCIATED FACTOR1(CAF1)and CAF2,and facilitated theassociation of these two factors with ycf3 intron 1.Ourresults suggest that PBF2 is specifically required for thesplicing of ycf3 intron 1 through cooperating with CAF1and CAF2.Our results also suggest that additional proteinsare required to contribute to the specificity of CAF-dependent group ll intron splicing.

关键词： complex. striking specificity

Analysis of Highway Subgrade Slope Protection and Support Technologies

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Journal of World Architecture 2023年第4期7卷 1-5页

作者： zhipan yang Haonan Ding Shuai Li China Merchants Chongqing Communications Technology Research&Design Institute Co. LTD.Chongqing 400067China

As an important transportation hub in China,the traffic volume and driving speed are important aspects of expressways.Therefore,the protection requirements for roadbed side slopes are higher,and it is necessary to resist rainwater erosion and other damages by protecting the side slopes.Therefore,it is necessary to adopt effective technical means of subgrade protection and support.This paper mainly summarizes the characteristics of highway subgrade slope protection construction and slope protection and support technologies.

关键词： Expressway Embankment slope Protection Support technology

Application Strategy of Green Transportation Concept in Urban Road Design

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Journal of World Architecture 2023年第5期7卷 1-5页

作者： Haonan Ding zhipan yang Shuai Li China Merchants Chongqing Communications Technology Research&Design Institute Co. Ltd.Chongqing 400067China

Urban traffic and urban environmental pollution are important issues that must be taken into account in the process of urbanization,especially in China.Modernization has enabled many developed countries in the west to carry out rational city traffic planning.At the same time,the concept of green transportation has also emerged,integrating low-energy consumption,low pollution,comfort,and safety into urban traffic to effectively solve issues pertaining to traffic and pollution.The concept of green transportation has been widely adopted in China and made popular in many cities,thereby promoting the sustainable development of cities.

关键词： Green transportation concept Urban road design Public transport

Decreased glutathione reductase2 leads to early leaf senescence in Arabidopsis

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Journal of Integrative Plant Biology 2016年第1期58卷 29-47页

作者： Shunhua Ding Liang Wang zhipan yang Qingtao Lu Xiaogang Wen Congming Lu Photosynthesis Research Center Key Laboratory of Photobiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China University of Chinese Academy of Sciences Beijing 100049 China

Glutathione reductase（GR） catalyzes the reduction of glutathione disulfide（GSSG） to reduced glutathione（GSH）and participates in the ascorbate-glutathione cycle, which scavenges H_2O_2. Here, we report that chloroplastic/mitochondrial GR2 is an important regulator of leaf senescence. Seed development of the homozygous gr2 knockout mutant was blocked at the globular stage. Therefore, to investigate the function of GR2 in leaf senescence, we generated transgenic Arabidopsis plants with decreased GR2 using RNAi. The GR2 RNAi plants displayed early onset of age-dependent and darkand H2O2-induced leaf senescence, which was accompanied by the induction of the senescence-related marker genes SAG12 and SAG13. Furthermore, transcriptome analysis revealed that genes related to leaf senescence, oxidative stress, and phytohormone pathways were upregulated directly before senescence in RNAi plants. In addition, H2O2 accumulated to higher levels in RNAi plants than in wild-type plants and the levels of H_2O_2 peaked in RNAi plants directly before the early onset of leaf senescence. RNAi plants showed a greater decrease in GSH/GSSG levels than wild-type plants during leaf development. Our results suggest that GR2 plays an important role in leaf senescence by modulating H_2O_2 and glutathione signaling in Arabidopsis.

关键词： Arabidopsis thaliana ascorbate-glutathione cycle glutathione reductase2 hydrogen peroxide leaf senescence

Analysis of Interchange Exit Safety Technology

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Journal of World Architecture 2023年第4期7卷 6-10页

作者： Shuai Li Zhonglei Yu zhipan yang China Merchants Chongqing Communications Technology Research&Design Institute Co. LTD.Chongqing 400067China

In order to improve the safety of the exit of expressway interchanges,the main problems and influencing factors of interchange exits are analyzed according to the number of traffic accidents at expressway interchange exits in China.Some suggestions and countermeasures are then put forward from the aspect of safety technology for future reference.

