检索结果-南通市图书馆

在线全文

学校读者我要写书评

暂无评论

榼藤子对组胺急性痒小鼠的治疗作用研究

中南民族大学学报（自然科学版） 2024年第4期43卷 461-467页

作者：鞠炎锟和金凤闫婷熊慧梅之南中南民族大学药学院武汉430074

为了研究榼藤子提取物对组胺急性痒小鼠的治疗作用,通过组胺诱导小鼠急性痒模型,并将造模成功的小鼠随机分为模型组,榼藤子提取物低(25.0 mg/kg)、中(50.0 mg/kg)、高(100.0 mg/kg)剂量组,阳性药苯海拉明组(10.0 mg/kg).结果表明:榼藤... 详细信息

为了研究榼藤子提取物对组胺急性痒小鼠的治疗作用,通过组胺诱导小鼠急性痒模型,并将造模成功的小鼠随机分为模型组,榼藤子提取物低(25.0 mg/kg)、中(50.0 mg/kg)、高(100.0 mg/kg)剂量组,阳性药苯海拉明组(10.0 mg/kg).结果表明:榼藤子提取物可以降低组胺急性痒小鼠挠痒次数;明显改善皮肤表皮增厚;减少炎性细胞浸润;减少肥大细胞数量.此外,榼藤子提取物可以下调皮肤中H1R、TRPV1、IL-4、IL-6、IL-1β、IL-33、TNF-α、TSLP以及DRG中痒相关因子受体的基因表达水平,降低皮肤中H1R,TSLP与IL-4的蛋白含量.榼藤子提取物可以明显缓解组胺急性痒小鼠的症状,可能与其下调痒相关因子的基因与蛋白表达水平有关.

关键词：榼藤子组胺急性痒炎性因子

维普期刊数据库博看期刊

Spectroscopic and dynamic light scattering studies of the interaction between pterodontic acid and bovine serum albumin

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2012年第1期2卷 53-59页

作者： Yunfang Li Guangzhong Yang zhinan mei College of Pharmacy South Central University for NationalitiesWuhan 430074China

Pterodontic acid(PA)has been isolated from Laggera pterodonta,a Chinese herbal medicine,and shown to possess antibacterial activity in *** facilitate its preclinical development,the interaction between PA and bovine serum albumin(BSA)was studied using a fluorescence quenching technique,ultraviolet–visible spectrophotometry and dynamic light scattering(DLS).At temperatures of 297 K and 310 K and an excitation wavelength of 282 nm,the fluorescence intensity of BSA decreased significantly with increasing concentration of PA attributed to the formation of a PA–BSA *** apparent binding constant,number of binding sites and corresponding thermodynamic parameters were calculated and the main intermolecular attraction shown to result from hydrogen bonding and van der Waals *** fluorescence spectrometry revealed that the binding site in the complex approached the microenvironment of Trp and three-dimensional fluorescence spectroscopy showed the binding induced conformational changes in *** DLS,increasing PA concentration was shown to cause a gradual increase in hydrodynamic diameter and significant aggregation of the complex.

关键词： Pterodontic acid Bovine serum albumin Fluorescence quenching Synchronous fluorescence Three-dimensional fluorescence Dynamic light scattering

A telomere-to-telomere reference genome provides genetic insight into the pentacyclic triterpenoid biosynthesis in Chaenomeles speciosa

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Research 2023年第10期10卷 250-261页

作者： Shaofang He Duanyang Weng Yipeng Zhang Qiusheng Kong Keyue Wang Naliang Jing Fengfeng Li Yuebin Ge Hui Xiong Lei Wu De-Yu Xie Shengqiu Feng Xiaqing Yu Xuekui Wang Shaohua Shu zhinan mei College of Plant Science&Technology Huazhong Agricultural UniversityWuhan 430070China Wuhan Carboncode Biotechnologies Co. Ltd.Wuhan 430070China Sinopharm Zhonglian Pharmaceutical Co. Ltd.Wuhan 430070China College of Horticulture&Forestry Huazhong Agricultural UniversityWuhan 430070China School of Pharmaceutical Science South-Central Minzu UniversityWuhan 430074China Department of Plant and Microbial Biology North Carolina State UniversityRaleighNC 27695USA College of Horticulture Nanjing Agricultural UniversityNanjing 210095China

Chaenomeles speciosa(2n=34),a medicinal and edible plant in the Rosaceae,is commonly used in traditional Chinese *** date,the lack of genomic sequence and genetic studies has impeded efforts to improve its medicinal ***,we report the use of an integrative approach involving PacBio HiFi(third-generation)sequencing and Hi-C scaffolding to assemble a high-quality telomere-to-telomere genome of *** genome comprised 650.4 Mb with a contig N50 of 35.5 *** these,632.3 Mb were anchored to 17 pseudo-chromosomes,in which 12,4,and 1 pseudo-chromosomes were represented by a single contig,two contigs,and four contigs,*** pseudo-chromosomes had telomere repeats at both ends,and four had telomere repeats at a single *** sequences accounted for 49.5%of the genome,while a total of 45515 protein-coding genes have been *** genome size of *** was relatively similar to that of Malus *** or contracted gene families were identified and investigated for their association with different plant metabolisms or biological *** particular,functional annotation characterized gene families that were associated with the biosynthetic pathway of oleanolic and ursolic acids,two abundant pentacyclic triterpenoids in the fruits of *** together,this telomere-to-telomere and chromosome-level genome of *** not only provides a valuable resource to enhance understanding of the biosynthesis of medicinal compounds in tissues,but also promotes understanding of the evolution of the Rosaceae.

关键词： value. insight metabolism

Application of microcalorimetry of Escherichia coli growth and discrimin antanalysis to the quality assessment of a Chinese herbal injection(Yinzhihuang)

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2012年第3期2卷 278-285页

作者： Yongshen Ren Ping Zhang Dan Yan Yan Yan Longhu Chen Lingling Qiu zhinan mei Xiaohe Xiao China Military Institute of Chinese Materia Medica 302 Military Hospital of ChinaBeijing 100039China Pharmacy College South-Central University for NationalitiesWuhan 430074China

This paper describes a novel approach to the quality control of a Chinese herbal injection based on microca lorimetric determination of its effect on the biothermal active fingerprint(BTAF)of Escherichia coli(***)*** Injection(YZHI)was selected for this proof of concept *** samples of Y ZHI were collected and compared with test(stressed)samples prepared under different stress *** BTAF of *** growth was found to be affected by YZHI and the changes were analyzed on the basis of eleven biothermokinetic *** and multivariate statistical analysis were used to investi-gate the differences between reference and test samples and discriminant analysis was used to delineate the altered *** samples were found to have coincident BTAFs with similarity index>*** samples showed differences in the BTAF which increased in line with decreased *** formulae were developed based on a sensitivity parameter which could identify all altered *** conclusion,BTAF can be used to assess the quality of YZHI both qualitatively and quantitatively and has the potential to provide a sensitive method for quality control of Chinese herbal injections.

关键词： Biothermal active fingerprint Microcalorimetry Quality control Chinese herbal injection Escherichia coli

The anti-tumor effects of flavonoids from the ethnic medicine Docycia delavayi(Franch.)*** its possible mechanism

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The anti-tumor effects of flavonoids from the ethnic medicin...

科技引领产业、支撑跨越发展——第六届湖北科技论坛

作者： Xukun Deng Xiangpei Zhao Lvyi Chen Ze yang Zhou zhinan mei Qinxiong Lin School of Pharmacy South-Central University for Nationalities

Docycia delavayi(Franch.) Schneid.,a Chinese Dai ethnic medicine popularly known as "Duoyi" in Yunnan province of China,had been traditionally used as a folk remedy for fever,cancer,fracture and beriberoid disease for a long time in *** present study was carried out to investigate the active components and the anti -tumor effect mechanism of the flavonoids including chrysin,quercetin,naringenin and avicularin from Docycia delavayi(Franch.) Schneid..The MTT-assay showed that the inhibitory activity of chrysin was much stronger than the other three flavonoids on HepG2 cells,EC109 cells,Hela cells and SW480 cells for 48 h treatment in vitro,with the IC50 values of 73.7,85.1,114.7,106.3μM,*** study revealed that chrysin exhibited the strong suppressive effect on HepG2 in concentration- and time-dependent *** addition,chrysin caused HepG2 cell shrinkage,membrane blebbing,apoptotic body formation,all of which are typical characteristics of apoptotic programmed cell *** results of flow cytometric analysis demonstrated that chrysin caused apoptosis of HepG2 cells through cell cycle arrest at G0/G1 phase,thus preventing cells entering S phase. Immunoblot results showed that chrysin significantly increased the expression levels of caspase-3 and Bax protein, and decreased the expression level of B-cell lymphoma/leukemia-2(Bcl-2) *** findings indicate that the major flavonoids present in Docycia delavayi(Franch.) *** inhibit the growth activity of tumor cells,among which chrysin introduce HepG2 cells death via apoptosis,probably through the participation of caspase-3,Bax and Bcl-2.