关键词： Interchange Exit safety Safety guarantee

The Phytol Phosphorylation Pathway Is Essential for the Biosynthesis of Phylloquinone, which Is Required for Photosystem I Stability in Arabidopsis

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Molecular Plant 2017年第1期10卷 183-196页

作者： Lei Wang Qingwei Li Aihong Zhang Wen Zhou Rui Jiang zhipan yang Huixia yang Xiaochun Qin Shunhua Ding Qingtao Lu Xiaogang wen Congming Lu Photosynthesis Research Center Key Laboratory of Photobiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China University of Chinese Academy of Sciences Beijing 100049 China National Center for Plant Gene Research Beijing 100093 China

Phytyl-diphosphate, which provides phytyl moieties as a common substrate in both tocopherol and phyllo- quinone biosynthesis, derives from de novo isoprenoid biosynthesis or a salvage pathway via phytol phos- phorylation. However, very little is known about the role and origin of the phytyl moiety for phylloquinone biosynthesis. Since VTE6, a phytyl-phosphate kinase, is a key enzyme for phytol phosphorylation, we char- acterized Arabidopsis vte6 mutants to gain insight into the roles of phytyl moieties in phylloquinone biosyn- thesis and of phylloquinone in photosystem I （PSI） biogenesis. The VTE6 knockout mutants vte6-1 and vte6-2 lacked detectable phylloquinone, whereas the phylloquinone content in the VTE6 knockdown mutant vte6-3 was 90% lower than that in wild-type. In vte6 mutants, PSI function was impaired and accu- mulation of the PSI complex was defective. The PSI core subunits PsaA/B were efficiently synthesized and assembled into the PSI complex in vte6-3. However, the degradation rate of PSI subunits in the assembled PSI complex was more rapid in vte6-3 than in wild-type. In vte6-3, PSI was more susceptible to high-light damage than in wild-type. Our results provide the first genetic evidence that the phytol phosphorylation pathway is essential for phylloquinone biosynthesis, and that phylloquinone is required for PSI complex stability.

关键词： phytol phosphorylation VTE6 phylloquinone PSI stability

Wavelength injection locking actively Q-switched random fiber laser based on random phase-shifted fiber Bragg grating and electro-optic modulator

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Optoelectronics Letters 2024年第3期20卷 135-141页

作者： PAN Honggang ZHANG Bo SONG Dianyou CHEN zhipan CHEN Chunqi LI Rupeng School of Integrated Circuit Science and Engineering Tianjin University of TechnologyTianjin 300384China

In this paper, an actively Q-switched wavelength injection locking random fiber laser(RFL) based on random phase-shifted fiber Bragg grating(RPS-FBG) is proposed, and the performance of the laser is verified by experiments. Within the reflection bandwidth range of RPS-FBG, spanning from 1 549.2 nm to 1 549.9 nm, different laser modes with stable central wavelength and peak power can be selectively chosen by varying the injected light wavelength. The power fluctuation within 1 h is less than 0.1 d Bm, and the central wavelength drift is less than 0.02 nm. When the pump power increases from 90 mW to 300 mW, the pulse width decreases from 3.2 μs to 1.5 μs, and the pulse repetition frequency is 20 kHz. The RFL can reach a stable locking state at the lowest pump power of 100 mW and the lowest injection power of 3 d Bm. When the wavelength is locked, the output pulse is a single pulse. On the contrary, the unlocked output pulse is multi-pulse. The laser has the characteristics of high wavelength tunability in the reflection range of RPS-FBG and it can be an ideal light source in the fields of laser imaging and pulse coding.

关键词： fiber switched modulator