关键词： Docycia delavayi(Franch.) Schneid anti-tumor chrysin flavonoids HepG2 cells

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

胡颓子叶水溶性化学成分的研究

中国中药杂志 2012年第9期37卷 1224-1226页

作者：李孟顺廖矛川葛月宾梅之南中南民族大学药学院湖北武汉430074

目的:研究胡颓子叶水溶性部位的化学成分。方法:采用大孔树脂柱色谱,反相硅胶柱色谱,Sephadex LH-20柱色谱和半制备高效液相色谱分离纯化,通过理化性质和波谱鉴定化合物结构。结果:从胡颓子叶中分离出2个化合物,其结构分别鉴定为山柰酚-... 详细信息

目的:研究胡颓子叶水溶性部位的化学成分。方法:采用大孔树脂柱色谱,反相硅胶柱色谱,Sephadex LH-20柱色谱和半制备高效液相色谱分离纯化,通过理化性质和波谱鉴定化合物结构。结果:从胡颓子叶中分离出2个化合物,其结构分别鉴定为山柰酚-3-O-β-D-葡萄糖基-(1→3)-α-L-鼠李糖基-(1→6)-β-D-半乳糖苷[kaempferol 3-O-β-D-glucopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→6)-β-D-galactopyranoside,1],山柰酚-3-O-β-D-葡萄糖基-(1→3)-α-L-鼠李糖基-(1→6)-β-D-半乳糖苷-7-O-β-D-葡萄糖苷[kaempferol 3-O-β-D-glucopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→6)-β-D-galactopyranoside-7-O-β-D-glucopyranoside,2]。结论:从胡颓子叶中分离到的化合物2是一个新化合物。

关键词：胡颓子叶化学成分黄酮苷结构鉴定

在线全文

学校读者我要写书评

暂无评论

野花椒中2个新苯丙素成分的研究

有机化学 2013年第6期33卷 1345-1348页

作者：朱德荣祝丹陈玉梅之南杨光忠广东医学院药物化学与药分教研室东莞523808 中南民族大学药学院武汉430074 中南民族大学化学与材料科学学院武汉430074

运用正相、反相硅胶柱层析和HPLC对野花椒(Zanthoxylum utile Huang)的化学成分进行研究,并利用现代波谱技术鉴定化合物的结构.从野花椒茎木乙醇提取物中分离得到14个化合物,分别鉴定为robustine(1),香兰子酸(2),dihydroalatamide(3),te... 详细信息

运用正相、反相硅胶柱层析和HPLC对野花椒(Zanthoxylum utile Huang)的化学成分进行研究,并利用现代波谱技术鉴定化合物的结构.从野花椒茎木乙醇提取物中分离得到14个化合物,分别鉴定为robustine(1),香兰子酸(2),dihydroalatamide(3),tembamide(4),N-benzoyltyramine(5),blumenol(6),blumenol C(7),芥子醛(8),(E)-3-(3,4,5-三甲氧基苯基)-2-丙烯醇(9),松柏醇(10),integrifoliodiol(11),两面针氧新木脂素(12),以及两个新的苯丙素类成分,分别命名为野花椒甲素(13)和乙素(14).所有化合物为首次从该植物中分离得到.

关键词：花椒属野花椒苯丙素分离结构鉴定

在线全文

学校读者我要写书评

暂无评论

高效液相色谱法测定醋氯芬酸缓释片的含量

中国现代应用药学 2001年第1期18卷 53-55页

作者：王槐武黄励梅之南湖北黄石市国药有限公司黄石435000 大冶有色金属公司职工医院湖北医科大学附一院药学部

目的 :建立了高效液相色谱法测定醋氯芬酸缓释片的含量。方法 :采用ZorbaxSB C18色谱柱 ,以乙腈四氢呋喃冰醋酸(2 5∶2 5∶5 0 ,用 1.0mol/L的NaOH调pH 3.5 )为流动相 ,流速为 1.0ml/min ,以对羟基联苯为内标物 ,检测波长为 2 75nm。... 详细信息

目的 :建立了高效液相色谱法测定醋氯芬酸缓释片的含量。方法 :采用ZorbaxSB C18色谱柱 ,以乙腈四氢呋喃冰醋酸(2 5∶2 5∶5 0 ,用 1.0mol/L的NaOH调pH 3.5 )为流动相 ,流速为 1.0ml/min ,以对羟基联苯为内标物 ,检测波长为 2 75nm。结果 :醋氯芬酸在 10 .2～ 5 0 .1μg/ml范围内呈良好线性 (r=0 .9993) ,平均回收率为 10 0 .3% ,RSD为 0 .45 %。结论 :本法可用于该片剂的测定 ,操作简便 ,结果准确。

关键词：高效液相色谱法醋氯芬酸缓释片含量测定非甾体抗炎药

Profiling of two Chinese medicinal plants,Sophora flavescens and Ligusticum chuangxiong,by off-line LC-NMR and LC-MS

在线全文

学校读者我要写书评

暂无评论

Profiling of two Chinese medicinal plants,Sophora flavescens...